LOCUS       NM_012553                908 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus chymotrypsin like elastase 2A (Cela2a), mRNA.
ACCESSION   NM_012553
VERSION     NM_012553.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Esteghamat,F., Broughton,J.S., Smith,E., Cardone,R., Tyagi,T.,
            Guerra,M., Szabo,A., Ugwu,N., Mani,M.V., Azari,B., Kayingo,G.,
            Chung,S., Fathzadeh,M., Weiss,E., Bender,J., Mane,S., Lifton,R.P.,
            Adeniran,A., Nathanson,M.H., Gorelick,F.S., Hwa,J., Sahin-Toth,M.,
            Belfort-DeAguiar,R., Kibbey,R.G. and Mani,A.
  TITLE     CELA2A mutations predispose to early-onset atherosclerosis and
            metabolic syndrome and affect plasma insulin and platelet
            activation
  JOURNAL   Nat Genet 51 (8), 1233-1243 (2019)
   PUBMED   31358993
REFERENCE   2  (bases 1 to 908)
  AUTHORS   Becari,C., Teixeira,F.R., Oliveira,E.B. and Salgado,M.C.
  TITLE     Angiotensin-converting enzyme inhibition augments the expression of
            rat elastase-2, an angiotensin II-forming enzyme
  JOURNAL   Am J Physiol Heart Circ Physiol 301 (2), H565-H570 (2011)
   PUBMED   21602471
  REMARK    GeneRIF: Suggest that elastase-2 may contribute as an alternative
            pathway for ANG II generation when ACE is inhibited in the rat
            vascular tissue.
REFERENCE   3  (bases 1 to 908)
  AUTHORS   Bonnart,C., Deraison,C., Lacroix,M., Uchida,Y., Besson,C.,
            Robin,A., Briot,A., Gonthier,M., Lamant,L., Dubus,P., Monsarrat,B.
            and Hovnanian,A.
  TITLE     Elastase 2 is expressed in human and mouse epidermis and impairs
            skin barrier function in Netherton syndrome through filaggrin and
            lipid misprocessing
  JOURNAL   J Clin Invest 120 (3), 871-882 (2010)
   PUBMED   20179351
REFERENCE   4  (bases 1 to 908)
  AUTHORS   Santos,C.F., Greene,A.S., Salgado,M.C. and Oliveira,E.B.
  TITLE     Conversion of renin substrate tetradecapeptide to angiotensin II by
            rat MAB elastase-2
  JOURNAL   Can J Physiol Pharmacol 82 (11), 1000-1005 (2004)
   PUBMED   15644940
REFERENCE   5  (bases 1 to 908)
  AUTHORS   Santos,C.F., Caprio,M.A., Oliveira,E.B., Salgado,M.C.,
            Schippers,D.N., Munzenmaier,D.H. and Greene,A.S.
  TITLE     Functional role, cellular source, and tissue distribution of rat
            elastase-2, an angiotensin II-forming enzyme
  JOURNAL   Am J Physiol Heart Circ Physiol 285 (2), H775-H783 (2003)
   PUBMED   12714330
  REMARK    GeneRIF: the fact that cultured mesenteric arterial bed endothelial
            cells are capable of producing and secreting elastase-2 represent
            strong evidence of a physiological role for this enzyme in the rat
            vasculature.
REFERENCE   6  (bases 1 to 908)
  AUTHORS   Santos,C.F., Oliveira,E.B., Salgado,M.C. and Greene,A.S.
  TITLE     Molecular cloning and sequencing of the cDNA for rat mesenteric
            arterial bed elastase-2, an angiotensin II-forming enzyme
  JOURNAL   J Cardiovasc Pharmacol 39 (5), 628-635 (2002)
   PUBMED   11973405
  REMARK    GeneRIF: Analysis of rat mesenteric arterial bed elastase-2,
            expressed in mesenteric arteries and lung but not in aorta, reveals
            primary structural features that could contribute to the lack of
            activity of this enzyme toward angiotensin II.
REFERENCE   7  (bases 1 to 908)
  AUTHORS   Swift,G.H., Craik,C.S., Stary,S.J., Quinto,C., Lahaie,R.G.,
            Rutter,W.J. and MacDonald,R.J.
  TITLE     Structure of the two related elastase genes expressed in the rat
            pancreas
  JOURNAL   J Biol Chem 259 (22), 14271-14278 (1984)
   PUBMED   6094548
REFERENCE   8  (bases 1 to 908)
  AUTHORS   MacDonald,R.J., Swift,G.H., Quinto,C., Swain,W., Pictet,R.L.,
            Nikovits,W. and Rutter,W.J.
  TITLE     Primary structure of two distinct rat pancreatic preproelastases
            determined by sequence analysis of the complete cloned messenger
            ribonucleic acid sequences
  JOURNAL   Biochemistry 21 (6), 1453-1463 (1982)
   PUBMED   6918221
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from V01233.1.
            
            On Feb 21, 2013 this sequence version replaced NM_012553.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: V01233.1, FQ226773.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-908               V01233.1           1-908
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..908
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /note="chymotrypsin like elastase 2A"
                     /db_xref="GeneID:24332"
                     /db_xref="RGD:2548"
     exon            1..61
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..837
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /EC_number="3.4.21.71"
                     /note="elastase-2; elastase-2A; Elastase 2, pancreatic;
                     chymotrypsin-like elastase family, member 2A"
                     /codon_start=1
                     /product="chymotrypsin-like elastase family member 2A
                     precursor"
                     /protein_id="NP_036685.1"
                     /db_xref="GeneID:24332"
                     /db_xref="RGD:2548"
                     /translation="MIRTLLLSALVAGALSCGYPTYEVQHDVSRVVGGQEASPNSWPW
                     QVSLQYLSSGKWHHTCGGSLVANNWVLTAAHCISNSRTYRVLLGRHSLSTSESGSLAV
                     QVSKLVVHEKWNAQKLSNGNDIALVKLASPVALTSKIQTACLPPAGTILPNNYPCYVT
                     GWGRLQTNGATPDVLQQGRLLVVDYATCSSASWWGSSVKTNMVCAGGDGVTSSCNGDS
                     GGPLNCQASNGQWQVHGIVSFGSTLGCNYPRKPSVFTRVSNYIDWINSVIAKN"
     exon            62..156
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /inference="alignment:Splign:2.1.0"
     exon            157..254
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /inference="alignment:Splign:2.1.0"
     exon            255..383
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /inference="alignment:Splign:2.1.0"
     exon            384..520
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /inference="alignment:Splign:2.1.0"
     exon            521..666
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /inference="alignment:Splign:2.1.0"
     exon            667..819
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /inference="alignment:Splign:2.1.0"
     exon            820..908
                     /gene="Cela2a"
                     /gene_synonym="Ela2; Ela2a; ELAII; Elaii1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acagacatcc acggacacac catgatcagg acactgctgc tatctgcctt ggtggctgga
       61 gctctcagct gcgggtaccc cacttatgag gtccagcatg atgtgagcag ggtagttggg
      121 ggtcaagagg ccagtcccaa cagctggccc tggcaggtct ccctgcagta cctttcctct
      181 ggaaagtggc accacacctg cggaggctcc ctggtggcca acaactgggt tctgaccgct
      241 gcccattgta tcagcaactc taggacctac cgcgtgctgc tgggccgaca cagcctctcc
      301 acctcggaat ccggctcact ggctgttcag gtctccaagc ttgtggtcca tgagaagtgg
      361 aacgctcaaa agctctccaa cgggaatgac attgccctgg tcaaactggc tagcccagtg
      421 gccctgacca gcaagatcca gacggcttgc ctcccacccg ctggtaccat tctgccaaac
      481 aactacccct gctacgtcac aggctggggc cggctgcaga ccaacggagc cactcctgac
      541 gtcctgcagc agggccgtct gctggttgtg gactatgcca cctgctccag cgctagctgg
      601 tggggaagct ccgtgaagac caacatggtg tgcgctggtg gcgacggtgt gacctccagc
      661 tgcaatgggg actccggcgg accactgaat tgccaggcat ctaatggcca gtggcaggtg
      721 cacggtattg tgagctttgg ctccactctg ggctgcaact acccccgtaa gccatctgtc
      781 ttcaccaggg tctccaacta catcgactgg atcaactcgg tgattgcaaa gaactaagca
      841 aaggcatctc tgtgactgtc accttgggaa cgcacggaag ggacacagta ataaagtgac
      901 ttacgaat
//
