LOCUS       NM_080400               2336 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus checkpoint kinase 1 (Chek1), mRNA.
ACCESSION   NM_080400
VERSION     NM_080400.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Tan,X., Liang,R.Y. and Chuang,S.M.
  TITLE     hHR23A is required to control the basal turnover of Chk1
  JOURNAL   Cell Signal 27 (11), 2304-2313 (2015)
   PUBMED   26296656
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Park,C., Suh,Y. and Cuervo,A.M.
  TITLE     Regulated degradation of Chk1 by chaperone-mediated autophagy in
            response to DNA damage
  JOURNAL   Nat Commun 6, 6823 (2015)
   PUBMED   25880015
  REMARK    GeneRIF: Chaperone-mediated autophagy, in response to genotoxic
            stress, is regulated by degradation of activated Chk1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2336)
  AUTHORS   Ajay,A.K., Kim,T.M., Ramirez-Gonzalez,V., Park,P.J., Frank,D.A. and
            Vaidya,V.S.
  TITLE     A bioinformatics approach identifies signal transducer and
            activator of transcription-3 and checkpoint kinase 1 as upstream
            regulators of kidney injury molecule-1 after kidney injury
  JOURNAL   J Am Soc Nephrol 25 (1), 105-118 (2014)
   PUBMED   24158981
  REMARK    GeneRIF: KIM-1 expression increased significantly in a manner that
            corresponded temporally and regionally with increased
            phosphorylation of checkpoint kinase 1 (Chk1) and STAT3 in acute
            kidney injury.
REFERENCE   4  (bases 1 to 2336)
  AUTHORS   Bischof,J., Randoll,S.J., Sussner,N., Henne-Bruns,D., Pinna,L.A.
            and Knippschild,U.
  TITLE     CK1delta kinase activity is modulated by Chk1-mediated
            phosphorylation
  JOURNAL   PLoS One 8 (7), e68803 (2013)
   PUBMED   23861943
  REMARK    GeneRIF: Casein kinase 1 delta activity is modulated by
            Chk1-mediated phosphorylation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2336)
  AUTHORS   Pieragostino,D., Agnifili,L., Fasanella,V., D'Aguanno,S.,
            Mastropasqua,R., Di Ilio,C., Sacchetta,P., Urbani,A. and Del
            Boccio,P.
  TITLE     Shotgun proteomics reveals specific modulated protein patterns in
            tears of patients with primary open angle glaucoma naive to therapy
  JOURNAL   Mol Biosyst 9 (6), 1108-1116 (2013)
   PUBMED   23580065
REFERENCE   6  (bases 1 to 2336)
  AUTHORS   Sutherland,H.G., Mumford,G.K., Newton,K., Ford,L.V., Farrall,R.,
            Dellaire,G., Caceres,J.F. and Bickmore,W.A.
  TITLE     Large-scale identification of mammalian proteins localized to
            nuclear sub-compartments
  JOURNAL   Hum Mol Genet 10 (18), 1995-2011 (2001)
   PUBMED   11555636
REFERENCE   7  (bases 1 to 2336)
  AUTHORS   Hu,B., Zhou,X.Y., Wang,X., Zeng,Z.C., Iliakis,G. and Wang,Y.
  TITLE     The radioresistance to killing of A1-5 cells derives from
            activation of the Chk1 pathway
  JOURNAL   J Biol Chem 276 (21), 17693-17698 (2001)
   PUBMED   11278490
REFERENCE   8  (bases 1 to 2336)
  AUTHORS   Liu,Q., Guntuku,S., Cui,X.S., Matsuoka,S., Cortez,D., Tamai,K.,
            Luo,G., Carattini-Rivera,S., DeMayo,F., Bradley,A., Donehower,L.A.
            and Elledge,S.J.
  TITLE     Chk1 is an essential kinase that is regulated by Atr and required
            for the G(2)/M DNA damage checkpoint
  JOURNAL   Genes Dev 14 (12), 1448-1459 (2000)
   PUBMED   10859164
REFERENCE   9  (bases 1 to 2336)
  AUTHORS   Takai,H., Tominaga,K., Motoyama,N., Minamishima,Y.A., Nagahama,H.,
            Tsukiyama,T., Ikeda,K., Nakayama,K., Nakanishi,M. and Nakayama,K.
  TITLE     Aberrant cell cycle checkpoint function and early embryonic death
            in Chk1(-/-) mice
  JOURNAL   Genes Dev 14 (12), 1439-1447 (2000)
   PUBMED   10859163
REFERENCE   10 (bases 1 to 2336)
  AUTHORS   Flaggs,G., Plug,A.W., Dunks,K.M., Mundt,K.E., Ford,J.C.,
            Quiggle,M.R., Taylor,E.M., Westphal,C.H., Ashley,T., Hoekstra,M.F.
            and Carr,A.M.
  TITLE     Atm-dependent interactions of a mammalian chk1 homolog with meiotic
            chromosomes
  JOURNAL   Curr Biol 7 (12), 977-986 (1997)
   PUBMED   9382850
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Nov 26, 2020 this sequence version replaced NM_080400.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF414135.1, AF443592.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                JAXUCZ010000008.1  44629819-44629843   c
            26-107              JAXUCZ010000008.1  44629028-44629109   c
            108-331             JAXUCZ010000008.1  44626979-44627202   c
            332-396             JAXUCZ010000008.1  44625979-44626043   c
            397-466             JAXUCZ010000008.1  44625820-44625889   c
            467-655             JAXUCZ010000008.1  44619292-44619480   c
            656-760             JAXUCZ010000008.1  44618166-44618270   c
            761-856             JAXUCZ010000008.1  44615991-44616086   c
            857-965             JAXUCZ010000008.1  44614566-44614674   c
            966-1143            JAXUCZ010000008.1  44613820-44613997   c
            1144-1275           JAXUCZ010000008.1  44613472-44613603   c
            1276-1377           JAXUCZ010000008.1  44612258-44612359   c
            1378-2336           JAXUCZ010000008.1  44609417-44610375   c
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     gene            1..2336
                     /gene="Chek1"
                     /note="checkpoint kinase 1"
                     /db_xref="GeneID:140583"
                     /db_xref="RGD:620545"
     exon            1..25
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            26..107
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..1473
                     /gene="Chek1"
                     /EC_number="2.7.11.1"
                     /note="checkpoint kinase 1 homolog; CHK1 checkpoint
                     homolog"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Chk1"
                     /protein_id="NP_536325.1"
                     /db_xref="GeneID:140583"
                     /db_xref="RGD:620545"
                     /translation="MAVPFVEDWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKR
                     AIDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGM
                     PEQDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRHNNR
                     ERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEY
                     SDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLNRGAKRP
                     RATSGGMSESSSGFSKHIHSNLDFSPINSGSSEENVKFSSSQPEPRTGLSLWDTGPSN
                     VDKLVQGISFSQPTCPDHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQC
                     LKETFEKLGYQWKKSCMNQVTVSTMDRRNNKLIFKINLVEMDEKILVDFRLSKGDGLE
                     FKRHFLKIKGKLSDIVSSQKVWFPVT"
     exon            108..331
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            332..396
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            397..466
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            467..655
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            656..760
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            761..856
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..965
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1143
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1275
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1377
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..2336
                     /gene="Chek1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtggcagctg gcaaaggact gcttgttgct gtgcttggag tcatggcagt gccttttgtg
       61 gaagactggg atttggtgca aactttggga gaaggcgcct atggagaagt tcaacttgct
      121 gtgaatagaa taactgaaga agctgttgca gtgaaaattg tagacatgaa gcgggccata
      181 gactgcccag aaaatattaa gaaagagatc tgcatcaata agatgttaaa tcatgagaat
      241 gtagtgaaat tctatggcca caggagggaa ggcaacatcc agtacctgtt tctggagtac
      301 tgtagtggag gagaactctt tgatagaata gagccagaca tagggatgcc tgaacaagat
      361 gctcagaggt ttttccatca gctcatggcg ggggtggttt atcttcatgg cattggaata
      421 actcacaggg atattaaacc agaaaacctc ctcttggatg aaagggataa tctcaaaatc
      481 tctgactttg gcttggcaac agtgtttcgg cataataatc gtgagcgctt actgaacaag
      541 atgtgtggga ctttacctta tgtcgctcca gagcttctaa agaggaaaga atttcacgca
      601 gaaccagttg atgtttggtc ctgtggaata gtgcttactg caatgttggc tggagaattg
      661 ccttgggacc agcccagtga tagctgtcaa gaatattctg attggaaaga aaagaaaacc
      721 tatctcaatc cttggaaaaa aattgattct gctcctctgg ctttgcttca taaaattcta
      781 gttgagaatc catcagcaag gatcaccatc ccagacatta aaaaagatag atggtacaac
      841 aaaccactta acagaggagc gaagaggcca cgtgctactt caggtggtat gtcagagtct
      901 tctagtggat tctctaagca cattcattcc aatttggact tttctccaat aaatagtggt
      961 tccagtgaag aaaacgtgaa gttctctagt tcccagccag agcctcggac agggctttcc
     1021 ttgtgggaca ctggcccctc aaatgtggac aaactggtgc aaggcatcag tttttcccag
     1081 cctacgtgtc ctgatcatat gcttgtaaac agtcagctac ttggtacccc tggatcttca
     1141 cagaacccct ggcagcgctt ggtcaaaagg atgacacgat tcttcactaa attggatgca
     1201 gacaaatctt accaatgcct gaaagagacc tttgagaagt tgggctatca gtggaagaag
     1261 agttgtatga atcaggttac tgtatcaaca atggatagaa gaaacaataa gttgattttc
     1321 aaaataaatt tggtagaaat ggatgagaag atactggttg acttccggct ttctaagggt
     1381 gatgggttag agttcaagag acacttcctg aagattaaag ggaagctcag cgatatcgtg
     1441 agcagccaga aggtttggtt ccctgttacg tgacgaagcc gtcagctctg ctcatccctg
     1501 gggaatccag tgctgctgct gtgggacatt tttcttccta gaggaggtta tcttattctg
     1561 caaactgcaa acagtagttg tcgaagaact ctgtgcccat tacccaaaca tcttccagtt
     1621 cctagtatgt tttgcgtaca aatcctactg tatcttgatt gtatgtaata ctttggggaa
     1681 aggatggatc aaattcatta ggtatttctc cagctgtgtt taaattgtct gaatttgaaa
     1741 ccgagttttg ggatgcttga gttttccagc ttttatacca atgtagtatc aattttgaac
     1801 agccaaaagt ttgtttcatc ccaagcaaac tattctttct ctgcctgttt agttgtaagg
     1861 atgaataaac acagaccatg tacagttgat tggttcgtga gtgaggccag ccccagaaag
     1921 tatgtatgca cgtactgtaa tttcagcttg ggtgtacgct gcacaagggc ttgaccaatt
     1981 ataaactgtt ttgagttaga attgccccgc cctgccccgc ccctagttta gctagtcaaa
     2041 taagaagcct gatttggttg gtctttcaat gactttactc taaaaattac acacttaact
     2101 atttttttct acattcacag ccatatttta gctactctag aaataaactc tgttttcaaa
     2161 atcaataccc aaatcggtga actgactcgg ccaccatgtt tctagatgac actgactaag
     2221 aaggggatgg gggagtaaag aaatgcagga gttggcctga ttcttgttcc tcacattgta
     2281 gactcactaa aactttgtcg tgttcataaa atttctatta aaatgtgaat atatga
//
