LOCUS       NM_001134861            4319 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus ceramide kinase (Cerk), mRNA.
ACCESSION   NM_001134861 XM_217019
VERSION     NM_001134861.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Hori,M., Gokita,M., Yasue,M., Honda,T., Kohama,T., Mashimo,M.,
            Nakamura,H. and Murayama,T.
  TITLE     Down-regulation of ceramide kinase via proteasome and lysosome
            pathways in PC12 cells by serum withdrawal: Its protection by nerve
            growth factor and role in exocytosis
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1867 (7), 118714 (2020)
   PUBMED   32246947
  REMARK    GeneRIF: Down-regulation of ceramide kinase via proteasome and
            lysosome pathways in PC12 cells by serum withdrawal: Its protection
            by nerve growth factor and role in exocytosis.
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Hewson,C.A., Watson,J.R., Liu,W.L. and Fidock,M.D.
  TITLE     A differential role for ceramide kinase in
            antigen/FcvarepsilonRI-mediated mast cell activation and function
  JOURNAL   Clin Exp Allergy 41 (3), 389-398 (2011)
   PUBMED   21255156
  REMARK    GeneRIF: We have identified a differential role for ceramide kinase
            in mast-cell activation
REFERENCE   3  (bases 1 to 4319)
  AUTHORS   Rovina,P., Schanzer,A., Graf,C., Mechtcheriakova,D., Jaritz,M. and
            Bornancin,F.
  TITLE     Subcellular localization of ceramide kinase and ceramide
            kinase-like protein requires interplay of their Pleckstrin Homology
            domain-containing N-terminal regions together with C-terminal
            domains
  JOURNAL   Biochim Biophys Acta 1791 (10), 1023-1030 (2009)
   PUBMED   19501188
REFERENCE   4  (bases 1 to 4319)
  AUTHORS   Graf,C., Niwa,S., Muller,M., Kinzel,B. and Bornancin,F.
  TITLE     Wild-type levels of ceramide and ceramide-1-phosphate in the retina
            of ceramide kinase-like-deficient mice
  JOURNAL   Biochem Biophys Res Commun 373 (1), 159-163 (2008)
   PUBMED   18555012
REFERENCE   5  (bases 1 to 4319)
  AUTHORS   Van Overloop,H., Gijsbers,S. and Van Veldhoven,P.P.
  TITLE     Further characterization of mammalian ceramide kinase: substrate
            delivery and (stereo)specificity, tissue distribution, and
            subcellular localization studies
  JOURNAL   J Lipid Res 47 (2), 268-283 (2006)
   PUBMED   16269826
REFERENCE   6  (bases 1 to 4319)
  AUTHORS   Mitsutake,S., Kim,T.J., Inagaki,Y., Kato,M., Yamashita,T. and
            Igarashi,Y.
  TITLE     Ceramide kinase is a mediator of calcium-dependent degranulation in
            mast cells
  JOURNAL   J Biol Chem 279 (17), 17570-17577 (2004)
   PUBMED   14769792
  REMARK    GeneRIF: Ceramide kinase has a role as a mediator of
            calcium-dependent degranulation in mast cells.
REFERENCE   7  (bases 1 to 4319)
  AUTHORS   Sugiura,M., Kono,K., Liu,H., Shimizugawa,T., Minekura,H.,
            Spiegel,S. and Kohama,T.
  TITLE     Ceramide kinase, a novel lipid kinase. Molecular cloning and
            functional characterization
  JOURNAL   J Biol Chem 277 (26), 23294-23300 (2002)
   PUBMED   11956206
REFERENCE   8  (bases 1 to 4319)
  AUTHORS   Pitson,S.M., D'andrea,R.J., Vandeleur,L., Moretti,P.A., Xia,P.,
            Gamble,J.R., Vadas,M.A. and Wattenberg,B.W.
  TITLE     Human sphingosine kinase: purification, molecular cloning and
            characterization of the native and recombinant enzymes
  JOURNAL   Biochem J 350 Pt 2 (Pt 2), 429-441 (2000)
   PUBMED   10947957
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Sep 10, 2008 this sequence version replaced XM_217019.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360186.1756304.1,
                                           SRR22582419.1777698.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-400               JAXUCZ010000007.1  119148171-119148570 c
            401-514             JAXUCZ010000007.1  119130465-119130578 c
            515-637             JAXUCZ010000007.1  119129759-119129881 c
            638-763             JAXUCZ010000007.1  119124264-119124389 c
            764-827             JAXUCZ010000007.1  119122323-119122386 c
            828-973             JAXUCZ010000007.1  119120822-119120967 c
            974-1048            JAXUCZ010000007.1  119117149-119117223 c
            1049-1201           JAXUCZ010000007.1  119115176-119115328 c
            1202-1307           JAXUCZ010000007.1  119112143-119112248 c
            1308-1384           JAXUCZ010000007.1  119111398-119111474 c
            1385-1590           JAXUCZ010000007.1  119110442-119110647 c
            1591-1799           JAXUCZ010000007.1  119109236-119109444 c
            1800-4319           JAXUCZ010000007.1  119105674-119108193 c
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..4319
                     /gene="Cerk"
                     /note="ceramide kinase"
                     /db_xref="GeneID:300129"
                     /db_xref="RGD:1305019"
     exon            1..400
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     CDS             259..1854
                     /gene="Cerk"
                     /EC_number="2.7.1.138"
                     /codon_start=1
                     /product="ceramide kinase"
                     /protein_id="NP_001128333.1"
                     /db_xref="GeneID:300129"
                     /db_xref="RGD:1305019"
                     /translation="MGAMGAAEPLQSVLWVKQQRCAVSLEPARALLRWWRSPEPGPSA
                     PDPDAYSVPVSEIIAVEEKEVHQKQSSNGRWHRMENPFAFTVHCVKRARHHRWKWARV
                     TFWSADEQLCQLWLQTLRELLENLTSRPKHLLVFINPFGGKGQGKHIYEKKVAPLFTL
                     ASITTEIIITEHANQAKETLYEINTDSYDGIVCVGGDGMFSEVLHGVIGKTQQSAGVD
                     PNHPRAVLVPSTLRIGIIPAGSTDCVCYSTVGTNDAETSALHIIIGDSLAIDVSSVHH
                     NSTLLRYSVSLLGYGFYGDLIKDSEKKRWMGLVRYDFSGLKAFLSHQYYEGTVSFLPA
                     QHTVGSPRDQKPCRAGCSVCRQSKRQLEEEQKKALYGLENAEEVEEWQVVCGKFLAIN
                     STNMSCACPRSPGGLSPFAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQEDQFGFTFV
                     EVYRVKKFQFTSKHVEDDDNDLKELEKQKFGQICKDNPPCACPTSRSSWNCDGEVLHS
                     PAIEVRVHCQLVRLFARGIEEES"
     exon            401..514
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            515..637
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            638..763
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            764..827
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            828..973
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1048
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1201
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1307
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1384
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1590
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1799
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..4319
                     /gene="Cerk"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgctcctcaa cctagggcta cagcactcga tcctgtttct gattcttctc tgtccccaag
       61 tccagcctct ccagtcctca ccctggtcgc ctcctcccgg atccccgcca ccctctctgc
      121 gtaccgggtg acgcagcacc gcctcccgct cccgcctccc cgcgccgccg ccgggcagac
      181 tgcaggggcc gctaacggtc ccgcgccccc cggcgtccgc gcgccccgcg cccggcggac
      241 gaggccgcgc cggcggcgat gggcgcaatg ggggcggcgg agccgctgca atcggtgctg
      301 tgggtgaagc agcaacgctg tgccgtcagc ttggagcccg cgcgggctct gctacgctgg
      361 tggcggagcc cggagcccgg gccctcggcg cccgatcccg atgcctactc ggtaccagtg
      421 tcagagatca tcgccgttga ggaaaaagaa gtccaccaga aacagtcctc caatggccga
      481 tggcatagaa tggaaaatcc gtttgcattc acagtccact gtgtgaagcg agctcgacac
      541 caccgctgga agtgggcacg ggtgaccttc tggagcgccg acgagcagct gtgtcaactg
      601 tggctgcaga ccctccgaga gctgctggag aacctgactt caagaccgaa gcatttgctg
      661 gtatttatca accctttcgg agggaaaggc cagggcaagc acatctatga aaaaaaagtg
      721 gcacctttgt tcaccttggc ttccatcacc actgagatca tcattactga gcatgccaat
      781 caagccaagg agactttata cgagatcaac acagacagct atgacggcat cgtgtgtgtt
      841 ggtggggacg gcatgttcag cgaggtgctg cacggggtaa ttgggaagac gcagcagagc
      901 gctggtgttg accccaatca cccccgagcc gtgctggtgc ccagtaccct aaggatcggc
      961 atcatcccgg cagggtccac agattgtgtg tgctactcaa cggtgggcac aaacgacgca
     1021 gagacgtcag ctttacacat cattattggg gactcactgg ctatagatgt gtcctccgtg
     1081 caccacaata gcacgctact gcggtactcg gtctctctgc tgggctacgg tttctacggg
     1141 gacttaatca aggacagtga aaagaaacgg tggatgggcc tcgtccggta tgatttctca
     1201 gggttgaagg cctttctctc ccatcagtac tatgaaggga cagtgtcctt cctcccagca
     1261 cagcacacag tgggatctcc acgagaccag aaaccctgcc gggctgggtg ctccgtgtgc
     1321 aggcagagca agcgacagct ggaagaggaa cagaagaaag ccctgtatgg cctggagaac
     1381 gctgaggaag tggaagagtg gcaagtggta tgtgggaagt tcctggctat caactccacc
     1441 aacatgtcct gtgcttgtcc ccggagccct gggggcctgt ccccatttgc ccatctggga
     1501 gatgggtctt ctgacctcat ccttatccgg aaatgctcca ggttcaactt cctgagattc
     1561 ctcatccggc acaccaacca ggaggatcag tttggcttca ctttcgttga agtttatcga
     1621 gtcaagaaat tccagttcac atcaaagcac gtggaagatg atgacaatga cttgaaggaa
     1681 ctagagaagc agaagtttgg gcagatctgc aaggacaacc ccccttgcgc ctgcccaacc
     1741 tctagaagct cctggaactg tgatggggaa gtcctgcaca gccctgccat tgaggtcagg
     1801 gtccactgcc agctggtgcg cctctttgct cgggggatcg aagaagagtc ctaaggaaga
     1861 atcccaaagc ccaggagctt tgggaagtgt gaatattact taagaaaatt tcgactgacc
     1921 aattatgttg acatatccac ttgaatttag aaatttattt ttgataggta aatcttggtt
     1981 tttagaaaag acagctttgt caacagttct gtatacatac ccggcatcct cggctccgcg
     2041 tgcatccacg tcgtttttca ctcgttctcg gcaaacggca ctcgctggtt tggggcactg
     2101 gcttcctaga ttttagccat gccgaccaga cgaatttcaa gacggccctt tagtggttac
     2161 actgcaataa ttttagggac atcatgacat gactccgtcc caggaatcag gttacaccat
     2221 ctggacacac gggtaatggc ccggtcactc tgacccctct ggtgagactt tctctggagt
     2281 catccatgac caggtttggg aaggcagatg gctgtctttg ccttcacttt acgcccacct
     2341 tcatttatga tcgggatgag tcgctgtgca gcccttgccg tgcaccgcaa accgtaactc
     2401 agcgaggtcg tctgcccagc cacacctgca gaacagggcc acgtcacttc gaggagacgc
     2461 ccagggtgat gtctcatcct caggatcccc acatctgctg cgttcatagc tgcgtcctgt
     2521 gagacaatcc agcaaagcac ccacttggaa gctttattcc gtcagtcttg gtagcctgca
     2581 cgccctggcc agggcagtcc tcgaagcact ggctaagagg aggctctatt tggaatcaaa
     2641 gcactttcat agctgggttt gtcggggagg cagtgcaaca tggcacacac agagggtact
     2701 ttgtgagttc gataccgccc tcaacccgac tgtcaccagc gggatggtag gtgatctcag
     2761 gtcacccctc cccccaagta ctttggtgca aactttatgt ctattcctgg gtctacatcc
     2821 attcttccca caccatggat tctgttccat gtacttattt caccgctttc tccctgtcca
     2881 tcaagaacta tgtccaacac aatccacgac ttggcaattt gctctctctc tccacaaggg
     2941 ggcgcgagct gctctggctg aagccctggg ctcatgacca gttttcctct gaaggcattc
     3001 ctgacccagg ggcttctcag ccacgcgcta tggcctctgc tgtgtctcga gacggacgca
     3061 atctgaaatg agtgtaaaag agttttatgt gaaattcaag actacgcagc ttaatcttga
     3121 cttgcaaatg ggggaagggc actgactgtt acaagagtag gttttaggtt ctgcattggg
     3181 gaaagcgtgc atcccagagg gtcctgttac tcttccacag agttccgcag gctgcacctc
     3241 tttctaggtg ctcttggact gacttatatt ttgaggcatg caggaaatcc ttctgtttag
     3301 tattcagtcc cagacagaca gaagagcagc tgcgagggtg gccatgttaa ggaaccactt
     3361 taaaggcaat ctttaaaggc ggtgctattc aatgattggt tgtcgagtgg aaagctgttt
     3421 agaaagaata aatgcaccta gcaatttcta tctagatctg tccctggatg gacagaaccc
     3481 aaaggtgtca gactagcagc tctgtgagta ggtaggcagc tctgaagcat cgtgagaaaa
     3541 aatgcttggt tggtcacctc gacgtgccca ctcacccctg gcattggaga gggggctcag
     3601 ggcccgaggc agctgtgggc gcccctggat ctcaggaact gcactctcct ccaaggattc
     3661 agatcttcat tggccttgct acctgatacc catgccctgg tccacctcct gccagttctg
     3721 gaggagagag gcagacagag ggtgctttga tggtgctttg cactgcatcc gggatctcaa
     3781 accttggcgc tatcacgggg catcatgagt gtctgacact caggaccttt gacacgggtg
     3841 tgcaggatgg gcggggaccc ctgacattcc caagtagtag acagtctcca tgatgcttct
     3901 tagatgcaat accccaacct ccccgggtct taggtatttt aggagataaa aaaaaaccca
     3961 aaaaaacaaa aaccaaacag gagtaaaggt tagacagtgg ccacatagaa tcctgtaggg
     4021 ggtgtggtcc atgttttaac tccctctgta gcccaccagt gcacaacagt taggcagcag
     4081 tcatacgcgg taacttattc ttgtttatag aaactaagcg gcagaacaag gagcaggtgg
     4141 tttcccagag gatgtttcag tcctccacgc aatctcctgg agccaggagc actgactggg
     4201 agcactgaca cgagaatggc ccaatatgcc tcccggtctt gctggcctca agattaggaa
     4261 gtggcatatt taatctgata tgtttgagaa aacaaaaaat aaagttactt tgggcagaa
//
