LOCUS       NM_012527               4086 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), mRNA.
ACCESSION   NM_012527
VERSION     NM_012527.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Liu,X., Ju,X., Hong,Q., Wang,X., Wu,M., Xing,W., Xu,M., Hu,C. and
            Hao,F.
  TITLE     Establishment of a CaCC-based Cell Model and Method for
            High-throughput Screening of M3 Receptor Drugs
  JOURNAL   Cell Biochem Biophys 81 (1), 49-58 (2023)
   PUBMED   36445617
  REMARK    GeneRIF: Establishment of a CaCC-based Cell Model and Method for
            High-throughput Screening of M3 Receptor Drugs.
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Wu,J., Li,X., Zhou,P. and Li,X.
  TITLE     M3 but not M4 muscarinic receptors in the rostromedial tegmental
            nucleus are involved in the acquisition of morphine-induced
            conditioned place preference
  JOURNAL   Eur J Pharmacol 882, 173274 (2020)
   PUBMED   32534071
  REMARK    GeneRIF: M3 but not M4 muscarinic receptors in the rostromedial
            tegmental nucleus are involved in the acquisition of
            morphine-induced conditioned place preference.
REFERENCE   3  (bases 1 to 4086)
  AUTHORS   Deng,A.Y., deBlois,D., Laporte,S.A., Gelinas,D., Tardif,J.C.,
            Thorin,E., Shi,Y., Raignault,A. and Menard,A.
  TITLE     Novel Pathogenesis of Hypertension and Diastolic Dysfunction Caused
            by M3R (Muscarinic Cholinergic 3 Receptor) Signaling
  JOURNAL   Hypertension 72 (3), 755-764 (2018)
   PUBMED   30354759
  REMARK    GeneRIF: Eliminating muscarinic cholinergic 3 receptor (M3R) from
            Dahl salt sensitive (DSS) rats Improves diastolic dysfunction.
REFERENCE   4  (bases 1 to 4086)
  AUTHORS   Filatova,T.S., Naumenko,N., Galenko-Yaroshevsky,P.A. and
            Abramochkin,D.V.
  TITLE     M3 cholinoreceptors alter electrical activity of rat left atrium
            via suppression of L-type Ca2+ current without affecting K+
            conductance
  JOURNAL   J Physiol Biochem 73 (2), 167-174 (2017)
   PUBMED   27858307
  REMARK    GeneRIF: Data suggest that, in atrial myocardium (or isolated
            cardiomyocytes), activation of muscarinic receptors M3 (Chrm3)
            leads to shortening of action potentials via suppression of L-type
            calcium channels; the major potassium channels are not affected,
            but inwardly rectifying potassium channels are suppressed.
REFERENCE   5  (bases 1 to 4086)
  AUTHORS   Garcia,R.C., Dati,L.M., Torres,L.H., da Silva,M.A., Udo,M.S.,
            Abdalla,F.M., da Costa,J.L., Gorjao,R., Afeche,S.C., Yonamine,M.,
            Niswender,C.M., Conn,P.J., Camarini,R., Sandoval,M.R. and
            Marcourakis,T.
  TITLE     M1 and M3 muscarinic receptors may play a role in the neurotoxicity
            of anhydroecgonine methyl ester, a cocaine pyrolysis product
  JOURNAL   Sci Rep 5, 17555 (2015)
   PUBMED   26626425
  REMARK    GeneRIF: M1 and M3 muscarinic receptors may play a role in the
            neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis
            product.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4086)
  AUTHORS   Wess,J., Maggio,R., Palmer,J.R. and Vogel,Z.
  TITLE     Role of conserved threonine and tyrosine residues in acetylcholine
            binding and muscarinic receptor activation. A study with m3
            muscarinic receptor point mutants
  JOURNAL   J Biol Chem 267 (27), 19313-19319 (1992)
   PUBMED   1527051
REFERENCE   7  (bases 1 to 4086)
  AUTHORS   Wess,J., Gdula,D. and Brann,M.R.
  TITLE     Site-directed mutagenesis of the m3 muscarinic receptor:
            identification of a series of threonine and tyrosine residues
            involved in agonist but not antagonist binding
  JOURNAL   EMBO J 10 (12), 3729-3734 (1991)
   PUBMED   1657592
REFERENCE   8  (bases 1 to 4086)
  AUTHORS   Kurtenbach,E., Curtis,C.A., Pedder,E.K., Aitken,A., Harris,A.C. and
            Hulme,E.C.
  TITLE     Muscarinic acetylcholine receptors. Peptide sequencing identifies
            residues involved in antagonist binding and disulfide bond
            formation
  JOURNAL   J Biol Chem 265 (23), 13702-13708 (1990)
   PUBMED   2380182
REFERENCE   9  (bases 1 to 4086)
  AUTHORS   Braun,T., Schofield,P.R., Shivers,B.D., Pritchett,D.B. and
            Seeburg,P.H.
  TITLE     A novel subtype of muscarinic receptor identified by homology
            screening
  JOURNAL   Biochem Biophys Res Commun 149 (1), 125-132 (1987)
   PUBMED   3120722
REFERENCE   10 (bases 1 to 4086)
  AUTHORS   Bonner,T.I., Buckley,N.J., Young,A.C. and Brann,M.R.
  TITLE     Identification of a family of muscarinic acetylcholine receptor
            genes
  JOURNAL   Science 237 (4814), 527-532 (1987)
   PUBMED   3037705
  REMARK    Erratum:[Science 1987 Sep 25;237(4822):237]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Feb 14, 2013 this sequence version replaced NM_012527.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BF408808.1, SRR26360176.481293.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-310               JAXUCZ010000017.1  64696927-64697236
            311-419             JAXUCZ010000017.1  64818847-64818955
            420-481             JAXUCZ010000017.1  64913178-64913239
            482-528             JAXUCZ010000017.1  65012343-65012389
            529-4086            JAXUCZ010000017.1  65155064-65158621
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.1"
     gene            1..4086
                     /gene="Chrm3"
                     /note="cholinergic receptor, muscarinic 3"
                     /db_xref="GeneID:24260"
                     /db_xref="RGD:2343"
     exon            1..310
                     /gene="Chrm3"
                     /inference="alignment:Splign:2.1.0"
     exon            311..419
                     /gene="Chrm3"
                     /inference="alignment:Splign:2.1.0"
     exon            420..481
                     /gene="Chrm3"
                     /inference="alignment:Splign:2.1.0"
     exon            482..528
                     /gene="Chrm3"
                     /inference="alignment:Splign:2.1.0"
     exon            529..4086
                     /gene="Chrm3"
                     /inference="alignment:Splign:2.1.0"
     CDS             548..2317
                     /gene="Chrm3"
                     /note="cholinergic receptor, muscarinic 3, cardiac"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M3"
                     /protein_id="NP_036659.2"
                     /db_xref="GeneID:24260"
                     /db_xref="RGD:2343"
                     /translation="MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVTQLGSYNISQ
                     ETGNFSSNDTSSDPLGGHTIWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVN
                     NYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLV
                     ISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPG
                     ECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEA
                     ENFVHPTGSSRSCSSYELQQQGVKRSSRRKYGRCHFWFTTKSWKPSAEQMDQDHSSSD
                     SWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSSILNSTKLPSSDNLQVSN
                     EDLGTVDVERNAHKLQAQKSMGDGDNCQKDFTKLPIQLESAVDTGKTSDTNSSADKTT
                     ATLPLSFKEATLAKRFALKTRSQITKRKRMSLIKEKKAAQTLSAILLAFIITWTPYNI
                     MVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLLLCQCDKRK
                     RRKQQYQQRQSVIFHKRVPEQAL"
ORIGIN      
        1 gcggccgcag ctgcccgcgg gcggagcgcg ctcggacccg ggagcgcaac cgcgggccaa
       61 gtcggctgca gccgggagcc ccgcgctcgc gacccgccgg gaggagccgg tagctgcgct
      121 tgcatccagc agaagccgag tgatgaagac aagggcggct ccggggcaca agcgaacctg
      181 aggagagtcg ctgcctcgaa actccctgct gcgagagatc ggggttcggt agagaggacc
      241 ggacggtctg ggagattcgg ggaggcgcgg aggactcgag tgggacagct acctggagca
      301 cggctgccag atatgaccag taatggcatc tacttcagct ctgagttgct cagtgaactg
      361 tggattgact gaaccatatt ctttcccatc atgatagaca acgcaaaggc tctctacttt
      421 gctgcattct gactagtggc caagcacgtg acatcccgaa cttctgcaaa ggtacaataa
      481 gggccgacca tttaattttg gataaccagt tggtgtgttc ttccttggac tatgtcagag
      541 agtcacaatg accttgcaca gtaacagtac aacctcgcct ttgtttccca acatcagctc
      601 ttcctgggtg cacagtccct cggaggcagg gctgcccttg gggacagtca ctcagttggg
      661 cagctacaac atttcacaag aaactgggaa tttctcctca aacgacacct ccagcgaccc
      721 tctcgggggt cacaccatct ggcaagtggt cttcattgcc ttcttaaccg gcttcctggc
      781 attggtgacc atcattggca acatccttgt cattgtggcc ttcaaggtca acaaacagct
      841 gaagacagtc aacaactact tcctcttaag cctggcctgt gcagacctga tcatcggggt
      901 catttccatg aacctgttca ctacctacat cattatgaac cgttgggcac tggggaactt
      961 agcctgcgac ctctggcttt ccattgacta tgtggccagc aatgcctctg tcatgaatct
     1021 gctggtcatc agctttgaca ggtacttttc catcactaga ccactcacct accgagccaa
     1081 aagaacaaca aaacgagctg gtgtgatgat tggtctggct tgggtcatct cctttgtcct
     1141 atgggctcct gccatcttgt tctggcaata ctttgtaggg aagagaactg tgcccccagg
     1201 agaatgtttc attcagtttc tgagtgagcc caccatcacc ttcggcacgg cgatcgctgc
     1261 cttttacatg cctgtcacca tcatgactat tttatactgg aggatctata aggaaactga
     1321 gaagcgtacc aaagagctgg ctggcctaca ggcctctggg acagaagcgg aggcagaaaa
     1381 ctttgtccac cccacaggca gttctcgaag ctgtagcagc tatgaactgc aacagcaagg
     1441 cgtgaaacga tcatccagga ggaagtacgg tcgctgtcac ttctggttca ccaccaagag
     1501 ctggaagccc agtgccgagc agatggacca agaccacagc agcagcgaca gttggaacaa
     1561 caacgatgct gctgcctccc tggaaaactc tgcttcctcc gatgaagagg acattggctc
     1621 agagaccagg gccatctatt ccattgtcct caagcttcca ggccatagct ccatcctcaa
     1681 ctctaccaag ctaccgtcct cagataacct gcaggtgtcc aacgaggacc tggggactgt
     1741 ggatgtggag agaaatgctc acaagcttca ggcccagaag agcatgggtg atggtgacaa
     1801 ctgtcagaag gatttcacca agcttcccat ccagttagag tctgccgtgg acacaggcaa
     1861 gacctctgac accaactcct cggcagacaa gaccacggct actctacctc tgtccttcaa
     1921 ggaggccacg ctggctaaga ggtttgctct caagaccaga agtcagatca ccaagcggaa
     1981 gaggatgtcg ctcatcaagg agaagaaggc cgcccagacg ctcagtgcca tcttgctagc
     2041 cttcatcatc acgtggaccc cctacaacat catggtcctg gtgaacacct tctgtgacag
     2101 ctgcataccc aaaacctatt ggaatctggg ctactggctg tgctatatca acagcaccgt
     2161 gaaccctgtg tgctatgccc tgtgcaacaa aacattcaga accaccttca agacgctcct
     2221 cttgtgccag tgtgacaaaa ggaagaggcg caaacagcag taccagcaga gacagtcggt
     2281 catttttcac aagcgagtgc cggaacaggc cttgtagaaa aggggtatgt tgagagcagt
     2341 gaccacgcaa gcgcgtcagc ccacacagcc tagcaggagt ctggcgcagg tggggggagc
     2401 catccctgat ggtgataaaa tgggttttta tcgacctcac gggagagaag ctacctgttt
     2461 actgatccat tgaataactg attttggtcc aatgccaatt cagcaggaaa gaaggagagg
     2521 cataccgcta aacatgaaga gatgtgttct gaaacagact tttaagtgga ttttgtttcc
     2581 tctaagagaa aaagaaatta ttgtctcaga gcaagtatcc tcagaaattg gtctgcctgg
     2641 gtctcttaat tcctatcagc tctggaatca ctggtgagcc tcaaggcact agatgccatg
     2701 tgctctccct aagggtccca aagtgtccat ccagatccca tgtgaagcac ggcaagcttg
     2761 aaaaaaaaaa tctgtggtcc tgagttattt tgtatattgc aaagctgaca ccacttgtcc
     2821 ccaatcagct tctgtcctct ttcagtgtgt tgtcaaactc tattcgtgga tttgactctt
     2881 ggttcttgca acgtactgtt tcgtgctgtt caagtttcac acaaataaaa ttgtacagga
     2941 acatgcatac ctatacatgt gtgagtttcc gcacgcacgc acattgtgta tataacgtga
     3001 tggggaacac tgaactgaaa ggaagttcct accatacttt ggtaactgaa actctctagg
     3061 atcctcaaat gatatgaaac tgaactaagc ccagtttgac gttgtgtttt ggtctgggtt
     3121 tttgacactg gagctatttc ccacaaacca tcctgaagaa taatatccag cacagaaaat
     3181 ccagaccttc tcagcaaacc tgtgctagac agacagtctt gagggtcagt cggcaccgtg
     3241 tcttgactgg actcagtacg cactgtctcc tgcactgtta gctctctgtc gatggagcta
     3301 aataaaccag ggcccctttg agctcaaaag aacgaacttc tgtacatgtt tgaatttagt
     3361 cgtccaaatc tgaatgtttc aaaacaaaat ttctgggatc tgaactgctc gaaactcaat
     3421 cctagagtca cctttgaatg tcacacacag caatataatc tacatttaca tagaaaaatc
     3481 aaaggaaacg ctgtgctcgg agctgacgga ggaaagtatt gtattccact tcctgctacc
     3541 tggcaccttc tgaaaggaag gtgggcttac ctcctgtcac ttagcaggag tcagaaccct
     3601 tctggtagtc actgtataac cttcacctag gagtggttgg tgtctaggaa gccatgcatc
     3661 ctctgaagaa agaatggcac tagaaaaagt attacttcga aatcgatttc tttccattgt
     3721 gacaaccgaa tttcgcatca tcctttgaat tgccccaagt gcaccgtgtt tcctctgggg
     3781 gaggggtctg ctgctctcat tcttctggct gctgtattgt tgggtttctt cttttccttc
     3841 tgctgggact gtgtggggag cgggtggggt gggggggtgg gagggctgtg gagaaccctt
     3901 cccttgtgca gggcatccat ccgtgttgtg aacccagagc tgggggccaa agctgctttt
     3961 gtattcagtg tgaggtggtg tttacagccg actttgacat cagtgggtgt gttctcagtg
     4021 gtgtgtctgt acctgaacta tttaatgttg tgtcatgttt atataaagaa tatttatgca
     4081 tacttt
//
