LOCUS       NM_016997               2062 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus carboxyl ester lipase (Cel), mRNA.
ACCESSION   NM_016997
VERSION     NM_016997.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Li,X., Lindquist,S., Lowe,M., Noppa,L. and Hernell,O.
  TITLE     Bile salt-stimulated lipase and pancreatic lipase-related protein 2
            are the dominating lipases in neonatal fat digestion in mice and
            rats
  JOURNAL   Pediatr Res 62 (5), 537-541 (2007)
   PUBMED   17805199
  REMARK    GeneRIF: Bile salt-stimulated lipase (BSSL) and PLRP2 is
            responsible for fat digestion as long as milk is the main food.
            Milk-borne BSSL is able to compensate for a slower ontogeny of
            pancreatic BSSL expression in the mouse.
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Raeder,H., Haldorsen,I.S., Ersland,L., Gruner,R., Taxt,T.,
            Sovik,O., Molven,A. and Njolstad,P.R.
  TITLE     Pancreatic lipomatosis is a structural marker in nondiabetic
            children with mutations in carboxyl-ester lipase
  JOURNAL   Diabetes 56 (2), 444-449 (2007)
   PUBMED   17259390
REFERENCE   3  (bases 1 to 2062)
  AUTHORS   Palmer,D.J., Kelly,V.C., Smit,A.M., Kuy,S., Knight,C.G. and
            Cooper,G.J.
  TITLE     Human colostrum: identification of minor proteins in the aqueous
            phase by proteomics
  JOURNAL   Proteomics 6 (7), 2208-2216 (2006)
   PUBMED   16502470
REFERENCE   4  (bases 1 to 2062)
  AUTHORS   Raeder,H., Johansson,S., Holm,P.I., Haldorsen,I.S., Mas,E.,
            Sbarra,V., Nermoen,I., Eide,S.A., Grevle,L., Bjorkhaug,L.,
            Sagen,J.V., Aksnes,L., Sovik,O., Lombardo,D., Molven,A. and
            Njolstad,P.R.
  TITLE     Mutations in the CEL VNTR cause a syndrome of diabetes and
            pancreatic exocrine dysfunction
  JOURNAL   Nat Genet 38 (1), 54-62 (2006)
   PUBMED   16369531
REFERENCE   5  (bases 1 to 2062)
  AUTHORS   Fontaine,R.N., Carter,C.P. and Hui,D.Y.
  TITLE     Structure of the rat pancreatic cholesterol esterase gene
  JOURNAL   Biochemistry 30 (28), 7008-7014 (1991)
   PUBMED   2069957
REFERENCE   6  (bases 1 to 2062)
  AUTHORS   Lindstrom,M.B., Persson,J., Thurn,L. and Borgstrom,B.
  TITLE     Effect of pancreatic phospholipase A2 and gastric lipase on the
            action of pancreatic carboxyl ester lipase against lipid substrates
            in vitro
  JOURNAL   Biochim Biophys Acta 1084 (2), 194-197 (1991)
   PUBMED   1854805
REFERENCE   7  (bases 1 to 2062)
  AUTHORS   DiPersio,L.P., Fontaine,R.N. and Hui,D.Y.
  TITLE     Site-specific mutagenesis of an essential histidine residue in
            pancreatic cholesterol esterase
  JOURNAL   J Biol Chem 266 (7), 4033-4036 (1991)
   PUBMED   1999399
REFERENCE   8  (bases 1 to 2062)
  AUTHORS   DiPersio,L.P., Fontaine,R.N. and Hui,D.Y.
  TITLE     Identification of the active site serine in pancreatic cholesterol
            esterase by chemical modification and site-specific mutagenesis
  JOURNAL   J Biol Chem 265 (28), 16801-16806 (1990)
   PUBMED   2211595
REFERENCE   9  (bases 1 to 2062)
  AUTHORS   Kissel,J.A., Fontaine,R.N., Turck,C.W., Brockman,H.L. and Hui,D.Y.
  TITLE     Molecular cloning and expression of cDNA for rat pancreatic
            cholesterol esterase
  JOURNAL   Biochim Biophys Acta 1006 (2), 227-236 (1989)
   PUBMED   2688744
REFERENCE   10 (bases 1 to 2062)
  AUTHORS   Camulli,E.D., Linke,M.J., Brockman,H.L. and Hui,D.Y.
  TITLE     Identity of a cytosolic neutral cholesterol esterase in rat liver
            with the bile salt stimulated cholesterol esterase in pancreas
  JOURNAL   Biochim Biophys Acta 1005 (2), 177-182 (1989)
   PUBMED   2775770
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Jul 31, 2010 this sequence version replaced NM_016997.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M15893.1, X16054.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                JAXUCZ010000003.1  32288933-32289019   c
            88-238              JAXUCZ010000003.1  32286744-32286894   c
            239-361             JAXUCZ010000003.1  32286542-32286664   c
            362-559             JAXUCZ010000003.1  32286105-32286302   c
            560-690             JAXUCZ010000003.1  32285407-32285537   c
            691-798             JAXUCZ010000003.1  32285148-32285255   c
            799-916             JAXUCZ010000003.1  32284877-32284994   c
            917-1103            JAXUCZ010000003.1  32283518-32283704   c
            1104-1307           JAXUCZ010000003.1  32283188-32283391   c
            1308-1505           JAXUCZ010000003.1  32282413-32282610   c
            1506-2062           JAXUCZ010000003.1  32281518-32282074   c
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p12"
     gene            1..2062
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /note="carboxyl ester lipase"
                     /db_xref="GeneID:24254"
                     /db_xref="RGD:2331"
     exon            1..87
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..1860
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /EC_number="3.1.1.13"
                     /EC_number="3.1.1.3"
                     /EC_number="3.1.1.6"
                     /note="bile salt-activated lipase; sterol esterase;
                     cholesterol esterase; bile salt-stimulated lipase;
                     pancreatic lysophospholipase"
                     /codon_start=1
                     /product="bile salt-activated lipase precursor"
                     /protein_id="NP_058693.2"
                     /db_xref="GeneID:24254"
                     /db_xref="RGD:2331"
                     /translation="MGRLEVLFLGLTCCLAAACAAKLGAVYTEGGFVEGVNKKLSLLG
                     GDSVDIFKGIPFATAKTLENPQRHPGWQGTLKATDFKKRCLQATITQDDTYGQEDCLY
                     LNIWVPQGRKQVSHDLPVMVWIYGGAFLMGSGQGANFLKNYLYDGEEIATRGNVIVVT
                     FNYRVGPLGFLSTGDANLPGNFGLRDQHMAIAWVKRNIAAFGGDPDNITIFGESAGAA
                     SVSLQTLSPYNKGLIRRAISQSGVALSPWAIQENPLFWAKTIAKKVGCPTEDTAKMAG
                     CLKITDPRALTLAYRLPLKSQEYPIVHYLAFIPVVDGDFIPDDPINLYDNAADIDYLA
                     GINDMDGHLFATVDVPAIDKAKQDVTEEDFYRLVSGHTVAKGLKGTQATFDIYTESWA
                     QDPSQENMKKTVVAFETDILFLIPTEMALAQHRAHAKSAKTYSYLFSHPSRMPIYPKW
                     MGADHADDLQYVFGKPFATPLGYRAQDRTVSKAMIAYWTNFAKSGDPNMGNSPVPTHW
                     YPYTTENGNYLDINKKITSTSMKEHLREKFLKFWAVTFEMLPTVVGDHTPPEDDSEAA
                     PVPPTDDSQVVPVPPTDDSQTTPVPPTDNSQAGDSVEAQMPVAIGF"
     exon            88..238
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            239..361
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            362..559
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            560..690
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            691..798
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            799..916
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1103
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1307
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1505
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..2062
                     /gene="Cel"
                     /gene_synonym="Bal; Bssl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcctagaggc agacactcac tatggggcgc ctggaggttc tgtttcttgg cctcacctgc
       61 tgcttggcag ctgcttgtgc tgcaaagttg ggtgctgtgt acacagaagg cggttttgtg
      121 gagggcgtca acaagaaact cagtctcttg ggtggtgact ctgttgacat cttcaagggc
      181 atcccctttg ctaccgccaa gaccctggag aatcctcagc gtcaccctgg ctggcaaggg
      241 acactgaagg ctacagactt caagaaacga tgtctacaag ccaccatcac ccaggatgat
      301 acctatgggc aagaagactg cctctatctc aacatctggg tccctcaggg caggaagcaa
      361 gtgtctcatg acctgcctgt gatggtctgg atctatggag gtgccttcct catggggtct
      421 ggccagggag ccaattttct caagaattac ctgtatgatg gggaagagat cgccactaga
      481 ggcaatgtca ttgtggtcac cttcaactac cgtgtcggac ccttgggttt ccttagcacc
      541 ggagatgcta accttccagg taactttgga cttcgagatc agcacatggc tattgcctgg
      601 gtgaagagga acattgcagc ctttggagga gaccccgata acatcaccat ctttggggaa
      661 tctgctggag ctgccagtgt ctctctgcag accctctccc catacaacaa gggcctcatc
      721 cggcgagcca tcagtcagag tggtgtggca ctgagcccct gggccatcca ggagaatcca
      781 cttttctggg ccaaaacgat cgctaagaag gtgggatgcc ccacagagga taccgccaag
      841 atggctgggt gtctgaagat cacagatccc cgagccttga cactggccta taggttgccc
      901 ttgaaaagcc aggagtaccc cattgtgcac tacctggcct tcatccctgt cgtcgatggt
      961 gacttcattc ctgatgatcc catcaacctg tacgacaacg ctgctgacat tgactactta
     1021 gcgggtatta atgacatgga tggccacctg tttgctacag ttgacgtgcc cgccatcgac
     1081 aaggccaagc aggatgtcac agaggaggac ttctacaggc tagtcagtgg acacactgtc
     1141 gccaaggggc ttaaaggcac ccaagccacc tttgacatct acactgagtc ctgggcccag
     1201 gacccgtccc aggagaacat gaagaagaca gtggtggcct ttgagactga catactcttc
     1261 ctgatcccca cagagatggc tctggcccag cacagagccc atgccaagag tgccaagacc
     1321 tactcttacc tgttttccca cccttcacga atgcctatct acccaaaatg gatgggggca
     1381 gaccacgctg atgacctcca gtacgtcttt gggaagccct ttgccacccc actgggctac
     1441 cgggcccaag acaggactgt ctccaaggcc atgattgcct actggaccaa ctttgccaag
     1501 agtggggacc ccaacatggg caactcaccc gtgcccacac actggtaccc ttataccacg
     1561 gagaatggta actacctgga catcaataag aaaataacca gcacctccat gaaggagcac
     1621 ctaagggaaa agttcctcaa gttctgggct gtgacattcg agatgctgcc cactgtggtt
     1681 ggtgaccaca ctccccctga ggatgactca gaggctgccc ccgtcccacc tacagacgac
     1741 tcccaggttg ttcctgtccc acctacagat gactctcaga caacaccggt gcccccaaca
     1801 gacaactctc aggctggtga ctctgtggag gctcagatgc ctgtcgccat tggcttctaa
     1861 agtcctataa accggggcta gagatggctc aggagctaag agctcttctt ccactgttct
     1921 tctgaaggtc ctgtgttcaa ttcccagcac ccacattgct gcttacagct gtctgattcc
     1981 gtctactggt gtgcagatgt acacgcagac aaagcgccta tatatagaaa attcatacat
     2041 aaataaagtc ttataagcct tg
//
