LOCUS       NM_012524               2673 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus CCAAT/enhancer binding protein alpha (Cebpa),
            transcript variant 1, mRNA.
ACCESSION   NM_012524
VERSION     NM_012524.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Wu,K., Huang,S., Zheng,F. and Liu,Y.
  TITLE     The CEBPA-FGF21 regulatory network may participate in the
            T2DM-induced skeletal muscle atrophy by regulating the
            autophagy-lysosomal pathway
  JOURNAL   Acta Diabetol 60 (11), 1491-1503 (2023)
   PUBMED   37392202
  REMARK    GeneRIF: The CEBPA-FGF21 regulatory network may participate in the
            T2DM-induced skeletal muscle atrophy by regulating the
            autophagy-lysosomal pathway.
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Xu,Z., Yao,X., Zhao,Y. and Yao,B.
  TITLE     C/EBPalpha involvement in microglial polarization via HDAC1/STAT3
            pathway aggravated sevoflurane-induced cognitive impairment in aged
            rats
  JOURNAL   PeerJ 11, e15466 (2023)
   PUBMED   37361037
  REMARK    GeneRIF: C/EBPalpha involvement in microglial polarization via
            HDAC1/STAT3 pathway aggravated sevoflurane-induced cognitive
            impairment in aged rats.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2673)
  AUTHORS   Lu,H., Chen,Y., Chen,Y., Huang,L. and Chen,L.
  TITLE     C/EBPalpha-Mediated Transcriptional Activation of PIK3C2A Regulates
            Autophagy, Matrix Metalloproteinase Expression, and Phenotypic of
            Vascular Smooth Muscle Cells in Aortic Dissection
  JOURNAL   J Immunol Res 2022, 7465353 (2022)
   PUBMED   36132983
  REMARK    GeneRIF: C/EBPalpha-Mediated Transcriptional Activation of PIK3C2A
            Regulates Autophagy, Matrix Metalloproteinase Expression, and
            Phenotypic of Vascular Smooth Muscle Cells in Aortic Dissection.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2673)
  AUTHORS   Duan,P., Wang,H., Yi,X., Zhang,H., Chen,H. and Pan,Z.
  TITLE     C/EBPalpha regulates the fate of bone marrow mesenchymal stem cells
            and steroid-induced avascular necrosis of the femoral head by
            targeting the PPARgamma signalling pathway
  JOURNAL   Stem Cell Res Ther 13 (1), 342 (2022)
   PUBMED   35883192
  REMARK    GeneRIF: C/EBPalpha regulates the fate of bone marrow mesenchymal
            stem cells and steroid-induced avascular necrosis of the femoral
            head by targeting the PPARgamma signalling pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2673)
  AUTHORS   Muller,C., Bremer,A., Schreiber,S., Eichwald,S. and Calkhoven,C.F.
  TITLE     Nucleolar retention of a translational C/EBPalpha isoform
            stimulates rDNA transcription and cell size
  JOURNAL   EMBO J 29 (5), 897-909 (2010)
   PUBMED   20075868
REFERENCE   6  (bases 1 to 2673)
  AUTHORS   Calkhoven,C.F., Muller,C. and Leutz,A.
  TITLE     Translational control of C/EBPalpha and C/EBPbeta isoform
            expression
  JOURNAL   Genes Dev 14 (15), 1920-1932 (2000)
   PUBMED   10921906
REFERENCE   7  (bases 1 to 2673)
  AUTHORS   Lincoln,A.J., Monczak,Y., Williams,S.C. and Johnson,P.F.
  TITLE     Inhibition of CCAAT/enhancer-binding protein alpha and beta
            translation by upstream open reading frames
  JOURNAL   J Biol Chem 273 (16), 9552-9560 (1998)
   PUBMED   9545285
REFERENCE   8  (bases 1 to 2673)
  AUTHORS   Stephens,J.M. and Pekala,P.H.
  TITLE     Transcriptional repression of the C/EBP-alpha and GLUT4 genes in
            3T3-L1 adipocytes by tumor necrosis factor-alpha. Regulations is
            coordinate and independent of protein synthesis
  JOURNAL   J Biol Chem 267 (19), 13580-13584 (1992)
   PUBMED   1618860
REFERENCE   9  (bases 1 to 2673)
  AUTHORS   Thomassin,H., Hamel,D., Bernier,D., Guertin,M. and Belanger,L.
  TITLE     Molecular cloning of two C/EBP-related proteins that bind to the
            promoter and the enhancer of the alpha 1-fetoprotein gene. Further
            analysis of C/EBP beta and C/EBP gamma
  JOURNAL   Nucleic Acids Res 20 (12), 3091-3098 (1992)
   PUBMED   1377818
REFERENCE   10 (bases 1 to 2673)
  AUTHORS   Samuelsson,L., Stromberg,K., Vikman,K., Bjursell,G. and Enerback,S.
  TITLE     The CCAAT/enhancer binding protein and its role in adipocyte
            differentiation: evidence for direct involvement in terminal
            adipocyte development
  JOURNAL   EMBO J 10 (12), 3787-3793 (1991)
   PUBMED   1935900
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000001.1.
            
            On Dec 18, 2013 this sequence version replaced NM_012524.2.
            
            Summary: This intronless gene encodes a transcription factor that
            contains a basic leucine zipper (bZIP) domain and recognizes the
            CCAAT motif in the promoters of target genes. The encoded protein
            functions in homodimers and also heterodimers with
            CCAAT/enhancer-binding proteins beta and gamma. Activity of this
            protein can modulate the expression of genes involved in cell cycle
            regulation as well as in body weight homeostasis. The use of
            alternative in-frame non-AUG (CUG) and AUG start codons results in
            protein isoforms with different lengths. Differential translation
            initiation is mediated by an out-of-frame, upstream open reading
            frame which is located between the CUG and the first AUG start
            codons. [provided by RefSeq, Aug 2014].
            
            Transcript Variant: This variant (1) can initiate translation from
            an upstream non-AUG (CUG) site, and also from three conserved
            downstream, in-frame AUG sites. The isoform (a) represented in this
            RefSeq results from translation initiation at the first AUG start
            codon. Isoform a has a shorter N-terminus, compared to isoform c.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: SRR22582416.154041.1,
                                        SRR26643429.1696.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            regulatory uORF        :: PMID: 10921906
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2673              JAXUCZ010000001.1  96896584-96899256
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..2673
                     /gene="Cebpa"
                     /gene_synonym="DBPCEP"
                     /note="CCAAT/enhancer binding protein alpha"
                     /db_xref="GeneID:24252"
                     /db_xref="RGD:2326"
     exon            1..2673
                     /gene="Cebpa"
                     /gene_synonym="DBPCEP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      97..114
                     /regulatory_class="uORF"
                     /gene="Cebpa"
                     /gene_synonym="DBPCEP"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:10921906]"
                     /note="this uORF is predicted to encode a 5 aa peptide
                     (PMID:10921906)"
     CDS             122..1198
                     /gene="Cebpa"
                     /gene_synonym="DBPCEP"
                     /note="isoform a is encoded by transcript variant 1; C/EBP
                     alpha; CAAT/enhancer-binding protein, DNA-binding protein;
                     CCAAT/enhancer binding protein (C/EBP), alpha"
                     /codon_start=1
                     /product="CCAAT/enhancer-binding protein alpha isoform a"
                     /protein_id="NP_036656.1"
                     /db_xref="GeneID:24252"
                     /db_xref="RGD:2326"
                     /translation="MESADFYEAEPRPPMSSHLQSPPHAPSNAAFGFPRGAGPAPPPA
                     PPAAPEPLGGICEHETSIDISAYIDPAAFNDEFLADLFQHSRQQEKAKAAAGPAGGGG
                     DFDYPGAPAGPGGAVMSAGAHGPPPGYGCAAAGYLDGRLEPLYERVGAPALRPLVIKQ
                     EPREEDEAKQLALAGLFPYQPPPPPPPPHPHASPAHLAAPHLQFQIAHCGQTTMHLQP
                     GHPTPPPTPVPSPHPAPAMGAAGLPGPGGSLKGLAGPHPDLRTGGGGGGGAGAGKAKK
                     SVDKNSNEYRVRRERNNIAVRKSRDKAKQRNVETQQKVLELTSDNDRLRKRVEQLSRE
                     LDTLRGIFRQLPESSLVKAMGNCA"
ORIGIN      
        1 cgacccaaag ctgcgcgggc gcgggcgagt tggggtactg ggtgggcggc ggcgacagcg
       61 gcgccacgcg caggctggag gccgccgagg ctcgccatgc cgggagaact ctaactcccc
      121 catggagtcg gccgacttct acgaggcgga gccgcggccc ccgatgagca gccacctcca
      181 gagccccccg cacgcgccca gcaacgccgc ctttggcttt ccccggggcg cgggccccgc
      241 gccgccccca gccccacctg ccgccccgga gccgctgggc ggcatctgcg agcacgagac
      301 gtctatagac atcagcgcct acatcgaccc ggccgccttc aacgacgagt tcctggccga
      361 cctcttccag cacagccggc agcaggagaa ggccaaggcg gcggcgggcc ccgcgggtgg
      421 cggcggtgac tttgactacc cgggcgcccc ggcgggcccc ggcggtgcgg tcatgtccgc
      481 gggggcgcac ggaccccctc ccggctacgg ctgtgcggcg gccggctacc tggacggcag
      541 gctggagccc ctgtacgagc gcgtcggggc gcccgcgctg cggccgctgg tgatcaagca
      601 ggagccccgc gaggaggacg aggcgaagca gctggcgctg gccggcctct tcccctatca
      661 gcccccgccg ccgccgccgc caccgcaccc gcacgcgtct cccgcgcact tggccgcccc
      721 tcacttgcag ttccagatcg cacactgcgg ccagaccacc atgcacctgc agcctggcca
      781 ccctacgccg ccgccgacgc ccgtgcccag ccctcatccc gcgcctgcaa tgggtgctgc
      841 gggcctgccg ggccccgggg gctcgctcaa gggcttggct ggtccgcacc ccgacctccg
      901 caccggcggc ggcggcggcg gcggggccgg cgcgggcaag gccaagaagt cggtggataa
      961 gaacagcaac gagtaccggg tacggcggga acgcaacaac atcgcggtgc gcaagagccg
     1021 agataaagcc aaacagcgca acgtggagac gcagcagaag gtgttggagt tgaccagtga
     1081 caatgaccgc ctgcgcaagc gggtggaaca gctgagccgt gaactggaca cgctgcgggg
     1141 tatcttccgc cagctgcctg agagctcctt ggtcaaggcc atgggcaact gcgcgtgagg
     1201 cgcgcggctg cgggaccgcc ttgagccggc cccctggctg gggacccgga ggatggttta
     1261 gggtcgctgg atctctaggc tgcccggacc gtgcaagcca ggactaggag attctggtgt
     1321 cgcctgaaag cctggcctgc tccgcgtgtc ccctcccttc ctctgagccg gactcggtgc
     1381 gtctaagatg agagggagtc aggccgtggt ggtttctcct ttagaccgag agacttctcc
     1441 gcggagctga gctgggagcc cggcagtttt agtattaagg aagtaacctt gtgccttgga
     1501 tactcaaaac tcgctccttt tcctaccgag tagggggagc aaacatgtgc cttgatattt
     1561 tatttggagg attcctgctt cctccagagc tgcagcaggc ccccatgaga aaaatgaagg
     1621 gtgcaggccc agggcaggag gaagattcag gaagctgagg tcccagcagt ccctgagctg
     1681 cccctccgtc cctgtcctta gaggggaggg acttagggag ttggggattt gagtcttaaa
     1741 agtgtcccca cccctagtta catggagatg gagggttctt agccccttgc ttttcgctcc
     1801 tccatctaca ttcctccccc cacccccagc ttacaacagg ccaggtttcc ttggtgagtt
     1861 cgtggagaag ggggcaacac ccccagtcag accagaaggc tgagttgtga actggccatg
     1921 tggttaagca agtggtagaa gacagggacc ttagtttctg gtcttggggt ggaggatgaa
     1981 gacactggga ccatccgcct tgtgtgtact gtatgtcgcc agccgctgtt gctgaaggaa
     2041 cttgaagcat aatcgatcca tcccagaggg actggagtta tgacaagctt cccaaatatt
     2101 ttgctttatc atctgatatc aacacttgta tctggtctct gtgtcccagc ggtgccttgt
     2161 gcaatgtcag tgtgcgcgtc tatgctaaac caccatttta tttggtcttt tgttttgttt
     2221 tggttttgct ctgattcttg ccaaaatgag actcttcacg aacggccagg ggaaggggct
     2281 gagtgaggct ctcattcttt ttggtttagg gatgtttggt ttttttgttt tgttttgttt
     2341 tgttttgttt tctttcgcct cccagaggac caatgaaatg aagtgggctt ctccctctcc
     2401 cctagttttc caagggtgta tgtagttgtg ggtcttagct tccttcctcc ggctgagact
     2461 tatactggct tccccaccca cccaccccca tccccaaagg ccctggctct gggtctggaa
     2521 agaaggccac ctccagccaa tttgtacaca accctgccac tggaagcagg ggtggactgc
     2581 ttccttctct tctttttttg gggggagggg acacaaagtt tcatgctaga tgtcctatgt
     2641 attatatcta taatataaac atatcaaact caa
//
