LOCUS       NM_001107117            3395 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus ceramide synthase 4 (Cers4), mRNA.
ACCESSION   NM_001107117 XM_001065770 XM_006248805 XM_221796
VERSION     NM_001107117.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Tidhar,R., Zelnik,I.D., Volpert,G., Ben-Dor,S., Kelly,S.,
            Merrill,A.H. Jr. and Futerman,A.H.
  TITLE     Eleven residues determine the acyl chain specificity of ceramide
            synthases
  JOURNAL   J Biol Chem 293 (25), 9912-9921 (2018)
   PUBMED   29632068
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Lahiri,S., Lee,H., Mesicek,J., Fuks,Z., Haimovitz-Friedman,A.,
            Kolesnick,R.N. and Futerman,A.H.
  TITLE     Kinetic characterization of mammalian ceramide synthases:
            determination of K(m) values towards sphinganine
  JOURNAL   FEBS Lett 581 (27), 5289-5294 (2007)
   PUBMED   17977534
REFERENCE   3  (bases 1 to 3395)
  AUTHORS   Mizutani,Y., Kihara,A. and Igarashi,Y.
  TITLE     Mammalian Lass6 and its related family members regulate synthesis
            of specific ceramides
  JOURNAL   Biochem J 390 (Pt 1), 263-271 (2005)
   PUBMED   15823095
REFERENCE   4  (bases 1 to 3395)
  AUTHORS   Riebeling,C., Allegood,J.C., Wang,E., Merrill,A.H. Jr. and
            Futerman,A.H.
  TITLE     Two mammalian longevity assurance gene (LAG1) family members, trh1
            and trh4, regulate dihydroceramide synthesis using different fatty
            acyl-CoA donors
  JOURNAL   J Biol Chem 278 (44), 43452-43459 (2003)
   PUBMED   12912983
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On or before Dec 8, 2020 this sequence version replaced
            XM_006248805.3, NM_001107117.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.2373056.1,
                                           SRR26360186.1567306.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760433, SAMN12840106
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                JAXUCZ010000012.1  7649610-7649652
            44-229              JAXUCZ010000012.1  7649992-7650177
            230-403             JAXUCZ010000012.1  7674266-7674439
            404-521             JAXUCZ010000012.1  7675601-7675718
            522-640             JAXUCZ010000012.1  7677184-7677302
            641-698             JAXUCZ010000012.1  7677580-7677637
            699-749             JAXUCZ010000012.1  7678427-7678477
            750-842             JAXUCZ010000012.1  7679172-7679264
            843-971             JAXUCZ010000012.1  7679511-7679639
            972-1078            JAXUCZ010000012.1  7680113-7680219
            1079-1235           JAXUCZ010000012.1  7682244-7682400
            1236-3395           JAXUCZ010000012.1  7682494-7684653
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12p12"
     gene            1..3395
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /note="ceramide synthase 4"
                     /db_xref="GeneID:304208"
                     /db_xref="RGD:1309303"
     exon            1..43
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            44..229
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            230..403
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     CDS             231..1412
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /EC_number="2.3.1.24"
                     /note="longevity assurance homolog 4; LAG1 homolog,
                     ceramide synthase 4; LAG1 longevity assurance homolog 4"
                     /codon_start=1
                     /product="ceramide synthase 4"
                     /protein_id="NP_001100587.1"
                     /db_xref="GeneID:304208"
                     /db_xref="RGD:1309303"
                     /translation="MLFSLNEWLWQETYWLPPNVTWAQLEDRDGLVFAHPHHVLAALP
                     IALVLVAMRIIFERFVALPLSRWMGVQDPIRRKIKPNPVLEKYFLRMKQRPVETQMVL
                     LASQCGLTLRQIQRWFRRRRNQDRPCLSKKFCEASWRFVFYLCSFVGGTSILYHESWL
                     WTPALCWENYPHQTLNLALSWWYLLELGFYISLLITLPFDIKRKDFKEQVAHHFVTVG
                     LIVFSYSVNLLRIGSVVLLLHDCSDYLLEGCKMLNYAHFQRGCDTLFIIFSLVFFYTR
                     LVFFPTEVIYTAVFDSIKNSGPFFGYYFFIVLLGMLQILHVYWFCLILRMICSFLRKG
                     QMREDIRSDVEESDSSDDEAVSEGPQLKNGMARGSRAAITNGPRSRAAACLTNGHTRA
                     T"
     exon            404..521
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            522..640
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            641..698
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            699..749
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            750..842
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            843..971
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1078
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1235
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..3395
                     /gene="Cers4"
                     /gene_synonym="Lass4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggccttggc agtcaaagga agaggctgaa agcagggatt gggaccacgc ccaaggcccc
       61 atcaaactcc tcccggaggc cccctccggt cccatttcct ctctcgcggg tggaggctta
      121 gtagagagga gtttctcggc ccaacgcggt ctgtggcgtg tctacggtgt cttcgggtta
      181 gctggtcccc gcaccgcgtc ggcctgcatc ctgctgtctg cacccccagc atgctgttca
      241 gcttgaatga gtggctgtgg caggagacat actggttacc acccaatgtc acatgggcac
      301 agctggagga ccgtgatggc ctggtgttcg cccaccctca ccacgtgctt gctgccttgc
      361 caattgcact ggtcctggtg gctatgcgta tcatctttga gagattcgtg gccttgcccc
      421 tgagccggtg gatgggtgtg caggatccaa tcaggagaaa gataaagccc aacccagtgc
      481 tggagaaata cttcctcagg atgaagcaga gaccagtgga gacccagatg gtcctcctgg
      541 cttcccagtg tggcctcacg ttgcggcaga ttcaacgctg gttcaggaga cgtcggaacc
      601 aggatcggcc ttgcctgtcc aagaaattct gtgaagccag ctggaggttt gtcttctatc
      661 tgtgttcctt cgtgggtggc acctctatcc tctaccatga gtcatggttg tggacaccgg
      721 cattgtgctg ggagaattac ccacatcaga ccctgaattt ggccctgtcc tggtggtacc
      781 tcctggagct gggcttctac atttcactgc taatcactct gccctttgac atcaaacgca
      841 aggacttcaa ggagcaggtg gcgcaccact tcgtgaccgt gggactgatc gtcttctcct
      901 acagcgtcaa cctcctccgc attggctctg tggtactgtt gttgcatgac tgctccgact
      961 acctgctgga gggctgtaag atgctcaact atgcccactt tcagcgaggg tgcgatactc
     1021 ttttcatcat cttctcctta gtcttcttct acacacgcct cgtattcttc cccaccgagg
     1081 tcatctacac tgctgtgttt gactcaatca agaactcagg ccccttcttt ggctactact
     1141 tctttattgt gctcctgggg atgctacaga ttcttcatgt gtactggttc tgcctcatcc
     1201 tgcgcatgat ctgcagtttc ctgaggaagg gtcagatgag agaggacatt cgtagtgatg
     1261 tggaagaatc agactccagt gatgacgagg cagtttctga aggtccccag ctgaagaatg
     1321 ggatggctcg agggtctaga gcagccatta ccaatggccc gcggagccgg gcagcagctt
     1381 gcctgaccaa tgggcacacc cgggccacat agacaaccgc agattgacgt tgaggggttg
     1441 cctccagcat tgtggatatt tagatgctgg actggtaact ctgggagaca gggaggccct
     1501 accttaaagt aggaagaaga ctggtgatct gtttcccttc tgttcttctc actttatccc
     1561 tttgaacaac ttgacagaac ctgggggagc agtggactct gcatctggcc agagccaccc
     1621 ccaggctata gcttgggggc tctggggagc cttggctcca aggaccaata aaacttcaac
     1681 agtgacgaat tctctgccgc aaagcttgag ttcccttcac agctcttcct ctccattact
     1741 tacagtgttg acaagattcc tcactgccac atcaggattt atgtccactc ttgccggtat
     1801 agcaccacag catgaagata ctccctgctc ctcagtcagt gccttcaatt tgttctcctt
     1861 tagagtatcc ctccaggaca ttagcattag ccgatacagg ctgactttta ggaaagagat
     1921 tattaagctt agatgcaacc tctctctctg tctgcagtcc aagccagagg gtagagctga
     1981 aacctgtgga tatccttgta gtccttcgct taaagcaacc tgcagcccac cctggggtct
     2041 ttatactccc ttgggatggc ggagagctaa gatttggtat gaggttactg tcttcaggag
     2101 tgctatagga aaagatcctc ctccaagatt ggagggtggg tgaccagtgt tacttaactc
     2161 agaaatcaaa gctagaggat ccatgtatgg ttagactgtg ccatctttgg gagcaagagg
     2221 aaccttgtgt cgtgggagaa agagaagaga gtggggagaa gaggtcgtct caggggactt
     2281 ccaaaaataa aggctcttgg tacaaagagc agagcctggc atggtgaccc atggctatct
     2341 ttaacatcag agaggaagaa tagactggaa gaacatatga acctgggata caaagcaggg
     2401 cctggtctca aaacaaacaa caggtgggcg tgtcaaggaa caggtactcc agaagagaaa
     2461 acaatcggaa tccgagatag aaacagctaa gaaaaggatg ggaaaagaat gaggggtgac
     2521 tctgggtgca gagcaggttg gtgaggtgag atacggagtt ttgtcttttg gagaaatatg
     2581 gggaaaagga agagaaatgt ggcttctgtt gagagaacaa cagtaggaga aacagaagta
     2641 acactggtag agtatgcata gggacttgtg aaagagacag gaagacaaag caactgatag
     2701 ggaaggaaag ggggttggga gggatgaaga aacaacaaca acaccaaaaa gaacagaaag
     2761 aggaaaatgg tgttgatggt gcatggatga gaaaactgga aaagatcggg aaagggtatt
     2821 tgagaaggaa ggcagagggc cattgaccag agtaacagct tctagagaac ttgagggatg
     2881 gaaacaagat gtggccacaa gtctgaaatc acagtactcc tgatgctgag gcagaaagat
     2941 cctaggtttg aggccagcct gggaaggagt accaacctaa aaaagcaaca actgcaacag
     3001 gaaatgcatg gaggtataca tggacacatg gatttctgtt tttctttttc ttttcttttt
     3061 tctttttagc tgtgaagaat aaatcatgat agttgcagaa aaatggatgc aactggaagc
     3121 catgtttggt agagtaaaac agtcagacaa atactgaatg ttttttatgc aaaaattaga
     3181 ttcaaatgat aaatgtttgt gtatatgtgt tttttgcatt tgtatgttta taggtcatga
     3241 aaatagaggg gtcatgggtc aggcatggtg ttacacgcct ttaatcctag cacttgggag
     3301 gcagaggcag gcagatctct tgagtttgag gccagcctgg tcaacaaagc aaatccagga
     3361 cagggctgtt acatagagaa accccatctt gaaaa
//
