LOCUS       NM_001421533           10047 bp    mRNA    linear   ROD 01-JUL-2024
DEFINITION  Rattus norvegicus cyclin-dependent kinase-like 5 (Cdkl5), mRNA.
ACCESSION   NM_001421533 XM_017602291
VERSION     NM_001421533.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Simoes de Oliveira,L., O'Leary,H.E., Nawaz,S., Loureiro,R.,
            Davenport,E.C., Baxter,P., Louros,S.R., Dando,O., Perkins,E.,
            Peltier,J., Trost,M., Osterweil,E.K., Hardingham,G.E., Cousin,M.A.,
            Chattarji,S., Booker,S.A., Benke,T.A., Wyllie,D.J.A. and Kind,P.C.
  TITLE     Enhanced hippocampal LTP but normal NMDA receptor and AMPA receptor
            function in a rat model of CDKL5 deficiency disorder
  JOURNAL   Mol Autism 15 (1), 28 (2024)
   PUBMED   38877552
  REMARK    GeneRIF: Enhanced hippocampal LTP but normal NMDA receptor and AMPA
            receptor function in a rat model of CDKL5 deficiency disorder.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Kontaxi,C., Ivanova,D., Davenport,E.C., Kind,P.C. and Cousin,M.A.
  TITLE     Epilepsy-Related CDKL5 Deficiency Slows Synaptic Vesicle
            Endocytosis in Central Nerve Terminals
  JOURNAL   J Neurosci 43 (11), 2002-2020 (2023)
   PUBMED   36759195
  REMARK    GeneRIF: Epilepsy-Related CDKL5 Deficiency Slows Synaptic Vesicle
            Endocytosis in Central Nerve Terminals.
REFERENCE   3  (bases 1 to 10047)
  AUTHORS   Schroeder,E., Yuan,L., Seong,E., Ligon,C., DeKorver,N.,
            Gurumurthy,C.B. and Arikkath,J.
  TITLE     Neuron-Type Specific Loss of CDKL5 Leads to Alterations in mTOR
            Signaling and Synaptic Markers
  JOURNAL   Mol Neurobiol 56 (6), 4151-4162 (2019)
   PUBMED   30288694
  REMARK    GeneRIF: the molecular pathology in CDKL5 disorder may have
            distinct neuron-type specific origins and effects.
REFERENCE   4  (bases 1 to 10047)
  AUTHORS   Canning,P., Park,K., Goncalves,J., Li,C., Howard,C.J., Sharpe,T.D.,
            Holt,L.J., Pelletier,L., Bullock,A.N. and Leroux,M.R.
  TITLE     CDKL Family Kinases Have Evolved Distinct Structural Features and
            Ciliary Function
  JOURNAL   Cell Rep 22 (4), 885-894 (2018)
   PUBMED   29420175
REFERENCE   5  (bases 1 to 10047)
  AUTHORS   Hector,R.D., Dando,O., Ritakari,T.E., Kind,P.C., Bailey,M.E. and
            Cobb,S.R.
  TITLE     Characterisation of Cdkl5 transcript isoforms in rat
  JOURNAL   Gene 603, 21-26 (2017)
   PUBMED   27940108
  REMARK    GeneRIF: Characterization of Cdkl5 transcript isoforms in the rat
            has been described.
REFERENCE   6  (bases 1 to 10047)
  AUTHORS   Moseley,B.D., Dhamija,R., Wirrell,E.C. and Nickels,K.C.
  TITLE     Historic, clinical, and prognostic features of epileptic
            encephalopathies caused by CDKL5 mutations
  JOURNAL   Pediatr Neurol 46 (2), 101-105 (2012)
   PUBMED   22264704
REFERENCE   7  (bases 1 to 10047)
  AUTHORS   Chen,Q., Zhu,Y.C., Yu,J., Miao,S., Zheng,J., Xu,L., Zhou,Y., Li,D.,
            Zhang,C., Tao,J. and Xiong,Z.Q.
  TITLE     CDKL5, a protein associated with rett syndrome, regulates neuronal
            morphogenesis via Rac1 signaling
  JOURNAL   J Neurosci 30 (38), 12777-12786 (2010)
   PUBMED   20861382
  REMARK    GeneRIF: these results demonstrate a critical role of CDKL5 in
            neuronal morphogenesis and identify a Rho GTPase signaling pathway
            which may contribute to CDKL5-related disorders.
REFERENCE   8  (bases 1 to 10047)
  AUTHORS   Carouge,D., Host,L., Aunis,D., Zwiller,J. and Anglard,P.
  TITLE     CDKL5 is a brain MeCP2 target gene regulated by DNA methylation
  JOURNAL   Neurobiol Dis 38 (3), 414-424 (2010)
   PUBMED   20211261
  REMARK    GeneRIF: Cdkl5 is a MeCP2-repressed target gene providing a link
            between genes the mutation of which generates overlapping symptoms:
            DNA methylation changes are a potential mechanism participating in
            the long-term plasticity triggered by pharmacological agents
REFERENCE   9  (bases 1 to 10047)
  AUTHORS   Bertani,I., Rusconi,L., Bolognese,F., Forlani,G., Conca,B., De
            Monte,L., Badaracco,G., Landsberger,N. and Kilstrup-Nielsen,C.
  TITLE     Functional consequences of mutations in CDKL5, an X-linked gene
            involved in infantile spasms and mental retardation
  JOURNAL   J Biol Chem 281 (42), 32048-32056 (2006)
   PUBMED   16935860
REFERENCE   10 (bases 1 to 10047)
  AUTHORS   Mari,F., Azimonti,S., Bertani,I., Bolognese,F., Colombo,E.,
            Caselli,R., Scala,E., Longo,I., Grosso,S., Pescucci,C., Ariani,F.,
            Hayek,G., Balestri,P., Bergo,A., Badaracco,G., Zappella,M.,
            Broccoli,V., Renieri,A., Kilstrup-Nielsen,C. and Landsberger,N.
  TITLE     CDKL5 belongs to the same molecular pathway of MeCP2 and it is
            responsible for the early-onset seizure variant of Rett syndrome
  JOURNAL   Hum Mol Genet 14 (14), 1935-1946 (2005)
   PUBMED   15917271
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On May 20, 2023 this sequence version replaced XM_017602291.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643299.67889.1,
                                           SRR26643298.31803.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-89                JAXUCZ010000021.1  37566378-37566466
            90-320              JAXUCZ010000021.1  37629828-37630058
            321-355             JAXUCZ010000021.1  37633355-37633389
            356-401             JAXUCZ010000021.1  37688722-37688767
            402-538             JAXUCZ010000021.1  37698857-37698993
            539-659             JAXUCZ010000021.1  37703739-37703859
            660-719             JAXUCZ010000021.1  37705906-37705965
            720-810             JAXUCZ010000021.1  37708527-37708617
            811-1000            JAXUCZ010000021.1  37712595-37712784
            1001-1081           JAXUCZ010000021.1  37721046-37721126
            1082-1233           JAXUCZ010000021.1  37726867-37727018
            1234-2200           JAXUCZ010000021.1  37732543-37733509
            2201-2302           JAXUCZ010000021.1  37737300-37737401
            2303-2408           JAXUCZ010000021.1  37737673-37737778
            2409-2532           JAXUCZ010000021.1  37743049-37743172
            2533-2632           JAXUCZ010000021.1  37749960-37750059
            2633-2752           JAXUCZ010000021.1  37755268-37755387
            2753-10047          JAXUCZ010000021.1  37758444-37765738
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq14"
     gene            1..10047
                     /gene="Cdkl5"
                     /note="cyclin-dependent kinase-like 5"
                     /db_xref="GeneID:100362725"
                     /db_xref="RGD:2324133"
     exon            1..89
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            90..320
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..3139
                     /gene="Cdkl5"
                     /EC_number="2.7.11.22"
                     /codon_start=1
                     /product="cyclin-dependent kinase-like 5"
                     /protein_id="NP_001408462.1"
                     /db_xref="GeneID:100362725"
                     /db_xref="RGD:2324133"
                     /translation="MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKF
                     KDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEE
                     MPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNL
                     SEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEID
                     QLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKN
                     LLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSTKRKPYHVESSTLSNRSQSTKGGA
                     LQTHHRSNSKDIQNLSVGLPRAEEGLPANESFLNGNLAGATLSPMHTKTYQASTQPGS
                     SSKDLTNNNIPHLLSPKEAKSKTEFDFNIDPKPSEGPGTKYLKSSTRSQQNRHSFMES
                     SQSKAGTLQPSEKQSRHSYIDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQI
                     TETNTSRYFPSSCLDLNSPTSPTPTRHTDTRTLLSPSGRNNRNEGTLDSRRTTTRHSK
                     TMEELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDKVRAKG
                     IDGSLSIGQGMAARANSLQLLSPQPGEQLPPEMTVARPSVKESSREGTSSFHTRQKSE
                     GGVYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTRVSSLP
                     SDSSSGTNHSKRQPGFDPWKSPENISHSDQLKEKEKQGFFRSMKKKKKKTQTVPNTDG
                     PDLLTLQKAIHSSSTASSRPKEWRPEKLSDLQTQSQPLKSLRKLLHLSSSTNHPASSD
                     PRFQPLTAQQAKNSFSEIRIHPLSQASGGSSNIRQEPTPKGRPALQLPGQMDPGWHVS
                     SVTRSATEGPSYSEQLGAKSGPNGHPYNRTNRSRMPNLNDLKETAL"
     exon            321..355
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            356..401
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            402..538
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            539..659
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            660..719
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            720..810
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            811..1000
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1081
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1233
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..2200
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2302
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2408
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2532
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2632
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..2752
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
     exon            2753..10047
                     /gene="Cdkl5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acttgtcgct accgctaggg agaaggtaaa gcggtgacgg cggccgccgg agtggaggct
       61 tccccggtag aggtggggga ctcagtaggg gactcattta atacttcaca gttgaacaaa
      121 tgtatgaacg ttccttgcca accattgaga atttcttcgt tgggacattt ttttcctgca
      181 tactgaataa ctggtttaga agtccctttt ttggtgccat gttctgtggc ttgaatcaaa
      241 agaggagttt gtcttcatga agattcctaa cattggtaat gtgatgaata aatttgagat
      301 ccttggggtt gttggtgaag gagcctatgg agttgtactt aaatgcagac acaaggaaac
      361 acatgaaatt gtggcaatca agaaattcaa ggacagtgaa gaaaatgaag aagtcaagga
      421 gacgacttta cgagagctta aaatgcttcg cactctcaag caggaaaaca ttgtggagct
      481 gaaggaagct tttcgtcgga gaggaaaact atacctggtg tttgaatatg ttgagaaaaa
      541 tatgcttgaa ttgctggaag agatgccaaa cggagttcca cctgaaaagg ttaaaagcta
      601 tatctaccaa ctaatcaaag ccatccactg gtgccacaag aacgacattg tccacagaga
      661 tataaagcct gaaaatctct taatcagcca caatgatgtc ctgaaactgt gtgactttgg
      721 ttttgctcgg aatctctcag aaggaaacaa tgctaattac acagaatacg tggctactcg
      781 gtggtaccga tctccagaac tgctacttgg agccccctat ggaaagtcag tggacatgtg
      841 gtcagtgggc tgtattcttg gagagctcag tgatggacag cccttatttc ctggggaaag
      901 tgaaattgac caactcttta ctattcagaa ggtgctagga ccacttccgt ctgagcagat
      961 gaagctcttc tacagtaatc ctcgtttcca tgggctccgg tttccagctg ttaaccatcc
     1021 tcagtcatta gagagacgat accttggaat tttaaatagt gttttacttg acctaatgaa
     1081 gaatttactg aagttggacc cagctgacag atacttgacg gagcagtgtt tgaatcaccc
     1141 tacatttcaa actcagagac ttttggaccg gtctccttca aggtcaacaa aaaggaagcc
     1201 ttaccatgtg gaaagcagca cactgtctaa tagaagccaa tccaccaagg gtggtgcttt
     1261 acagactcat cacagatcca acagcaagga cattcagaac ttaagtgtgg gtctgcccag
     1321 agctgaggaa ggcctgcctg caaatgaaag cttcctaaat ggaaaccttg ctggagcaac
     1381 tctcagtcca atgcacacca aaacgtacca agcaagtacc cagcctgggt cttccagcaa
     1441 agatctcaca aacaacaata taccgcacct tctcagtcca aaagaagcca agtccaaaac
     1501 agagtttgac ttcaatattg acccaaagcc ttcagaaggt ccaggcacaa agtacctcaa
     1561 gtccagcacc agatctcaac agaacagaca ctcattcatg gaaagttccc agagcaaagc
     1621 tgggacactg cagcctagtg agaagcagag tcggcacagc tatattgaca ccatccccca
     1681 gtcttccagg agtccatcct acaggacaaa ggccaaaagc catggggctt tgagtgactc
     1741 caagtctgtg agcaaccttt cagaagccag agcccaaatc acagagacta ataccagtag
     1801 atatttccca tccagttgtt tggacttgaa ctctcccacc agcccaacac ccaccaggca
     1861 cactgatacg agaactttgc tcagcccttc cgggagaaat aatcgaaatg agggcactct
     1921 ggactcccgg cgaaccacca ccagacactc aaaaaccatg gaagagttga agctgcctga
     1981 acacatggat agcagccatt cacattcgct gtctgcacct catgaatcat tttcttatgg
     2041 gctaggctat accagcccct tctcctctca acaacgtcct cacaggcatt ccatgtatgt
     2101 gacccgggac aaagtgagag ctaaaggcat tgacggaagc ttgagcatag ggcaagggat
     2161 ggcagccaga gccaacagct tgcagctgtt atcaccccag cctggagaac agcttcctcc
     2221 agaaatgact gtggcaagac cttctgtaaa agagtcctcc cgagaaggca cctcttcgtt
     2281 ccacacccgt cagaaatctg agggtggagt ataccatgac ccacactctg acgacggcac
     2341 ggctcccaaa gaaaatcggc atctgtacaa tgatcctgtc ccaaggagag ttggtagctt
     2401 ttatagagtg ccatctccac gaccagacaa ttctttccat gaaaataatg tgtccactcg
     2461 agtttcttct ctgccatcgg atagtagttc tggcacaaat cattcaaaaa gacaaccagg
     2521 atttgatccg tggaaaagtc ctgaaaacat tagtcattcc gatcaactca aagaaaagga
     2581 gaagcaaggc tttttcaggt caatgaaaaa gaaaaagaag aaaactcaaa cagtgcccaa
     2641 cactgatggt cctgatttat tgaccttaca gaaagccatt cattcttcta gcactgcaag
     2701 cagcagacca aaggagtggc gacctgaaaa gctttcagat ctacagaccc agagccaacc
     2761 actaaaatcc cttcgcaagc tgctgcatct ttcttcgtca accaatcacc cggcttcttc
     2821 agatcctcgg ttccagccgt taacagctca acaagccaaa aattccttct cagaaattcg
     2881 gattcaccct ctgagtcagg cctctggtgg gagcagcaac atccggcagg agcctacacc
     2941 aaaaggcaga ccagcccttc agctgccagg tcagatggat cctggttggc acgtgtcctc
     3001 tgtgaccagg agtgccacag aggggccttc ctattctgaa cagctaggtg ccaagagtgg
     3061 gccaaacggg cacccttata acagaacaaa tcgctcacga atgccaaatc tgaatgattt
     3121 aaaagaaaca gccttgtaat ttatgctggg atggagtgtg tgtggggggt ggggtggggg
     3181 tggggagaca aaccagttgg gaggggaggg tccagggtgg gctgggaaat gggttagacc
     3241 agggtaataa gccagtattt tcagctttat gaaggtgtgt gcaatattgt gtgtgagggg
     3301 ggccatctgg cccctcacaa ccacaaatgc tagtcaggga tcttaagagc cacgggagtt
     3361 ccttagggat caccactccc cacaggtctt gtgtgagaat ggatagagtg tgccattgag
     3421 gaagaagaaa ttcttgccag tttctcccca ttacattcca gctttagtgc aatctctgtt
     3481 gaacttgggg aagccagggt gtgtcctggg tgcaagggat agaaaaattc atgttgaccg
     3541 atgcccttac cacatatttt tttctgacta gactaaatgt ggggtttatt gtaatctagg
     3601 agtatccctt tgaagggtta gcagatgact gtatgtctta aattgttttc taagcttcca
     3661 tatttgatag gatttgtaac atttatttat aattaatatt gtactgttat aatcatacct
     3721 tttatgttat aatgtaaagt tattttgagc tgtttggaac attgtgaagc agtgggacag
     3781 ctacaatagt ttctataaaa actaaacagt aacatttata tattgcatca ggagtatttt
     3841 attctatata taaatatata tatggtaaat aactgtctgt tttataaata tattcattta
     3901 aagaaagtat cgttatgaca ctatctgcca tatttttata catttgtgat atgaagtgag
     3961 tattatactt ataataaagg gagttgaatt gtggctacat gtgattgtaa cagtataaaa
     4021 ctttgctcaa ctttttggcc ccagcagtag cctctagaca tgatccttgg tagcagacaa
     4081 atacagcatc tctttcaagg cccacaggtg aagaaggttg gataagctag taaggagccc
     4141 aacaggcctc atttctgtcc tcagacactt ggctctcatt tgtgtccagg gacattaact
     4201 ggtacacgag agaaggacag acagacgtgg tgtgcatggg tctttaccga gaatgtgctc
     4261 ttccactcag cattagtgtt agagtgtaga ccaaagattg ccagtgaaca ttccttgttg
     4321 tgaggttgta agaggcacct gtctgcggat cttgcaaacc agctctatgc tcctgaatgc
     4381 tgtccgccct cccggagact cttgcctcca ggtgggatct gagctatgtg tatggcattt
     4441 cttcttctaa ctgctttgtt gttgttcttg ttgttgttgc tcttctaata tatttataag
     4501 gctcctgaag caattccata tgtaaagaaa actgctatgt ccttttttcc ttcccgggac
     4561 tgtccattta taacaaggtc atcgtgtaca cctgtatata catacactca ctccatccat
     4621 cagaacagga ctacatattt ctctcatttg atcactctca actcccgagt tactatcaag
     4681 gatcaccaag gcctctggcc agcaagcatt attcccacct gtctatggac catctttgac
     4741 aaaaaaaaaa caaaaacaaa aaaacaaaaa caaaaagaac aacaaaaaca aaaaacaaaa
     4801 aactcaaaca cctcaacacc atcatctatc aatagtcata gagttttgaa aagtagtctc
     4861 ttagcaataa gacttttaga gatcactccc atccctcaga gaggtaactt ttcagtattt
     4921 actacttatt ccagaattct gagagtgtca gtccccaaat aaagtcagct gtcattgaaa
     4981 tatgctgagt aactgttaac ccttccctga gattttgaga atcatagcac tgatgattat
     5041 gaagattata aaggtcttag attctttgca gcttaaattg tgtgcatatt gcatatttat
     5101 ataaatacta ttaatgatgt gtattgatta tatatagagg tcattttaag gtgcttttta
     5161 tttaatttta ataagtataa taggcactaa aatgaaactc aactggatac tatcatggaa
     5221 acaatttgat tcattttgca tgctcttgag ttttttccta tcgtttctgt taccagttca
     5281 gtactgtctg ggtagctctg aattctggct ttccccaggc aactcttcag ttacctaagt
     5341 gaggtagacc ctcagcagtt tttgcctgga tgtagtgaca ctaaaaaggt gtatatttaa
     5401 ctcttctttt tcttactaga tctgtacctt tgtatcccct gccccctgcc ccctgcaaac
     5461 cagaaactac attttgtttc ctctggaaag acttctcact gtgctgtgtg cttaggaact
     5521 gcgaggtccc attgccatgc tgtggcattc gtggctcatc atcaccaagg ggctggtttc
     5581 ccatgttgtc acctttgtta acactgggat ctcagatgtt tgcagaacat ttatattcct
     5641 gatggttctt taaagaaggg ctagaacaat tgccttctac ctagagcaaa ctaatcctaa
     5701 ctaactgatg aagtcaacac ttaacttttc ctacattccc agatttgagc cagaaaatag
     5761 ctacaatgtt ttcaactagt gtttctgagc tagctctttt atcctcttct tagctcttgt
     5821 ctgttctgac ttttccttga tcctcaataa gctataagtg aatctacaat cccatttatt
     5881 ctcttactgc tcccagatgg ggggcagtaa cattttctct cagatctaat gagccctctc
     5941 ttagctttta actgtcactt ccccttctga ttggtcattc attggcctca aaaagatgcc
     6001 atccacggca ggaaggagtt gactggaagt cacaaccccc ccacacacat tttctttagg
     6061 agggggaatt gatggcatcc atgttctgtt atctgtgccg acagactaga ggacacaaag
     6121 gttgaattga aggcttatga agtactattg ctgattacct ttcaaaaaga accttggttt
     6181 gacagtgtct ctaatttttc ttctctagca tcctcttttt ctttcctgaa gttctaagta
     6241 aactttaact gatctttgtt cagtcttaac tgacgtatag gacactctcc agggcccaag
     6301 accagaagag agtgcccaat gccaagaggt tcgagtgagc tatctctctg cagctgcact
     6361 tcttagccag ggccagtgtt gtccaccggg cacatttgac aatgtccgga gacatttggt
     6421 tgtcttgggc tactcccagc atctagtgag tagaggccag ggatgttgct agacatcccc
     6481 cagagtattt ccagccattt ctactaccaa ggttaagaag tcctgcttga gaccaatcca
     6541 ggccaaccac tctgcagaat ttgcctctgg ccttgggctt tccaggctac ataattgcaa
     6601 gagtgagtcc cttaacttat ttaagaatcc tctaccactg ttctcaaatc tttgcaacct
     6661 attgttcgtt tccattgtct tggacctgct aaaaggtgca tactttggga ggaaaccaga
     6721 gttactttat atatgtttga gaacttaaat tctagattag catccttaga agatctttct
     6781 tagccatgag gcttatattt agaagcaagc agcagcctgg cagattggcc tgatttttac
     6841 caactgctca aaggcatcat ggctttcagc tgtgtctacc gaaagaaaat gtctttgttt
     6901 tttttttttt ttattcaagt cgtttaatag ctctttaaaa aatattagca tatggcagac
     6961 tgattagaag caaaactctt tcttggtggt tgtgacgtgg ctattctaga agtattgtgc
     7021 tgtatgcttt ggttaaatct gttttaaaac atgctttaag actcaccata tgcagttgta
     7081 tactttaatt ctaagcagac ctattctggt cagtctctag tcatgccgtg tattataaaa
     7141 gtatgttgtg gttgtagagt tagccagttt agcatgttcc taatcagaaa attagtggac
     7201 tccactagga aatgctatcc cattttccct tcccagtacc tcttagttga tatcatagta
     7261 tcctcactta aactagtctt tagagtaaat aaggaaaaaa aagccattta ttttcttcta
     7321 gccatgtatt gagaataact cctatgtgta taattttttc aaaggccaga ggattacttt
     7381 tcccatatgt agaaggcttt gtccattctg ttcacctagg tctcagtggt caaagaactt
     7441 ttggcaagga tgataagtag ctcattggaa gtcgctgctg gggctcggct gggactcagc
     7501 aaaattgcct atctcttgtg ctcagaacct gtgttctgga tgacactact ttttcgttcg
     7561 gaagttcatt gcctactgca ttagagtggg ctatttaacc ccactatgtg ggtacataca
     7621 aagatccaca gatccctact tctgtgaaca gggactatga tgttaggttt caccaaccca
     7681 aggcagaaag cagtgggata tttatttcct tgcctccttt agttcatcac tggtaaaagt
     7741 tccttgaaac tcacttatgg cttaagaaat aacagttctg gtttctttaa aagacattgc
     7801 agacaagaca ggacacaagt cagtcatccc caggagtaga atcttaggag aaggtcccta
     7861 ccaaattgta aaaggccaga ttaccagaag ataaaacaga caaacaaatc cagacttgta
     7921 attggttgtg gtttcctctt ttgcttcttg accacccaaa ttctacaata acttaagggt
     7981 cttgttcaac tagaaagctc aatcttagaa accgtgagtg aggagccatg caagtaaaga
     8041 aaagccctta tctgtttgca tacacccaaa gggaaaagcc aagtgactag gaaactctgg
     8101 gcctattgcc catgcaattg ttttttaatg cagtgtacta tacaagaagg tagactttgt
     8161 gcaaacccgt ttcttacaaa aatcattgtt gcccacagtg ctttgttcaa ttcatcatca
     8221 tttggccagg tccctactcc acctttttcc tctctgggca tgacccacct ttctttctgt
     8281 ttattgtagg ctgttaaata tgatctgacc tgctttgtac tttcattcaa ctgtttactc
     8341 ccgcacttaa gggagggttg tctcttgttt tttgccagaa aaataaataa ataaataaag
     8401 ttgtcatagt ctgtacgctg gatttgtagg gccagcagaa ctgctggagt gaaactggtt
     8461 tcactggtaa ggcccttctt cctttccaag ggtgagctct ctacaccccc tttgatcctc
     8521 ggtccaactg gtcctccttg ggaatctgaa atcagtctcc gtgttgtatg cagattagaa
     8581 gttgccttgt ttgctgctct tcccaggcag agtatcaggg agaaagaggc cttatctgtc
     8641 cctccgtgtc ccctcctgtt tggcagaccc gctgagaatc cctcaggact tcctttggtt
     8701 ggggatgtcc cttcccagaa ggctgatgtg attcatttca ggcatagaca agcttgtcct
     8761 agtgctgtgc ttcaggtcta atcagaagaa acccaggaat caaaaaggta gatgccttga
     8821 cttttgtccc tgttggggga ttaaagtgtt tattgccaga atcatcaaaa tctctggtta
     8881 aactcagtag agtttcagag aaagactatg cttttccttg tggagaatgt atactctcca
     8941 tactggcttc ctttctccaa aagtggaaag taaagctcgc agaaaatcca tttccctcgg
     9001 gatgatcagt gggttcttag tcactagtct ctctccctct ccttgtttac tctttttggt
     9061 cagtgatgga gttggcctgg gggaggagca gcctatgtgg tgacagaaag aaatagattc
     9121 tcactgtaaa cttgcattga tgaacagaga caattaagcc aaacgaaaca gttaccccag
     9181 agttctagct ccaagaattg ctttgtgcca cactgaattc aggtcgttat tctgtatata
     9241 tttatatcat tttcagagcg tcttcagtct ttactaagtt tgaagggtat ggttttctgg
     9301 aaagacacag ggcagggcga cactgagatc cctgtacctt cttaaagaca aagattgtga
     9361 taagccagag ggatatgcca tctgatgtgc ggtagatgga aatcagtgga cattcttaat
     9421 tgcatagact ttataatcta ctatataaag tgtaatccat actttttact gtggcaaggg
     9481 tgtgatgtgg ttgttaatct ttttaatttt tgaatccaaa ctgaatttaa agtgttgaat
     9541 acttacaatt cctcacaagc taattatgaa ccatttgtaa agtgtttcta taactctttg
     9601 tatcatgtgt tggtacatcg tatcaagttt tttctggcat gtatcccatt ctaaacttct
     9661 gtaaaatttc tattgttgct gtacatattc tacacatatc tactctgtgg taaccttcat
     9721 taacagcatg aagtggttaa tttttactga gcacaatgta tttttgaatg gatcttccca
     9781 catgtcttta ataaaatgca gattttgcac tgatgtgact gccaggcttt ccccattttg
     9841 agcactgtgg tccttcttgc ctttgaacag cgagcagctc cattctaatt cttccttggt
     9901 aaacaagaat atatgttcga atgcaaatga cgggcaatag gcctttgaag ttgtaatcac
     9961 tttaaatgag tgaaattgat gtaaaacaaa gcaaatcctg ttacttaatg attttttttt
    10021 tctatttata aagttctttt cccctta
//
