LOCUS       NM_031547               2768 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus cholinergic receptor, muscarinic 4 (Chrm4), mRNA.
ACCESSION   NM_031547 XM_001078223 XM_345403
VERSION     NM_031547.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Brugnoli,A., Pisano,C.A. and Morari,M.
  TITLE     Striatal and nigral muscarinic type 1 and type 4 receptors modulate
            levodopa-induced dyskinesia and striato-nigral pathway activation
            in 6-hydroxydopamine hemilesioned rats
  JOURNAL   Neurobiol Dis 144, 105044 (2020)
   PUBMED   32798726
  REMARK    GeneRIF: Striatal and nigral muscarinic type 1 and type 4 receptors
            modulate levodopa-induced dyskinesia and striato-nigral pathway
            activation in 6-hydroxydopamine hemilesioned rats.
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Lima,J.D., Sobrinho,C.R., Santos,L.K., Takakura,A.C. and
            Moreira,T.S.
  TITLE     M4-muscarinic acetylcholine receptor into the pedunculopontine
            tegmental nucleus mediates respiratory modulation of conscious rats
  JOURNAL   Respir Physiol Neurobiol 269, 103254 (2019)
   PUBMED   31325565
  REMARK    GeneRIF: Our data suggest that the changes in respiratory frequency
            and tidal volume produced by muscarinic cholinergic stimulation of
            the pedunculopontine tegmental nucleus is presumably mediated
            through a Gi-coupled M4 muscarinic receptors.
REFERENCE   3  (bases 1 to 2768)
  AUTHORS   He,N., Mao,L.M., Sturich,A.W., Jin,D.Z. and Wang,J.Q.
  TITLE     Inhibition of basal and amphetamine-stimulated extracellular
            signal-regulated kinase (ERK) phosphorylation in the rat forebrain
            by muscarinic acetylcholine M4 receptors
  JOURNAL   Brain Res 1688, 103-112 (2018)
   PUBMED   29577888
  REMARK    GeneRIF: Study showed that the muscarinic M4 receptor-preferring
            antagonist tropicamide consistently increased ERK phosphorylation
            in the striatum and medial prefrontal cortex.
REFERENCE   4  (bases 1 to 2768)
  AUTHORS   Thiele,A.
  TITLE     Muscarinic signaling in the brain
  JOURNAL   Annu Rev Neurosci 36, 271-294 (2013)
   PUBMED   23841840
  REMARK    Review article
REFERENCE   5  (bases 1 to 2768)
  AUTHORS   Kovalenko,N.Y., Matsievskii,D.D. and Reshetnyak,V.K.
  TITLE     Role of various subtypes of muscarinic cholinergic receptors in the
            development of posthemorrhagic abnormalities in systemic and portal
            circulation in rats
  JOURNAL   Bull Exp Biol Med 152 (3), 293-297 (2012)
   PUBMED   22803069
  REMARK    GeneRIF: M1 and M4 muscarinic receptors are involved in
            shock-limiting and shock-activating processes, respectively, on the
            course of posthemorrhagic abnormalities in systemic and hepatic
            portal circulation.
REFERENCE   6  (bases 1 to 2768)
  AUTHORS   Szpirer,C., Szpirer,J., Van Vooren,P., Tissir,F., Simon,J.S.,
            Koike,G., Jacob,H.J., Lander,E.S., Helou,K., Klinga-Levan,K. and
            Levan,G.
  TITLE     Gene-based anchoring of the rat genetic linkage and cytogenetic
            maps: new regional localizations, orientation of the linkage
            groups, and insights into mammalian chromosome evolution
  JOURNAL   Mamm Genome 9 (9), 721-734 (1998)
   PUBMED   9716657
REFERENCE   7  (bases 1 to 2768)
  AUTHORS   Mieda,M., Haga,T. and Saffen,D.W.
  TITLE     Promoter region of the rat m4 muscarinic acetylcholine receptor
            gene contains a cell type-specific silencer element
  JOURNAL   J Biol Chem 271 (9), 5177-5182 (1996)
   PUBMED   8617799
REFERENCE   8  (bases 1 to 2768)
  AUTHORS   Hersch,S.M., Gutekunst,C.A., Rees,H.D., Heilman,C.J. and Levey,A.I.
  TITLE     Distribution of m1-m4 muscarinic receptor proteins in the rat
            striatum: light and electron microscopic immunocytochemistry using
            subtype-specific antibodies
  JOURNAL   J Neurosci 14 (5 Pt 2), 3351-3363 (1994)
   PUBMED   8182478
REFERENCE   9  (bases 1 to 2768)
  AUTHORS   van Koppen,C.J., Lenz,W. and Nathanson,N.M.
  TITLE     Isolation, sequence and functional expression of the mouse m4
            muscarinic acetylcholine receptor gene
  JOURNAL   Biochim Biophys Acta 1173 (3), 342-344 (1993)
   PUBMED   7916637
REFERENCE   10 (bases 1 to 2768)
  AUTHORS   Bonner,T.I., Buckley,N.J., Young,A.C. and Brann,M.R.
  TITLE     Identification of a family of muscarinic acetylcholine receptor
            genes
  JOURNAL   Science 237 (4814), 527-532 (1987)
   PUBMED   3037705
  REMARK    Erratum:[Science 1987 Sep 25;237(4822):237]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV213670.1, M16409.1 and CR472483.1.
            
            On or before Oct 13, 2007 this sequence version replaced
            XM_345403.3, XM_001078223.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DV213670.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132264
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-505               DV213670.1         1-505
            506-2529            M16409.1           327-2350
            2530-2756           CR472483.1         1-227               c
            2757-2768           M16409.1           2578-2589
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q24"
     gene            1..2768
                     /gene="Chrm4"
                     /gene_synonym="M4"
                     /note="cholinergic receptor, muscarinic 4"
                     /db_xref="GeneID:25111"
                     /db_xref="RGD:2344"
     exon            1..142
                     /gene="Chrm4"
                     /gene_synonym="M4"
                     /inference="alignment:Splign:2.1.0"
     exon            143..2760
                     /gene="Chrm4"
                     /gene_synonym="M4"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..1611
                     /gene="Chrm4"
                     /gene_synonym="M4"
                     /note="cholinergic receptor, muscarin 4"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M4"
                     /protein_id="NP_113735.1"
                     /db_xref="GeneID:25111"
                     /db_xref="RGD:2344"
                     /translation="MANFTPVNGSSANQSVRLVTAAHNHLETVEMVFIATVTGSLSLV
                     TVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTLYIIKGYWPLGAV
                     VCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSF
                     VLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHIS
                     LASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKPSIKKPPPGGASREELRNGKLEEAPP
                     PALPPPPRPVPDKDTSNESSSGSATQNTKERPPTELSTAEATTPALPAPTLQPRTLNP
                     ASKWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAA
                     RERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPERVWSIGYWLCYVNSTINPA
                     CYALCNATFKKTFRHLLLCQYRNIGTAR"
ORIGIN      
        1 ctgcaggcga agcaggggcc gccccttggc accctgtccc cgcccccggc ccccggccca
       61 gtcgcccacc ccaccgactc gcggaacctc tggcttgttc cgccgtctgt ccggcaccag
      121 cagagaaaga cagaagcagc ggccactgcc cacctgtatc cagttgggtt cagcatggcc
      181 aacttcacgc ctgtcaatgg cagctcagcc aatcagtctg tgcgcctggt cacagcagcc
      241 cacaaccacc tggagacagt ggagatggtg ttcattgcta cagtgactgg ctccctgagc
      301 ctggtgactg ttgtgggtaa catcctggtg atgctgtcca tcaaggtcaa caggcagttg
      361 cagacggtca acaactactt cctcttcagc ctggcgtgtg cggatctcat cataggggca
      421 ttctctatga acctctacac tttgtatatc atcaagggct actggcccct gggtgccgtg
      481 gtctgtgact tgtggctggc cctggactat gtggtgagca atgcctctgt catgaacctt
      541 cttatcatca gcttcgatcg ttacttctgc gtcaccaaac ccctcaccta tcccgcccgc
      601 cgcactacta agatggcagg tctcatgatc gcagctgcct gggtcttgtc ctttgtgctc
      661 tgggctcctg ccatcttgtt ctggcagttt gtggtgggca agaggacggt gcctgataac
      721 cagtgcttca tccagttctt gtccaacccg gcagtgacct ttggcaccgc cattgctgcc
      781 ttctacctgc ctgtggtcat catgacggta ctgtatattc acatctcact ggccagccgc
      841 agccgcgttc acaagcatcg acctgagggc cccaaggaga agaaggccaa gactctggct
      901 ttcctcaaga gccctctgat gaagccgagt atcaagaaac ctccaccagg gggcgcttct
      961 cgagaggaac tgcgcaatgg gaagctggaa gaggctcctc cgccagccct gcccccgcct
     1021 ccacgcccag tgcctgacaa ggacacttcc aatgagtcca gctcaggcag tgccacccag
     1081 aacaccaagg aacggccacc cacagagctg tccaccgcag aggccaccac tccagcgctg
     1141 cctgccccta ccctgcagcc acgaaccctc aacccagcct ccaaatggtc caagattcaa
     1201 attgtaacaa agcagacagg caatgaatgt gtgacggcca tcgagattgt acctgccact
     1261 ccagctggta tgcgcccagc agccaatgtg gcccgaaagt ttgccagcat tgctcgcaac
     1321 caggtgcgca agaagcggca gatggcggcc cgggagcgca aagtgactcg gacaatcttt
     1381 gccattctgc tggccttcat cctcacctgg acaccctaca atgtcatggt cctggtgaac
     1441 accttttgcc agagctgtat ccccgagagg gtgtggtcca tcggctactg gctctgctac
     1501 gtcaacagca ccatcaaccc cgcctgctat gcactctgca atgccacttt caaaaagacc
     1561 ttccggcacc ttttgctgtg ccagtatcgg aacatcggca cagccaggta gacaggcagg
     1621 tgccctagga ggtgctggtg tcatgagtgt gtaccgggga accacatggc tcaccggctg
     1681 tggaggagag agtaggcggc cccactctga gttcgcatct gctgaagaga acaggtctcc
     1741 cagacaccgt gaggtaccag aggcccttcg ctaggatggg agagatctct actgaagcct
     1801 agaaggctca gcccagggag ggtctgaact gtcaccaccc ggcccacatc tgttaccttg
     1861 cctcaggaag atgggggaca atggctggag tgggcagtgg ctctacaaag gccaaccatc
     1921 ggccgaagtg catagagccg agcccaggag catctcggga ggagcaaaag gagccaggct
     1981 tgcaagggcg gggtccccca tgcgctgtaa tcggtgcttt ccgccttcct gcaccctcct
     2041 ccccctgcat gtaggctcag cccttcccct gaccccaagt cccactccag ggtgagagag
     2101 tgccctgcta atgttgctca gccaggctct gggtgggaaa ccaagacctt tggcactccc
     2161 ctggccagca gtcatatctg gatggggcaa ctgggaaatc atgctaagct gagacagatt
     2221 ccagggcact gagtcaatta gtgctggatc atatggtcaa actgtcaaga gcccaccccc
     2281 acccccaggt cacacatcaa atgagtggca gcagcgttcc tctctcctct acaaggaggg
     2341 aggtgtggat ccaagggagg aaaggtggtg gtagggacct tggtgatcta tacaggcctc
     2401 agtgacaggt gacacaagca agcaccctca ttagtcccca aggagagcat tccagactgg
     2461 cagtagcctg gaccagaaag actcttgact aggtgaggca gagttcaaat gagtcacctg
     2521 ggttctcttc cctaacccca gccctgtggg tccctgggag agataacacc caaggggccc
     2581 tggaagacat ttttgtaaat gttctgctct ttccactaat tgttgggagc acttcttacg
     2641 tagccctccg ctcccctcag tacatctttc cccatcctcc cacccccaac aaagctccct
     2701 ggcaaactcc ctgggaaggg gcttttatat attaaaaaag aggcttttat tggaaaaaaa
     2761 aaaaaaaa
//
