LOCUS       NM_001108993            2655 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus ceramide synthase 5 (Cers5), mRNA.
ACCESSION   NM_001108993 XM_001063108 XM_345870
VERSION     NM_001108993.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Tidhar,R., Zelnik,I.D., Volpert,G., Ben-Dor,S., Kelly,S.,
            Merrill,A.H. Jr. and Futerman,A.H.
  TITLE     Eleven residues determine the acyl chain specificity of ceramide
            synthases
  JOURNAL   J Biol Chem 293 (25), 9912-9921 (2018)
   PUBMED   29632068
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Novgorodov,S.A., Chudakova,D.A., Wheeler,B.W., Bielawski,J.,
            Kindy,M.S., Obeid,L.M. and Gudz,T.I.
  TITLE     Developmentally regulated ceramide synthase 6 increases
            mitochondrial Ca2+ loading capacity and promotes apoptosis
  JOURNAL   J Biol Chem 286 (6), 4644-4658 (2011)
   PUBMED   21148554
REFERENCE   3  (bases 1 to 2655)
  AUTHORS   Lahiri,S., Lee,H., Mesicek,J., Fuks,Z., Haimovitz-Friedman,A.,
            Kolesnick,R.N. and Futerman,A.H.
  TITLE     Kinetic characterization of mammalian ceramide synthases:
            determination of K(m) values towards sphinganine
  JOURNAL   FEBS Lett 581 (27), 5289-5294 (2007)
   PUBMED   17977534
REFERENCE   4  (bases 1 to 2655)
  AUTHORS   Mesika,A., Ben-Dor,S., Laviad,E.L. and Futerman,A.H.
  TITLE     A new functional motif in Hox domain-containing ceramide synthases:
            identification of a novel region flanking the Hox and TLC domains
            essential for activity
  JOURNAL   J Biol Chem 282 (37), 27366-27373 (2007)
   PUBMED   17609214
REFERENCE   5  (bases 1 to 2655)
  AUTHORS   Spassieva,S., Seo,J.G., Jiang,J.C., Bielawski,J.,
            Alvarez-Vasquez,F., Jazwinski,S.M., Hannun,Y.A. and Obeid,L.M.
  TITLE     Necessary role for the Lag1p motif in (dihydro)ceramide synthase
            activity
  JOURNAL   J Biol Chem 281 (45), 33931-33938 (2006)
   PUBMED   16951403
REFERENCE   6  (bases 1 to 2655)
  AUTHORS   Lahiri,S. and Futerman,A.H.
  TITLE     LASS5 is a bona fide dihydroceramide synthase that selectively
            utilizes palmitoyl-CoA as acyl donor
  JOURNAL   J Biol Chem 280 (40), 33735-33738 (2005)
   PUBMED   16100120
REFERENCE   7  (bases 1 to 2655)
  AUTHORS   Mizutani,Y., Kihara,A. and Igarashi,Y.
  TITLE     Mammalian Lass6 and its related family members regulate synthesis
            of specific ceramides
  JOURNAL   Biochem J 390 (Pt 1), 263-271 (2005)
   PUBMED   15823095
REFERENCE   8  (bases 1 to 2655)
  AUTHORS   Riebeling,C., Allegood,J.C., Wang,E., Merrill,A.H. Jr. and
            Futerman,A.H.
  TITLE     Two mammalian longevity assurance gene (LAG1) family members, trh1
            and trh4, regulate dihydroceramide synthesis using different fatty
            acyl-CoA donors
  JOURNAL   J Biol Chem 278 (44), 43452-43459 (2003)
   PUBMED   12912983
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474035.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_345870.3, XM_001063108.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760396,
                              SAMEA5760434 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q36"
     gene            1..2655
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /note="ceramide synthase 5"
                     /db_xref="GeneID:366984"
                     /db_xref="RGD:1311597"
     exon            1..333
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..1486
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /EC_number="2.3.1.24"
                     /note="longevity assurance homolog 5; LAG1 longevity
                     assurance homolog 5; LAG1 homolog, ceramide synthase 5"
                     /codon_start=1
                     /product="ceramide synthase 5"
                     /protein_id="NP_001102463.1"
                     /db_xref="GeneID:366984"
                     /db_xref="RGD:1311597"
                     /translation="MATAAAEALGLLWGWLWSGRFWLPQNVSWADLEGPGDGYGYPRA
                     RHVLSVFPLAAGLFSVRMLFERFIAKPCALRIGIEDSAVYQVESNVTLEKVFTSISKY
                     PDEKRLKGLSKQLDWDVRKIQCWFRHRRNQDKPPTLTKFCESMWRFTFYFCIFCYGVR
                     FLWSMPWLWDTRQCWYNYPYQPLSRELYYYYLTQLAFYWSLVFSQFIDVKRKDFLMMF
                     MHHLIAVTLISFSYINNMVRVGAIILCLHDSADSLLEAAKLANYARQERLCNTLFVIF
                     GAAFMVTRLGIFPLWILNTTLFESWEIIGPFPSWWLFNGLLLILQMLHVIWSYLIART
                     AFKALVRGKVTYPGGISPGSVPSALFSPPSSFLCLVAAGQSHPLHVSKDDRSDVESSS
                     EEDETTHTNNLSGSSSSNGANCMNGRMGGSHLAEEQGTCKAAGNLHFGASPRIHSCD"
     exon            334..439
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            440..570
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            571..628
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            629..679
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            680..772
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            773..901
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1008
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1165
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1273
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..2655
                     /gene="Cers5"
                     /gene_synonym="Lass5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggcgtctgg gaggacccga ggacggaaga gggtggccgt aggccgggcc ccgccccggg
       61 gccgccttcc agcgggttct ttttgccttg gctgcctcgg aggctgtggc ggcagtggcc
      121 gccgggcgcg cgtaagatgg cgactgcggc ggcggaagcc ctgggcctcc tgtggggctg
      181 gctgtggagc gggcgcttct ggctgcctca gaacgtgagc tgggcggacc tggagggccc
      241 gggcgacggc tacggctacc cgcgcgcccg gcacgtgctg tcggtgttcc cgctggcagc
      301 aggtctcttc tccgtgagga tgctgttcga gcgatttatt gctaaaccct gtgcactccg
      361 tattggcatt gaagacagtg ctgtctacca ggtggaatcc aatgtcaccc ttgagaaggt
      421 gttcacatct atctctaagt atcctgatga gaaaaggctg aagggcctat caaagcaact
      481 ggactgggat gttcgaaaaa tccaatgctg gtttcgccat cggagaaatc aggacaagcc
      541 tccaacgctc actaagttct gtgagagcat gtggagattc actttctatt tttgtatctt
      601 ctgctacgga gtcagatttc tctggtcgat gccttggctc tgggataccc gacagtgctg
      661 gtacaactat ccatatcagc cactctcaag agagctctat tactactatc tcacacagct
      721 ggccttctac tggtccctcg tgttctctca gtttatagat gttaaaagaa aggacttcct
      781 gatgatgttt atgcatcatt tgatcgccgt cacgctcatc agtttctcct acatcaacaa
      841 catggtgcga gtgggagcca tcatcttgtg tctgcatgac tccgccgact ccttgctaga
      901 agctgccaag ctggccaatt acgccagaca ggagcggctc tgtaacaccc tctttgtgat
      961 ctttggtgca gcttttatgg ttactcgtct agggatcttc cctttgtgga ttctgaacac
     1021 aaccctcttt gagagctggg agattattgg gccctttcct tcctggtggc tcttcaatgg
     1081 cctcctcctg atcctacaga tgttgcatgt catctggtcc tacctaatcg cacgaactgc
     1141 ttttaaagcc ttggtccggg gcaaggtgac ctacccagga gggattagcc ccggctctgt
     1201 accctctgct cttttctcac ctccttcctc ttttctatgc ctggtggcag ctggacagtc
     1261 ccatcctctg catgtgtcta aggatgaccg cagtgatgta gaaagcagct cagaggaaga
     1321 tgagaccaca cacacgaaca acctctctgg cagcagttcc agcaacggtg ccaactgcat
     1381 gaacggccgc atgggaggca gccacttggc tgaggagcag gggacttgta aagctgctgg
     1441 caacctacac tttggggcct ccccacgtat acactcctgt gactagaggg accgccaggc
     1501 actgaggaga atctgagaaa caaatgtttt tgtgttttaa agaaactctg ccattttgta
     1561 tttaatgccc tggtccttca gtgacatcac tggctctttg tctgtgtgtc tgtgcgtctg
     1621 tacatgtgca tcatgcatgt ggatgagcgt catccactag gttgggcaca aggctggatt
     1681 actagtcagt gtctggtcac ttctgaatca cctccggtcc agcgttcgct gtatcttgag
     1741 ttaagctgtc tcccaaccag ctgtgtcctc tttccgtggc tcttgagact cttcagttcc
     1801 tggtccatgg aagtcagtgg tgcggccact tggctccagc acttggagac tgttcttttg
     1861 gaggaggaag taattttcac ctgatggaac actctggata gacaccagga tcactgtagc
     1921 cacatgcccc cttcctgcca cccgacagtg tcagggacag ccactgacgc cctgctgctt
     1981 attcaatggt acgcagggag gggaggggag gggagggtga gctgagattc ggagaacagc
     2041 aaccactggt tgtgctgtta atgattttat actattgttt attcttctgt gattaaagtg
     2101 tttcaaccct ctactgtctg tctctttagt aactatccct gctgcagctt tggctgcctg
     2161 agcctcctca gtgctgaggt catagacatg ctccatgatt aggaagtttt ccatgggctg
     2221 gagagttctt gcagaggacc cagctctggt ctctagccct gatacagggt tttaatgtag
     2281 acatatttaa ttggtgtgct agcaggtttt atatcaactt gacacaagtt atagtcactt
     2341 tgaaaagagg gaccctcaga tgagaaaact cccctagatt ggccagtggg caagcctata
     2401 tagagcattt tcttaactga tgattgactg ggggcaggca gcccattgtg ggtatggggc
     2461 catccctgaa ctgttggtcc tggggtttat gaggaatcag agcaagccat ggggagcaag
     2521 ccaagggcag caccctccat gacctctgca tgagctcctg cctccaggtc cctgccctat
     2581 gtgagttcct gcccagactt gcttctgtga tggacaatgc agtggaagtg taagctgaat
     2641 aaacccattc ttccc
//
