LOCUS       NM_001347661            8400 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus chromodomain helicase DNA binding protein 8
            (Chd8), mRNA.
ACCESSION   NM_001347661
VERSION     NM_001347661.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Zhao,C., Dong,C., Frah,M., Deng,Y., Marie,C., Zhang,F., Xu,L.,
            Ma,Z., Dong,X., Lin,Y., Koenig,S., Nait-Oumesmar,B., Martin,D.M.,
            Wu,L.N., Xin,M., Zhou,W., Parras,C. and Lu,Q.R.
  TITLE     Dual Requirement of CHD8 for Chromatin Landscape Establishment and
            Histone Methyltransferase Recruitment to Promote CNS Myelination
            and Repair
  JOURNAL   Dev Cell 45 (6), 753-768 (2018)
   PUBMED   29920279
  REMARK    GeneRIF: Data indicate that CHD8 exhibits a dual function through
            inducing a cascade of chromatin reprogramming and recruiting H3K4
            histone methyltransferases to establish oligodendrocyte identity.
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   Katayama,Y., Nishiyama,M., Shoji,H., Ohkawa,Y., Kawamura,A.,
            Sato,T., Suyama,M., Takumi,T., Miyakawa,T. and Nakayama,K.I.
  TITLE     CHD8 haploinsufficiency results in autistic-like phenotypes in mice
  JOURNAL   Nature 537 (7622), 675-679 (2016)
   PUBMED   27602517
REFERENCE   3  (bases 1 to 8400)
  AUTHORS   Sugathan,A., Biagioli,M., Golzio,C., Erdin,S., Blumenthal,I.,
            Manavalan,P., Ragavendran,A., Brand,H., Lucente,D., Miles,J.,
            Sheridan,S.D., Stortchevoi,A., Kellis,M., Haggarty,S.J.,
            Katsanis,N., Gusella,J.F. and Talkowski,M.E.
  TITLE     CHD8 regulates neurodevelopmental pathways associated with autism
            spectrum disorder in neural progenitors
  JOURNAL   Proc Natl Acad Sci U S A 111 (42), E4468-E4477 (2014)
   PUBMED   25294932
REFERENCE   4  (bases 1 to 8400)
  AUTHORS   Bernier,R., Golzio,C., Xiong,B., Stessman,H.A., Coe,B.P., Penn,O.,
            Witherspoon,K., Gerdts,J., Baker,C., Vulto-van Silfhout,A.T.,
            Schuurs-Hoeijmakers,J.H., Fichera,M., Bosco,P., Buono,S.,
            Alberti,A., Failla,P., Peeters,H., Steyaert,J., Vissers,L.E.L.M.,
            Francescatto,L., Mefford,H.C., Rosenfeld,J.A., Bakken,T.,
            O'Roak,B.J., Pawlus,M., Moon,R., Shendure,J., Amaral,D.G., Lein,E.,
            Rankin,J., Romano,C., de Vries,B.B.A., Katsanis,N. and Eichler,E.E.
  TITLE     Disruptive CHD8 mutations define a subtype of autism early in
            development
  JOURNAL   Cell 158 (2), 263-276 (2014)
   PUBMED   24998929
REFERENCE   5  (bases 1 to 8400)
  AUTHORS   Shanks,M.O., Lund,L.M., Manni,S., Russell,M., Mauban,J.R. and
            Bond,M.
  TITLE     Chromodomain helicase binding protein 8 (Chd8) is a novel A-kinase
            anchoring protein expressed during rat cardiac development
  JOURNAL   PLoS One 7 (10), e46316 (2012)
   PUBMED   23071553
  REMARK    GeneRIF: These data add Chd8 to the short list of known nuclear
            AKAPs, and implicate a function for Chd8 in post-natal rat cardiac
            development
REFERENCE   6  (bases 1 to 8400)
  AUTHORS   Yamashina,K., Yamamoto,H., Chayama,K., Nakajima,K. and Kikuchi,A.
  TITLE     Suppression of STAT3 activity by Duplin, which is a negative
            regulator of the Wnt signal
  JOURNAL   J Biochem 139 (2), 305-314 (2006)
   PUBMED   16452319
REFERENCE   7  (bases 1 to 8400)
  AUTHORS   Dou,Y., Milne,T.A., Tackett,A.J., Smith,E.R., Fukuda,A.,
            Wysocka,J., Allis,C.D., Chait,B.T., Hess,J.L. and Roeder,R.G.
  TITLE     Physical association and coordinate function of the H3 K4
            methyltransferase MLL1 and the H4 K16 acetyltransferase MOF
  JOURNAL   Cell 121 (6), 873-885 (2005)
   PUBMED   15960975
REFERENCE   8  (bases 1 to 8400)
  AUTHORS   Nishiyama,M., Nakayama,K., Tsunematsu,R., Tsukiyama,T., Kikuchi,A.
            and Nakayama,K.I.
  TITLE     Early embryonic death in mice lacking the beta-catenin-binding
            protein Duplin
  JOURNAL   Mol Cell Biol 24 (19), 8386-8394 (2004)
   PUBMED   15367660
REFERENCE   9  (bases 1 to 8400)
  AUTHORS   Kobayashi,M., Kishida,S., Fukui,A., Michiue,T., Miyamoto,Y.,
            Okamoto,T., Yoneda,Y., Asashima,M. and Kikuchi,A.
  TITLE     Nuclear localization of Duplin, a beta-catenin-binding protein, is
            essential for its inhibitory activity on the Wnt signaling pathway
  JOURNAL   J Biol Chem 277 (8), 5816-5822 (2002)
   PUBMED   11744694
REFERENCE   10 (bases 1 to 8400)
  AUTHORS   Sakamoto,I., Kishida,S., Fukui,A., Kishida,M., Yamamoto,H.,
            Hino,S., Michiue,T., Takada,S., Asashima,M. and Kikuchi,A.
  TITLE     A novel beta-catenin-binding protein inhibits
            beta-catenin-dependent Tcf activation and axis formation
  JOURNAL   J Biol Chem 275 (42), 32871-32878 (2000)
   PUBMED   10921920
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1 and AF169825.1.
            
            Summary: This gene encodes a member of the
            chromodomain-helicase-DNA binding protein family, which is
            characterized by a SNF2-like domain and two chromatin organization
            modifier domains. The encoded protein also contains brahma and
            kismet domains, which is common to the subfamily of
            chromodomain-helicase-DNA binding proteins to which this protein
            belongs. In mammals, this gene has been shown to function in
            several processes including transcriptional regulation, epigenetic
            remodeling, promotion of cell proliferation, and regulation of RNA
            synthesis. Knockout of this gene in mice causes early embryonic
            lethality due to widespread apoptosis. Heterozygous loss of
            function mutations in mice result in autism spectrum disorder-like
            behaviors that include increased anxiety, repetitive behavior, and
            altered social behavior. [provided by RefSeq, Dec 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.53671.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-215               JAXUCZ010000015.1  27417637-27417851   c
            216-2441            AF169825.1         1-2226
            2442-2579           JAXUCZ010000015.1  27403697-27403834   c
            2580-2701           JAXUCZ010000015.1  27402516-27402637   c
            2702-2945           JAXUCZ010000015.1  27402126-27402369   c
            2946-3122           JAXUCZ010000015.1  27400852-27401028   c
            3123-3266           JAXUCZ010000015.1  27399722-27399865   c
            3267-3522           JAXUCZ010000015.1  27399268-27399523   c
            3523-3733           JAXUCZ010000015.1  27397525-27397735   c
            3734-3929           JAXUCZ010000015.1  27397176-27397371   c
            3930-4097           JAXUCZ010000015.1  27396732-27396899   c
            4098-4277           JAXUCZ010000015.1  27396205-27396384   c
            4278-4388           JAXUCZ010000015.1  27395951-27396061   c
            4389-4585           JAXUCZ010000015.1  27394984-27395180   c
            4586-4785           JAXUCZ010000015.1  27394541-27394740   c
            4786-4942           JAXUCZ010000015.1  27394280-27394436   c
            4943-5032           JAXUCZ010000015.1  27394114-27394203   c
            5033-5136           JAXUCZ010000015.1  27393497-27393600   c
            5137-5266           JAXUCZ010000015.1  27392144-27392273   c
            5267-5342           JAXUCZ010000015.1  27389521-27389596   c
            5343-5397           JAXUCZ010000015.1  27388767-27388821   c
            5398-5605           JAXUCZ010000015.1  27388395-27388602   c
            5606-5814           JAXUCZ010000015.1  27386953-27387161   c
            5815-6531           JAXUCZ010000015.1  27386150-27386866   c
            6532-6683           JAXUCZ010000015.1  27385787-27385938   c
            6684-6986           JAXUCZ010000015.1  27385212-27385514   c
            6987-7100           JAXUCZ010000015.1  27383867-27383980   c
            7101-7280           JAXUCZ010000015.1  27383487-27383666   c
            7281-7397           JAXUCZ010000015.1  27383011-27383127   c
            7398-8400           JAXUCZ010000015.1  27379285-27380287   c
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p14"
     gene            1..8400
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /note="chromodomain helicase DNA binding protein 8"
                     /db_xref="GeneID:65027"
                     /db_xref="RGD:620696"
     exon            1..1058
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..7961
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /EC_number="3.6.4.12"
                     /note="duplin; axis duplication inhibitor; ATP-dependent
                     helicase CHD8; beta-catenin binding protein"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 8"
                     /protein_id="NP_001334590.1"
                     /db_xref="GeneID:65027"
                     /db_xref="RGD:620696"
                     /translation="MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLD
                     SLDQMNQDGGSGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQ
                     EQPAQPVLQTSTPTSGLLQVSKSQEILSQGNPFMGVSATAVSPSNTGGQPSQSAPKIV
                     ILKAPPNSSVTGAHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIV
                     SGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGATGPPLKPAVTLT
                     STPAQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKSAQGQP
                     AKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQ
                     RLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEENR
                     RLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSSG
                     ERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPRED
                     EESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVPEPDGE
                     TLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEW
                     ATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDN
                     GEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELKRVNRPQANAWKK
                     LELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNV
                     GIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLI
                     PGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHK
                     VLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMML
                     RRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTM
                     MELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPKLK
                     AGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFV
                     FLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNS
                     YEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDD
                     EGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWA
                     KKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHD
                     RHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHY
                     RGDENIKSFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWI
                     RKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDI
                     WFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAI
                     AAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGD
                     EAPVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKIEAAERGDRRRRRCEAAF
                     KLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTDES
                     LTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQV
                     LCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFL
                     AARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDVPAEKSPEENAVQV
                     PSLDSLTLKLEDEVVARSRLTPQDYEIRVASSDTAPLSRSVPPVKLEDDDDSDSELDL
                     SKLSPSSSSSSSSSSSSSSSDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPN
                     EDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEMTTGGI
                     LGPGNHLLDSPSLTPGEYGDSPVPTPRSSSAASMVEEEASAVTTAAAQFTKLRRGMDE
                     KEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNH
                     LDVDLETRIPVINKVDGTLLVGDEAPRRAELDMWLQGHPEFAVDPRFLAYMEERRKQK
                     WQRCKKNNKTELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPIAPENSKKRVRRT
                     RPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSATSASLPFMPFVMGGA
                     AAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQHED
                     DDEEEDEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSDDADD"
     exon            1059..1430
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1816
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1931
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1932..2114
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2183
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2239
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2240..2357
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2441
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2579
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2580..2701
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..2945
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2946..3122
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            3123..3266
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3522
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            3523..3733
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            3734..3929
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            3930..4097
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            4098..4277
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            4278..4388
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            4389..4585
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            4586..4785
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            4786..4942
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            4943..5032
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            5033..5136
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            5137..5266
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            5267..5342
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            5343..5397
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            5398..5605
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            5606..5814
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            5815..6531
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            6532..6683
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            6684..6986
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            6987..7100
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            7101..7280
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            7281..7397
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
     exon            7398..8400
                     /gene="Chd8"
                     /gene_synonym="CHD-8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagttccatc ttctccctct gaagacttag ctgtcttgct ccatgaagtt caggacaatc
       61 tgacatgccc ttgattcttg tctcttcatt tgaagaatag tcctcatcca ttgtgtacat
      121 ttttttgtta atagggaagg ggaggggaga aagagttccc cccctcccct tccctcccca
      181 gaacttcaac aaatatcctt tattacttta ccaagatggc agaccccatc atggatctgt
      241 ttgatgaccc aaacttattt ggcctggatt ctttgactga tgacagcttt aatcaggtca
      301 ctcaagaccc cattgaagaa gcacttggac tgccaagttc tctggactcc ttggatcaga
      361 tgaaccaaga tggtggaagt ggtgatgtgg ggaattcatc agcaagtgac ctggttcccc
      421 caccagagga aacagcttcc actgaacttc ccaaagaatc cacagctcca gctccagaat
      481 ccttaacctt gcatgattat accactcagc ccaccagcca ggagcagcca gcccaacctg
      541 tcttgcagac atcaacacca acatcaggac ttttgcaggt ctctaagagc caggagatcc
      601 tgagccaagg gaatcctttc atgggtgtct ctgccacagc tgtctccccc agtaatactg
      661 gaggacagcc atctcagtca gctcctaaga ttgttattct taaagctcca ccaaactcct
      721 cagtcacagg tgcccatgtg gcacaaattc aggcccaagg tatcaccagt acagctcagc
      781 ccctagtggc tggcacagcc aatggtggaa aagtcacttt taccaaagtg cttactggta
      841 cacccctccg accaggtgta tccattgtct ctggtaatac agtgctggcc acgaaggtcc
      901 ctgggaacca ggctgctgtt cagcgcattg tccagccaag ccgaccagta aagcagctag
      961 ttctccagcc agttaagggt tccgctcctg ctggaaatcc cggggctacc gggccccccc
     1021 tgaagcctgc agttacactg acatctacac ctgcccaggg tgaatcgaaa cgcatcactt
     1081 tggtccttca gcagccacag tccggaggtc cccaaggaca tcggcatgtt gtgttaggga
     1141 gtctacccgg caagatagtg ttacagggca accaactggc agccttgact caagccaaga
     1201 gtgctcaggg gcagcctgcc aaagtagtca ccatccaact gcaggtgcag cagccacagc
     1261 aaaagatcca gattgtacca cagcccccat catcacagcc acagccacag ccgccaccct
     1321 cagcccaacc attgaccctg tcctctgtac agcaggctca gataatggga ccaggccaaa
     1381 acccaggaca gagactttct gtaccgctca agatggtgct gcagccacag gctggctctt
     1441 ctcagggagc ctcttctgga ctctctgtgg ttaaagttct aagtgccagt gaagtggcag
     1501 ccctgtcatc accagcaagc tgtgcccctc acactgcagg gaagactgga atggaggaga
     1561 accgcaggct tgagcaccag aaaaagcaag aaaaagcaaa tcggattgtg gcagaggcca
     1621 ttgctagggc ccgggccagg ggtgagcaaa acatacctcg agtcctgaat gaggatgagc
     1681 tgcctagtgt ccggccagaa gaggagggtg aaaagaaacg caggaagaag agcagtggag
     1741 aaaggctgaa ggaagagaag ccaaagaaaa gcaagactgc tgctgcctcc aaaacaaagg
     1801 gcaagagtaa gctaaacacc atcactcctg tagtgggcaa aaagagaaag cgtaacacat
     1861 cgtctgacaa ctctgatgtg gaggtcatgc ctgcacagtc gccccgggag gacgaagaga
     1921 gcagcatcca gaagagacgt tcaaaccgcc aagttaagcg aaaaaaatat acagaggacc
     1981 tggatataaa gataacagat gatgaagagg aggaggaggt cgatgttact ggtccaataa
     2041 aacccgagcc catcctccca gaaccagtgc cagagcctga tggggagact ctgccttcca
     2101 tgcagttctt tgtggagaat cccagtgaag aagacgcagc tattgtggac aaagtgcttt
     2161 ccatgcgagt tgtgaagaaa gagcttcctt ctggacagta cactgaggct gaggaattct
     2221 ttgtcaagta caagaactac tcctatctgc actgtgaatg ggcaactatc tcccagctgg
     2281 agaaggacaa gaggatccat cagaagctaa agcgcttcaa gacgaaaatg gctcagatga
     2341 ggcacttctt tcatgaagat gaagagcctt tcaatccaga ctacgtcgag gtggacagga
     2401 tactggacga gtctcacagt gtggacaagg ataatggtga gcctgtaatc tactacctgg
     2461 taaaatggtg ctctctgccc tatgaggaca gtacgtggga gctaaaagag gatgttgacg
     2521 agggcaagat tcgggaattt aaacggatcc agtcaaggca tccagaactc aaaagggtga
     2581 atcgtccaca ggcaaatgcc tggaagaaat tggagttgtc acatgaatat aaaaacagaa
     2641 accaattacg ggagtatcag ttagaagggg tcaactggct tctctttaat tggtataaca
     2701 ggcagaactg catcctggct gatgaaatgg gattaggcaa aaccattcag tccattgcct
     2761 tcttgcagga ggtatataat gtgggcatcc atggtccctt tttggtcatt gctccattgt
     2821 ccaccattac taactgggag cgagaattta atacctggac agagatgaac actattgtgt
     2881 accatggcag cctggccagc cggcagatga ttcagcaata tgaaatgtac tgtaaagatt
     2941 cacggggtcg cctcatccca ggtgcatata agtttgatgc cttgatcacc acttttgaga
     3001 tgattttgtc agactgtcct gaacttcgtg aaattgaatg gcgttgtgtt atcattgatg
     3061 aagcccatcg gctaaagaac cgtaattgta agctactcga tagcctcaag cacatggacc
     3121 tggagcataa agtgttactt acaggaacac cattgcaaaa tactgtagag gagctgttca
     3181 gcctacttca tttcttggaa ccttctcagt ttccctcaga atcagaattc cttaaggatt
     3241 ttggggatct gaagacagag gagcaggttc aaaagcttca ggccattcta aagccaatga
     3301 tgctgagaag actcaaagag gatgttgaaa agaatttggc tcccaaacag gagactatta
     3361 ttgaagtaga gctgaccaac atccagaaga aatactacag agctatttta gagaagaatt
     3421 tctccttcct ttccaaagga gcagggcata ccaatatgcc taacctcctg aacacgatga
     3481 tggagttacg caagtgctgc aaccacccat acctcatcaa tggtgcagaa gagaaaatcc
     3541 tgatggaatt tcgggaagct tgccatatta tacctcaaga tttccatctg caggccatgg
     3601 ttcggtcagc tggcaaatta gttcttattg acaagttact tccaaagctt aaagctggtg
     3661 gccataaagt tctgatcttc tcccagatgg tacgctgtct agatattctg gaggattatc
     3721 tgatccagag gaggtactta tacgaacgca ttgatgggcg agttagaggc aaccttcgac
     3781 aagctgctat cgaccgcttt agcaagcctg actcagaccg ctttgtcttc ttgctatgta
     3841 cccgtgctgg tggacttggt attaatctta cagctgctga tacctgtatt atttttgatt
     3901 cagactggaa tccacagaat gacctgcagg cccaagcacg ttgtcatcgc attggacaga
     3961 gcaaagctgt aaaggtgtac cgactcatca ctcgcaattc ctacgagaga gagatgtttg
     4021 ataaagctag cctcaagttg gggctggaca aagctgtgct tcagtccatg agtggtcggg
     4081 atggcaacat tactggaatt caacagttct ccaagaaaga gattgaagat ctcttaagga
     4141 aaggtgcata tgcagccata atggaggaag acgatgaggg ttctaagttt tgtgaagagg
     4201 acatagatca gatcttgctg agacggacta caaccatcac tattgagtct gaagggaaag
     4261 ggtctacttt tgccaaggca agctttgttg cttctgaaaa taggacagat atttccctgg
     4321 atgatccaaa cttttggcaa aaatgggcca aaaaggcaga cctagacatg gatctgctga
     4381 atagtaagaa taacttagtg attgatacac cacgagttcg gaaacagact cgacacttca
     4441 gcactctaaa agatgacgac ctggttgagt tttctgattt agaaagtgaa gatgatgaac
     4501 gtccccgttc ccgaagacat gaccgtcatc acacctatgg gcgcactgac tgctttcggg
     4561 tagaaaaaca tctcctggta tatggttggg gacgttggag agatattctg tctcatggac
     4621 gatttaagcg gcgcatgact gaaagagatg tggagacaat atgccgagcc atccttgtgt
     4681 actgtcttct acactaccgt ggggatgaaa acatcaaaag cttcatttgg gacttgatta
     4741 gccctgctga aaatggcaag acaaaagaat tgcagaatca ttcaggtctg tctatccctg
     4801 tgccccgtgg gcgtaagggg aaaaaagtga agtcacaaag tacttttgac attcataagg
     4861 cagactggat ccggaaatat aaccctgata ctctgttcca agacgagagc tataagaagc
     4921 acttgaaaca tcagtgtaac aaggtactgt tgagagtacg gatgctgtac tacctgagac
     4981 aggaggtcat tggagaccaa gcagagaagg tgttaggggg cgccatagcc agtgagattg
     5041 acatatggtt cccagtagtg gatcagctgg aggttccaac aacatggtgg gatagtgagg
     5101 ctgacaaatc cctgctcatt ggcgttttta agcatggcta tgaaaaatac aataccatga
     5161 gggcagaccc tgccttgtgt ttcctggaaa aagctggcag gccagatgac aaagccattg
     5221 cagcggagca cagagttctg gataacttct ccgacctggt agaagggatt gactttgata
     5281 aggattgtga agatcctgaa tataaacccc ttcagggtcc cccaaaggac ccggatgatg
     5341 agggtgatcc cttgatgatg atggatgagg agatctcagt cattgacgga gatgaagccc
     5401 cggtgactca acagccaggg catttattct ggcctccagg ctctgccctc acagctaggc
     5461 tccgccgcct ggtaaccgcc tatcaacgca gctacaagag agaacaaatg aagatagagg
     5521 ctgcggagcg tggggaccgg agacgccgac gttgtgaagc ggcctttaag ctcaaagaaa
     5581 ttgcaaggcg ggaaaaacaa caacgatgga cacggcgcga gcaaactgat ttctatcgag
     5641 tagtgtctac ctttggggta gagtacgacc ctgacaacat gcagtttcac tgggatcgct
     5701 tccgtacttt tgctcgcctg gacaaaaaaa cagatgaaag ccttaccaag tacttccatg
     5761 gttttgtggc catgtgtaga caagtgtgcc gccttccccc agctgctggt gatgaacctc
     5821 cagatcctaa tctgtttatt gagcccatca ctgaagagag agcatcacgg actctctacc
     5881 gaatcgagtt gcttcgacgc ttacgggaac aagttttatg ccaccccctt ttagaagatc
     5941 ggctggcatt gtgtcagcct ccaggtcttg aattgcccaa atggtgggaa cctgtccgtc
     6001 acgatgggga gctcctccga ggagcagccc gccatggggt gagccaaaca gactgcaaca
     6061 tcatgcagga cccagacttc tcttttctgg ctgcccgtat gaattatatg cagaaccatc
     6121 aggcaggagc atcagctgca tccctgtcac gatgctccac tccactgctg caccagcagt
     6181 gtacctcacg cactgcttca ccatcacccc tgcgcccaga tgttcctgct gaaaagtcac
     6241 ctgaggagaa tgctgttcag gtccccagtc tggacagtct gactttaaaa ctagaggatg
     6301 aggtggtggc taggagccga ctcaccccac aagactatga aattcgtgta gcctcttcag
     6361 atacagcccc tctgtcccgg agtgttccgc cagtcaaact ggaggatgac gatgattcag
     6421 actctgagct ggacttgagc aagttgtcac catcgtcctc ttcatcttca tcctcctcca
     6481 gctccagctc cagcagtgat gagagtgaag atgagaagga agagaagtta actgctgacc
     6541 ggtcccgccc aaagctctat gatgaagaga gccttctgtc ccttactatg tcccaagatg
     6601 gattcccaaa tgaagatgga gaacaaatga cccctgagtt gctgctgctg caggaaagac
     6661 aaagagcctc tgaatggccc aaggatcgtg ttctgataaa ccgcattgac ctcgtctgcc
     6721 aggctgtact ctcagggaaa tggccttcta accgccggag ccaggaaatg acaacaggag
     6781 gaattttggg gccaggaaac catttattag acagtccctc tttgacccca ggagaatatg
     6841 gggactctcc ggtccccaca ccacgaagca gcagtgcagc ttccatggta gaggaagaag
     6901 catctgcggt caccacagca gcagcccaat ttactaaact tcggcgaggc atggacgaga
     6961 aagagtttac agttcagatc aaagatgaag aaggattgaa gttaacattc cagaagcata
     7021 gattgatggc taatggtgta atgggagatg gacatccact gtttcataag aagaagggga
     7081 acaggaaaaa gctagtagag ctggaggtgg agtgcatgga agagcctaat caccttgatg
     7141 tggacctgga gacccggatc cctgtcatca ataaggtgga tggtactttg ctggtgggtg
     7201 atgaggcccc tcgccgggcg gagctggaca tgtggttaca gggtcatcca gagtttgctg
     7261 ttgatccccg atttctagcg tatatggagg aacgtagaaa acagaagtgg cagagatgta
     7321 aaaaaaataa taagacagaa ttgaactgtt tgggaatgga accagtccag ccagccaact
     7381 ctagaaatgg gaaaaagggt cactatgctg aaactgcgtt caaccgggtt ttgccagggc
     7441 ctattgcacc agaaaacagc aagaaacggg tccgcaggac aagaccagac ctttctaaga
     7501 tgatggccct gatgcagggt ggaagcactg ggtccctatc tctgcataac acctttcaac
     7561 acagcagtag taacctacag tctgtgtcgt ctctgggtca cagcagtgcc acttctgcat
     7621 ctctgccttt catgccgttt gtgatgggtg gtgcagcagc accccctcat gtagactcca
     7681 gcaccatgct tcatcatcac caccaccacc cccaccccca ccatcaccac catcaccatc
     7741 caggcttgag aaccactggc tacccttctt caccagctac taccacctct ggtactgcct
     7801 tgcggttacc aacactgcag catgaggacg atgatgaaga ggaggatgaa gatgatgatg
     7861 atttatctca gggctatgat agctcagaaa gggacttctc actcattgat gaccctatga
     7921 tgccagccaa ctcagactcc agtgatgatg ctgacgactg aagccctaat atagacccgt
     7981 gctgggtggc tgctggaatc ccttactctg gcccttggac tttggaaatg tgggaggggc
     8041 agggaacaat atggggaaac ccaagacttc aggaggtgaa aaggaaaaga aaaaaaaaag
     8101 gaaaaaaaaa aaaacaaaaa actcgtaccc gattgctccc agttttgaga ggattgggtg
     8161 ggcaggggaa ctcctaaaaa ataataaatg accacttcct cgtttctggg ggagcaaagg
     8221 agcttagagc agctggtgtg ctcacccctc cccagacacc tccattaacc acagactatg
     8281 tagcgctggc cctagcctct ggcagagcct gttcctggcc aaactgtgga tacagctgga
     8341 gggtcaggga ctgttacctt ccttcccctt ggcattaata aatttaagtt aatccttttc
//
