LOCUS       NM_145721               1975 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus CDK5 regulatory subunit associated protein 1
            (Cdk5rap1), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_145721
VERSION     NM_145721.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,F.Y., Zhou,B., Suzuki,T., Miyata,K., Ujihara,Y., Horiguchi,H.,
            Takahashi,N., Xie,P., Michiue,H., Fujimura,A., Kaitsuka,T.,
            Matsui,H., Koga,Y., Mohri,S., Suzuki,T., Oike,Y. and Tomizawa,K.
  TITLE     Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs
            governs protein translation and contributes to myopathy in mice and
            humans
  JOURNAL   Cell Metab 21 (3), 428-442 (2015)
   PUBMED   25738458
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Ching,Y.P., Pang,A.S., Lam,W.H., Qi,R.Z. and Wang,J.H.
  TITLE     Identification of a neuronal Cdk5 activator-binding protein as Cdk5
            inhibitor
  JOURNAL   J Biol Chem 277 (18), 15237-15240 (2002)
   PUBMED   11882646
REFERENCE   3  (bases 1 to 1975)
  AUTHORS   Wang,X., Ching,Y.P., Lam,W.H., Qi,Z., Zhang,M. and Wang,J.H.
  TITLE     Identification of a common protein association region in the
            neuronal Cdk5 activator
  JOURNAL   J Biol Chem 275 (41), 31763-31769 (2000)
   PUBMED   10915792
REFERENCE   4  (bases 1 to 1975)
  AUTHORS   Ching,Y.P., Qi,Z. and Wang,J.H.
  TITLE     Cloning of three novel neuronal Cdk5 activator binding proteins
  JOURNAL   Gene 242 (1-2), 285-294 (2000)
   PUBMED   10721722
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC066666.1.
            
            On Jun 10, 2007 this sequence version replaced NM_145721.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066666.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q41"
     gene            1..1975
                     /gene="Cdk5rap1"
                     /note="CDK5 regulatory subunit associated protein 1"
                     /db_xref="GeneID:252827"
                     /db_xref="RGD:708387"
     exon            1..14
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            15..328
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..1794
                     /gene="Cdk5rap1"
                     /EC_number="2.8.4.3"
                     /note="CDK5 activator-binding protein C42;
                     mt-tRNA-2-methylthio-N6-dimethylallyladenosine synthase;
                     mt-tRNA-N6-(dimethylallyl)adenosine(37)
                     methylthiotransferase"
                     /codon_start=1
                     /product="mitochondrial tRNA methylthiotransferase
                     CDK5RAP1"
                     /protein_id="NP_663773.1"
                     /db_xref="GeneID:252827"
                     /db_xref="RGD:708387"
                     /translation="MHPLQRVFRAQRLSAPLTSMCWVLLRTFRAHNSTSCPDPEGKSS
                     EGVQKDFSSRLATGPTFQHFLRSASVPQEKPSSPEVEDPPPYLSGDELLGRQRKVYLE
                     TYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLK
                     VLKAKRPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDLPRLLAVVESG
                     QQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPV
                     ASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFSSTGSANLSRGFTTNYKPKQGG
                     LRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRV
                     LEAMRRGYSREAYVALVHHIREAIPGVGLSSDFITGFCGETEDDHLQTVSLLREVQYN
                     TGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKVNATSVGCTQLVL
                     VEGFSKRSTTDLCGRNDANLKVIFPDAEVEDITDPGLKVRAQPGDYVLVKIISASSQT
                     LKGHILCRTTMKDSSMNCLT"
     exon            329..432
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            433..467
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            468..568
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            569..779
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            780..900
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1131
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1229
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1285
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1416
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1566
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1707
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1945
                     /gene="Cdk5rap1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcagttgtg ggagattaag gactgcaggt gccatgcatc ctttacagcg tgtcttccga
       61 gcacagaggc tatcagcacc attgacctcc atgtgctggg tgttgcttag gaccttcagg
      121 gcacataaca gcacttcctg tcctgatcca gaggggaaga gctcagaagg agttcagaag
      181 gatttcagct ccaggctagc cactggaccg acttttcagc attttttaag aagtgcttca
      241 gttcctcaag agaaaccatc ttctccagaa gtggaggacc cacctcccta tctctcaggg
      301 gatgaacttc taggaaggca gagaaaggtc tacctcgaga cctatggctg tcagatgaat
      361 gtgaatgaca cagagatagc ctggtccatt ttacagaaga gcggctactt gcggaccagc
      421 aaccttcaag aggccgatgt gatccttctt gtcacgtgtt ctatcaggga gaaggctgag
      481 cagaccatct ggaaccgttt acatcagcta aaagtcctga aggcaaagcg accacgctcc
      541 cgggtacctc tgaggattgg gattctaggc tgcatggctg agagactgaa gggagagatc
      601 ctcaacaggg agaaaatggt agatcttttg gctggtccag atgcctatcg agaccttccc
      661 cggctgctgg ccgttgtgga gtcaggtcag caagcagcaa atgtgcttct ctctctggat
      721 gagacctacg cggatatcat gccagtccaa acgagcccca gtgccacttc tgccttcgtg
      781 tcaatcatgc ggggctgcga caatatgtgc agttactgta tcgttccttt cactcggggc
      841 agggagagga gtcggccagt tgcctccatt ctagatgaag tgaggaagct ctctgagcag
      901 gggctaaaag aagtgacact tctaggtcag aatgttaata gttttcggga caattcagaa
      961 gtccagttca gtagtacagg gtctgccaac ctcagccgtg gctttactac caactataaa
     1021 cccaaacaag gagggcttcg tttttctcac cttctggatc aggtttcaag aatagatcct
     1081 gaaatgagga ttcgtttcac ctctcctcac cccaaggatt ttcccgatga ggttctacag
     1141 ctgattcgtg agagacacaa catctgtaag cagatccacc tgccagccca gagtgggagc
     1201 agccgtgtac tggaggccat gcggagagga tattcaagag aagcatacgt ggctttggtt
     1261 catcatatca gggaggctat cccaggtgtg ggcctcagca gcgacttcat cactggcttc
     1321 tgtggagaga cagaggacga tcacctgcag acagtgtctt tacttcgaga agttcagtac
     1381 aatactggtt ttctctttgc atacagcatg aggcaaaaga cacgagcata tcataggctg
     1441 aaggatgacg ttccagaaga agtaaaatta aggcgtttgg aggaacttat tactgtcttc
     1501 cgagaagaag cttcaaaagt caatgcgacc tcagtgggtt gtacccagct ggtattggtt
     1561 gaagggttca gcaagcgttc taccacagac ctgtgtggcc gaaatgatgc aaaccttaag
     1621 gtgattttcc ctgatgccga ggtggaggat atcactgacc ctgggctcaa ggtcagagct
     1681 cagcctgggg actatgtgct ggtgaagatc atctctgcca gttctcaaac tctcaaagga
     1741 cacattctct gcaggaccac tatgaaagac tcatcaatga attgcttgac ctgagttggt
     1801 ggcttagtgg tggacttggg cagttttttc tcatgctttg gagacaaggt aaagtcttga
     1861 agcatcttcg caccaaatgg aagagtacat ttctctgttg tcactcaaac aactgttcca
     1921 ataataaatt tcatgaatta atggcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
//
