LOCUS       NM_001170593            3599 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Rattus norvegicus choline O-acetyltransferase (Chat), mRNA.
ACCESSION   NM_001170593
VERSION     NM_001170593.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Crews,F.T., Fisher,R.P., Qin,L. and Vetreno,R.P.
  TITLE     HMGB1 neuroimmune signaling and REST-G9a gene repression contribute
            to ethanol-induced reversible suppression of the cholinergic neuron
            phenotype
  JOURNAL   Mol Psychiatry 28 (12), 5159-5172 (2023)
   PUBMED   37402853
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Corsetti,V., Perrone-Capano,C., Salazar Intriago,M.S.,
            Botticelli,E., Poiana,G., Augusti-Tocco,G., Biagioni,S. and
            Tata,A.M.
  TITLE     Expression of Cholinergic Markers and Characterization of Splice
            Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons
  JOURNAL   Int J Mol Sci 22 (11), 5499 (2021)
   PUBMED   34071104
  REMARK    GeneRIF: Expression of Cholinergic Markers and Characterization of
            Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia
            Neurons.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3599)
  AUTHORS   Linker,L.A., Carlson,L., Godfrey,D.A., Parli,J.A. and Ross,C.D.
  TITLE     Quantitative distribution of choline acetyltransferase activity in
            rat trapezoid body
  JOURNAL   Hear Res 370, 264-271 (2018)
   PUBMED   30177425
  REMARK    GeneRIF: Higher choline acetyltransferase activity rostrally than
            caudally in the trapezoid body is consistent with evidence that the
            centrifugal cholinergic innervation of the cochlear nucleus derives
            predominantly from locations at or rostral to its anterior part, in
            the superior olivary complex and pontomesencephalic tegmentum.
REFERENCE   4  (bases 1 to 3599)
  AUTHORS   Han,S., Yang,S.H., Kim,J.Y., Mo,S., Yang,E., Song,K.M., Ham,B.J.,
            Mechawar,N., Turecki,G., Lee,H.W. and Kim,H.
  TITLE     Down-regulation of cholinergic signaling in the habenula induces
            anhedonia-like behavior
  JOURNAL   Sci Rep 7 (1), 900 (2017)
   PUBMED   28420875
  REMARK    Erratum:[Sci Rep. 2017 Dec 1;7(1):17090. doi:
            10.1038/s41598-017-16991-1.. PMID: 29196669]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3599)
  AUTHORS   Soligo,M., Protto,V., Florenzano,F., Bracci-Laudiero,L., De
            Benedetti,F., Chiaretti,A. and Manni,L.
  TITLE     The mature/pro nerve growth factor ratio is decreased in the brain
            of diabetic rats: Analysis by ELISA methods
  JOURNAL   Brain Res 1624, 455-468 (2015)
   PUBMED   26282349
REFERENCE   6  (bases 1 to 3599)
  AUTHORS   Ishii,K., Oda,Y., Ichikawa,T. and Deguchi,T.
  TITLE     Complementary DNAs for choline acetyltransferase from spinal cords
            of rat and mouse: nucleotide sequences, expression in mammalian
            cells, and in situ hybridization
  JOURNAL   Brain Res Mol Brain Res 7 (2), 151-159 (1990)
   PUBMED   2160042
REFERENCE   7  (bases 1 to 3599)
  AUTHORS   Ross,C.D. and Godfrey,D.A.
  TITLE     Distributions of choline acetyltransferase and acetylcholinesterase
            activities in layers of rat superior colliculus
  JOURNAL   J Histochem Cytochem 33 (7), 631-641 (1985)
   PUBMED   4008917
REFERENCE   8  (bases 1 to 3599)
  AUTHORS   Benishin,C.G. and Carroll,P.T.
  TITLE     Multiple forms of choline-O-acetyltransferase in mouse and rat
            brain: solubilization and characterization
  JOURNAL   J Neurochem 41 (4), 1030-1039 (1983)
   PUBMED   6619842
REFERENCE   9  (bases 1 to 3599)
  AUTHORS   Packman,P.M., Godfrey,D.A., Williams,A.D. and Matschinsky,F.M.
  TITLE     Quantitative histochemical studies of the hypothalamus enzymes of
            acetylcholine metabolism
  JOURNAL   J Histochem Cytochem 25 (11), 1237-1246 (1977)
   PUBMED   334962
REFERENCE   10 (bases 1 to 3599)
  AUTHORS   Godfrey,D.A. and Matschinsky,F.M.
  TITLE     Enzymes of the cholinergic system in islets of Langerhans
  JOURNAL   J Histochem Cytochem 23 (9), 645-651 (1975)
   PUBMED   126256
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000016.1 and CB605690.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN16418971 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-102               JAXUCZ010000016.1  7723315-7723416     c
            103-293             JAXUCZ010000016.1  7718196-7718386     c
            294-394             JAXUCZ010000016.1  7716138-7716238     c
            395-586             JAXUCZ010000016.1  7712006-7712197     c
            587-705             JAXUCZ010000016.1  7710613-7710731     c
            706-759             JAXUCZ010000016.1  7709343-7709396     c
            760-940             JAXUCZ010000016.1  7704152-7704332     c
            941-1118            JAXUCZ010000016.1  7702012-7702189     c
            1119-1288           JAXUCZ010000016.1  7681837-7682006     c
            1289-1389           JAXUCZ010000016.1  7679557-7679657     c
            1390-1518           JAXUCZ010000016.1  7678690-7678818     c
            1519-1545           JAXUCZ010000016.1  7675983-7676009     c
            1546-1687           CB605690.1         157-298
            1688-1783           JAXUCZ010000016.1  7674482-7674577     c
            1784-1846           JAXUCZ010000016.1  7674283-7674345     c
            1847-1984           JAXUCZ010000016.1  7667532-7667669     c
            1985-3599           JAXUCZ010000016.1  7663665-7665279     c
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p16"
     gene            1..3599
                     /gene="Chat"
                     /note="choline O-acetyltransferase"
                     /db_xref="GeneID:290567"
                     /db_xref="RGD:1304627"
     exon            1..102
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            103..293
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            294..394
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     CDS             332..2266
                     /gene="Chat"
                     /EC_number="2.3.1.6"
                     /note="CHOACTase; choline acetylase; choline
                     acetyltransferase"
                     /codon_start=1
                     /product="choline O-acetyltransferase"
                     /protein_id="NP_001164064.1"
                     /db_xref="GeneID:290567"
                     /db_xref="RGD:1304627"
                     /translation="MPILEKAPQKMPVKASSWEELDLPKLPVPPLQQTLATYLQCMQH
                     LVPEEQFRKSQAIVKRFGAPGGLGETLQEKLLERQEKTANWVSEYWLNDMYLNNRLAL
                     PVNSSPAVIFARQHFQDTNDQLRFAACLISGVLSYKTLLDSHSLPTDWAKGQLSGQPL
                     CMKQYYRLFSSYRLPGHTQDTLVAQKSSIMPEPEHVIVACCNQFFVLDVVINFRRLSE
                     GDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAKARTVLLKDSTNRDSLDMIERC
                     ICLVCLDGPGTGELSDTHRALQLLHGGGCSLNGANRWYDKSLQFVVGRDGTCGVVCEH
                     SPFDGIVLVQCTEHLLKHMMTSNKKLVRADSVSELPAPRRLRWKCSPETQGHLASSAE
                     KLQRIVKNLDFIVYKFDNYGKTFIKKQKYSPDGFIQVALQLAYYRLYQRLVPTYESAS
                     IRRFQEGRVDNIRSATPEALAFVQAMTDHKAAMPASEKLQLLQTAMQAQTEYTVMAIT
                     GMAIDNHLLALRELARDLCKEPPEMFMDETYLMSNRFVLSTSQVPTTMEMFCCYGPVV
                     PNGYGACYNPQPEAITFCISSFHSCKETSSVEFAEAVGASLVDMRDLCSSRQPADSKP
                     PAPKEKARGPTKPSNLDSSH"
     exon            395..586
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            587..705
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            706..759
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            760..940
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1118
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1288
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1389
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1518
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1641
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1783
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1846
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1984
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..3599
                     /gene="Chat"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgtgcagcac ccccggaagg aaggtgagcc ttcctaagcc tctactgaca gcaaagctgc
       61 agaggccctg ccgcgtgaga cccagaagct tccaagccac tttcagtcag tcggggcggc
      121 tgctgggacc tgacaacgtc gtcgtctgag gctctactac agaacctagg cggcggcccc
      181 aacctctgac actgctgcca cccctccttg atccttctgg ctcacagaac cgcctccagc
      241 cctgcttggt gtacagcagc gctggttcgg cacggaacag ccctggagag caggtctaca
      301 gctctgctac tctggagcga gagtcactac aatgcccatc ctggaaaagg ctccccaaaa
      361 gatgcctgta aaggcttcta gctgggagga gctggactta cctaaattac cagtgccccc
      421 gctgcagcaa accctggcca cctacctaca gtgcatgcag catctggtac ctgaagagca
      481 gttcaggaag agccaggcca ttgtgaagcg gtttggggcc cctggtggcc tgggtgagac
      541 cctgcaggaa aagctcttgg agagacagga gaagacagcc aattgggtct ctgaatactg
      601 gctgaatgac atgtacctga acaaccgcct ggccctgcca gtcaactcca gccctgctgt
      661 gatctttgct cggcagcact tccaagacac caatgaccag ctaagatttg cagcctgcct
      721 catctctggt gtgcttagct acaaaactct gctggacagc cactcccttc ccactgactg
      781 ggccaagggg cagctctcag ggcagcccct ctgtatgaag caatattaca gactcttctc
      841 ctcttaccgg cttcctggcc atacccagga cacactggtg gcccagaaga gcagtatcat
      901 gcccgagcct gagcatgtca tcgtggcctg ctgcaaccag ttctttgtct tggatgttgt
      961 cattaatttc cgccgtctca gtgagggtga tctgttcact cagttgagaa agatagtcaa
     1021 aatggcgtcc aacgaggatg aacgcctgcc tccaatcggc ctgctgacgt cagacgggag
     1081 gagcgagtgg gccaaggcca ggacggtcct cttaaaagac tccaccaacc gggactccct
     1141 ggacatgatc gagcgctgca tctgcctggt atgcctggat ggtccaggca caggagagct
     1201 cagtgacacc cacagggccc tccaactcct tcacggtgga ggctgcagcc tgaacggagc
     1261 caatcgctgg tatgacaagt ccctgcagtt cgtggtgggc cgagatggca cctgcggtgt
     1321 ggtgtgtgag cattctccat ttgatggcat cgtcctggtg cagtgcacag agcacctgct
     1381 gaaacatatg atgacaagca acaagaagct cgtcagggct gactcagtga gtgagctccc
     1441 tgcccccaga aggctgaggt ggaaatgttc cccagaaacc caaggccatc tcgcctcctc
     1501 agcagagaaa cttcaaagaa tagtaaagaa tctggatttc attgtttaca agtttgacaa
     1561 ctatggaaaa acatttatca agaagcagaa atacagcccc gacggcttca tccaggtggc
     1621 cctccagctg gcttactaca ggctttacca gagactggtg cccacctatg agagcgcatc
     1681 catccgccgc ttccaggaag gtcgggtgga caacatcaga tcagccactc cagaggctct
     1741 ggcttttgtg caagccatga ctgaccacaa ggctgccatg ccggcttctg agaaactgca
     1801 gctgctgcag acagccatgc aggcccagac tgagtacaca gtcatggcca taaccggcat
     1861 ggccattgac aaccatcttc tggcactgag ggagctggcc cgagacctgt gcaaagagcc
     1921 acctgagatg ttcatggatg aaacatacct gatgagcaac cggtttgtcc tctccaccag
     1981 ccaggtgccc acaaccatgg agatgttctg ctgttatgga cccgtggtcc ccaatggcta
     2041 tggagcctgc tacaaccccc agcccgaggc catcaccttc tgcatctcca gttttcacag
     2101 ctgcaaagag acctcgtctg tggagtttgc ggaagctgtg ggagcgagcc ttgttgacat
     2161 gagagacctc tgcagttcaa ggcagcctgc tgacagcaag ccaccagcac ccaaggaaaa
     2221 agctaggggc ccaaccaagc caagcaatct tgactccagc cactagccca gcctcctaca
     2281 gaagtcagac cctactaagc tctgttccca tccccacccc agcttgctgc tgctccccct
     2341 aaccttgggg gctcacacga agctggcatg ttaagagaga gcccctcatt atatttatca
     2401 aggtggcctc tgtcctttac aactggaaat gagacccagc ctggtttgga agcaacctgg
     2461 gtgagctggg aattcccgat gaggtcttca gaaacgtaac ctttaccctt ttcccagcaa
     2521 cacccagtgg cacacatggt agctctgccc agtggaagaa cagtcatctc accacatgtt
     2581 atgggcccaa ataggtcatg aaggcagagg ctagctctgg atcactatct gtggctgtcc
     2641 ctccacacac tcacgtgcac acagatagcc tgagtgggtt tagaatacag tggccggcac
     2701 ccgcaaagac atttcttcct ttatcttcca tagcacagac ttcctggagt tccgataatc
     2761 tcatccagct attcagagag agagagagag agagagagag agagagagag agagaggagg
     2821 gagaaggaag gaaggaagga aggaaggaag gaaggaagga aggaaggaag accagcaatt
     2881 atcttcattt gatggataat ggagctgtct ctggcaagac tgcgagaact aaagctgggt
     2941 tgtgggtgaa ggggccacat ggctgacaag gagtggggcc ccctttggga agacactgtc
     3001 caaaggcgtt gcttgagtga ttccctccta aactactgat ctgagagaac caagccttgg
     3061 ttgctcttct ccatcagaaa agaaactcac tgtatttatt ggatgtatca aaccatgcac
     3121 tgtgagggtg taagacccat acctggcaga gccttggctg gagggaacac ctcctccctt
     3181 aggaagagac tggaagctgg ccttccccct ccaagaacaa aggagtgtac ttatatttat
     3241 gcaaaggcaa cactgagcca cggccacagg ctgctctgtg gggaccttgg gaaaggaggc
     3301 tattccactc ctacgtgtct ctcagagatg gaaaatgaca cagttgtttg aagcggtgac
     3361 ccattgtaag tatggccacc ttgtcatact gctaaattgc caaattattt atgaagttga
     3421 tgtttgggtt ctatttttat aatatagttc tagatttatg gcaatatgta catcttaatt
     3481 tatagttctg tgctttaaag aaatccatgt atgaataaag agcagaatat tgaaagcatt
     3541 ccattcaaga tatttaagga cctgtaagtc tgtattcaat aaaatgcaat gccaagaaa
//
