LOCUS       NM_001398586            2571 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus choline kinase alpha (Chka), transcript variant
            1, mRNA.
ACCESSION   NM_001398586 XM_006230708
VERSION     NM_001398586.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Gallego-Ortega,D., Ramirez de Molina,A., Ramos,M.A.,
            Valdes-Mora,F., Barderas,M.G., Sarmentero-Estrada,J. and Lacal,J.C.
  TITLE     Differential role of human choline kinase alpha and beta enzymes in
            lipid metabolism: implications in cancer onset and treatment
  JOURNAL   PLoS One 4 (11), e7819 (2009)
   PUBMED   19915674
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Tian,Y., Jackson,P., Gunter,C., Wang,J., Rock,C.O. and Jackowski,S.
  TITLE     Placental thrombosis and spontaneous fetal death in mice deficient
            in ethanolamine kinase 2
  JOURNAL   J Biol Chem 281 (38), 28438-28449 (2006)
   PUBMED   16861741
REFERENCE   3  (bases 1 to 2571)
  AUTHORS   Sher,R.B., Aoyama,C., Huebsch,K.A., Ji,S., Kerner,J., Yang,Y.,
            Frankel,W.N., Hoppel,C.L., Wood,P.A., Vance,D.E. and Cox,G.A.
  TITLE     A rostrocaudal muscular dystrophy caused by a defect in choline
            kinase beta, the first enzyme in phosphatidylcholine biosynthesis
  JOURNAL   J Biol Chem 281 (8), 4938-4948 (2006)
   PUBMED   16371353
REFERENCE   4  (bases 1 to 2571)
  AUTHORS   Rickhag,M., Wieloch,T., Gido,G., Elmer,E., Krogh,M., Murray,J.,
            Lohr,S., Bitter,H., Chin,D.J., von Schack,D., Shamloo,M. and
            Nikolich,K.
  TITLE     Comprehensive regional and temporal gene expression profiling of
            the rat brain during the first 24 h after experimental stroke
            identifies dynamic ischemia-induced gene expression patterns, and
            reveals a biphasic activation of genes in surviving tissue
  JOURNAL   J Neurochem 96 (1), 14-29 (2006)
   PUBMED   16300643
REFERENCE   5  (bases 1 to 2571)
  AUTHORS   Liu,Y., Blum,P.S., Pabst,D.M., Chakrabarti,I. and Jernigan,H.M. Jr.
  TITLE     Effects of cataractogenesis on the CDP-choline pathway: changes in
            ATP concentration and phosphocholine synthesis during and after
            exposure of rat lenses to sugars in vitro and in vivo
  JOURNAL   Ophthalmic Res 35 (4), 185-191 (2003)
   PUBMED   12815193
REFERENCE   6  (bases 1 to 2571)
  AUTHORS   Aoyama,C., Ohtani,A. and Ishidate,K.
  TITLE     Expression and characterization of the active molecular forms of
            choline/ethanolamine kinase-alpha and -beta in mouse tissues,
            including carbon tetrachloride-induced liver
  JOURNAL   Biochem J 363 (Pt 3), 777-784 (2002)
   PUBMED   11964179
REFERENCE   7  (bases 1 to 2571)
  AUTHORS   Nakagami,K., Uchida,T., Ohwada,S., Koibuchi,Y. and Morishita,Y.
  TITLE     Increased choline kinase activity in 1,2-dimethylhydrazine-induced
            rat colon cancer
  JOURNAL   Jpn J Cancer Res 90 (11), 1212-1217 (1999)
   PUBMED   10622531
REFERENCE   8  (bases 1 to 2571)
  AUTHORS   Nakagami,K., Uchida,T., Ohwada,S., Koibuchi,Y., Suda,Y., Sekine,T.
            and Morishita,Y.
  TITLE     Increased choline kinase activity and elevated phosphocholine
            levels in human colon cancer
  JOURNAL   Jpn J Cancer Res 90 (4), 419-424 (1999)
   PUBMED   10363580
REFERENCE   9  (bases 1 to 2571)
  AUTHORS   Uchida,T.
  TITLE     Regulation of choline kinase R: analyses of alternatively spliced
            choline kinases and the promoter region
  JOURNAL   J Biochem 116 (3), 508-518 (1994)
   PUBMED   7852267
REFERENCE   10 (bases 1 to 2571)
  AUTHORS   Uchida,T. and Yamashita,S.
  TITLE     Molecular cloning, characterization, and expression in Escherichia
            coli of a cDNA encoding mammalian choline kinase
  JOURNAL   J Biol Chem 267 (14), 10156-10162 (1992)
   PUBMED   1577786
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Dec 20, 2021 this sequence version replaced XM_006230708.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D37885.1, SRR22399484.1024040.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-582               JAXUCZ010000001.1  210506115-210506696
            583-694             JAXUCZ010000001.1  210520746-210520857
            695-748             JAXUCZ010000001.1  210533299-210533352
            749-862             JAXUCZ010000001.1  210542275-210542388
            863-996             JAXUCZ010000001.1  210546432-210546565
            997-1101            JAXUCZ010000001.1  210546962-210547066
            1102-1160           JAXUCZ010000001.1  210548005-210548063
            1161-1248           JAXUCZ010000001.1  210550271-210550358
            1249-1357           JAXUCZ010000001.1  210551116-210551224
            1358-1464           JAXUCZ010000001.1  210552518-210552624
            1465-1546           JAXUCZ010000001.1  210553107-210553188
            1547-2571           JAXUCZ010000001.1  210553729-210554753
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..2571
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /note="choline kinase alpha"
                     /db_xref="GeneID:29194"
                     /db_xref="RGD:61944"
     exon            1..582
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     CDS             245..1606
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /EC_number="2.7.1.32"
                     /EC_number="2.7.1.82"
                     /note="isoform 1 is encoded by transcript variant 1; CK;
                     CHETK-alpha; EK; ethanolamine kinase; choline kinase R"
                     /codon_start=1
                     /product="choline kinase alpha isoform 1"
                     /protein_id="NP_001385515.1"
                     /db_xref="GeneID:29194"
                     /db_xref="RGD:61944"
                     /translation="MKTKFCTGGEAEPSPLGLLLSCGGSAAPTPGVGQQRDAAGELES
                     KQLGGRSQPLALPPPPPPPLPLPPPPSPPLADEQPEPRTRRRAYLWCKEFLPGAWRGL
                     REDQFHISVIRGGLSNMLFQCSLPDSIASVGDEPRKVLLRLYGAILKMRSCNKEGSEQ
                     AQNENEFQGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELCLP
                     DISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLNQVLRLKFSREARVQQLHKFLSY
                     NLPLELENLRSLLQYTRSPVVFCHNDCQEGNILLLEGQENSEKQKLMLIDFEYSSYNY
                     RGFDIGNHFCEWMYDYTYEKYPFFRANIQKYPTRKQQLHFISSYLTTFQNDFESLSSE
                     EQSATKEDMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMEYAQARFDAYFDQKRK
                     LGV"
     exon            583..694
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            695..748
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            749..862
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            863..996
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1101
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1160
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1248
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1357
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1464
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1546
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..2571
                     /gene="Chka"
                     /gene_synonym="Chk; CK-R"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcagtcgcc cgccgtcagc ctcccgcgct cgtctctcgt cactgctgct cggcgtccat
       61 tgctgcctct ccccgcagtc gccgacgtcg cttccccgcg cgctcccaca accgccgccc
      121 cgccggtcag tgaagccggt gagccattcc ccgcgccggc ccccagaggc gggcatccag
      181 ccggaccccg agtgtggccc tctcctgctg tggccgtccg cgccttctcg accgcttatc
      241 cagcatgaaa accaagttct gcaccggggg cgaggccgag ccgtccccgc ttgggctgct
      301 gctgagctgc ggtggcagcg ctgccccgac gcccggcgta gggcagcagc gcgatgccgc
      361 aggcgagctg gagtccaagc agcttggtgg ccggtcccaa cctctcgcgc tgccgccgcc
      421 accaccgccg cccctgccgc tgcccccgcc gccatcaccg ccgctagcgg acgaacaacc
      481 cgagccccgg acgcggcgca gggcctacct gtggtgcaag gaattcctgc ccggagcctg
      541 gaggggcctt cgcgaggacc agttccacat cagtgtcatc aggggtggtc tcagtaacat
      601 gctgttccag tgttccttgc cagactccat agccagtgtt ggtgatgaac ctcggaaagt
      661 gctcttgcga ctgtatgggg caatcttaaa gatgaggtcc tgtaataaag agggatccga
      721 acaagctcag aatgaaaatg aatttcaagg ggctgaagca atggttctgg agagtgttat
      781 gtttgccatt cttgcagaga ggtcacttgg gccaaaactc tatggcatct ttccgcaagg
      841 ccgactggag cagtttatcc cgagccggcg attggacact gaagaattat gtttaccaga
      901 tatttctgca gaaatagctg aaaaaatggc cacatttcat ggtatgaaaa tgccattcaa
      961 taaggaacca aaatggcttt ttggaacaat ggaaaaatac ctgaatcaag tactaagact
     1021 taaattcagc agggaggcca gagttcaaca actgcacaag ttcctctctt acaatctgcc
     1081 tctcgagctt gagaacctga ggtcattgct gcagtatact agatccccag ttgtgttttg
     1141 tcataatgac tgtcaagaag gtaatatctt attgttggaa ggccaagaga attctgaaaa
     1201 gcagaagttg atgctcattg actttgaata cagcagttac aattacaggg gatttgacat
     1261 tggaaatcat ttctgtgaat ggatgtatga ttatacctat gaaaagtatc ctttcttcag
     1321 agcaaacatt cagaagtatc ctacccgaaa acaacagctc cattttattt caagttactt
     1381 gactacattc caaaatgatt ttgaaagcct cagcagtgaa gagcagtctg ctacaaaaga
     1441 agacatgttg cttgaagtca acagatttgc ccttgcctct catttcctct ggggactttg
     1501 gtccattgta caggccaaga tctcatccat tgaatttggg tacatggaat atgcccaagc
     1561 caggttcgat gcctactttg accagaagag gaagcttggt gtgtgaatgg atggctccac
     1621 tcttcaccac tggactgcag gaggtggctg caccaggccc tcagtggagc gctgctgtga
     1681 ccactgccct gggcagaagg cctggacgtc tcactactga gcaccgatgt gtatgatact
     1741 acagactata ttaaagtgga gtaacatttc tttcatcttt gtttacactc tcactaggac
     1801 tctgaaccat gattggaagc agaaatatag tgtgatagtg caatagctca gaccccgcct
     1861 aagcgggagg cctttcagct acatggctac agcttcagcc acttaggccc cagccagaca
     1921 gagcagtgct gtgtgggtac tgagtgctga cttaggatat taatgtgctg caacacgttc
     1981 atgaccaggc tttgaaggtg acagtctgac aatgtgttgg agacactctg aagggcaagt
     2041 gaacagacat actgtgaaat ggctcgacag gaggagcctg aattgtgggg tctgtggagg
     2101 cagccagctg tttctgtaca gggtacactt gactatgggt atgcatctgc aggcagtagc
     2161 tgcagccctc ctgtgcctgt gtacacatga ctacaggggc cagtgtcact gactggccat
     2221 aactgcagtg tctcctaact gggtgtgctt tatgcttcag cttcccgggg aggagcagtg
     2281 gagccagctt cctcacccct tcttgccttc tctctgcctg acctggaact tgggctttcg
     2341 cccattgccc tctgaagctg cttcccatct gatgtcactg ggagacagca gctgtatgtg
     2401 tggggtattg gggtgcaggt agattagagc tgtgaaatcc atgtacatta atacccaatg
     2461 ggataaacct agaatttttt ttttttactc tgaactctga attgttttgt gcacatattt
     2521 ctgctaccac cgaaactgta ttatacagat aaataaacaa cttgaaactt a
//
