LOCUS       NM_024354               4406 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus cholinergic receptor nicotinic alpha 4 subunit
            (Chrna4), mRNA.
ACCESSION   NM_024354
VERSION     NM_024354.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Li,D., Huang,C., Liu,Z., Ai,S. and Wang,H.L.
  TITLE     Decreased expression of Chrna4 by METTL3-mediated m6A modification
            participates in BPA-induced spatial memory deficit
  JOURNAL   Environ Res 236 (Pt 1), 116717 (2023)
   PUBMED   37495067
  REMARK    GeneRIF: Decreased expression of Chrna4 by METTL3-mediated m6A
            modification participates in BPA-induced spatial memory deficit.
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Baradaran,R., Anbarkeh,F.R., Delavar,A., Khorasgani,E.M.,
            Rahimian,N., Abbasi,Y. and Jaberi,N.
  TITLE     Hippocampal asymmetry and regional dispersal of nAChRs alpha4 and
            alpha7 subtypes in the adult rat
  JOURNAL   J Chem Neuroanat 116, 101977 (2021)
   PUBMED   34052301
  REMARK    GeneRIF: Hippocampal asymmetry and regional dispersal of nAChRs
            alpha4 and alpha7 subtypes in the adult rat.
REFERENCE   3  (bases 1 to 4406)
  AUTHORS   Borroni,V., Kamerbeek,C., Pediconi,M.F. and Barrantes,F.J.
  TITLE     Lovastatin Differentially Regulates alpha7 and alpha4 Neuronal
            Nicotinic Acetylcholine Receptor Levels in Rat Hippocampal Neurons
  JOURNAL   Molecules 25 (20), 4838 (2020)
   PUBMED   33092257
  REMARK    GeneRIF: Lovastatin Differentially Regulates alpha7 and alpha4
            Neuronal Nicotinic Acetylcholine Receptor Levels in Rat Hippocampal
            Neurons.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4406)
  AUTHORS   Fukuyama,K., Fukuzawa,M., Shiroyama,T. and Okada,M.
  TITLE     Pathogenesis and pathophysiology of autosomal dominant
            sleep-related hypermotor epilepsy with S284L-mutant alpha4 subunit
            of nicotinic ACh receptor
  JOURNAL   Br J Pharmacol 177 (9), 2143-2162 (2020)
   PUBMED   31901135
  REMARK    GeneRIF: Pathogenesis and pathophysiology of autosomal dominant
            sleep-related hypermotor epilepsy with S284L-mutant alpha4 subunit
            of nicotinic ACh receptor.
REFERENCE   5  (bases 1 to 4406)
  AUTHORS   Tarren,J.R., Lester,H.A., Belmer,A. and Bartlett,S.E.
  TITLE     Acute Ethanol Administration Upregulates Synaptic alpha4-Subunit of
            Neuronal Nicotinic Acetylcholine Receptors within the Nucleus
            Accumbens and Amygdala
  JOURNAL   Front Mol Neurosci 10, 338 (2017)
   PUBMED   29114204
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4406)
  AUTHORS   Elliott,K.J., Ellis,S.B., Berckhan,K.J., Urrutia,A.,
            Chavez-Noriega,L.E., Johnson,E.C., Velicelebi,G. and Harpold,M.M.
  TITLE     Comparative structure of human neuronal alpha 2-alpha 7 and beta
            2-beta 4 nicotinic acetylcholine receptor subunits and functional
            expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and
            beta 4 subunits
  JOURNAL   J Mol Neurosci 7 (3), 217-228 (1996)
   PUBMED   8906617
REFERENCE   7  (bases 1 to 4406)
  AUTHORS   Steinlein,O.K., Mulley,J.C., Propping,P., Wallace,R.H.,
            Phillips,H.A., Sutherland,G.R., Scheffer,I.E. and Berkovic,S.F.
  TITLE     A missense mutation in the neuronal nicotinic acetylcholine
            receptor alpha 4 subunit is associated with autosomal dominant
            nocturnal frontal lobe epilepsy
  JOURNAL   Nat Genet 11 (2), 201-203 (1995)
   PUBMED   7550350
REFERENCE   8  (bases 1 to 4406)
  AUTHORS   Connolly,J., Boulter,J. and Heinemann,S.F.
  TITLE     Alpha 4-2 beta 2 and other nicotinic acetylcholine receptor
            subtypes as targets of psychoactive and addictive drugs
  JOURNAL   Br J Pharmacol 105 (3), 657-666 (1992)
   PUBMED   1378342
REFERENCE   9  (bases 1 to 4406)
  AUTHORS   Whiting,P., Esch,F., Shimasaki,S. and Lindstrom,J.
  TITLE     Neuronal nicotinic acetylcholine receptor beta-subunit is coded for
            by the cDNA clone alpha 4
  JOURNAL   FEBS Lett 219 (2), 459-463 (1987)
   PUBMED   3609304
REFERENCE   10 (bases 1 to 4406)
  AUTHORS   Goldman,D., Deneris,E., Luyten,W., Kochhar,A., Patrick,J. and
            Heinemann,S.
  TITLE     Members of a nicotinic acetylcholine receptor gene family are
            expressed in different regions of the mammalian central nervous
            system
  JOURNAL   Cell 48 (6), 965-973 (1987)
   PUBMED   3829125
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000123.1.
            
            [WARNING] On Mar 21, 2024 this sequence was replaced by
            NM_024354.3.
            
            On Nov 24, 2020 this sequence version replaced NM_024354.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M15682.1, AF007212.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-182               JACYVU010000123.1  3754509-3754690     c
            183-334             JACYVU010000123.1  3752941-3753092     c
            335-379             JACYVU010000123.1  3749513-3749557     c
            380-489             JACYVU010000123.1  3749327-3749436     c
            490-1867            JACYVU010000123.1  3743479-3744856     c
            1868-4406           JACYVU010000123.1  3737632-3740170     c
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q43"
     gene            1..4406
                     /gene="Chrna4"
                     /gene_synonym="NARAC"
                     /note="cholinergic receptor nicotinic alpha 4 subunit"
                     /db_xref="GeneID:25590"
                     /db_xref="RGD:2346"
     exon            1..182
                     /gene="Chrna4"
                     /gene_synonym="NARAC"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..1993
                     /gene="Chrna4"
                     /gene_synonym="NARAC"
                     /function="ligand-gated ion channel"
                     /note="neuronal acetylcholine receptor subunit alpha-4;
                     Neuronal nicotinic acetylcholine receptor alpha 4 subunit;
                     cholinergic receptor, nicotinic, alpha polypeptide 4;
                     cholinergic receptor, nicotinic, alpha 4 (neuronal)"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-4
                     precursor"
                     /protein_id="NP_077330.2"
                     /db_xref="GeneID:25590"
                     /db_xref="RGD:2346"
                     /translation="MEIGGPGAPPPLLLLPLLLLLGTGLLPASSHIETRAHAEERLLK
                     RLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRW
                     DPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYK
                     SSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMHSRVDQLDFWESGEWVIVDAVG
                     TYNTRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEK
                     VTLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHH
                     RSPRTHTMPAWVRRVFLDIVPRLLFMKRPSVVKDNCRRLIESMHKMANAPRFWPEPVG
                     EPGILSDICNQGLSPAPTFCNPTDTAVETQPTCRSPPLEVPDLKTSEVEKASPCPSPG
                     SCPPPKSSSGAPMLIKARSLSVQHVPSSQEAAEDGIRCRSRSIQYCVSQDGAASLADS
                     KPTSSPTSLKARPSQLPVSDQASPCKCTCKEPSPVSPVTVLKAGGTKAPPQHLPLSPA
                     LTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPP
                     WLAGMI"
     exon            183..334
                     /gene="Chrna4"
                     /gene_synonym="NARAC"
                     /inference="alignment:Splign:2.1.0"
     exon            335..379
                     /gene="Chrna4"
                     /gene_synonym="NARAC"
                     /inference="alignment:Splign:2.1.0"
     exon            380..489
                     /gene="Chrna4"
                     /gene_synonym="NARAC"
                     /inference="alignment:Splign:2.1.0"
     exon            490..1867
                     /gene="Chrna4"
                     /gene_synonym="NARAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..4406
                     /gene="Chrna4"
                     /gene_synonym="NARAC"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgcggc acggggcatg aagttgggtg cgcgcgggtc tcggagcgga ggcgcggcac
       61 agccgggagc cgccctcgtc tagagcccgc tcggtgcgcc atggagatcg ggggccccgg
      121 ggcgccgccg ccgctgctgc tactgccgct gctgctgctc ctagggaccg gcctcttgcc
      181 tgctagcagc cacatagaga cccgggccca tgcggaggag cggctcctga agagactctt
      241 ctccggctac aacaagtggt ctcggccagt agccaatatc tcagatgtgg tcctcgtccg
      301 ctttggcttg tccattgctc agctcattga cgtggacgag aagaaccaga tgatgacaac
      361 caacgtgtgg gtgaagcagg agtggcacga ctacaagctg cgctgggacc ctggtgacta
      421 cgagaatgtc acctccatcc gcatcccctc tgaactcatc tggaggcctg acatcgtcct
      481 ctacaacaat gcggatggag actttgcagt cacccacctg accaaggccc acctgttcta
      541 tgacggaagg gtgcagtgga cacccccagc catctataag agctcctgca gcatcgacgt
      601 caccttcttc ccctttgacc agcagaactg taccatgaag tttggatcct ggacctacga
      661 caaggccaag attgacttag tgagcatgca tagccgtgtg gaccaactgg acttctggga
      721 aagtggggag tgggtcatcg tggatgctgt gggcacctac aacaccagga agtacgagtg
      781 ctgtgccgag atctatcctg acatcaccta tgccttcatt atccgacggc tgccgctatt
      841 ctacaccatc aacctcatca tcccgtgcct gctcatctcc tgtctcaccg tgctggtctt
      901 ctatctgcct tcagagtgtg gcgagaaggt cacactgtgc atctcggtgc tgctttctct
      961 caccgtcttc ctgctgctca tcaccgagat catcccgtcc acctcgctgg tcatcccgct
     1021 catcggcgag tacctcctct tcaccatgat cttcgtcacc ctctccatcg tcatcacggt
     1081 cttcgtgctc aatgtgcacc accgctcgcc acgcacacac acgatgcccg cctgggtgcg
     1141 tagagtcttc ctggacatcg tgcctcgcct cctcttcatg aagcgcccct ctgtggtcaa
     1201 agacaactgc cggagactta ttgagtccat gcacaagatg gccaacgccc cccgcttctg
     1261 gccagagcct gtgggcgagc ccggcatctt gagtgacatc tgcaaccaag gtctgtcacc
     1321 tgccccaact ttctgcaacc ccacggacac agcagtcgag acccagccta cgtgcaggtc
     1381 accccccctt gaggtccctg acttgaagac atcagaggtt gagaaggcca gtccctgtcc
     1441 atcgcctggc tcctgtcctc cacccaagag cagcagtggg gctccaatgc tcatcaaagc
     1501 caggtccctg agtgtccagc atgtgcccag ctcccaagaa gcagcagaag atggcatccg
     1561 ctgccggtct cggagtatcc agtactgtgt ttcccaagat ggagctgcct ccctggctga
     1621 cagcaagccc accagctccc cgacctccct gaaggcccgt ccatcccagc ttcccgtgtc
     1681 agaccaggcc tctccatgca aatgcacatg caaggaacca tctcctgtgt ccccagtcac
     1741 tgtgctcaag gcgggaggca ccaaagcacc tccccaacac ctgcccctgt caccagccct
     1801 gacacgggca gtagaaggcg tccagtacat tgcagaccac ctcaaggcag aagacactga
     1861 cttctcggtg aaggaggact ggaaatacgt ggccatggtc attgaccgaa tcttcctctg
     1921 gatgttcatc attgtctgcc ttctgggcac tgtgggactc ttcctgcctc cctggctggc
     1981 tggtatgatc tagggacgtg gtggtgccca gctcccacgt ctctgtaggg ccatacgact
     2041 cgtcagtcac ccacatcttc caaaccggct gaccatgaga cacctaggag agagatgatg
     2101 cttcttggga gctggaagtt ggccctggtt ctagtcagac tatgggcctg gttggagaga
     2161 aatgagggct gatacagttg caggccgagt ccccattaaa gtttctccag agcaagtggc
     2221 agtcactccc tgacttacag acagcacaca cccatctgtg tcacagagaa tgatcccgag
     2281 ttgatctcag ttgtcctttg aggccatgaa aaattcatcc accttcagga accagagcct
     2341 ctcatgctgt gggatcaata agaccaggaa tctcccactg tgactctgct ggccacaccc
     2401 tctccctccc ccaagaagtg gtccctcatc cctccaggtt gggctactct ctaactcaga
     2461 ggtgtcaggt gtgagcccgg gcaggttttt attttgttta ggttgggaca aattggtgtt
     2521 taggttggga caaattggtg agaaacagaa cagtgagcta tatggatggg tcccactcac
     2581 aggagaacga gttcaggtcc tcacaggtag actgaagcac cacaggaatt ctgcttccac
     2641 ctcagggtca cagggctctg tggtgagcat ttcctagggt ccagagaccc caggagggca
     2701 agagcattag gagtctcagg gttgtgatgg agccaaatca tgtccagggc ctggacatcc
     2761 cctcatggat cttccttgat atccctgtat gcttctgcct ctctgggatt agaaggctga
     2821 aggtaagact tctcatcaca gtcctgtggt atggccacag ttcctcctca atacgtctct
     2881 ctagaccagc aggagtgatg ggaagcccct tcgatgttgt ggaatagggt gagctgccaa
     2941 gagactctaa gaaaacaggc cctcgactca ttgtttgagg ggcatcgacc aagggtcagg
     3001 ggtgcctcac ccagtctgcc ttgcacttag gttgtaggga agacctgagg ggctcctctt
     3061 ccccttccca agatggcacc aggcaacctg gagaatcccc agtggtggga gaaagatcag
     3121 tacccagctc tatgagattc tactgggtga agggcaggac atgggagaca cccaagaaat
     3181 cgccccatgt catggtccca gttgcccctc tgccccacca gccagatggg tttcccagag
     3241 ctgagcccta caccctcagc caagttgttc taagccctaa agctgaggtc ccagtttgga
     3301 tagtcctggg gactgcagaa atgaaagaac atatgagcac accaagcctc cagtaggtat
     3361 agctgttgcc ctcatggtat gacttgccca tcccagctgc atgaggatgt caggaaggag
     3421 gtaagccaga ggaccagcac tgtccctacc tgactatctg cagggaaatc cacctttaaa
     3481 cacagagctg ctgggcatcc agggtgctgg tgggaaagga accccatgct ggaagcccca
     3541 gaacattcct gtgtacagga aggggatgca gaggcctggg ccctgaatcc tggatgttcc
     3601 aggtcttccc tagagtccta aaggcatcaa ggatcccatc cgccatgttt caacctgccc
     3661 tccactgact ggctaggtca ccacccccta tactggaact ctggggatgc tgcaagataa
     3721 ccccgctggg gaaattcatg ccagaacctg ggagtcaagg ggaacacaag ccccagtgat
     3781 gaaggcggca gataacacct gacagttgca tgtgtctcca accacaagaa acgcagacaa
     3841 cagtagagac cagggaaagg ccacagtgac acggaacaga actgccgtgc tattctcttc
     3901 agggccagga aaacggaatg gctggggtgc tcctgacagg cacacaccca gaagccagaa
     3961 aaggaaaata aagcaccgtg ttctccccat tcccactcag ccggcctttt gtctgcccac
     4021 ttccagtgtt gacatacggt caaaatgaag tacaattagg gcgaatgctt gacccaaggc
     4081 ctttgaacct gtgagcgttt taaggacaga gactaggtag ccccggtctc tctgatatgt
     4141 agacattatc tcccaaagcc cccagtcctc ccattctacc ccgtcccatg gacagcagca
     4201 actcaaatgt gagtcttgaa ttgcagttca gctcgtcatc atgtctggga tcccatatcg
     4261 gactctgcga tgtttggctt ctttcgcttg tgtggccaag gctcatctgc agtgttgtgt
     4321 gcagtagact cattgctgtt gtgtgcttgt tggttcttag ttgttgtttc tgtctgaata
     4381 aaccgagtgg tggtgtctcc ccatca
//
