LOCUS       NM_022639               2172 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus cholinergic receptor nicotinic alpha 10 subunit
            (Chrna10), mRNA.
ACCESSION   NM_022639
VERSION     NM_022639.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Gallino,S.L., Aguero,L., Boffi,J.C., Schottlender,G.,
            Buonfiglio,P., Dalamon,V., Marcovich,I., Carpaneto,A., Craig,P.O.,
            Plazas,P.V. and Elgoyhen,A.B.
  TITLE     Key role of the TM2-TM3 loop in calcium potentiation of the
            alpha9alpha10 nicotinic acetylcholine receptor
  JOURNAL   Cell Mol Life Sci 81 (1), 337 (2024)
   PUBMED   39120784
  REMARK    GeneRIF: Key role of the TM2-TM3 loop in calcium potentiation of
            the alpha9alpha10 nicotinic acetylcholine receptor.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Boffi,J.C., Marcovich,I., Gill-Thind,J.K., Corradi,J., Collins,T.,
            Lipovsek,M.M., Moglie,M., Plazas,P.V., Craig,P.O., Millar,N.S.,
            Bouzat,C. and Elgoyhen,A.B.
  TITLE     Differential Contribution of Subunit Interfaces to alpha9alpha10
            Nicotinic Acetylcholine Receptor Function
  JOURNAL   Mol Pharmacol 91 (3), 250-262 (2017)
   PUBMED   28069778
  REMARK    GeneRIF: the contribution of subunit interface domains to
            alpha9alpha10 receptors by a combination of electrophysiological
            and radioligand binding studies, is reported.
            Erratum:[Mol Pharmacol. 2017 May;91(5):437. doi:
            10.1124/mol.111.107482err.. PMID: 28325810]
REFERENCE   3  (bases 1 to 2172)
  AUTHORS   Lipovsek,M., Fierro,A., Perez,E.G., Boffi,J.C., Millar,N.S.,
            Fuchs,P.A., Katz,E. and Elgoyhen,A.B.
  TITLE     Tracking the molecular evolution of calcium permeability in a
            nicotinic acetylcholine receptor
  JOURNAL   Mol Biol Evol 31 (12), 3250-3265 (2014)
   PUBMED   25193338
REFERENCE   4  (bases 1 to 2172)
  AUTHORS   Azam,L. and McIntosh,J.M.
  TITLE     Molecular basis for the differential sensitivity of rat and human
            alpha9alpha10 nAChRs to alpha-conotoxin RgIA
  JOURNAL   J Neurochem 122 (6), 1137-1144 (2012)
   PUBMED   22774872
  REMARK    GeneRIF: A two order of magnitude species difference in potency of
            alpha-conotoxin RgIA for rat versus human alpha9alpha10 nAChR.
REFERENCE   5  (bases 1 to 2172)
  AUTHORS   Lipovsek,M., Im,G.J., Franchini,L.F., Pisciottano,F., Katz,E.,
            Fuchs,P.A. and Elgoyhen,A.B.
  TITLE     Phylogenetic differences in calcium permeability of the auditory
            hair cell cholinergic nicotinic receptor
  JOURNAL   Proc Natl Acad Sci U S A 109 (11), 4308-4313 (2012)
   PUBMED   22371598
  REMARK    GeneRIF: The properties of rat and chicken recombinant and native
            alpha9alpha10 receptors, were compared.
REFERENCE   6  (bases 1 to 2172)
  AUTHORS   Baker,E.R., Zwart,R., Sher,E. and Millar,N.S.
  TITLE     Pharmacological properties of alpha 9 alpha 10 nicotinic
            acetylcholine receptors revealed by heterologous expression of
            subunit chimeras
  JOURNAL   Mol Pharmacol 65 (2), 453-460 (2004)
   PUBMED   14742688
REFERENCE   7  (bases 1 to 2172)
  AUTHORS   Morley,B.J. and Simmons,D.D.
  TITLE     Developmental mRNA expression of the alpha10 nicotinic
            acetylcholine receptor subunit in the rat cochlea
  JOURNAL   Brain Res Dev Brain Res 139 (1), 87-96 (2002)
   PUBMED   12414097
  REMARK    GeneRIF: developmental mRNA expression of the alpha10 nicotinic
            acetylcholine receptor subunit in the rat cochlea
REFERENCE   8  (bases 1 to 2172)
  AUTHORS   Weisstaub,N., Vetter,D.E., Elgoyhen,A.B. and Katz,E.
  TITLE     The alpha9alpha10 nicotinic acetylcholine receptor is permeable to
            and is modulated by divalent cations
  JOURNAL   Hear Res 167 (1-2), 122-135 (2002)
   PUBMED   12117536
  REMARK    GeneRIF: permeability of the recombinant alpha 9 alpha 10 nicotinic
            acetylcholine receptor to Ca(2+), Ba(2+) and Mg(2+) and its
            modulation by these divalent cations
REFERENCE   9  (bases 1 to 2172)
  AUTHORS   Lips,K.S., Pfeil,U. and Kummer,W.
  TITLE     Coexpression of alpha 9 and alpha 10 nicotinic acetylcholine
            receptors in rat dorsal root ganglion neurons
  JOURNAL   Neuroscience 115 (1), 1-5 (2002)
   PUBMED   12401316
  REMARK    GeneRIF: Dorsal root ganglion neurons coexpress alpha 10 nicotinic
            acetylcholine receptor subunit with the alpha 9 subunit, thereby
            providing the first example of neuronal expression of this receptor
            subunit pair.
REFERENCE   10 (bases 1 to 2172)
  AUTHORS   Elgoyhen,A.B., Vetter,D.E., Katz,E., Rothlin,C.V., Heinemann,S.F.
            and Boulter,J.
  TITLE     alpha10: a determinant of nicotinic cholinergic receptor function
            in mammalian vestibular and cochlear mechanosensory hair cells
  JOURNAL   Proc Natl Acad Sci U S A 98 (6), 3501-3506 (2001)
   PUBMED   11248107
  REMARK    GeneRIF: original cloning and developmental analysis of alpha10
            expression
            GeneRIF: Co-expression of the alpha10 subunit converts the normally
            weak acetylcholine-mediated activity of alpha9 to a more robustly
            active channel with identical physiological characteristics of the
            native mammalian outer hair cell cholinergic receptor.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF196344.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF196344.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00155310,
                                           SAMEA5760383 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..2172
                     /gene="Chrna10"
                     /note="cholinergic receptor nicotinic alpha 10 subunit"
                     /db_xref="GeneID:64574"
                     /db_xref="RGD:620142"
     exon            1..124
                     /gene="Chrna10"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1407
                     /gene="Chrna10"
                     /note="neuronal acetylcholine receptor subunit alpha-10;
                     NACHR alpha-10; nicotinic acetylcholine receptor subunit
                     alpha-10; neuronal nicotinic acetylcholine receptor
                     subunit; cholinergic receptor, nicotinic, alpha
                     polypeptide 10; cholinergic receptor, nicotinic, alpha 10
                     (neuronal)"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-10
                     precursor"
                     /protein_id="NP_072161.1"
                     /db_xref="GeneID:64574"
                     /db_xref="RGD:620142"
                     /translation="MGTRSHYLDLGFLLLLFLPAECLGAEGRLAHKLFRDLFANYTSA
                     LRPVADTDQTLNVTLEVTLSQIIDMDERNQVLTLYLWIRQEWTDAYLHWDPKAYGDLD
                     AIRIPSRLVWRPDIVLYNKADTQPPASASTNVVVRHDGAVRWDAPAITRSSCRVDVSA
                     FPFDAQRCGLTFGSWTHGGHQLDVRPRGTSASLADFVENVEWRVLGMPARRRVLTYGC
                     CSEPYPDVTFTLLLRRRAAAYVCNLLLPCVFISLLAPLAFHLPADSGEKVSLGVTVLL
                     ALTVFQLILAESMPPAESVPLIGKYYMATMTMVTFSTALTILIMNLHYCGPNAHPVPA
                     WARVLLLGHLAKGLCVRERGEPCGQSKPLESAPSLQPPPASPAGPCHEPRCLCHQEAL
                     LHHIASIASTFRSHRAAQRRHEDWKRLARVMDRFFLGIFFCMALVMSLIVLVQAL"
     exon            125..270
                     /gene="Chrna10"
                     /inference="alignment:Splign:2.1.0"
     exon            271..425
                     /gene="Chrna10"
                     /inference="alignment:Splign:2.1.0"
     exon            426..958
                     /gene="Chrna10"
                     /inference="alignment:Splign:2.1.0"
     exon            959..2170
                     /gene="Chrna10"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctggacct gttgtcctac atcgctcctg aagacttgcc caccctctgg ctgtggtagg
       61 gccatgggga caaggagcca ctacctggac ctggggtttc tgctgctgct gtttctccct
      121 gcagagtgcc tgggagctga ggggagactg gctcacaagc tgtttcgtga cctgtttgcc
      181 aactatacaa gtgctctgag accagtggca gatacagacc agactctaaa tgtgaccctg
      241 gaggtgacat tgtctcagat cattgatatg gatgaacgga accaggtgct gaccttgtac
      301 ctgtggatcc ggcaagagtg gacagatgcc tacctacact gggaccccaa agcctatggt
      361 gacctggacg caatccgaat tcccagccgt ctagtgtggc gaccagacat cgtactttac
      421 aacaaggcgg acacgcagcc acctgcctca gccagcacca acgtggttgt gcggcacgac
      481 ggcgctgtgc gctgggacgc accggccatc acacgcagct cgtgccgtgt ggacgtgtct
      541 gccttccctt ttgacgcgca gcgctgcggc ctgaccttcg gctcatggac gcacggtggg
      601 caccagctgg atgtgcgacc tcggggcact tccgccagtc tggccgactt cgtggagaac
      661 gttgaatggc gggtgctggg catgccagca cgcaggcggg tcctcaccta tggctgctgc
      721 tctgagccct acccagatgt gacctttact ctgttgctgc gccgccgcgc tgcagcctac
      781 gtgtgcaacc tcctgctgcc ctgtgtgttc atctccctgc tggcgcctct ggccttccac
      841 ctgcctgctg actctgggga gaaggtgtct ctgggcgtca ccgtgctcct ggcgctcacc
      901 gtcttccagc tgatcctggc cgagagcatg ccacctgcag agagtgtgcc actcatcgga
      961 aagtactata tggccactat gaccatggtc acattctcca cagcacttac catcctcatc
     1021 atgaatctgc actactgtgg ccctaatgca catccagtgc ccgcctgggc tcgggttctc
     1081 ctgctgggac acctagccaa aggcctgtgt gtgcgggaac gaggggagcc ctgtgggcag
     1141 tccaagccac tagagtcagc ccccagcctc cagcctccac cagcttcccc agcgggccct
     1201 tgtcatgagc cacggtgtct atgccaccag gaagcccttc tacatcatat agcttccatt
     1261 gctagtacct tccgcagcca ccgggctgcc cagcgccgcc acgaagattg gaagcgtctg
     1321 gctagagtaa tggaccgctt tttcctaggc atcttcttct gcatggctct ggtcatgagc
     1381 ctcattgtac tggtgcaagc cctgtaaggg ccaggaactg gatttcaggg agctacggtc
     1441 acatcaacac caccaggcag gaatggcaaa gcccaggtgg ttgttggtac tcagatgtac
     1501 tagccagtct ctccccaaag tgctgctaac agtcagagac acctcttcag gaccagtgct
     1561 tctgacctca caacccacaa acagcctttg cgatctacat gccctcatgg agggggatct
     1621 aaagcccacc tctaattcca acatgattaa ggtcaaagta caaacttgga ggataagact
     1681 caatgccaga tgagcacggt aattccagca catggaggct gagacaggag gattacctta
     1741 agttctgtag cctgaggcat aaaaaatttt tttgaatagt caccccattg tctatacttg
     1801 gaatcatagc tattggctgg gggaatccag gttatttctg tctattgaga cagagtctca
     1861 ctatgtagtc cttgctatcc tggaactcgc aatgaagccc aggctggctt tgaactcaca
     1921 tagatccaca tgcctctgcc tctccaaaca caggtgccac tatgcctggc tagagtgtag
     1981 gttactaatt aaagggacaa agccacactt tgcttgtcac ccttagcagt tgctgttaca
     2041 gattctcacc tggcttgttt ctgcttaggc tctcccggaa gggagttcac ggtctagttt
     2101 ccagaacaga aggctgtgca gacatttgac ccagaacttc aatccataat aaatgagagt
     2161 attcccagca aa
//
