LOCUS       NM_001044230            2683 bp    mRNA    linear   ROD 20-MAR-2023
DEFINITION  Rattus norvegicus ceramide synthase 1 (Cers1), mRNA.
ACCESSION   NM_001044230
VERSION     NM_001044230.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Chen W, Wu C, Chen Y, Guo Y, Qiu L, Liu Z, Sun H, Chen S, An Z,
            Zhang Z, Li Y and Li L.
  TITLE     Downregulation of ceramide synthase 1 promotes oral cancer through
            endoplasmic reticulum stress
  JOURNAL   Int J Oral Sci 13 (1), 10 (2021)
   PUBMED   33753723
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Turpin-Nolan SM, Hammerschmidt P, Chen W, Jais A, Timper K, Awazawa
            M, Brodesser S and Bruning JC.
  TITLE     CerS1-Derived C18:0 Ceramide in Skeletal Muscle Promotes
            Obesity-Induced Insulin Resistance
  JOURNAL   Cell Rep 26 (1), 1-10 (2019)
   PUBMED   30605666
REFERENCE   3  (bases 1 to 2683)
  AUTHORS   Novgorodov SA, Riley CL, Keffler JA, Yu J, Kindy MS, Macklin WB,
            Lombard DB and Gudz TI.
  TITLE     SIRT3 Deacetylates Ceramide Synthases: IMPLICATIONS FOR
            MITOCHONDRIAL DYSFUNCTION AND BRAIN INJURY
  JOURNAL   J Biol Chem 291 (4), 1957-1973 (2016)
   PUBMED   26620563
REFERENCE   4  (bases 1 to 2683)
  AUTHORS   Obanda DN, Yu Y, Wang ZQ and Cefalu WT.
  TITLE     Modulation of sphingolipid metabolism with calorie restriction
            enhances insulin action in skeletal muscle
  JOURNAL   J Nutr Biochem 26 (7), 687-695 (2015)
   PUBMED   25771159
  REMARK    GeneRIF: Data suggest expression of enzymes involved in
            sphingolipid metabolism in skeletal muscle are regulated by dietary
            factors; caloric restriction up-regulates expression of Cers1 and
            Cers2 but down-regulates serine palmitoyltransferases (Spt1, Spt2).
REFERENCE   5  (bases 1 to 2683)
  AUTHORS   Vanni N, Fruscione F, Ferlazzo E, Striano P, Robbiano A, Traverso
            M, Sander T, Falace A, Gazzerro E, Bramanti P, Bielawski J, Fassio
            A, Minetti C, Genton P and Zara F.
  TITLE     Impairment of ceramide synthesis causes a novel progressive
            myoclonus epilepsy
  JOURNAL   Ann Neurol 76 (2), 206-212 (2014)
   PUBMED   24782409
REFERENCE   6  (bases 1 to 2683)
  AUTHORS   Spassieva S, Seo JG, Jiang JC, Bielawski J, Alvarez-Vasquez F,
            Jazwinski SM, Hannun YA and Obeid LM.
  TITLE     Necessary role for the Lag1p motif in (dihydro)ceramide synthase
            activity
  JOURNAL   J Biol Chem 281 (45), 33931-33938 (2006)
   PUBMED   16951403
REFERENCE   7  (bases 1 to 2683)
  AUTHORS   Mizutani Y, Kihara A and Igarashi Y.
  TITLE     Mammalian Lass6 and its related family members regulate synthesis
            of specific ceramides
  JOURNAL   Biochem J 390 (Pt 1), 263-271 (2005)
   PUBMED   15823095
REFERENCE   8  (bases 1 to 2683)
  AUTHORS   Duchala CS, Shick HE, Garcia J, Deweese DM, Sun X, Stewart VJ and
            Macklin WB.
  TITLE     The toppler mouse: a novel mutant exhibiting loss of Purkinje cells
  JOURNAL   J Comp Neurol 476 (2), 113-129 (2004)
   PUBMED   15248193
REFERENCE   9  (bases 1 to 2683)
  AUTHORS   Guillas I, Jiang JC, Vionnet C, Roubaty C, Uldry D, Chuard R, Wang
            J, Jazwinski SM and Conzelmann A.
  TITLE     Human homologues of LAG1 reconstitute Acyl-CoA-dependent ceramide
            synthesis in yeast
  JOURNAL   J Biol Chem 278 (39), 37083-37091 (2003)
   PUBMED   12869556
REFERENCE   10 (bases 1 to 2683)
  AUTHORS   Venkataraman K, Riebeling C, Bodennec J, Riezman H, Allegood JC,
            Sullards MC, Merrill AH Jr and Futerman AH.
  TITLE     Upstream of growth and differentiation factor 1 (uog1), a mammalian
            homolog of the yeast longevity assurance gene 1 (LAG1), regulates
            N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a
            fumonisin B1-independent manner in mammalian cells
  JOURNAL   J Biol Chem 277 (38), 35642-35649 (2002)
   PUBMED   12105227
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC166409.1.
            
            On Jul 16, 2009 this sequence version replaced NM_001044230.1.
            
            Summary: This gene encodes a member of the bone morphogenetic
            protein (BMP) family and the TGF-beta superfamily. This group of
            proteins is characterized by a polybasic proteolytic processing
            site that is cleaved to produce a mature protein containing seven
            conserved cysteine residues. The orthologous human protein has been
            shown to regulate C18-ceramide (N-stearoyl-sphinganine) synthesis.
            The protein is expressed from a bicistronic mRNA which also encodes
            growth differentiation factor 1. [provided by RefSeq, Jul 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC166409.1, DQ479969.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            bicistronic transcript :: PMID: 17364820
            NMD candidate          :: translation inferred from conservation
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2683              BC166409.1         1-2683
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p14"
     gene            1..2683
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /note="ceramide synthase 1"
                     /db_xref="GeneID:290658"
                     /db_xref="RGD:1305974"
     exon            1..277
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1081
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /EC_number="2.3.1.299"
                     /note="longevity assurance homolog 1; LAG1 longevity
                     assurance homolog 1; longevity assurance-like protein 1;
                     LAG1 homolog, ceramide synthase 1"
                     /codon_start=1
                     /product="ceramide synthase 1"
                     /protein_id="NP_001037695.1"
                     /db_xref="GeneID:290658"
                     /db_xref="RGD:1305974"
                     /translation="MAAAATTTGLEAPEPMPSYAEMLQRSWASALAAARGCGDCGWGL
                     ARRGLAEHAHLAAPELLLAVLCALGWTALRSAATTRIFRPLAKRCRLQPRDAARLPES
                     AWKLLFYLACWSYCAYLLLGTSYPFFHDPPSVFYGWRSGMAVPWDIAVAYLLQGSFYC
                     HSVYATVYMDSWRKDSVVMLVHHVVTLLLIASSYAFRYHNVGLLVFFLHDVSDVQLEF
                     TKLNIYFKARGGAYHRLHGLVANLGCLSFCFCWFWFRLYWFPLKVLYATWHCSLQSVP
                     DIPYYFFFNTLLLLLLVMNIYWFLYIVAFAAKVLTGQMRELEDLREYDSLEAQTAKPC
                     RAEKPLRNGLVKDKLF"
     exon            278..437
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /inference="alignment:Splign:2.1.0"
     exon            438..618
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /inference="alignment:Splign:2.1.0"
     exon            619..780
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /inference="alignment:Splign:2.1.0"
     exon            781..928
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1038
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1776
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..2657
                     /gene="Cers1"
                     /gene_synonym="Lass1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2657
                     /gene="Cers1"
                     /gene_synonym="Lass1"
ORIGIN      
        1 cagcggagac cgcggacgag agggtagcat ggctgctgca gcgacgacca ccgggctcga
       61 ggcgccagag cccatgccga gttatgcaga gatgttgcaa cgaagctggg cctcggcgct
      121 ggccgcagca cggggctgcg gggactgcgg ctggggactg gcgcgccgcg gcctggcgga
      181 gcacgctcat ctggctgcac ccgagctgct gctggccgtg ctctgcgctc tggggtggac
      241 agcgttgcgc tcagcagcca ccacgcgcat ctttcggccc ctggccaagc ggtgtcgcct
      301 gcagcctaga gacgcggcta ggctgcctga gagtgcctgg aagcttctgt tctacttggc
      361 ctgttggagc tactgtgcct acctgctctt gggcaccagt taccctttct tccatgaccc
      421 gccctctgtc ttctacggct ggaggtcggg catggcggtg ccctgggaca tagcggtcgc
      481 ctacctgctg caggggagtt tctactgtca ttccgtctat gccaccgtgt acatggacag
      541 ctggcgcaag gactcggtgg tcatgctggt acaccatgtg gtcaccttac tgctcatcgc
      601 ctcttcctat gcgttccgat accacaacgt gggcctcctc gtgttcttcc tgcatgacgt
      661 cagcgatgtg cagctggagt tcaccaagct caacatctac ttcaaggcca ggggtggcgc
      721 ctaccatcga ttgcacgggc tggtggccaa cctgggctgc ctcagcttct gcttctgctg
      781 gttctggttc cgcctctact ggttcccact caaggttctc tatgccacct ggcactgcag
      841 cctgcagtct gtgcctgaca ttccgtacta tttcttcttc aacactctgc tgctgctcct
      901 cctggtcatg aacatctact ggttcctgta cattgtggct tttgcagcca aggtgctgac
      961 cggtcagatg cgtgaactgg aagacttgcg ggaatacgac agtctggaag cgcagacagc
     1021 caagccctgc agagctgaga agcctctaag gaatggcctg gtgaaggaca aactcttctg
     1081 agtctcttgt cctcaactcc cgccatccag gactctgtcc cgtcctacct gggatactgg
     1141 ccccgcccct ggagactgga cccagtcccc ggaggtctgc tcccacccct ggaggcctgg
     1201 cctcgccttt ggcggcatgg ccacgcctgc aggacgatgg ccccgcccta agatgcagga
     1261 tgccaccctt ctccagggac tctggctgcc agcagctccg cctttcaggt caattctcga
     1321 ccacccacct tgggacccag cccagtcctg ccctctgggt aagtggggtc tggacacgcc
     1381 ccctccagga cctcaaagca ccctcgacct acggtcacca gcccactggt cctggacgca
     1441 gtggactctg ctgactctct tggacaccgc ctgggaggaa aatgctccct gtctgccacc
     1501 gtttttgcga ccacctcctc cttctgctct tgctgccctc aacgaccctg gcccacgcgc
     1561 cagcatcctt gggccccgct gccgccctgc tccaggttct cgggcttccc gaaacgcccc
     1621 ggagcgtccc cacactccga cctgtgcctc ctgtcatgtg gcgcctattc cgcagccgca
     1681 acccccagga ggccagagtg gggcgccctc tgaaaccatg ccacgtggag gaactggggg
     1741 ttgccggaaa cattgtgcgc cacatccccg acagcggtct gtcctccagg atcatacagc
     1801 ccgccagaac ctcagggctg tgccccgagt ggaccgtcgt ctttgacctg tcgagcgtgg
     1861 agcccataga gcgcccaaca cgcgcgcgcc tagagttgcg gctggaggct gagagcgaag
     1921 atacacgagg gtgggagcta agcgtggcgc tgttggccga ggctgagcat cctgggcctg
     1981 agctactgcg cgtgcaggcg ccacccgggc tgccactgcg cgcagaccta ctggggactg
     2041 cagtagcggc taacgcatcg gtgccgcgca ctctgcgctt ggcgctggcg ctgcaccctg
     2101 gggccgctgc aacctgtggg cgcctggctg aggcctcgtt gctgctggtg acgctggacc
     2161 cacgcctgtg tcccttgccg cggtcacggc gccacacgga gcccagggtg ggaggtggtc
     2221 cagtgggcac atgtcgcacc cgacggttgc acgtgagctt ccgtgaggtg ggctggcacc
     2281 gctgggtgat tgcgccgcgg ggctttctag ccaacttctg ccagggcacg tgtgcgctac
     2341 ctgaaacact gaggggaccc ggcgggccgc ctgcacttaa ccacgctgtg ctgcgcgcgc
     2401 tcatgcacgc agctgctccc accccgggtg taggctcgcc ctgctgcgtg ccagagcgtc
     2461 tgtcgcctat ctccgtgctc ttcttcgata atagtgacaa cgtggtgctg cgccactacg
     2521 aggacatggt ggtggatgag tgtggctgtc gctgaccacc caggacaccc tttcagggac
     2581 ggccccacgc gaaaagcagg gaccgtttgt tcatgtttta ttggtgacaa aaagcttaaa
     2641 acaaatttga ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//
