LOCUS       NM_017078               3007 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus cholinergic receptor nicotinic alpha 5 subunit
            (Chrna5), mRNA.
ACCESSION   NM_017078
VERSION     NM_017078.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Hopkins,R.J., Duan,F., Gamble,G.D., Chiles,C., Cavadino,A.,
            Billings,P., Aberle,D. and Young,R.P.
  TITLE     Chr15q25 genetic variant (rs16969968) independently confers risk of
            lung cancer, COPD and smoking intensity in a prospective study of
            high-risk smokers
  JOURNAL   Thorax 76 (3), 272-280 (2021)
   PUBMED   33419953
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Forget,B., Icick,R., Robert,J., Correia,C., Prevost,M.S.,
            Gielen,M., Corringer,P.J., Bellivier,F., Vorspan,F., Besson,M. and
            Maskos,U.
  TITLE     Alterations in nicotinic receptor alpha5 subunit gene
            differentially impact early and later stages of cocaine addiction:
            a translational study in transgenic rats and patients
  JOURNAL   Prog Neurobiol 197, 101898 (2021)
   PUBMED   32841724
REFERENCE   3  (bases 1 to 3007)
  AUTHORS   Besson,M., Forget,B., Correia,C., Blanco,R. and Maskos,U.
  TITLE     Profound alteration in reward processing due to a human
            polymorphism in CHRNA5: a role in alcohol dependence and feeding
            behavior
  JOURNAL   Neuropsychopharmacology 44 (11), 1906-1916 (2019)
   PUBMED   31288250
  REMARK    GeneRIF: Rats expressing the alpha5SNP consume more alcohol, and
            exhibit increased relapse to alcohol seeking after abstinence.
REFERENCE   4  (bases 1 to 3007)
  AUTHORS   Perez-Morales,R., Gonzalez-Zamora,A., Gonzalez-Delgado,M.F.,
            Calleros Rincon,E.Y., Olivas Calderon,E.H., Martinez-Ramirez,O.C.
            and Rubio,J.
  TITLE     CHRNA3 rs1051730 and CHRNA5 rs16969968 polymorphisms are associated
            with heavy smoking, lung cancer, and chronic obstructive pulmonary
            disease in a mexican population
  JOURNAL   Ann Hum Genet 82 (6), 415-424 (2018)
   PUBMED   29993116
REFERENCE   5  (bases 1 to 3007)
  AUTHORS   Forget,B., Scholze,P., Langa,F., Morel,C., Pons,S., Mondoloni,S.,
            Besson,M., Durand-de Cuttoli,R., Hay,A., Tricoire,L., Lambolez,B.,
            Mourot,A., Faure,P. and Maskos,U.
  TITLE     A Human Polymorphism in CHRNA5 Is Linked to Relapse to Nicotine
            Seeking in Transgenic Rats
  JOURNAL   Curr Biol 28 (20), 3244-3253 (2018)
   PUBMED   30293722
  REMARK    GeneRIF: The alpha5SNP polymorphism is a major risk factor for
            nicotine intake at high doses and for relapse to nicotine seeking
            in rats, a dual effect that reflects the human condition. The
            results also suggest an important role for the IPN in the higher
            relapse to nicotine seeking observed in alpha5SNP rats.
REFERENCE   6  (bases 1 to 3007)
  AUTHORS   Oshikawa,J., Toya,Y., Fujita,T., Egawa,M., Kawabe,J., Umemura,S.
            and Ishikawa,Y.
  TITLE     Nicotinic acetylcholine receptor alpha 7 regulates cAMP signal
            within lipid rafts
  JOURNAL   Am J Physiol Cell Physiol 285 (3), C567-C574 (2003)
   PUBMED   12748066
REFERENCE   7  (bases 1 to 3007)
  AUTHORS   Voitenko,L.P., Voitenko,S.V., Skok,M.V., Purnyn,H.E. and Skok,V.I.
  TITLE     Nicotinic acetylcholine receptor subtypes in rat superior cervical
            ganglion neurons as studied by sequential application of two
            alpha-subunit-specific antibodies
  JOURNAL   Neurosci Lett 303 (1), 37-40 (2001)
   PUBMED   11297818
REFERENCE   8  (bases 1 to 3007)
  AUTHORS   Stitzel,J.A., Blanchette,J.M. and Collins,A.C.
  TITLE     Sensitivity to the seizure-inducing effects of nicotine is
            associated with strain-specific variants of the alpha 5 and alpha 7
            nicotinic receptor subunit genes
  JOURNAL   J Pharmacol Exp Ther 284 (3), 1104-1111 (1998)
   PUBMED   9495872
REFERENCE   9  (bases 1 to 3007)
  AUTHORS   Chini,B., Clementi,F., Hukovic,N. and Sher,E.
  TITLE     Neuronal-type alpha-bungarotoxin receptors and the alpha
            5-nicotinic receptor subunit gene are expressed in neuronal and
            nonneuronal human cell lines
  JOURNAL   Proc Natl Acad Sci U S A 89 (5), 1572-1576 (1992)
   PUBMED   1542648
REFERENCE   10 (bases 1 to 3007)
  AUTHORS   Boulter,J., O'Shea-Greenfield,A., Duvoisin,R.M., Connolly,J.G.,
            Wada,E., Jensen,A., Gardner,P.D., Ballivet,M., Deneris,E.S.,
            McKinnon,D. et al.
  TITLE     Alpha 3, alpha 5, and beta 4: three members of the rat neuronal
            nicotinic acetylcholine receptor-related gene family form a gene
            cluster
  JOURNAL   J Biol Chem 265 (8), 4472-4482 (1990)
   PUBMED   1689727
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Oct 23, 2007 this sequence version replaced NM_017078.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY574255.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840129, SAMN12840131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-163               JAXUCZ010000008.1  64265879-64266041
            164-315             JAXUCZ010000008.1  64284186-64284337
            316-360             JAXUCZ010000008.1  64286938-64286982
            361-470             JAXUCZ010000008.1  64288632-64288741
            471-1299            JAXUCZ010000008.1  64290288-64291116
            1300-3007           JAXUCZ010000008.1  64292526-64294233
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..3007
                     /gene="Chrna5"
                     /note="cholinergic receptor nicotinic alpha 5 subunit"
                     /db_xref="GeneID:25102"
                     /db_xref="RGD:2347"
     exon            1..163
                     /gene="Chrna5"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..1461
                     /gene="Chrna5"
                     /note="acetylcholine receptor alpha 5; cholinergic
                     receptor, nicotinic, alpha polypeptide 5; cholinergic
                     receptor, nicotinic, alpha 5 (neuronal)"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-5"
                     /protein_id="NP_058774.2"
                     /db_xref="GeneID:25102"
                     /db_xref="RGD:2347"
                     /translation="MAVRGSRRRALRLLLMVQLLAGRWRPTGAARGTRGGLPELSSAA
                     KHEDSLFRDLFEDYERWVRPVEHLSDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQE
                     WIDVKLRWNPDDYGGIKIIRVPSDSLWIPDIVLFDNADGRFEGASTKTVVRYNGTVTW
                     TQPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQDVDRTDFFDNGEW
                     EIMSAMGSKGNRTDSCCWYPYITYSFVIKRLPLFYTLFLIIPCIGLSFLTVVVFYLPS
                     NEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFA
                     INIHHRSSSTHNAMAPWVRKIFLHKLPKLLCMRSHADRYFTQREEAESGAGPKSRNTL
                     EAALDCIRYITRHVVKENDVREVVEDWKFIAQVLDRMFLWTFLLVSIIGTLGLFVPVI
                     YKWANIIVPVHIGNTIK"
     exon            164..315
                     /gene="Chrna5"
                     /inference="alignment:Splign:2.1.0"
     exon            316..360
                     /gene="Chrna5"
                     /inference="alignment:Splign:2.1.0"
     exon            361..470
                     /gene="Chrna5"
                     /inference="alignment:Splign:2.1.0"
     exon            471..1299
                     /gene="Chrna5"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..3007
                     /gene="Chrna5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttgctcggcc cctcggggtc cggagttatc cgcgaggcgg ccgtcggcgg gcgggcgatg
       61 gcggtgcggg ggtcgcggcg gcgcgcgctg cgcctgctct tgatggtgca gctgctggca
      121 gggcgctggc ggccgaccgg ggcggcgcgg ggcacgcggg gagggttgcc tgaactatcc
      181 tctgctgcca aacatgaaga cagcttgttt agggatttat ttgaagacta cgaaaggtgg
      241 gttcgccctg tggaacacct gagcgacaag ataaaaatca agtttggcct tgcgatatct
      301 cagttagtgg acgtggatga gaaaaaccag ctgatgacga cgaacgtctg gttgaagcag
      361 gaatggatag atgtgaaatt gagatggaat cctgacgatt atggtgggat aaagattata
      421 cgtgttcctt cggactccct gtggatccca gacatcgttt tgttcgataa tgcagatgga
      481 cgttttgaag gggccagcac gaaaacagtt gtcaggtaca acggcactgt cacgtggacg
      541 caaccagcaa actacaaaag ttcttgcacc atcgacgtta cctttttccc gtttgatctc
      601 caaaattgtt ccatgaaatt cggctcgtgg acatacgatg gatcccaggt tgatataatc
      661 ctagaggacc aagatgtcga cagaacagac tttttcgaca atggagagtg ggaaatcatg
      721 agcgcgatgg ggagcaaggg gaaccggacg gacagctgct gctggtaccc ctacatcacg
      781 tactcctttg tgattaagcg gctgcctctc ttctatacct tgtttcttat tatcccctgc
      841 attgggctct catttctgac tgtggttgtc ttctatctcc cttcaaacga gggtgaaaag
      901 attagcctct gcacctcagt gctcgtctct ctgactgtct tccttttggt aatcgaagag
      961 atcataccgt catcttccaa agtcataccc ctgattgggg agtacttggt gttcaccatg
     1021 attttcgtga ccctatccat tatggtgact gtctttgcca tcaacatcca ccaccgctct
     1081 tcctccacac acaacgctat ggcgccctgg gttcgtaaga tatttctcca caagcttccc
     1141 aagctgctct gcatgagaag tcatgcggat aggtacttca ctcagagaga agaagccgag
     1201 agtggggctg gacctaaatc tcggaacact ttggaagccg cactcgattg cattcgctac
     1261 atcacgaggc acgtcgtgaa agagaacgac gtccgcgagg ttgttgaaga ttggaaattc
     1321 atagcccaag tccttgatcg gatgtttttg tggacgtttc ttctggtgtc aatcattggg
     1381 actttagggc tttttgttcc tgttatttat aaatgggcca atataatagt cccagttcac
     1441 attggaaaca caattaagtg aaaccaagaa attaccctgt ggatttagtg agcagtcatg
     1501 cagctcttag gacatgtatg ctgttatgga aatgtgaagg tagttacaat ttgacatagg
     1561 ctataacaga ttagcaattt ctaacattgg cttaatgttg tccattagaa ctgcagtaat
     1621 aacctcaaat agcaacaaca cattgtctgc ctgcactagt gaaggcctag catctgcatc
     1681 ctggcaaacc ctaccaattt gcaaccaatg atgaaggcca tccttggagt gctggaaaac
     1741 tcaactgtat ttgaagacta tttaaaactc cccccaaatt tagtaggaac atatatatgt
     1801 gtggttttga attttcagaa tgggtctttg ggtcttgtta aattgtctgg gctagcacaa
     1861 aacctcctga gtagctggga ccatgggtgt gctccacttt gccctgttct gtattcacag
     1921 atataaaata catcattatt ttataggagg tagacccatt acttggttta ataataactt
     1981 aatgtcagtt aggtttaatt ataaccttat gtcagctaat gttctattgc tgtgaagaga
     2041 catcatgacc atcaactctt ataaaggaaa acatttcatc agtgctggct tacagttttt
     2101 gaggtttagc caattatcac agtgaaagca tgatagcatc caggtagaca taatgctgga
     2161 tccaggagtt ctctacatct ggatcagcag gcagcaggaa gagagagaga gccactggac
     2221 ctggcttgag catctgaaac ctcaaagccc acctccagtg acacacttcc cccaacaagg
     2281 ccccacctcc taatagtgcc acttgctggt gatcaagcat tcagtctatg ggtctacgag
     2341 agccattcct attcaaacca cacttaatag gatgctattc tttactgaca tttttaataa
     2401 gcgacaaatg gtaactagaa acatcgtagg cccactttac tctttatatg gtaatatgga
     2461 ttggcttttg tattaactag ttttacagcc tatctgaaaa catgtaacag gcaactcctg
     2521 cagacacatt ctttgtaatg actttataat cctgcagtgg catgttccgg taatgacttc
     2581 agtctcctcg tccacactct ccggttagta gagccattta tgtacatcgc atatccctga
     2641 tttcagagca acggtgcagt tgcacaggtt cccacctcaa aatgggatgc catgactctg
     2701 tctggataat tctgtggaaa aaccattctg agctggatac ggtgcctcat acctgtcatg
     2761 tctactttca ggaggcagag gcaggggaat tgctgtgagt tgttggtcag cctgggttac
     2821 agtatgagac cctgtctcag aaaaccaaac aaggggctgg ggatttagct cagtggtaga
     2881 gcgcttacct aggaagcgca aggccctggg ttcggtcccc agctccgaaa aaaagaatca
     2941 aaaaaaaaaa aaaagaaaac caaacaaaaa cttcccctgt gagttgatat aagcacactg
     3001 tcatatc
//
