LOCUS       NM_001399362            6821 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus ceramide synthase 6 (Cers6), transcript variant
            1, mRNA.
ACCESSION   NM_001399362 XM_006234308
VERSION     NM_001399362.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Novgorodov,S.A., Riley,C.L., Keffler,J.A., Yu,J., Kindy,M.S.,
            Macklin,W.B., Lombard,D.B. and Gudz,T.I.
  TITLE     SIRT3 Deacetylates Ceramide Synthases: IMPLICATIONS FOR
            MITOCHONDRIAL DYSFUNCTION AND BRAIN INJURY
  JOURNAL   J Biol Chem 291 (4), 1957-1973 (2016)
   PUBMED   26620563
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Ebel,P., Vom Dorp,K., Petrasch-Parwez,E., Zlomuzica,A.,
            Kinugawa,K., Mariani,J., Minich,D., Ginkel,C., Welcker,J.,
            Degen,J., Eckhardt,M., Dere,E., Dormann,P. and Willecke,K.
  TITLE     Inactivation of ceramide synthase 6 in mice results in an altered
            sphingolipid metabolism and behavioral abnormalities
  JOURNAL   J Biol Chem 288 (29), 21433-21447 (2013)
   PUBMED   23760501
REFERENCE   3  (bases 1 to 6821)
  AUTHORS   Novgorodov,S.A., Chudakova,D.A., Wheeler,B.W., Bielawski,J.,
            Kindy,M.S., Obeid,L.M. and Gudz,T.I.
  TITLE     Developmentally regulated ceramide synthase 6 increases
            mitochondrial Ca2+ loading capacity and promotes apoptosis
  JOURNAL   J Biol Chem 286 (6), 4644-4658 (2011)
   PUBMED   21148554
  REMARK    GeneRIF: a novel role for CerS6 in the regulation of both
            mitochondrial Ca(2+) homeostasis and calpain, which appears to be
            important in OL apoptosis during brain development.
REFERENCE   4  (bases 1 to 6821)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   5  (bases 1 to 6821)
  AUTHORS   Lahiri,S., Lee,H., Mesicek,J., Fuks,Z., Haimovitz-Friedman,A.,
            Kolesnick,R.N. and Futerman,A.H.
  TITLE     Kinetic characterization of mammalian ceramide synthases:
            determination of K(m) values towards sphinganine
  JOURNAL   FEBS Lett 581 (27), 5289-5294 (2007)
   PUBMED   17977534
REFERENCE   6  (bases 1 to 6821)
  AUTHORS   Mesika,A., Ben-Dor,S., Laviad,E.L. and Futerman,A.H.
  TITLE     A new functional motif in Hox domain-containing ceramide synthases:
            identification of a novel region flanking the Hox and TLC domains
            essential for activity
  JOURNAL   J Biol Chem 282 (37), 27366-27373 (2007)
   PUBMED   17609214
REFERENCE   7  (bases 1 to 6821)
  AUTHORS   Mizutani,Y., Kihara,A. and Igarashi,Y.
  TITLE     Mammalian Lass6 and its related family members regulate synthesis
            of specific ceramides
  JOURNAL   Biochem J 390 (Pt 1), 263-271 (2005)
   PUBMED   15823095
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Jan 6, 2022 this sequence version replaced XM_006234308.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360172.1133629.1,
                                           SRR26643298.16163.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-337               JAXUCZ010000003.1  74038861-74039197
            338-443             JAXUCZ010000003.1  74101810-74101915
            444-574             JAXUCZ010000003.1  74109799-74109929
            575-632             JAXUCZ010000003.1  74169234-74169291
            633-683             JAXUCZ010000003.1  74213339-74213389
            684-776             JAXUCZ010000003.1  74217044-74217136
            777-905             JAXUCZ010000003.1  74235662-74235790
            906-1012            JAXUCZ010000003.1  74238358-74238464
            1013-1169           JAXUCZ010000003.1  74278465-74278621
            1170-1193           JAXUCZ010000003.1  74279209-74279232
            1194-6821           JAXUCZ010000003.1  74281917-74287544
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q21"
     gene            1..6821
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /note="ceramide synthase 6"
                     /db_xref="GeneID:366065"
                     /db_xref="RGD:1561599"
     exon            1..337
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..1346
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /EC_number="2.3.1.291"
                     /note="isoform 1 is encoded by transcript variant 1; LAG1
                     longevity assurance homolog 6; LAG1 homolog, ceramide
                     synthase 6"
                     /codon_start=1
                     /product="ceramide synthase 6 isoform 1"
                     /protein_id="NP_001386291.1"
                     /db_xref="GeneID:366065"
                     /db_xref="RGD:1561599"
                     /translation="MAGILAWFWNERFWLPHNVTWADLKNTEEATFPQAEDLYLAFPL
                     AFCIFMVRLIFERFIAKPCAVALNIQANGPQIAQPNAILEKVFTAITKHPDEKRLEGL
                     SKQLDWDVRSIQRWFRQRRNQEKPSTLTRFCESMWRFSFYLYVFSYGVRFLKQTPWLW
                     NTRHCWYNYPYQPLTADLHYYYILELSFYWSLMVSQFTDIKRKDFGIMFLHHLATIFL
                     ITFSYVNNMARVGTLVLCLHDSADALLEAAKMANYAKFQKMCDLLFVMFAVVFITTRL
                     GIFPLWVLNTTLFESWEIVGPYPSWWVFNLLLLLLQGLNCFWSYLIVKIACKTVSKGK
                     AGKWNPVHVSKDDRSDIESSSDDEDSEPPGKKLHSSTTTNGTSGTNGYLLTGPCSMDD
                     "
     exon            338..443
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            444..574
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            575..632
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            633..683
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            684..776
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            777..905
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1012
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1169
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1193
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..6821
                     /gene="Cers6"
                     /gene_synonym="Lass6; RGD1561599"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagcggatc ggcgagcctg agagtgcagc ggccgcggag gaggcggcgg caggcggagc
       61 ggcagcggca caggctcccg gccaacccag cgcgcatccc cagtgccctg cgctgcagag
      121 agctcggagg aacgcgggag cagcgacaca ggagtggaca aagcaagatg gcagggatct
      181 tagcctggtt ctggaatgag cggttttggc ttccgcacaa tgtcacctgg gcagacctga
      241 agaacacgga ggaagccacc ttcccgcagg cggaggacct ctacctcgcc ttccccttgg
      301 ccttctgcat cttcatggtg cggctcatct tcgagagatt catagccaaa ccatgtgccg
      361 tagccctcaa catccaagcc aatggaccac aaattgccca gccaaatgcc attctggaaa
      421 aggttttcac cgccataaca aagcatcctg atgagaagag attggaaggg ctctccaagc
      481 agctggactg ggatgttcgg agcattcaac gctggtttcg acaaaggcgt aaccaggaga
      541 aacccagcac tctgaccagg ttctgcgaga gcatgtggag attttccttt tacctctatg
      601 tatttagcta cggagtccgg ttcttgaaac agaccccttg gttgtggaat acaaggcact
      661 gctggtataa ctacccttac cagccactca ctgctgacct ccactactat tacatcctgg
      721 agctgtcgtt ttattggtct ttaatggttt cccagttcac agatatcaaa aggaaggact
      781 tcggcattat gttcctgcac caccttgcaa ccattttctt aataactttt tcatatgtca
      841 acaacatggc ccgagtagga accctggtcc tctgtcttca cgactcagcc gatgctctgc
      901 tagaggctgc caaaatggca aattatgcca aatttcagaa aatgtgtgat ctcctgtttg
      961 tcatgtttgc tgtggttttt atcaccacac ggctgggcat atttcctctc tgggtgttga
     1021 ataccacatt atttgaaagt tgggagatcg ttggaccgta cccatcctgg tgggttttta
     1081 acctgctgct gttgttacta caagggttga actgcttctg gtcttacctg attgtaaaaa
     1141 tagcttgcaa aactgtttca aaaggcaagg ccgggaagtg gaaccctgta catgtgtcca
     1201 aggacgaccg gagcgacatt gaatctagct cagatgatga ggactcggag cctccaggaa
     1261 agaaactaca ctcttcgaca accaccaacg gaaccagcgg taccaacggg tacctcctca
     1321 ctggcccttg ctccatggat gattaattac ccaaaactat aagttctgaa caaagggaac
     1381 tgtttgttcc tggaagtatt taataagttg caaatgcagt tcctttcatg atatctcatc
     1441 accagagaca aaagttagga ttctcagagc gttctgaata gtgtactgcc atgcgtcctg
     1501 tctgtgaatg aagaactccc attctttctg tgtaggcatg ctgtatgtaa ctgacacaag
     1561 ggaacggtat ttgcatttgt cttaggatat tatttatttt tttatttgtt tgtaaatctg
     1621 tggacagagg aggatcccct cactcctttt attctctggg ctcatgattg gcagtaaaaa
     1681 tgtcccatct gtgtcccttt accacgctgt agtccagtgt gccatgagca ccaactttac
     1741 tttaccccgt agagcaagta aaatcccatg caagatcctc acacagttcc cagtaggttg
     1801 gctttctttc tgttctgcac ccattttata attgcccatg tagttggtga tcgttgtccc
     1861 acgtgagtct aaacaggatg aactgggcca ctctttgtga tcagttagca gttccaataa
     1921 aagagggtgg ctattggggg acttttgaat ttttacagga aactgccttg gaatagagtt
     1981 tgtctcttgt acacaagcat gaaatttcct ttctgtggta ggtggttggg agttgaccat
     2041 gttggtaaca aagttatttt tgccctccag tgaagtcagt gttctgatta tgaaacaatg
     2101 ccacttgctt aagttcccag ataccaaggg acccacaaat tatcttctgg ggctctcata
     2161 aagatagcca ctgacttcaa cttgccataa tttctactgt attagcccag tcttctgtcc
     2221 taacatataa aaaagaggaa gtcaagaata ttctgaggca gaaaaataaa aaggtttgtg
     2281 ggagttggca aagacttcct tccctgcaaa gcactttatt gcaaccagct ttcaagagaa
     2341 aagccctatc tagtattaga ctttgtattt tgctgagcaa agtaaatcaa atgggagaac
     2401 gactattaac cctttgcctt cctcctcaaa tgcatcccac aatgactttc ttgttgctag
     2461 aagcatccac cccacaagac aagtccattc tatttactgc tctgatgact ggtgggggct
     2521 catgaatttc ttttcaagtg gagccatttc cagagaaaca gtaaaaattc tactggagaa
     2581 tggcagggac agctgtgggg gaaattacca aagagtaagt catgagcctc ccacccccac
     2641 ccaccctctg cagccagtgt cacttctgtg cagctacaaa aaccacacag atgagccagg
     2701 gatttttatc ccatctcatc ttcaggaaaa ggggaactga atggaattaa agcactcatg
     2761 agtcaaacat cccctaaaac aacactccaa accagtcagg gcactgagta tccaatagta
     2821 aaagattgcg gtgcctgaca gtcctgctca gagccaccta tactccgttt tgttcttaaa
     2881 tcattatacc tgtactctga agcctatgag ttaatagtta agagaagact cccaaagaac
     2941 tcttgctgca acattaaatc tttctccacc taattacaaa acagtatttc ctggtatggt
     3001 ccagacaata attttaaaag cttggaaaca tgtctacttg ctgctgtaga agacagtgca
     3061 actgactctg tgctttgagc cttgctgatt tattaaccac aacagttatg cgtctaagga
     3121 gcgaaacttg aaagtatttg agtttgtgag gccaatgatg agaccccagt ggtattgtta
     3181 gtagtcagag catttgctca aatgcagatg gatgaaaaac aatagtagca gtgtcaagac
     3241 agtgaaactt tgtcatcagc taagctaagg agcctgccca cctccagtta atatcttggc
     3301 acatcctccc aggggtgaga tggttttgac aaccgctgtg tacttgtatt tgaaccgtgt
     3361 gacactcttt agcacagaag aattttaaga tcaatctgta aattcagaaa aggtatataa
     3421 aataaagtaa gttgattctg tctgtatcaa aggcagatct aaaccatctt ttccacagaa
     3481 gtcactgtgg aagacaaggg acacgatttc tgaccgacgc cattagcttg gcttggtctt
     3541 caacgtgtct attgattttt gtttcatttt accttttaga ggaaataatt ctgtttggca
     3601 attgaaatgg agtttgtagg cttgttctag aatagaatat taatatctct gtctaatgtt
     3661 ctcccaaaat gaatggataa gactgagaac agacactgtc tgaggaatct tgtccacata
     3721 taccaggcca ttgctctcag aagtctgact tgtaaatcca gctttaggtt agttctgagt
     3781 ggtgttcttg ttaattagct tagcttgtgg tgatgtctcc tccttcctgg ttgcataaga
     3841 tttcccaggg taccaaagtg tgtgtaggaa ggactctaga aaaagtaact ttcatagctt
     3901 gcctagattt agtggggggg aacagtagga aatcaggtag aatttggcct tgagtcaata
     3961 aatgatatag ccaagtatga tttacgtgtg catatgtatg tttcttcaaa aactggcaaa
     4021 actaaattat tactgattct tcttcttctt cttcttcttc ttcttcttct tcttcttctt
     4081 cttcttcttc ttcatcatca tcatcatcat catcatcatc catattcctg tccttaaaaa
     4141 aaatacactc aaccatgttc tatacaaatt gcccttctta gtttggcttc gtaccagctg
     4201 agagtagctt tcagccttcc actcacagag ctccctgaga tagagcccag gtcctggagc
     4261 atctgctgcc acacataaga cacacccagc tctctctcac agatcctatc ctgtgggtgt
     4321 tgagagcaga ggagcagcta caagaatcag tattgtgggt cattccagtg tttattgtaa
     4381 aatgcaagtg agtgccattt aaccccatga ttctaatgtc tgctgaacga ccagacaggg
     4441 catatcccag attatcatta gcgtgtgcca ttggcatcta acacagatga acaaagcagc
     4501 aatggcaggg gccgaggaag accagatcac atctttagtc catacggtcg gtgagtctca
     4561 aactgttgtc tgagtcaaat acttggcaac agtactgtat gctaccatgg tcccaaacag
     4621 gatgacatag cctaggcaaa gaggcaacat tgtctgcatg tgtgcaagta cacttgttga
     4681 tgttcacaga gtgacaaaga tgtgtccctg ttgtatgaga catgcctata cttgacaaga
     4741 gtttgagttt gagcaagctt aatgccttcc ctcagtttag ataaaagggt ctgaatcttc
     4801 caccttgctt ttaaatctag ttgttgtttg ggctaaggaa aaacgagact tcattattaa
     4861 aaaggacatt ggagttcacc tgtgtcttgg ctggcaaact cttctgagag aatatgggaa
     4921 ccctcggttg tctccaaccc agtcatcagt ttgtattact aatcatttgg gggtgaatta
     4981 atgccaaatc tgcctggata tttgaatgtt ttccttaatt tgaagaacag tatacagaat
     5041 taccccaaag gtatggatga gggccattta tcagtatggg agatgatgtc attataccca
     5101 aagaggcctc aatgcctttg gaaataagaa gttaagtgtt tgtggtatta aggctcctac
     5161 ttactcactt ttcttgtctt tggcttactt ctgcctagtc aataagtcat catagccctg
     5221 caatgtttct aacacccaaa tgagagaaaa catcaaacaa gaccagagtc agcctgaggg
     5281 gaaagtgatc acatggttct tttgtagcag ggaagaaatg cagagggctc taactactgc
     5341 agttgccttc aagtatgttg ttcctgtcag gaacgatagt aaaagttctt aaagtgaaac
     5401 aagacatttt ccttaaatgt gttcttccca ggatcagctt ttcttccaga gatgaaattg
     5461 tggtgttctc caaatgctgt agactaatta gcaatactct gccaatcaca ttttagttcc
     5521 atgaacaaat gggctaaagg tgggccctgt accttgtcta tgtgtacata gcacccaccc
     5581 ctagggggcg ccaagcagat gtgagggagc cctaactaaa gctcttgaga ttcctgtcac
     5641 caatactcaa aggcagccac ttctcatgaa gaaaatcaga ttgaagaaca taagtcacac
     5701 cataagtaat gagatcttgt agacacccaa atgagaagat tcacctcccg cgggctttcg
     5761 tgacttggtt taacccaaag cctttgacag gaatgcattg gcagatgggt gcactgagag
     5821 gccacccaca ctgagaaatt gaaaagtctg ggcagggtta catatggcat ccgcatagaa
     5881 ccaggcactc tggggattgc tgggaagtga tgagagggct ctgttagcct ggagctggtc
     5941 tcctttatct cattccctca cagaccgtta ggcttctggg actctggtaa aagaaagcct
     6001 catgctagcg ttctttctgc tttcagttta ctaatgactg ttagatcggc tcttcccacc
     6061 ccttctctct gtctgatccc attttcagag ggcaggggag gctgtgtcca tgtacacctg
     6121 tttccttaac acaggtccac gaatgcttgg cttcctgtat tgtatgctgt ctgggaacca
     6181 tggtgcagca tcagtcatca tagttcctgt gctcagtgaa agaatttggt ttcactcatt
     6241 tttatcctct ttccccacca taaatgtcag aaactctgtt aaactgtcct taatgtgaag
     6301 cttttgtgtt ttttgggttt tctgttttgt tttgttttct ttggtttttt tttagcgtta
     6361 gctacaaagc tctggaatgg ttacattatg attctggaat gtttgctcac agcttaatta
     6421 acataaatgt ggagcacact ccagttggat tcccgtgtga cacagttctc attactcctg
     6481 tgcccaagtc ctcgtcatta ccttcatggc cttgagctgt cttagaaaag gatggtggtt
     6541 catttgggga tttgtttctc ttttccagct ttcagtgggg actaggataa gtgacaaaac
     6601 atcatttgtt ttctaaagac agttcagtgc gtgagcatct gtcagaagtg gaatggtgta
     6661 ccattagcca attagaatta ctccgtgtac tgttgctgtg ttttgctgat ttttatatgg
     6721 ggatataatt acttgttctt gatctttgga agtaattgtt gttatgagga tcattttact
     6781 ttggaaacac tttgataata aagttaagca ttaagggtac a
//
