LOCUS       NM_001105897            2521 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus chaperonin containing TCP1 subunit 8 (Cct8),
            mRNA.
ACCESSION   NM_001105897 XM_001067000 XM_213673
VERSION     NM_001105897.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Freund,A., Zhong,F.L., Venteicher,A.S., Meng,Z., Veenstra,T.D.,
            Frydman,J. and Artandi,S.E.
  TITLE     Proteostatic control of telomerase function through TRiC-mediated
            folding of TCAB1
  JOURNAL   Cell 159 (6), 1389-1403 (2014)
   PUBMED   25467444
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2521)
  AUTHORS   Slater,L.H., Hett,E.C., Clatworthy,A.E., Mark,K.G. and Hung,D.T.
  TITLE     CCT chaperonin complex is required for efficient delivery of
            anthrax toxin into the cytosol of host cells
  JOURNAL   Proc Natl Acad Sci U S A 110 (24), 9932-9937 (2013)
   PUBMED   23716698
REFERENCE   4  (bases 1 to 2521)
  AUTHORS   Knee,K.M., Sergeeva,O.A. and King,J.A.
  TITLE     Human TRiC complex purified from HeLa cells contains all eight CCT
            subunits and is active in vitro
  JOURNAL   Cell Stress Chaperones 18 (2), 137-144 (2013)
   PUBMED   23011926
REFERENCE   5  (bases 1 to 2521)
  AUTHORS   Dun,M.D., Smith,N.D., Baker,M.A., Lin,M., Aitken,R.J. and Nixon,B.
  TITLE     The chaperonin containing TCP1 complex (CCT/TRiC) is involved in
            mediating sperm-oocyte interaction
  JOURNAL   J Biol Chem 286 (42), 36875-36887 (2011)
   PUBMED   21880732
REFERENCE   6  (bases 1 to 2521)
  AUTHORS   Seo,S., Baye,L.M., Schulz,N.P., Beck,J.S., Zhang,Q., Slusarski,D.C.
            and Sheffield,V.C.
  TITLE     BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family
            chaperonins and mediate BBSome assembly
  JOURNAL   Proc Natl Acad Sci U S A 107 (4), 1488-1493 (2010)
   PUBMED   20080638
REFERENCE   7  (bases 1 to 2521)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 2521)
  AUTHORS   Hu,Y.H., Warnatz,H.J., Vanhecke,D., Wagner,F., Fiebitz,A.,
            Thamm,S., Kahlem,P., Lehrach,H., Yaspo,M.L. and Janitz,M.
  TITLE     Cell array-based intracellular localization screening reveals novel
            functional features of human chromosome 21 proteins
  JOURNAL   BMC Genomics 7, 155 (2006)
   PUBMED   16780588
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2521)
  AUTHORS   Kubota,H., Hynes,G. and Willison,K.
  TITLE     The eighth Cct gene, Cctq, encoding the theta subunit of the
            cytosolic chaperonin containing TCP-1
  JOURNAL   Gene 154 (2), 231-236 (1995)
   PUBMED   7890169
REFERENCE   10 (bases 1 to 2521)
  AUTHORS   Hynes,G., Kubota,H. and Willison,K.R.
  TITLE     Antibody characterisation of two distinct conformations of the
            chaperonin-containing TCP-1 from mouse testis
  JOURNAL   FEBS Lett 358 (2), 129-132 (1995)
   PUBMED   7828721
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DY312304.1, CV076369.1, CO400743.1, DY319674.1 and
            JAXUCZ010000011.1.
            
            On Sep 27, 2011 this sequence version replaced NM_001105897.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.2741075.1,
                                           SRR22399489.434031.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-508               DY312304.1         1-508
            509-1068            CV076369.1         87-646
            1069-1411           CO400743.1         262-604
            1412-1696           DY319674.1         105-389
            1697-2521           JAXUCZ010000011.1  40196619-40197443   c
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q11"
     gene            1..2521
                     /gene="Cct8"
                     /note="chaperonin containing TCP1 subunit 8"
                     /db_xref="GeneID:288305"
                     /db_xref="RGD:1305452"
     exon            1..187
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..1774
                     /gene="Cct8"
                     /note="chaperonin subunit 8 (theta); chaperonin containing
                     Tcp1, subunit 8 (theta)"
                     /codon_start=1
                     /product="T-complex protein 1 subunit theta"
                     /protein_id="NP_001099367.2"
                     /db_xref="GeneID:288305"
                     /db_xref="RGD:1305452"
                     /translation="MALHVPKAPGFAQMLKDGAKHFSGLEEAVYRNIQACKELAQTTR
                     TAYGPNGMNKMVINRLEKLFVTNDAATILRELEVQHPAAKMIVMASHMQEQEVGDGTN
                     FVLVFAGALLELAEELLRIGLSVSEVITGYEIACKKAHEILPDLVCCSAKNLRDVDEV
                     SSLLRTSIMSKQYGSEEFLAKLISQACVSIFPDSGNFNVDNIRVCKILGSGVYSSSVL
                     HGMVFKKETEGDVTSVKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLM
                     DAQVKAIAGTGANVIVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALP
                     KLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAV
                     DDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPR
                     ALAENSGVKANEVISKLYSVHQEGNKNVGLDIEAEVPAVKDMLEASILDTYLGKYWAI
                     KLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND"
     exon            188..278
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            279..358
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            359..508
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            509..689
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            690..751
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            752..889
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1068
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1135
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1223
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1339
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1411
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1576
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1696
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..2521
                     /gene="Cct8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgaaggggc ggggcgaccg gccatagaag accgtgaggc agcgcaaagg caccttccca
       61 gcaggctttg cgcggggcac cttgcttcct tggagcgagc gcgtgagcgg ttgattgcgg
      121 gctagccatg gcgcttcacg tccccaaggc cccgggcttt gcccagatgc tcaaagatgg
      181 agcaaaacat ttctcaggat tagaagaggc tgtgtataga aatatacagg cttgcaagga
      241 gctggcccag accacccgta cagcatatgg accaaatgga atgaataaaa tggtgattaa
      301 tcgcctggag aagttgtttg tgaccaacga tgcagcgact attttaagag agctagaagt
      361 gcagcatcct gctgccaaga tgattgtgat ggcctctcat atgcaagaac aagaggttgg
      421 cgatggcaca aactttgttc tggtattcgc tggcgctctt ctggaactag ctgaagaact
      481 tctgaggatt ggcctgtcag tatcagaggt aataacaggg tatgaaatag cttgcaaaaa
      541 agctcatgag attcttcctg atttggtgtg ttgttctgcc aaaaacctgc gagatgttga
      601 tgaggtgtcg tcgctgctgc gcacctccat aatgagtaag cagtacggca gcgaggagtt
      661 tctggccaag ctcatttctc aggcgtgtgt atctattttt cctgattctg gcaatttcaa
      721 tgttgataac atcagagtat gtaagattct gggctctggt gtttattcat cctcagtatt
      781 acatggcatg gtttttaaga aggaaacgga aggcgatgtg acatctgtca aagatgcaaa
      841 aatagccgtg tactcttgtc cctttgatgg catgataaca gagacaaagg gaactgtgct
      901 gattaagact gctgaagaac tgatgaactt tagtaaagga gaagagaatc ttatggatgc
      961 acaagtgaaa gccattgcag gcactggtgc aaatgtcata gtgacaggcg gcaaagtggc
     1021 agatatggct cttcattatg ctaacaagta caatatcatg ctggtgagac tgaactcaaa
     1081 gtgggatctc agacgactct gtaaaacagt tggtgccaca gctcttccaa aattgactcc
     1141 tcccgtccta gaagaaatgg gacattgtga cagtgtttac ctctcagaag ttggagatac
     1201 ccaggtggtt gtttttaagc atgaaaaaga agatggtgcc atttctacca tagttcttcg
     1261 aggttctaca gacaatctga tggatgatat agaaagggca gtagatgacg gagttaatac
     1321 tttcaaagtt ctcacaaggg ataagcgtct tgtacctgga ggtggagcta cagaaatcga
     1381 attggccaaa caaatcacat catatggaga aacgtgtcct ggtcttgaac agtatgctat
     1441 taagaaattc gctgaagcat ttgaagctat tccacgggca ctggcagaaa attctggcgt
     1501 caaggccaat gaagttatct ctaaactcta ttcagtacac caagaaggaa acaaaaatgt
     1561 ggggttggat attgaggctg aagttcctgc tgtaaaggat atgttagaag ccagtatttt
     1621 agatacttac ttgggaaaat actgggctat taaactggcc actaatgctg cagtcactgt
     1681 acttagagtg gatcagatca tcatggcaaa accagctggt gggcccaagc ctccaagtgg
     1741 gaagaaagac tgggatgatg accaaaatga ctgaggaact ttgtcatggt gaagagttgt
     1801 gtttgtagag tagaacttgc ccaatgtttt cttacttttc ttaaagtatt tgtgtttgtt
     1861 ctctgctggg tggtacaata aacgtgttac tgttctggcc atctgattaa ctactgcttg
     1921 tgtcaatgtc aagttactca catgactgtt ctcattatag atctggactg cttctaaaag
     1981 caaactgcta tgaccatacc atgtatctat gttggcagtt gcattctaca agggacagac
     2041 agtttgaata gttgtaacag agacctaaga ggctagaaat cgtttctcat ttccaaagtt
     2101 atttttccaa tcccagtctg gatttaagct ccagtaacct gatacagtgg cttataaatt
     2161 ggtttgtcac catgccaaag ttcataagct tttcaataat cactggctga cttttcacaa
     2221 ggttttgcag cataatgtct agagtcacac tgacaccagg acttcacatc gcttccagca
     2281 ctacagtagt gaaacatgat gctgccagta gggttacagt aataacttaa aaatttaata
     2341 aagcacgttt tgggaatatc actttattga tgtgtcaaga actaccaccc agtcagttta
     2401 gttattcaaa tctttcctaa agttgaaact agaaagagta aaaaccagta tgctaataca
     2461 aaaactgctg taagttaatt ttgtctgtat ttaaataaac atgatttttt tctgtcattt
     2521 a
//
