LOCUS       NM_001033700            2034 bp    mRNA    linear   ROD 28-MAR-2023
DEFINITION  Rattus norvegicus ceramide synthase 2 (Cers2), mRNA.
ACCESSION   NM_001033700 XM_227443
VERSION     NM_001033700.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Thamizhoviya G and Vanisree AJ.
  TITLE     Enriched Environment Enhances the Myelin Regulatory Factor by mTOR
            Signaling and Protects the Myelin Membrane Against Oxidative Damage
            in Rats Exposed to Chronic Immobilization Stress
  JOURNAL   Neurochem Res 46 (12), 3314-3324 (2021)
   PUBMED   34449011
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Tidhar R, Zelnik ID, Volpert G, Ben-Dor S, Kelly S, Merrill AH Jr
            and Futerman AH.
  TITLE     Eleven residues determine the acyl chain specificity of ceramide
            synthases
  JOURNAL   J Biol Chem 293 (25), 9912-9921 (2018)
   PUBMED   29632068
REFERENCE   3  (bases 1 to 2034)
  AUTHORS   Novgorodov SA, Riley CL, Keffler JA, Yu J, Kindy MS, Macklin WB,
            Lombard DB and Gudz TI.
  TITLE     SIRT3 Deacetylates Ceramide Synthases: IMPLICATIONS FOR
            MITOCHONDRIAL DYSFUNCTION AND BRAIN INJURY
  JOURNAL   J Biol Chem 291 (4), 1957-1973 (2016)
   PUBMED   26620563
REFERENCE   4  (bases 1 to 2034)
  AUTHORS   Yamashita-Sugahara Y, Tokuzawa Y, Nakachi Y, Kanesaki-Yatsuka Y,
            Matsumoto M, Mizuno Y and Okazaki Y.
  TITLE     Fam57b (family with sequence similarity 57, member B), a novel
            peroxisome proliferator-activated receptor gamma target gene that
            regulates adipogenesis through ceramide synthesis
  JOURNAL   J Biol Chem 288 (7), 4522-4537 (2013)
   PUBMED   23275342
  REMARK    Erratum:[J Biol Chem. 2020 Apr 10;295(15):5176. PMID: 32277067]
REFERENCE   5  (bases 1 to 2034)
  AUTHORS   Yu B, Zhou S, Wang Y, Qian T, Ding G, Ding F and Gu X.
  TITLE     miR-221 and miR-222 promote Schwann cell proliferation and
            migration by targeting LASS2 after sciatic nerve injury
  JOURNAL   J Cell Sci 125 (Pt 11), 2675-2683 (2012)
   PUBMED   22393241
  REMARK    GeneRIF: Sciatic nerve injury promotes Schwann cell proliferation
            and migration through the regulation of miR-221/222 by targeting
            LASS2
REFERENCE   6  (bases 1 to 2034)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 2034)
  AUTHORS   Laviad EL, Albee L, Pankova-Kholmyansky I, Epstein S, Park H,
            Merrill AH Jr and Futerman AH.
  TITLE     Characterization of ceramide synthase 2: tissue distribution,
            substrate specificity, and inhibition by sphingosine 1-phosphate
  JOURNAL   J Biol Chem 283 (9), 5677-5684 (2008)
   PUBMED   18165233
REFERENCE   8  (bases 1 to 2034)
  AUTHORS   Lahiri S, Lee H, Mesicek J, Fuks Z, Haimovitz-Friedman A, Kolesnick
            RN and Futerman AH.
  TITLE     Kinetic characterization of mammalian ceramide synthases:
            determination of K(m) values towards sphinganine
  JOURNAL   FEBS Lett 581 (27), 5289-5294 (2007)
   PUBMED   17977534
REFERENCE   9  (bases 1 to 2034)
  AUTHORS   Mizutani Y, Kihara A and Igarashi Y.
  TITLE     Mammalian Lass6 and its related family members regulate synthesis
            of specific ceramides
  JOURNAL   Biochem J 390 (Pt 1), 263-271 (2005)
   PUBMED   15823095
REFERENCE   10 (bases 1 to 2034)
  AUTHORS   Shultz MA, Zhang L, Gu YZ, Baker GL, Fannuchi MV, Padua AM, Gurske
            WA, Morin D, Penn SG, Jovanovich SB, Plopper CG and Buckpitt AR.
  TITLE     Gene expression analysis in response to lung toxicants: I.
            Sequencing and microarray development
  JOURNAL   Am J Respir Cell Mol Biol 30 (3), 296-310 (2004)
   PUBMED   12947022
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK365108.1, BC101876.1, CF110567.1 and BI284504.1.
            
            On Aug 12, 2008 this sequence version replaced NM_001033700.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-697               CK365108.1         1-697
            698-1097            BC101876.1         643-1042
            1098-1785           CF110567.1         78-765
            1786-2034           BI284504.1         1-249               c
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..2034
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /note="ceramide synthase 2"
                     /db_xref="GeneID:310667"
                     /db_xref="RGD:1310059"
     exon            1..94
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            95..268
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1238
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /EC_number="2.3.1.24"
                     /note="LAG1 homolog, ceramide synthase 2"
                     /codon_start=1
                     /product="ceramide synthase 2"
                     /protein_id="NP_001028872.2"
                     /db_xref="GeneID:310667"
                     /db_xref="RGD:1310059"
                     /translation="MLQTLYDYFWWERLWLPVNLTWADLEDKDGRVYAKASDLYITLP
                     LALLFLIIRYFFELYVATPLAALLNVKEKTRLRAPPNATLEHFYLTVGKQPKQVEVDL
                     LSRQSGLSGRQVERWFRRRRNQDRPSLLKKFREASWRFTYYLIAFVAGMAVTVDKPWF
                     YDLRKVWEGYPIQSIIPSQYWYYMIELAFYWSLLFSIASDVKRKDFKEQIIHHVATII
                     LLCFSWFANYVRAGTLIMALHDSSDYLLESAKMFNYAGWKNTCNNLFIVFAVVFIITR
                     LVIMPFWILHCTMIYPLEFYPAFFGYYFFNSMMVVLQMLHVFWAYFIVRMAHKFITGK
                     LVEDERSDREETESSEGEEAAAGAGAKSRLLANGHPILNNNHPKND"
     exon            269..386
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            387..505
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            506..563
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            564..614
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            615..707
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            708..836
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            837..943
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1097
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..2022
                     /gene="Cers2"
                     /gene_synonym="Lass2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2014..2019
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers2"
                     /gene_synonym="Lass2"
     polyA_site      2022
                     /gene="Cers2"
                     /gene_synonym="Lass2"
ORIGIN      
        1 aggacccggc ccagtcagtg cgcactggcc gggcgctaga agtgggacac ggagcagcgg
       61 cgcccggacc ggtcagcttt gcactggccg ccgagatgct ccagaccttg tatgactact
      121 tctggtggga acgactatgg ctgcctgtga acttaacctg ggctgatcta gaagacaaag
      181 atggacgtgt ctatgccaaa gcctcagacc tctacatcac actacccctg gccctgctct
      241 ttctcatcat tcgatacttc tttgagcttt atgtggctac acccctggct gccctcctga
      301 atgttaagga gaaaacccga ctgcgggcac ctcccaatgc caccttagag catttctacc
      361 tgaccgtcgg caagcagccc aagcaggtgg aggtagacct tttgtctcgg cagagtgggc
      421 tctctggacg ccaggtagaa cgttggttcc gccgccgccg caaccaggac aggcccagtc
      481 ttctcaagaa gttccgagaa gccagctgga gattcacata ttacctcatt gcctttgttg
      541 ctggcatggc tgtcactgtg gataaaccct ggttctatga cttgaggaaa gtttgggaag
      601 gatatcccat acagagtatc atcccttctc agtattggta ctacatgatt gagcttgctt
      661 tctactggtc cctgctcttc agcattgcct ccgatgtcaa gcgaaaggat tttaaggaac
      721 agatcatcca ccatgtggcc accatcattc tcctctgctt ctcctggttt gccaattatg
      781 tccgagcagg gaccctcatc atggctctgc atgactcttc cgactacctg ctggagtctg
      841 ccaagatgtt taactacgca ggatggaaaa acacctgcaa caacctcttc attgtcttcg
      901 ccgtcgtttt catcatcact cgactggtta tcatgccctt ctggatcctc cactgcacaa
      961 tgatataccc actggagttc taccctgcct tctttggcta ttacttcttc aattccatga
     1021 tggtagtgct acagatgctc catgtcttct gggcctactt cattgtgcgc atggcccaca
     1081 agttcataac tggaaagctg gtagaagatg aacgcagtga cagagaagaa acagagagtt
     1141 cagaggggga ggaggctgca gctggggcag gagcaaagag ccgactccta gctaatggcc
     1201 accccatcct caataacaac catcctaaga atgactgaac cgttgtccta gctgcctccc
     1261 acatcaatac gcgaagccaa gaaactagcc aagcttccct gtaggggcat ttgagctctg
     1321 gggagaaagg atatgaggag tggtctatgt tgacctccct tcttttttca cccagttgcc
     1381 tttaaaccag actctaaaca gccggtctcc aggtagaaag gaggcttggc tgtatcctgt
     1441 tgagacggga ctgtctggtc tcatttccct ctctaccttc caagctaccc tttctcctgc
     1501 taacccttct cagctctaag ggttggagtc ataactcaca ttcttcattc ctaagaatct
     1561 ggagctagct gtttgccttt gactccctga cttcaagcca gggttgtgcc ttactgtccc
     1621 ttttctgggc ctcattctgc caaagctgga ccaaggttcc acctttctaa gctccctagc
     1681 tcagaggcca aaaatgaaag ctgagctact tttaactttt tctcttcctt aactacaagt
     1741 caattaagtg aaggagggta tacatgccct caccctaccc cagggaccat ggttcctaga
     1801 tctcactgcc tcctatttct ggcctccctt ttcccctccc ttcagctaag acaagctggt
     1861 gtggagtaga agggcaacta gttctgttat ttattgaaca tttggggttt cagttgcaaa
     1921 gccagaacta cagctacctg gcatttcggc gagggaccat ttcagaccaa aatgtactgt
     1981 taattttttt taattaaagt atattaaagg ttaaataaaa caaaaaaaaa aaaa
//
