LOCUS       NM_053677               1749 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus checkpoint kinase 2 (Chek2), mRNA.
ACCESSION   NM_053677
VERSION     NM_053677.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Girard E, Eon-Marchais S, Olaso R, Renault AL, Damiola F, Dondon
            MG, Barjhoux L, Goidin D, Meyer V, Le Gal D, Beauvallet J, Mebirouk
            N, Lonjou C, Coignard J, Marcou M, Cavaciuti E, Baulard C, Bihoreau
            MT, Cohen-Haguenauer O, Leroux D, Penet C, Fert-Ferrer S, Colas C,
            Frebourg T, Eisinger F, Adenis C, Fajac A, Gladieff L, Tinat J,
            Floquet A, Chiesa J, Giraud S, Mortemousque I, Soubrier F,
            Audebert-Bellanger S, Limacher JM, Lasset C, Lejeune-Dumoulin S,
            Dreyfus H, Bignon YJ, Longy M, Pujol P, Venat-Bouvet L, Bonadona V,
            Berthet P, Luporsi E, Maugard CM, Nogues C, Delnatte C, Fricker JP,
            Gesta P, Faivre L, Lortholary A, Buecher B, Caron O,
            Gauthier-Villars M, Coupier I, Servant N, Boland A, Mazoyer S,
            Deleuze JF, Stoppa-Lyonnet D, Andrieu N and Lesueur F.
  TITLE     Familial breast cancer and DNA repair genes: Insights into known
            and novel susceptibility genes from the GENESIS study, and
            implications for multigene panel testing
  JOURNAL   Int J Cancer 144 (8), 1962-1974 (2019)
   PUBMED   30303537
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Xin F, Jiang L, Liu X, Geng C, Wang W, Zhong L, Yang G and Chen M.
  TITLE     Bisphenol A induces oxidative stress-associated DNA damage in INS-1
            cells
  JOURNAL   Mutat Res Genet Toxicol Environ Mutagen 769, 29-33 (2014)
   PUBMED   25344109
REFERENCE   3  (bases 1 to 1749)
  AUTHORS   Khoury-Haddad H, Guttmann-Raviv N, Ipenberg I, Huggins D,
            Jeyasekharan AD and Ayoub N.
  TITLE     PARP1-dependent recruitment of KDM4D histone demethylase to DNA
            damage sites promotes double-strand break repair
  JOURNAL   Proc Natl Acad Sci U S A 111 (7), E728-E737 (2014)
   PUBMED   24550317
REFERENCE   4  (bases 1 to 1749)
  AUTHORS   Takahashi,K., Takatsu,M., Hattori,T., Murase,T., Ohura,S.,
            Takeshita,Y., Watanabe,S., Murohara,T. and Nagata,K.
  TITLE     Premature cardiac senescence in DahlS.Z-Lepr(fa)/Lepr(fa) rats as a
            new animal model of metabolic syndrome
  JOURNAL   Nagoya J Med Sci 76 (1-2), 35-49 (2014)
   PUBMED   25129990
REFERENCE   5  (bases 1 to 1749)
  AUTHORS   Nguyen CL, Possemato R, Bauerlein EL, Xie A, Scully R and Hahn WC.
  TITLE     Nek4 regulates entry into replicative senescence and the response
            to DNA damage in human fibroblasts
  JOURNAL   Mol Cell Biol 32 (19), 3963-3977 (2012)
   PUBMED   22851694
REFERENCE   6  (bases 1 to 1749)
  AUTHORS   Bartkova J, Falck J, Rajpert-De Meyts E, Skakkebaek NE, Lukas J and
            Bartek J.
  TITLE     Chk2 tumour suppressor protein in human spermatogenesis and
            testicular germ-cell tumours
  JOURNAL   Oncogene 20 (41), 5897-5902 (2001)
   PUBMED   11593395
REFERENCE   7  (bases 1 to 1749)
  AUTHORS   Hu B, Zhou XY, Wang X, Zeng ZC, Iliakis G and Wang Y.
  TITLE     The radioresistance to killing of A1-5 cells derives from
            activation of the Chk1 pathway
  JOURNAL   J Biol Chem 276 (21), 17693-17698 (2001)
   PUBMED   11278490
REFERENCE   8  (bases 1 to 1749)
  AUTHORS   Falck J, Mailand N, Syljuasen RG, Bartek J and Lukas J.
  TITLE     The ATM-Chk2-Cdc25A checkpoint pathway guards against
            radioresistant DNA synthesis
  JOURNAL   Nature 410 (6830), 842-847 (2001)
   PUBMED   11298456
REFERENCE   9  (bases 1 to 1749)
  AUTHORS   Hirao A, Kong YY, Matsuoka S, Wakeham A, Ruland J, Yoshida H, Liu
            D, Elledge SJ and Mak TW.
  TITLE     DNA damage-induced activation of p53 by the checkpoint kinase Chk2
  JOURNAL   Science 287 (5459), 1824-1827 (2000)
   PUBMED   10710310
REFERENCE   10 (bases 1 to 1749)
  AUTHORS   Chaturvedi P, Eng WK, Zhu Y, Mattern MR, Mishra R, Hurle MR, Zhang
            X, Annan RS, Lu Q, Faucette LF, Scott GF, Li X, Carr SA, Johnson
            RK, Winkler JD and Zhou BB.
  TITLE     Mammalian Chk2 is a downstream effector of the ATM-dependent DNA
            damage checkpoint pathway
  JOURNAL   Oncogene 18 (28), 4047-4054 (1999)
   PUBMED   10435585
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF134054.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF134054.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q16"
     gene            1..1749
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /note="checkpoint kinase 2"
                     /db_xref="GeneID:114212"
                     /db_xref="RGD:621543"
     exon            1..88
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            89..423
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1733
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /EC_number="2.7.11.1"
                     /note="protein kinase Chk2; CHK2 checkpoint homolog"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Chk2"
                     /protein_id="NP_446129.1"
                     /db_xref="GeneID:114212"
                     /db_xref="RGD:621543"
                     /translation="MKSHQSHGSTSSKAHDSASCSQSQGGFSQSQGTPSQLHDLSQYQ
                     GASSSSTSTVPSSSQSSHSSSGTLSSLETVSTQELCSIPEDQEPEEPGPTPWARLWAL
                     QDGFSNLDCVNDNYWFGRDKSCEYCFDGPLLKRTDKYRTYSKKHFRIFREMGPKNCYI
                     VYLEDHSGNGTFVNTELIGKGKRCPLSNNSEIALSLCRNKVFVFFDLTVDDQSVYPKE
                     LRDEYIMSKTLGSGACGEVKMAFERKTCKKVAIKIISKRRFALGSSREADTAPSVETE
                     IEILKKLNHPCIIKIKDVFDVEDYYIVLELMEGGELFDRVVGNKRLKEATCKLYFYQM
                     LLAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGQSKILGETSLMRTLCGTPT
                     YLAPEVLISNGTAGYSRAVDCWSLGVILFICLSGYPPFSEHKTQVSLKDQITSGKYNL
                     IPEVWTDVSEKALDLVKKLLVVDPKARLTTEEALSHPWLQDEHMKKKFQDLLVQEKNL
                     VPLPLAPAQTSGQKRPLELEAEDAESSKRLAVCKAVL"
     exon            424..548
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            549..696
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            697..787
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            788..896
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            897..950
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1012
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1112
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1199
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1363
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1479
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1565
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1646
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1749
                     /gene="Chek2"
                     /gene_synonym="Chk2; Rad53"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcagcatacg tgtggggtag gatggcaagg aatggatagg agacaaccaa acagcatctg
       61 tacatgaagg aagatggtct aacgctttgt cggatatgaa gagtcatcag tcccacggca
      121 gcacctcctc gaaggctcat gacagtgctt cctgttcaca gtctcaaggc ggcttcagcc
      181 agtcccaagg caccccctct cagttgcacg acctctcaca gtatcagggt gcatccagtt
      241 cctccaccag cacagtgcca tcctccagcc agtcctccca ctccagctct gggaccctga
      301 gctctttaga gacagtgtct actcaggaac tctgttctat tcctgaggac caagaaccgg
      361 aagagcctgg acctactcct tgggctcggc tatgggctct tcaggatgga ttctcgaatc
      421 tagactgtgt taatgacaac tactggttcg ggagggataa aagctgtgaa tattgcttcg
      481 atggaccact gttgaaaagg acggataagt accggactta cagcaagaag cactttcgca
      541 tcttcaggga aatgggccct aagaattgtt acattgtata cctagaagac cacagtggaa
      601 atggaacctt tgtaaataca gagcttatcg ggaaaggaaa acgctgtcct ttgagtaaca
      661 actctgaaat cgcactttca ctgtgtagaa ataaagtttt tgtatttttt gatctgactg
      721 tagatgacca gtcagtttat cctaaggaat taagagacga atacatcatg tcaaaaactc
      781 ttggaagcgg tgcctgtggg gaggtaaaga tggcctttga gaggaaaacg tgtaagaaag
      841 tggccataaa gatcatcagc aagcggaggt tcgcccttgg ctcgtccaga gaagctgaca
      901 cagctcccag tgtggaaact gaaatagaaa ttttgaagaa gctgaatcat ccgtgcatca
      961 tcaagattaa agatgtcttt gatgtggaag attattacat tgttctggaa ctgatggaag
     1021 gaggagaatt gtttgaccgt gtggtgggga acaaacgcct gaaggaagcc acctgcaagc
     1081 tctatttcta ccaaatgctt ttagctgtac agtaccttca tgaaaatggg atcatacatc
     1141 gggacctaaa gccagagaat gttcttctgt catctcagga agaggactgt cttatcaaga
     1201 tcactgattt tggacagtcc aagatcttgg gggagacctc cctgatgagg actctatgtg
     1261 gtacgcccac ttatctggct cctgaggtcc ttatctccaa cgggactgct gggtacagcc
     1321 gggccgtcga ctgctggagc ctaggagtca ttcttttcat ctgcctaagt gggtatccac
     1381 ctttctctga gcataagacc caagtgtcac tgaaggatca gatcaccagt ggaaagtaca
     1441 accttattcc ggaagtctgg acagatgtct cagagaaggc tctggacctt gtcaagaaac
     1501 tactggttgt ggatccaaag gccaggctta ccacagagga agccttaagt catccttggc
     1561 ttcaggatga gcacatgaag aagaagtttc aggatctgct ggtgcaggaa aagaacttgg
     1621 tgcccctccc tctggctcct gcccagactt ccggtcaaaa gcggcccctg gaactggagg
     1681 cggaggacgc ggagagctcg aagcgcctgg ccgtgtgtaa ggctgtgttg tgagcgttgg
     1741 acatgaaag
//
