LOCUS       NM_022930               1933 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus cholinergic receptor nicotinic alpha 9 subunit
            (Chrna9), mRNA.
ACCESSION   NM_022930
VERSION     NM_022930.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Gallino,S.L., Aguero,L., Boffi,J.C., Schottlender,G.,
            Buonfiglio,P., Dalamon,V., Marcovich,I., Carpaneto,A., Craig,P.O.,
            Plazas,P.V. and Elgoyhen,A.B.
  TITLE     Key role of the TM2-TM3 loop in calcium potentiation of the
            alpha9alpha10 nicotinic acetylcholine receptor
  JOURNAL   Cell Mol Life Sci 81 (1), 337 (2024)
   PUBMED   39120784
  REMARK    GeneRIF: Key role of the TM2-TM3 loop in calcium potentiation of
            the alpha9alpha10 nicotinic acetylcholine receptor.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Boffi,J.C., Marcovich,I., Gill-Thind,J.K., Corradi,J., Collins,T.,
            Lipovsek,M.M., Moglie,M., Plazas,P.V., Craig,P.O., Millar,N.S.,
            Bouzat,C. and Elgoyhen,A.B.
  TITLE     Differential Contribution of Subunit Interfaces to alpha9alpha10
            Nicotinic Acetylcholine Receptor Function
  JOURNAL   Mol Pharmacol 91 (3), 250-262 (2017)
   PUBMED   28069778
  REMARK    GeneRIF: the contribution of subunit interface domains to
            alpha9alpha10 receptors by a combination of electrophysiological
            and radioligand binding studies, is reported.
            Erratum:[Mol Pharmacol. 2017 May;91(5):437. doi:
            10.1124/mol.111.107482err.. PMID: 28325810]
REFERENCE   3  (bases 1 to 1933)
  AUTHORS   Lipovsek,M., Fierro,A., Perez,E.G., Boffi,J.C., Millar,N.S.,
            Fuchs,P.A., Katz,E. and Elgoyhen,A.B.
  TITLE     Tracking the molecular evolution of calcium permeability in a
            nicotinic acetylcholine receptor
  JOURNAL   Mol Biol Evol 31 (12), 3250-3265 (2014)
   PUBMED   25193338
REFERENCE   4  (bases 1 to 1933)
  AUTHORS   Zouridakis,M., Giastas,P., Zarkadas,E., Chroni-Tzartou,D.,
            Bregestovski,P. and Tzartos,S.J.
  TITLE     Crystal structures of free and antagonist-bound states of human
            alpha9 nicotinic receptor extracellular domain
  JOURNAL   Nat Struct Mol Biol 21 (11), 976-980 (2014)
   PUBMED   25282151
REFERENCE   5  (bases 1 to 1933)
  AUTHORS   Azam,L. and McIntosh,J.M.
  TITLE     Molecular basis for the differential sensitivity of rat and human
            alpha9alpha10 nAChRs to alpha-conotoxin RgIA
  JOURNAL   J Neurochem 122 (6), 1137-1144 (2012)
   PUBMED   22774872
  REMARK    GeneRIF: A two order of magnitude species difference in potency of
            alpha-conotoxin RgIA is reported for rat versus human alpha9alpha10
            nAChR.
REFERENCE   6  (bases 1 to 1933)
  AUTHORS   Elgoyhen,A.B., Vetter,D.E., Katz,E., Rothlin,C.V., Heinemann,S.F.
            and Boulter,J.
  TITLE     alpha10: a determinant of nicotinic cholinergic receptor function
            in mammalian vestibular and cochlear mechanosensory hair cells
  JOURNAL   Proc Natl Acad Sci U S A 98 (6), 3501-3506 (2001)
   PUBMED   11248107
REFERENCE   7  (bases 1 to 1933)
  AUTHORS   Verbitsky,M., Rothlin,C.V., Katz,E. and Elgoyhen,A.B.
  TITLE     Mixed nicotinic-muscarinic properties of the alpha9 nicotinic
            cholinergic receptor
  JOURNAL   Neuropharmacology 39 (13), 2515-2524 (2000)
   PUBMED   11044723
REFERENCE   8  (bases 1 to 1933)
  AUTHORS   Glowatzki,E. and Fuchs,P.A.
  TITLE     Cholinergic synaptic inhibition of inner hair cells in the neonatal
            mammalian cochlea
  JOURNAL   Science 288 (5475), 2366-2368 (2000)
   PUBMED   10875922
REFERENCE   9  (bases 1 to 1933)
  AUTHORS   Katz,E., Verbitsky,M., Rothlin,C.V., Vetter,D.E., Heinemann,S.F.
            and Elgoyhen,A.B.
  TITLE     High calcium permeability and calcium block of the alpha9 nicotinic
            acetylcholine receptor
  JOURNAL   Hear Res 141 (1-2), 117-128 (2000)
   PUBMED   10713500
REFERENCE   10 (bases 1 to 1933)
  AUTHORS   Elgoyhen,A.B., Johnson,D.S., Boulter,J., Vetter,D.E. and
            Heinemann,S.
  TITLE     Alpha 9: an acetylcholine receptor with novel pharmacological
            properties expressed in rat cochlear hair cells
  JOURNAL   Cell 79 (4), 705-715 (1994)
   PUBMED   7954834
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On Nov 26, 2020 this sequence version replaced NM_022930.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U12336.1, AY574257.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760434 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-150               JAXUCZ010000014.1  42595520-42595669   c
            151-296             JAXUCZ010000014.1  42595139-42595284   c
            297-451             JAXUCZ010000014.1  42593840-42593994   c
            452-984             JAXUCZ010000014.1  42590807-42591339   c
            985-1933            JAXUCZ010000014.1  42588948-42589896   c
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p11"
     gene            1..1933
                     /gene="Chrna9"
                     /note="cholinergic receptor nicotinic alpha 9 subunit"
                     /db_xref="GeneID:65024"
                     /db_xref="RGD:621534"
     exon            1..150
                     /gene="Chrna9"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1526
                     /gene="Chrna9"
                     /note="neuronal acetylcholine receptor subunit alpha-9;
                     NACHR alpha-9; nicotinic acetylcholine receptor subunit
                     alpha-9; acetylcholine receptor alpha 9 subunit (nAChR);
                     cholinergic receptor, nicotinic, alpha polypeptide 9;
                     cholinergic receptor, nicotinic, alpha 9 (neuronal)"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-9
                     precursor"
                     /protein_id="NP_075219.2"
                     /db_xref="GeneID:65024"
                     /db_xref="RGD:621534"
                     /translation="MNRPHSCLSFCWMYFAASGIRAVETANGKYAQKLFSDLFEDYSS
                     ALRPVEDTDAVLNVTLQVTLSQIKDMDERNQILTAYLWIRQTWHDAYLTWDRDQYDRL
                     DSIRIPSDLVWRPDIVLYNKADDESSEPVNTNVVLRYDGLITWDSPAITKSSCVVDVT
                     YFPFDSQQCNLTFGSWTYNGNQVDIFNALDSGDLSDFIEDVEWEVHGMPAVKNVISYG
                     CCSEPYPDVTFTLLLKRRSSFYIVNLLIPCVLISFLAPLSFYLPAASGEKVSLGVTIL
                     LAMTVFQLMVAEIMPASENVPLIGKYYIATMALITASTALTIMVMNIHFCGAEARPVP
                     HWAKVVILKYMSRILFVYDVGESCLSPRHSQEPEQVTKVYSKLPESNLKTSRNKDLSR
                     KKEVRKLLKNDLGYQGGIPQNTDSYCARYEALTKNIEYIAKCLKDHKATNSKGSEWKK
                     VAKVIDRFFMWIFFAMVFVMTVLIIARAD"
     exon            151..296
                     /gene="Chrna9"
                     /inference="alignment:Splign:2.1.0"
     exon            297..451
                     /gene="Chrna9"
                     /inference="alignment:Splign:2.1.0"
     exon            452..984
                     /gene="Chrna9"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1933
                     /gene="Chrna9"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtggcagtga gggtgttttg tgcccttcac agacagaagt gggagtcctc gctgtctgcc
       61 tgacacattc tacatgttgg gaaaagatga accggcccca ttcctgcctc tccttttgct
      121 ggatgtattt tgctgcttct ggaatcagag ccgtagagac agcaaatggg aaatatgctc
      181 agaaattgtt cagcgatctt tttgaagact actccagtgc tctgcgtcca gtcgaggata
      241 cggacgcggt gctgaatgtt acactgcagg tcacgctctc ccagataaag gacatggacg
      301 agagaaacca gattctgaca gcctatctat ggatccgcca aacctggcac gatgcgtacc
      361 tcacgtggga tcgagaccag tatgataggc tggactccat caggattccc agcgatctgg
      421 tgtggaggcc ggacattgtc ctatacaaca aggctgacga tgagtcttca gagcctgtga
      481 acaccaatgt ggtgctgcga tatgatgggc tcatcacctg ggactcaccg gccatcacca
      541 aaagctcctg tgtggtggat gtcacctact tcccttttga cagccagcag tgcaacctga
      601 cctttggctc ctggacctac aatggaaacc aggtggacat attcaatgcc ctggacagcg
      661 gtgacctctc tgacttcatt gaagatgtgg aatgggaggt ccatggcatg cctgctgtaa
      721 agaacgtcat ctcctatggc tgctgctccg agccttaccc agatgtcacc ttcactctcc
      781 ttctgaagag gaggtcctcc ttctacatcg tcaacctcct catcccttgc gtcctcatat
      841 cgttcctcgc tccgttgagt ttctatctcc cagcagcctc tggggagaag gtctctctgg
      901 gagtgaccat cctattggcc atgactgtgt ttcagctaat ggtggcagag atcatgccag
      961 cctcagaaaa tgtccctctg ataggaaaat actacatagc taccatggcc ttgatcactg
     1021 cctccacagc ccttaccatc atggtgatga atattcactt ctgtggagct gaggcgcggc
     1081 cagtgccaca ctgggccaag gtggtcatcc tgaagtacat gtccaggatc ttgtttgtct
     1141 acgatgtggg tgagagctgc cttagtcccc gccacagcca ggagccagaa caagtcacga
     1201 aggtttatag caaactccca gaatccaacc tgaaaacgtc cagaaacaaa gacctttcca
     1261 gaaagaagga agtaagaaaa ctcttaaaga atgacctggg gtaccagggt gggatccccc
     1321 agaatactga cagttattgt gcacgctatg aagcactgac gaaaaatatc gaatacattg
     1381 ccaagtgcct caaggaccac aaggccacca actccaaggg cagcgagtgg aagaaggtcg
     1441 ccaaagtcat agaccgtttc ttcatgtgga ttttctttgc tatggtgttt gtcatgaccg
     1501 tcttgatcat agcaagagca gattagcagg aaagaggagt gggctggtag gcatttagag
     1561 atttggggaa aacccaataa aatcacctga gatctgcccc agcgtgtgag ttcagctgct
     1621 gttcatacat aatttagggg ataggttgcg tatgctttta ttcctaactt caatcaatat
     1681 cctagttaca tgtcaggtta aatcaagcag gagatgcaag gtttcaaggg taaagggctg
     1741 gaggaagaga gttagaaagg accctttcac aggctcccat gaaggggagt ggtggccttc
     1801 agtttatgta attatctctt tattattgta gacaacaaag cacagtgtat tcctgcttta
     1861 agatttaaag caagaaaaga caaaacaaat tcattctctt agtccttaat aaaacttttt
     1921 tttttaaaca aaa
//
