LOCUS       NM_017362               2351 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Rattus norvegicus cholinergic receptor, muscarinic 5 (Chrm5), mRNA.
ACCESSION   NM_017362
VERSION     NM_017362.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Kauschke,V., Lips,K.S., Heiss,C. and Schnettler,R.
  TITLE     Expression of muscarinic acetylcholine receptors M3 and M5 in
            osteoporosis
  JOURNAL   Med Sci Monit 20, 869-874 (2014)
   PUBMED   24866457
  REMARK    GeneRIF: The relative mRNA expression of M3 and M5 mAChR in bones
            of a rat osteoporosis model, was investigated.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Garzon,M. and Pickel,V.M.
  TITLE     Somatodendritic targeting of M5 muscarinic receptor in the rat
            ventral tegmental area: implications for mesolimbic dopamine
            transmission
  JOURNAL   J Comp Neurol 521 (13), 2927-2946 (2013)
   PUBMED   23504804
  REMARK    GeneRIF: This study demonistrted that Somatodendritic targeting of
            M5 muscarinic receptor in the rat ventral tegmental area:
            implications for mesolimbic dopamine transmission.
REFERENCE   3  (bases 1 to 2351)
  AUTHORS   Thiele,A.
  TITLE     Muscarinic signaling in the brain
  JOURNAL   Annu Rev Neurosci 36, 271-294 (2013)
   PUBMED   23841840
  REMARK    Review article
REFERENCE   4  (bases 1 to 2351)
  AUTHORS   Harada,K., Matsuoka,H., Sata,T., Warashina,A. and Inoue,M.
  TITLE     Identification and role of muscarinic receptor subtypes expressed
            in rat adrenal medullary cells
  JOURNAL   J Pharmacol Sci 117 (4), 253-264 (2011)
   PUBMED   22095037
  REMARK    GeneRIF: M5 receptor is the major muscarinic receptor subtype in
            rat adrenal medullary cells and may be involved in neuronal
            transmission under conditions where acetylcholine spills over the
            synapse
REFERENCE   5  (bases 1 to 2351)
  AUTHORS   Zou,Y. and Kong,W.
  TITLE     [Expression change of Muscarinic receptor subunits in rat flocculus
            following unilateral labyrinthectomy]
  JOURNAL   Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 22 (19), 896-898
            (2008)
   PUBMED   19160866
  REMARK    GeneRIF: Expression of muscarinic receptor M1, M3, M5 subunits
            decreased in the flocculus after unilateral labyrinthectomy.
REFERENCE   6  (bases 1 to 2351)
  AUTHORS   Tseng,J., Erbe,C.B., Kwitek,A.E., Jacob,H.J., Popper,P. and
            Wackym,P.A.
  TITLE     Radiation hybrid mapping of five muscarinic acetylcholine receptor
            subtype genes in Rattus norvegicus
  JOURNAL   Hear Res 174 (1-2), 86-92 (2002)
   PUBMED   12433399
  REMARK    GeneRIF: The m1-m5 muscarinic subtypes mapped to the following
            chromosomes: Chrm1, chromosome 1; Chrm2, chromosome 4; Chrm3,
            chromosome 17; Chrm4, chromosome 3; and Chrm5, chromosome 3.
REFERENCE   7  (bases 1 to 2351)
  AUTHORS   Forster,G.L., Yeomans,J.S., Takeuchi,J. and Blaha,C.D.
  TITLE     M5 muscarinic receptors are required for prolonged accumbal
            dopamine release after electrical stimulation of the pons in mice
  JOURNAL   J Neurosci 22 (1), RC190 (2002)
   PUBMED   11756520
REFERENCE   8  (bases 1 to 2351)
  AUTHORS   Kurtenbach,E., Curtis,C.A., Pedder,E.K., Aitken,A., Harris,A.C. and
            Hulme,E.C.
  TITLE     Muscarinic acetylcholine receptors. Peptide sequencing identifies
            residues involved in antagonist binding and disulfide bond
            formation
  JOURNAL   J Biol Chem 265 (23), 13702-13708 (1990)
   PUBMED   2380182
REFERENCE   9  (bases 1 to 2351)
  AUTHORS   Liao,C.F., Themmen,A.P., Joho,R., Barberis,C., Birnbaumer,M. and
            Birnbaumer,L.
  TITLE     Molecular cloning and expression of a fifth muscarinic
            acetylcholine receptor
  JOURNAL   J Biol Chem 264 (13), 7328-7337 (1989)
   PUBMED   2540186
REFERENCE   10 (bases 1 to 2351)
  AUTHORS   Bonner,T.I., Young,A.C., Brann,M.R. and Buckley,N.J.
  TITLE     Cloning and expression of the human and rat m5 muscarinic
            acetylcholine receptor genes
  JOURNAL   Neuron 1 (5), 403-410 (1988)
   PUBMED   3272174
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Nov 25, 2020 this sequence version replaced NM_017362.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M22926.1, SRR26360176.774645.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMN12840106
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-509               JAXUCZ010000003.1  119791122-119791630 c
            510-2351            JAXUCZ010000003.1  119739229-119741070 c
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q35"
     gene            1..2351
                     /gene="Chrm5"
                     /note="cholinergic receptor, muscarinic 5"
                     /db_xref="GeneID:53949"
                     /db_xref="RGD:620027"
     exon            1..509
                     /gene="Chrm5"
                     /inference="alignment:Splign:2.1.0"
     exon            510..2351
                     /gene="Chrm5"
                     /inference="alignment:Splign:2.1.0"
     CDS             592..2187
                     /gene="Chrm5"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M5"
                     /protein_id="NP_059058.1"
                     /db_xref="GeneID:53949"
                     /db_xref="RGD:620027"
                     /translation="MEGESYNESTVNGTPVNHQALERHGLWEVITIAVVTAVVSLMTI
                     VGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTTYILMGRWVLGSLAC
                     DLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLVSFIL
                     WAPAILCWQYLVGKRTVPPDECQIQFLSEPTITFGTAIAAFYIPVSVMTILYCRIYRE
                     TEKRTKDLADLQGSDSVAEAKKREPAQRTLLRSFFSCPRPSLAQRERNQASWSSSRRS
                     TSTTGKTTQATDLSADWEKAEQVTTCSSYPSSEDEAKPTTDPVFQMVYKSEAKESPGK
                     ESNTQETKETVVNTRTENSDYDTPKYFLSPAAAHRLKSQKCVAYKFRLVVKADGTQET
                     NNGCRKVKIMPCSFPVSKDPSTKGPDPNLSHQMTKRKRMVLVKERKAAQTLSAILLAF
                     IITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTINPICYALCNRTFRKTFKLL
                     LLCRWKKKKVEEKLYWQGNSKLP"
ORIGIN      
        1 gaaagaaagg aaatgcttaa cctctgcaga caggagactg aaggacggaa agttgataag
       61 ggaaaagcca agggaagcag aaggtgttaa agaataccca cgcttctgga acacacaaca
      121 gtgttacagc agcgtgatct ggagatgctg gagactctaa attacaggtg agcagaactt
      181 ttccagaggc tgaaacatct acaagagcaa tcaggatttc tcactgctga gataaccaat
      241 gaatttttaa taagttctga gctctgtggt cagatcaatg gctgttatcc gataaagaca
      301 aattttaccc tttgatttcc ataatcctgt ccgaatggaa tcttcacctt gctgctacct
      361 gtgctaacta atgccacggg attaccgtgc ctcagagatg ctgcaaaaaa taagacacat
      421 gggtgaaaga gatcaccaca gcaaagtcga tgaggcattt ccagtgagag tttacccttc
      481 ctcgctgagc ttctaccaac tcctgccaga tattggccaa gaagagggga ggccaaacat
      541 agagcagctc caaaccttgg tctaaatctg aatgtaattt cctgagccag gatggaaggg
      601 gagtcttaca atgaaagcac tgtcaacggc accccggtaa atcaccaggc tttggaacgc
      661 catggactgt gggaagtcat tactattgca gttgtgactg cggtggtcag cctgatgacc
      721 attgtcggca atgtcttggt catgatctcc ttcaaagtca acagtcagct caagacagtt
      781 aacaactact acctgctcag cttggcctgt gcagacctca tcattggcat cttctccatg
      841 aacctctaca cgacctacat cctcatggga cgctgggttc tcgggagtct ggcttgtgac
      901 ctttggctgg cactcgacta tgtagccagc aatgcttctg tcatgaacct tctggtgatt
      961 agttttgatc gttacttttc catcacaaga ccactgacgt accgggccaa gcgtacccca
     1021 aagagggctg gcatcatgat tggcttagca tggctggtct ccttcatcct ctgggcgcca
     1081 gccatcctct gctggcagta cttggtcggg aagcggacag taccacctga tgagtgccag
     1141 atccagttcc tctctgaacc caccatcact tttgggactg ccattgctgc cttctacatc
     1201 cctgtctccg tcatgaccat actctactgc cggatctacc gggagacaga gaagcgaacc
     1261 aaggacctgg ctgacctcca aggttctgat tctgtggcag aagccaagaa gagagagcca
     1321 gctcaaagga ccctgctcag atctttcttt agctgcccta gacccagcct ggcccagaga
     1381 gaaaggaatc aggcctcctg gtcatcctcc cgtagaagca cctcaacaac aggaaagaca
     1441 acccaggcca ctgacctaag tgctgactgg gaaaaggctg agcaggttac tacctgtagc
     1501 agctacccct cttcagagga tgaagccaag cccaccactg accctgtctt tcaaatggtc
     1561 tacaagagtg aggccaagga aagcccgggg aaggaatcca atacccaaga gaccaaggaa
     1621 acagttgtga acacccggac tgaaaacagt gactatgaca ctcccaaata ctttctgtct
     1681 ccagctgctg ctcacagact caagagtcag aagtgtgttg cctataagtt ccgattggtg
     1741 gtaaaagccg atgggaccca ggagactaac aatggctgtc gaaaggtgaa aatcatgccc
     1801 tgttccttcc cagtgtccaa agacccttca acaaaaggtc cggatcccaa cctcagtcat
     1861 caaatgacca aacgaaagag aatggttctg gtcaaagaga ggaaagcggc tcagaccttg
     1921 agtgccattc tcctggcctt catcatcaca tggacccctt ataacatcat ggtcctggtt
     1981 tccaccttct gtgacaagtg tgtccccgtc accctgtggc acttgggtta ctggctgtgc
     2041 tatgtcaaca gcaccatcaa ccccatctgc tatgctctct gcaacagaac tttcaggaaa
     2101 acctttaagc tgcttcttct ctgccggtgg aaaaagaaaa aagtagaaga gaaattgtat
     2161 tggcaaggaa acagcaagct accctgaaac cttagcacct cccctggaaa gaataatgat
     2221 caccgtctgg tttgcttgtt taaagaacgt catctgctgt tctgagtcct tgaaggcttg
     2281 tcaaggtgca aaatcttctg tcaaatgaag agtcgtcaca catgcacctg ccatgtgcgg
     2341 tgagtcttga c
//
