LOCUS       NM_001100827           10069 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus centromere protein F (Cenpf), mRNA.
ACCESSION   NM_001100827 XM_001067266 XM_223060
VERSION     NM_001100827.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Dong,W., Wang,B., Zhang,R., Cao,J. and Wu,R.
  TITLE     Therapeutic effects of acupuncture in rheumatoid arthritis are
            associated with centromere protein F expression
  JOURNAL   Allergol Immunopathol (Madr) 50 (3), 47-54 (2022)
   PUBMED   35527655
  REMARK    GeneRIF: Therapeutic effects of acupuncture in rheumatoid arthritis
            are associated with centromere protein F expression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10069)
  AUTHORS   Waters,A.M., Asfahani,R., Carroll,P., Bicknell,L., Lescai,F.,
            Bright,A., Chanudet,E., Brooks,A., Christou-Savina,S., Osman,G.,
            Walsh,P., Bacchelli,C., Chapgier,A., Vernay,B., Bader,D.M.,
            Deshpande,C., O' Sullivan,M., Ocaka,L., Stanescu,H., Stewart,H.S.,
            Hildebrandt,F., Otto,E., Johnson,C.A., Szymanska,K., Katsanis,N.,
            Davis,E., Kleta,R., Hubank,M., Doxsey,S., Jackson,A., Stupka,E.,
            Winey,M. and Beales,P.L.
  TITLE     The kinetochore protein, CENPF, is mutated in human ciliopathy and
            microcephaly phenotypes
  JOURNAL   J Med Genet 52 (3), 147-156 (2015)
   PUBMED   25564561
  REMARK    Erratum:[J Med Genet. 2016 Dec;53(12):865. doi:
            10.1136/jmedgenet-2014-102691corr1. PMID: 27738186]
REFERENCE   3  (bases 1 to 10069)
  AUTHORS   Vergnolle,M.A. and Taylor,S.S.
  TITLE     Cenp-F links kinetochores to Ndel1/Nde1/Lis1/dynein microtubule
            motor complexes
  JOURNAL   Curr Biol 17 (13), 1173-1179 (2007)
   PUBMED   17600710
REFERENCE   4  (bases 1 to 10069)
  AUTHORS   Zuccolo,M., Alves,A., Galy,V., Bolhy,S., Formstecher,E., Racine,V.,
            Sibarita,J.B., Fukagawa,T., Shiekhattar,R., Yen,T. and Doye,V.
  TITLE     The human Nup107-160 nuclear pore subcomplex contributes to proper
            kinetochore functions
  JOURNAL   EMBO J 26 (7), 1853-1864 (2007)
   PUBMED   17363900
REFERENCE   5  (bases 1 to 10069)
  AUTHORS   Bomont,P., Maddox,P., Shah,J.V., Desai,A.B. and Cleveland,D.W.
  TITLE     Unstable microtubule capture at kinetochores depleted of the
            centromere-associated protein CENP-F
  JOURNAL   EMBO J 24 (22), 3927-3939 (2005)
   PUBMED   16252009
REFERENCE   6  (bases 1 to 10069)
  AUTHORS   Chan,G.K., Schaar,B.T. and Yen,T.J.
  TITLE     Characterization of the kinetochore binding domain of CENP-E
            reveals interactions with the kinetochore proteins CENP-F and
            hBUBR1
  JOURNAL   J Cell Biol 143 (1), 49-63 (1998)
   PUBMED   9763420
REFERENCE   7  (bases 1 to 10069)
  AUTHORS   Zhu,X., Mancini,M.A., Chang,K.H., Liu,C.Y., Chen,C.F., Shan,B.,
            Jones,D., Yang-Feng,T.L. and Lee,W.H.
  TITLE     Characterization of a novel 350-kilodalton nuclear phosphoprotein
            that is specifically involved in mitotic-phase progression
  JOURNAL   Mol Cell Biol 15 (9), 5017-5029 (1995)
   PUBMED   7651420
REFERENCE   8  (bases 1 to 10069)
  AUTHORS   Zhu,X., Chang,K.H., He,D., Mancini,M.A., Brinkley,W.R. and Lee,W.H.
  TITLE     The C terminus of mitosin is essential for its nuclear
            localization, centromere/kinetochore targeting, and dimerization
  JOURNAL   J Biol Chem 270 (33), 19545-19550 (1995)
   PUBMED   7642639
REFERENCE   9  (bases 1 to 10069)
  AUTHORS   Liao,H., Winkfein,R.J., Mack,G., Rattner,J.B. and Yen,T.J.
  TITLE     CENP-F is a protein of the nuclear matrix that assembles onto
            kinetochores at late G2 and is rapidly degraded after mitosis
  JOURNAL   J Cell Biol 130 (3), 507-518 (1995)
   PUBMED   7542657
REFERENCE   10 (bases 1 to 10069)
  AUTHORS   Rattner,J.B., Rao,A., Fritzler,M.J., Valencia,D.W. and Yen,T.J.
  TITLE     CENP-F is a .ca 400 kDa kinetochore protein that exhibits a
            cell-cycle dependent localization
  JOURNAL   Cell Motil Cytoskeleton 26 (3), 214-226 (1993)
   PUBMED   7904902
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000013.1, DY549432.1 and EV780064.1.
            
            [WARNING] On Oct 10, 2024 this sequence was replaced by
            NM_001100827.2.
            
            On or before Dec 19, 2009 this sequence version replaced
            XM_001067266.1, XM_223060.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMEA5760383, SAMEA5760386
                              [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               JAXUCZ010000013.1  103760777-103760886 c
            111-304             JAXUCZ010000013.1  103757497-103757690 c
            305-501             JAXUCZ010000013.1  103756318-103756514 c
            502-614             JAXUCZ010000013.1  103755279-103755391 c
            615-706             JAXUCZ010000013.1  103754816-103754907 c
            707-992             JAXUCZ010000013.1  103752617-103752902 c
            993-1195            JAXUCZ010000013.1  103751341-103751543 c
            1196-1321           JAXUCZ010000013.1  103744741-103744866 c
            1322-1450           JAXUCZ010000013.1  103743205-103743333 c
            1451-1573           JAXUCZ010000013.1  103740794-103740916 c
            1574-1709           JAXUCZ010000013.1  103734648-103734783 c
            1710-5092           JAXUCZ010000013.1  103729464-103732846 c
            5093-7327           JAXUCZ010000013.1  103725724-103727958 c
            7328-7378           DY549432.1         510-560
            7379-7927           EV780064.1         1-549
            7928-8080           JAXUCZ010000013.1  103723906-103724058 c
            8081-8240           JAXUCZ010000013.1  103723328-103723487 c
            8241-8717           JAXUCZ010000013.1  103721933-103722409 c
            8718-8896           JAXUCZ010000013.1  103720044-103720222 c
            8897-10069          JAXUCZ010000013.1  103715344-103716516 c
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q26"
     gene            1..10069
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /note="centromere protein F"
                     /db_xref="GeneID:257649"
                     /db_xref="RGD:628667"
     exon            1..110
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            111..304
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..9100
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /note="LEK/centromere protein; centromere autoantigen F"
                     /codon_start=1
                     /product="centromere protein F"
                     /protein_id="NP_001094297.1"
                     /db_xref="GeneID:257649"
                     /db_xref="RGD:628667"
                     /translation="MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSL
                     EAALQKQKQKVEDGKTEGADLKRENQRLMEICENLEKTRQKLSHELQVKESQVNFQES
                     QLSSCKKQIEKLEQELKRCKSEFERSQQSAEVSLNPCSTPQKLFTTPLTPSQYYICST
                     YEDLKEKYNKEVEDRKRLEAEVKALHAKKASLPVSQATMNHRDIARHQASSSVFSWQQ
                     DKTPSRLSSCSLRTPLRRDVSAAHFLGEEVTPNKSSVQIGRGDCSGLPDDPHCSQPLH
                     QAKAQNQELKSKMNELELRLRGQEKEMKDQVNKFQELQLQLEKTKVDLIEKEKILNKT
                     RDEVVRTTAQYEQAAAKCTALEQKLKNLTEELSCHRQNAESAKRSLEQRVKEKEKELR
                     EELSRQHQSFQALDHEYTQMKTRLTQELQQAKHSLSVLQLELEKVTSVKQQLERSLEE
                     IRHKFSRAEQALQASQLTENELRRSSEEMKKENSLIRSQSEQRTREACRLEDELGKVK
                     VCLSQSQSFAEEMRAKNTSQEIMLRDLQEKLNQQENSLTLEKLKLALADLEGQRDCSQ
                     DLLKKREHHIEQLNDKLNKIEKEFETLLSALELKKKECEELKEEKNQISCWKSENEKL
                     INQIESEKEILLGKVNHLEASLKTQQISHDYSERVRTLEMERENLTVEIRNLHSLLDS
                     KMVEIETQKQAYLELQQQSESSDQKHQKEMENMCLKANKLTGQVESLECKLQSLSGEV
                     ETKDQQYQDLRMEYETMRDSLQARGSSLVTDEKNQRSSSAFEEQPAVSHSFANLVGEK
                     GSIYSERSDSSVDRGQSPENVAVLQSRVTLLESSLESQNQMNSDLQKQCEELLQIKGE
                     IEENLIKADQIHQNFVAETNQRIGKLQEDAAVHQNIVAETLATLESREKELQLLKEQL
                     EAQQTEVQKLEKNNCLLEGALKELQLLTDTLSSEKKEMNSIISLRKKDIEELTQANGA
                     LKEVNEALRQEKMNLLQQHEEITRCVAEGERSIAELSGQYKQERLLLLQRCEETETVL
                     EGLRGDYKAAQENNAKLECMLSECTALCENKKNELEQLKETFAKEQQEFLTKLAFTEE
                     QNRKLVLELETEPQTVRSEITNINKHPVSETDALGQESWSSKEEQKEKQKEVSNLTPE
                     NEQLMELTQTKHDYYHLEVEPVENSVKATEEEIRKSSSQYQMDIDTKDISLDSYKAQL
                     VQLEALIKVMEVKLDRSEEEKNSLRQELQTIREELGTKTSQDTQSQARVGLKDCEVEA
                     EEKYVSVLQELSTSQNENVHLQCSLQTAMNKLNELGKMCEVLRVEKLQLESELNDSRS
                     ECITATSQMAAEVEKLVNEMKMLNHENALSHGELMKDTADVEFDDKPNHTSVFLTPLD
                     NSEQMTSSNKEVRVHFAELQEKFSCLQSEHKILHDQHCEVSSKMSALRSYVDTLKAEN
                     SVLSMNLRTLQGDLVKEKEPGAEDGHILPLSFCRTDSPTLTNFGENSFYKDVLEQTGD
                     TSHLSLEGNASANPCDVDEVSYSSLEEENLTEKEIPFASLRTVEELEILCQVYLQSIK
                     NLEEKIESQRIMKNKEIEELEQLLSSERKELSCLRKQYLSEKEQWQQKLTSVTLEMES
                     KLAEEKQQTKNLSLELEVARLQLQELDLSSRSLLGTDLEGAVRGPNDGYAIKESEVYI
                     SETTEKTPKQDTNQTCDNDVQQDLCLETSVTETETTRLTGDGCEEQPSKISCEAPAED
                     RTQDYSECISELFSTPSVLVPMDVLEDQGSIQNLHLQKDTSNENLRLLPEVEDWDKKV
                     ESLLNEIKEADSKLSLQELQLKIKIATCIELEKIVKHLKKEETDLSEKLESLPCNQEV
                     CPRVERSDLDFNLDMGDDELFRESTKDDAANTEDNYKEKFLDMERELTRIKSEKANIE
                     HHIVSVKANLEVVQAEKLCLERDTESKQKVIVDLKGELFTVISERNRLREELDNVSKE
                     SKALDQMSKKMKEKIEELESHQRESLHHIGAVESEVKDKAELIQTLSFSVDELTKDKA
                     HLQEQLQNLQNDSQGLSLAIGELEIQIGQLNKEKESLVKESQNFQVKLTESECEKQTI
                     SKALEVALMEKGEFAMRLSSTQEEVHQLRQGIEKLSVRIEADEKKHLSAVAKLKESQR
                     ESDSLKDKVENLERELEMSEENQELAILDSENLKAEVETLKAQMDEMAKSLRVFELDL
                     VNVRSERENLAKQLQEKQSRVSELDELCSSLRSLSEEKEQARVQMERDSKSAMLMLQT
                     QLKELWEEVAALYNDQETLKAQEQSLDQPGEEVHLLKSSIQKLKVHIDADKKKQCHIL
                     EQLKESKHHADLFKDRVENLEQELMLSEKNKEHLIFQAENSKAEIQTLKTEIQTMDQN
                     LQDLELELTNTRSEKENLMKELKNEQEQISKLETINSSIERLLTDKEQEKVQVKEEAR
                     ITVEMLQTQLKELNETVVSLCNDQEAYKTKEQNLGSRVQTLELEKAQLLQDLGEAKNK
                     YIIFQSSVNALTQEVEAGKQKLEKEEEEVRTLKEQLKGQEQLVCKLARVEGEQQLCQK
                     QKLELRSLTMELEQKVKVLQSENDALQTTYEALQSSYKSLEGELGLIKMEKMALVERV
                     NTMTGKEAELQRELHDVEQKSTQLKEEYSKEKTRLTEDLEVVMEELKNTKVAHLKNVN
                     HLEKEFQRAQGKIKLLLKSCKQLEGEKKMLQKELSQLEAAQKQRAGSLVDSNVDELMT
                     ENKELKETLEEKIKEADKYLDKYCSLLISHEELEKTKEILEIQVARLSSRQNKQDLQS
                     SPLLDSSVAGPSPSTSVSERKSTSGLNKTLGKRQRSSGIGENGNGTAPSTPETFSKKS
                     RKSVSNSAHPAEDEEETEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRL
                     AAQKLLGSSPTLGKENLVESSKPTAGGSRSQKVKVVQESSMDAHAVFQELPAKSLTAN
                     NMPGRNSTESPREGLRAKRACPASSPAAGPDPMNNENCRVQ"
     exon            305..501
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            502..614
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            615..706
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            707..992
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1195
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1321
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1450
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1573
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1709
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..5092
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            5093..7927
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            7928..8080
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            8081..8240
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            8241..8717
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            8718..8896
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
     exon            8897..10069
                     /gene="Cenpf"
                     /gene_synonym="Lek1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtttgaatc gctcgtgctg ggcggcaggt gcggcgcgct ttgtgaggag ctcggcggtg
       61 aagaacccgg ggttctcgga tccctgagcc gggttccgag aggcgctcag tttacttcta
      121 gatgctttaa aaataaaaca agatgagctg ggcgttggaa gagtggaagg aaggtctccc
      181 gaccagagct cttcagaaaa ttcaagagct tgaaggacag ctggacaaac tgaagaagga
      241 gaaacagcag aggcagtttc agctggattc tctcgaagcc gcgctgcaga agcagaagca
      301 gaaggttgaa gatggaaaaa ctgagggtgc agacctgaaa agggaaaatc aaagattgat
      361 ggagatatgt gaaaatttgg agaaaacacg gcagaagctt tctcacgaac ttcaagttaa
      421 ggagtcacaa gtgaattttc aagaaagtca actgagctca tgcaaaaagc aaatagaaaa
      481 gctggagcag gagcttaagc ggtgtaaatc tgagtttgag agaagccaac aatcggcaga
      541 ggtttcctta aacccatgca gtacacccca gaaactcttc accactccac tcacaccaag
      601 tcagtattat atctgttcca cgtatgaaga tctaaaagaa aaatataata aagaagttga
      661 agaccggaaa agattagagg cagaggttaa agctttgcat gcgaaaaaag cgagcctgcc
      721 tgtttcccaa gccaccatga atcaccggga cattgcaaga catcaggctt catcatcggt
      781 attttcgtgg caacaggaca aaaccccaag tcgcctttca tcttgttctc tgagaacccc
      841 actacggaga gatgtctctg ctgctcactt tttgggggaa gaagtgaccc ctaacaaatc
      901 aagtgttcag atagggagag gagactgcag tggcctccct gacgatcctc attgctctca
      961 gcctttgcat caggccaaag cccagaatca agaactaaaa agcaagatga atgagttaga
     1021 actacgcctg cgaggacaag aaaaggaaat gaaagaccaa gtgaataaat tccaagagtt
     1081 acagctacaa ctggagaaaa caaaagtgga cttaattgaa aaagagaaaa ttttgaataa
     1141 aaccagagat gaagtagtga gaaccacagc ccagtatgag caagcagcag ccaagtgtac
     1201 tgccttggaa caaaagctga agaatttgac ggaggagttg agttgtcatc gacagaacgc
     1261 agagagtgct aaacgttctc tggaacagag agttaaggag aaagaaaagg aacttcgaga
     1321 ggagctgtcc cgacaacatc aatctttcca agctctggac catgagtaca ctcagatgaa
     1381 aaccagactt acccaggagt tacagcaagc caagcattcg ctcagcgtcc tccagctgga
     1441 gctggagaag gtcacatcag tgaagcagca gttagaaagg agtttggaag aaattaggca
     1501 caagttcagc agagcagaac aagctcttca ggcgagtcag ctcacagaga atgagctgag
     1561 gagaagcagt gaggaaatga agaaggaaaa cagtctcatt aggagtcagt ctgagcaaag
     1621 gaccagggaa gcctgccgcc tggaggacga acttggtaaa gtcaaagtgt gtttgagtca
     1681 gagccagagt tttgcagaag aaatgagagc taagaatacc tctcaggaaa tcatgttacg
     1741 agatcttcag gaaaaactaa atcagcaaga aaactcacta actttagaaa aactgaaact
     1801 tgccttagct gacctggaag gacagcgaga ctgttctcaa gatcttttga agaaaaggga
     1861 acatcacatt gagcaactga atgataagtt aaataagata gagaaggaat ttgaaacttt
     1921 gctgagtgct ttagaattaa aaaagaaaga atgtgaagaa ttgaaagaag agaaaaatca
     1981 gatttcttgt tggaaaagtg aaaatgaaaa actcataaat cagatagagt cagaaaaaga
     2041 aatcttactg ggtaaagtca accacttaga ggccagcctc aagacacaac aaataagtca
     2101 tgactatagt gagagagtaa ggacactaga gatggaaaga gaaaacctta ctgtggagat
     2161 tagaaacctc catagtcttt tagacagcaa gatggtggag atcgagacac agaaacaagc
     2221 ctacttggaa ctgcagcagc aatctgaatc ctcagaccag aagcatcaga aagaaatgga
     2281 gaatatgtgc ttgaaagcta ataagctcac tgggcaagtt gaaagtttgg agtgtaagct
     2341 gcagtcactg tcaggggaag tagaaaccaa agaccagcag taccaagact tgcgtatgga
     2401 gtacgagaca atgagggatt cgctccaggc cagaggatct tctctggtga cggatgagaa
     2461 gaatcagaga agctcttcag cttttgaaga gcagcctgca gtgagtcatt cctttgcaaa
     2521 cctagtgggg gaaaaaggaa gcatatattc agaaaggagt gacagctctg tagatagggg
     2581 ccaaagtcca gaaaatgtag ctgtcttaca aagtagagtc actttacttg aaagttcctt
     2641 ggagtctcaa aaccagatga actcagattt gcaaaagcag tgtgaagagt tgctacaaat
     2701 caaaggggaa attgaggaaa acctcattaa agcagaccag attcatcaaa actttgtggc
     2761 tgaaacaaat caacgtattg gtaaattgca ggaagatgct gcagttcatc agaatattgt
     2821 tgctgaaact ctagcaactc ttgagagtag ggaaaaagag ttacagcttt tgaaagaaca
     2881 attagaagcc cagcaaacag aggttcaaaa gttagagaag aataactgtc ttcttgaagg
     2941 tgctctgaag gagctacagc ttttaactga cactctgagc tccgagaaga aagaaatgaa
     3001 ttctatcatc tcattaagga aaaaagacat tgaagagtta acccaagcaa atggggctct
     3061 caaagaagtt aatgaggcct tacggcagga gaaaatgaat cttctccaac agcatgagga
     3121 gattacaagg tgtgtagcag aaggagagag aagcattgca gagttgtctg gtcagtacaa
     3181 gcaagaaaga cttttgttat tacaacggtg tgaagaaaca gaaactgtgt tggaaggtct
     3241 cagaggagac tacaaagcag cacaagaaaa caatgctaag ttagaatgca tgctcagtga
     3301 gtgcactgct ctttgtgaga ataaaaaaaa tgaactagag cagttaaagg aaacatttgc
     3361 aaaggaacag caagaattct taacaaaatt agctttcact gaagagcaaa acaggaagtt
     3421 agtgctagag ttggagacag agccacaaac tgtgagatct gagattacaa acatcaacaa
     3481 gcatcccgtg agtgagactg atgccttagg gcaagaaagc tggtcttcaa aggaagagca
     3541 aaaggagaag caaaaggaag tcagcaactt aacacctgag aacgagcaat tgatggagtt
     3601 aacacagact aaacatgatt actatcatct tgaagtagag ccagttgaga actctgtaaa
     3661 agcaacagaa gaagagataa ggaagagtag ttcccagtat cagatggata ttgacactaa
     3721 agacatttct ctagacagtt ataaagcaca gttggtacaa ctagaagctt tgataaaagt
     3781 tatggaagta aaactcgacc gaagtgagga ggagaaaaac agcctgcgtc aggaattaca
     3841 gacaattaga gaagagctgg gaaccaagac ttcacaggac actcagtcac aggcgagggt
     3901 tggccttaaa gactgtgaag tagaagcaga ggaaaagtat gtgtcagtgc tccaggagtt
     3961 gtcaacaagt caaaatgaga atgtgcactt acagtgctct ctgcagaccg caatgaacaa
     4021 attgaatgaa ctagggaaaa tgtgtgaagt actgagagtt gaaaagttac aactagagtc
     4081 tgagctgaat gactcacgat ctgagtgtat cacagcaact agtcaaatgg cggcagaggt
     4141 cgagaagcta gtgaatgaaa tgaaaatgct caaccatgag aatgctctgt cccacggtga
     4201 gctgatgaaa gatactgcag atgttgaatt tgatgataaa ccaaaccaca catctgtgtt
     4261 cttgactcct ttggacaata gtgaacaaat gacctcatca aacaaagagg tccgagtaca
     4321 ctttgctgaa ttacaggaga aattctcctg tttacaaagt gaacacaaaa ttttacatga
     4381 tcagcactgt gaggtgagct ctaagatgtc agcactgcgt tcctatgttg acacattaaa
     4441 agctgaaaat tctgtcttgt caatgaatct gagaactttg cagggtgact tggtaaagga
     4501 gaaggagcct ggagctgagg atggacatat tctgccactg tcattctgta ggacagacag
     4561 ccctactctg acaaattttg gagaaaattc cttttataaa gatgttttag aacaaactgg
     4621 agacacatct catttaagtt tagaagggaa tgcttcagca aatccttgtg atgtagatga
     4681 agtgtcttac agtagtctgg aggaggagaa tctgactgag aaagaaatcc catttgcctc
     4741 tctgaggact gttgaagaac ttgaaatcct gtgtcaggtg tacttgcagt ccatcaagaa
     4801 tctagaagag aaaattgaga gtcaaaggat tatgaaaaat aaagaaattg aagagcttga
     4861 gcagttacta agttctgaga ggaaggaact aagctgtctt aggaagcaat atttgtcaga
     4921 aaaggaacaa tggcagcaga agctgacaag tgtcactttg gaaatggagt ccaagttagc
     4981 agaagagaag cagcagacca agaatttgtc ccttgaactt gaggtagcac gacttcagtt
     5041 gcaggagctg gacttaagct caaggtcttt gcttggcact gacttggaag gtgctgttcg
     5101 gggcccaaat gatggttatg ctataaaaga atcagaagta tatatctcag agactacaga
     5161 gaaaacgcca aagcaggaca ctaatcagac ttgtgataac gatgttcagc aggacctttg
     5221 tctagaaact tcagtcactg agaccgagac taccaggctc acaggagatg ggtgtgaaga
     5281 gcagccttca aagatcagtt gtgaggcacc agcagaggac agaacccaag actattcaga
     5341 atgcatttct gaattgtttt ctactcccag tgttttagta cccatggatg ttctggaaga
     5401 tcaggggtct atccagaatc tccatttgca gaaagataca tcaaatgaga atttgagatt
     5461 acttcctgaa gtagaagact gggacaaaaa agttgaaagt ttgctgaatg aaattaagga
     5521 ggcagattca aaactcagtt tacaggaact gcagctaaag atcaagattg caacatgtat
     5581 agaattggaa aaaatagtca agcacctcaa gaaggaagaa actgacttaa gtgaaaaatt
     5641 ggaatctctt ccgtgtaacc aggaggtatg tccaagagta gaaaggtcag atcttgattt
     5701 taacttagat atgggagacg atgagttgtt tcgtgaatct actaaagatg atgcagccaa
     5761 cacagaagac aattacaagg agaagtttct tgatatggaa agagaactga ccaggattaa
     5821 gtctgagaaa gctaatattg agcatcacat tgtatctgtg aaagctaact tagaggtagt
     5881 tcaagcagag aagctctgtt tagaaagaga cactgaaagt aagcagaagg ttattgttga
     5941 ccttaaagga gaactattta cagttataag tgagagaaac agacttcgtg aagaactaga
     6001 taatgtgtca aaagaaagca aagcactgga ccagatgtct aaaaagatga aggagaaaat
     6061 agaagagctg gagtctcacc agagggagag cctccatcac attggggcag tagagtctga
     6121 ggtcaaggac aaagcagaac tcattcagac tctgtccttc agtgtggatg agctaacaaa
     6181 agacaaagct catctccagg agcagctgca gaatttgcag aatgactcac agggattgtc
     6241 tttggcaatt ggtgagctgg aaatccagat tggacaactg aataaagaga aagaatcact
     6301 agtcaaggag tctcaaaact tccaggtcaa gctgactgag tcggaatgtg aaaagcagac
     6361 catttctaag gccttggagg tggcactcat ggagaaaggt gagtttgcaa tgcgactgag
     6421 ctcaacccag gaggaggtgc atcagctgag acaaggcatt gagaaactga gtgtccgaat
     6481 tgaggcagat gaaaagaagc acctcagtgc tgtggcgaaa ctgaaagaaa gccagcggga
     6541 aagtgactca ttgaaggata aagtggaaaa tctggagagg gagttggaga tgtcagaaga
     6601 aaaccaagag ctggcgattc ttgattctga gaatttgaaa gcagaggtgg agaccctcaa
     6661 ggcacaaatg gatgaaatgg ccaaaagcct gagagttttt gaattagacc ttgtcaatgt
     6721 tagatctgaa agagaaaatc tagcaaagca gctgcaagag aaacaaagtc gagtgtcaga
     6781 attagatgaa ctgtgctctt ccttgagaag tctgtcagaa gagaaggagc aagcaagagt
     6841 acagatggaa agagactcta aatcagcaat gctgatgctt cagacacagt tgaaagagct
     6901 ctgggaggaa gtagcagcct tgtataatga ccaagaaacc ttaaaggccc aagaacagag
     6961 tctagaccaa ccaggggaag aagtgcatct cttgaaaagt agtattcaaa aactaaaagt
     7021 tcacatagat gctgacaaaa aaaagcagtg ccacatccta gaacagctga aggaaagtaa
     7081 gcatcacgca gacttgttta aggacagagt tgagaacctt gaacaagaat tgatgctatc
     7141 cgagaaaaac aaagaacact tgattttcca agctgaaaat tccaaagcag agatacagac
     7201 tttaaaaaca gaaattcaaa caatggacca aaacctccaa gatttggagt tagaacttac
     7261 taatacaagg tcagaaaagg aaaatctcat gaaagaacta aaaaatgagc aagagcaaat
     7321 atctaaatta gaaacaataa attcttctat tgaaagatta ctgacagata aagagcaaga
     7381 aaaagtacag gtgaaagagg aagccagaat tacagtggag atgcttcaaa ctcagttaaa
     7441 ggagctaaat gagacagtgg tttccttgtg caatgaccag gaggcctata agaccaaaga
     7501 gcagaatctg gggagccgag tacaaactct tgaacttgag aaggctcagc tgctacaaga
     7561 ccttggtgag gccaagaata aatacattat ctttcagtca tctgtaaatg ccctcactca
     7621 agaagtagaa gctggcaaac agaaactaga gaaggaggag gaagaggtca ggacactgaa
     7681 agaacaactt aaaggtcaag agcagcttgt gtgtaaactc gcccgagtgg aaggagagca
     7741 gcaactctgt cagaagcaga aactagagct gagaagtctg actatggagc tggagcagaa
     7801 ggtcaaagtg ctgcagtctg aaaacgacgc gctgcagacc acctacgaag cactgcagag
     7861 ttcctacaag agtttagaag gtgaacttgg gttgataaag atggagaaaa tggcgctggt
     7921 ggaaagagtt aacacaatga ctgggaagga agcagagcta cagagggagc tgcatgacgt
     7981 ggaacagaaa agtacgcagc tgaaagaaga gtacagcaag gagaaaacca ggctaacgga
     8041 agacttagag gtggtgatgg aagagctgaa gaacaccaaa gtagcacacc ttaaaaatgt
     8101 gaatcatctt gagaaggaat ttcagcgtgc tcaggggaaa ataaaattgc tgctcaaatc
     8161 ctgtaaacag ctggaaggag aaaagaaaat gttgcagaaa gagctctcgc agcttgaagc
     8221 tgcacagaag cagagagcag gttctcttgt ggatagtaat gtggatgaat taatgactga
     8281 gaacaaagag ctgaaagaga ctcttgaaga aaaaatcaag gaagcagata agtacttgga
     8341 taagtactgt tcccttctta taagccatga ggagctagag aaaaccaaag agatattaga
     8401 aatacaagtc gctcgactca gttcacggca gaacaaacag gatctccaga gttctccttt
     8461 gctggattct tcggttgcag gaccatctcc aagtacttct gtcagcgaga ggaagtcaac
     8521 atctggccta aataaaactt taggcaagag gcaaaggtcc agtggaattg gggagaacgg
     8581 gaatgggaca gcaccttcta caccagagac gttctctaag aaaagcagga aatctgtcag
     8641 taatagtgct caccctgctg aggacgagga agagactgag tttgagccag aaggactccc
     8701 agaagttgtt aaaaaagggt ttgctgacat cccgactgga aagacaagcc catatatcct
     8761 tcggagaaca actatggcaa ccagaacaag cccccgcctt gctgcacaga agttattggg
     8821 atcttcccca actctgggca aagaaaatct tgtagagtcc tccaaaccaa cagctggtgg
     8881 cagcagatca caaaaggtca aggttgttca ggagagctca atggatgcac atgctgtctt
     8941 ccaggaactc ccagcaaaat ctctcacagc caataatatg cctgggagaa actctacaga
     9001 gagccccagg gagggcctga gggccaagcg ggcctgccct gcctccagcc cagccgctgg
     9061 gcctgacccc atgaacaatg aaaactgccg agtccagtga gacgtctact cagcattctg
     9121 gaaggtggca gaactcagag gaacctgtct gtgaggactt gtcttaagcc aaggacatgt
     9181 tatctgtctc tagagaatac ctgctgtttg tgtggtgttc agtctcccat atgtcagacg
     9241 caaacgtttg aaagaaccta ctaccctagc ccttcttctt cttccttccc tacccctaat
     9301 agtcctgatt cctaaccttg agtgtaaata gtggagagct ggagaattgg ctgcagttaa
     9361 atgggaagca ccataacgat ttgtgtgtgg actttacact ccatgctgca gtgattggga
     9421 tcttcctgtt tagaacgtta ggcatcagat cactgtagat caagaattgg agctgagtta
     9481 agtgaggaga gcctgtgtgc atgtggtgtg gccctctcct cagtgtgttt cataacctcc
     9541 tgctcacgta acctgtgcgt accatgtggg gagtgcagtg agcactcagg cctgcagagt
     9601 aggactgtac agacagacac cttagtggtg gtgtagatga tgtcaactgt cactgctgtc
     9661 ctgctaaagg catcatagtt cttttggtat atatttctgt gtctggtgat ttattgaagt
     9721 atgttctttt ttgcaaaagt tatttcctaa atataaaaac attatgacta gcaccactgt
     9781 gtcatgtttt cttaactaaa accacctctc ataaaagaaa acatgtatga acaaatgata
     9841 cagttttttg aatgtgtgac actagtatac tcactgaacg tgagacatac tggaatgttg
     9901 gtcatacaga tggtttgaga atagggatgt tttaaattct gatgtcttgt gctcattaaa
     9961 tatcactagc aaaggtacaa gagtgaagtt atcccatctg tctgtggata atagggcttg
    10021 aagagatgac ggcattttga cccatatcaa ataaaaacac ttgactgag
//
