LOCUS       NM_001033716            4466 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus calpastatin (Cast), transcript variant 3, mRNA.
ACCESSION   NM_001033716
VERSION     NM_001033716.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Gritsyna,Y.V., Salmov,N.N., Bobylev,A.G., Ulanova,A.D.,
            Kukushkin,N.I., Podlubnaya,Z.A. and Vikhlyantsev,I.M.
  TITLE     Increased Autolysis of mu-Calpain in Skeletal Muscles of Chronic
            Alcohol-Fed Rats
  JOURNAL   Alcohol Clin Exp Res 41 (10), 1686-1694 (2017)
   PUBMED   28800153
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Manfredi,L.H., Lustrino,D., Machado,J., Silveira,W.A., Zanon,N.M.,
            Navegantes,L.C. and Kettelhut,I.C.
  TITLE     Adrenodemedullation activates the Ca2+-dependent proteolysis in
            soleus muscles from rats exposed to cold
  JOURNAL   J Appl Physiol (1985) 122 (2), 317-326 (2017)
   PUBMED   27765842
REFERENCE   3  (bases 1 to 4466)
  AUTHORS   Mochida,S., Hida,Y., Tanifuji,S., Hagiwara,A., Hamada,S., Abe,M.,
            Ma,H., Yasumura,M., Kitajima,I., Sakimura,K. and Ohtsuka,T.
  TITLE     SAD-B Phosphorylation of CAST Controls Active Zone Vesicle
            Recycling for Synaptic Depression
  JOURNAL   Cell Rep 16 (11), 2901-2913 (2016)
   PUBMED   27626661
REFERENCE   4  (bases 1 to 4466)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4466)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 4466)
  AUTHORS   Yasuoka,H., Sugano,Y., Oka,T., Watanabe,C., Kaneko,Y., Nojima,T.,
            Matsumura,M., Fujii,T. and Mimori,T.
  TITLE     [Antigenic epitopes recognized by autoantibodies to calpastatin in
            patients with rheumatoid arthritis and their clinical significance]
  JOURNAL   Ryumachi 37 (3), 458-466 (1997)
   PUBMED   9256029
REFERENCE   7  (bases 1 to 4466)
  AUTHORS   Despres,N., Talbot,G., Plouffe,B., Boire,G. and Menard,H.A.
  TITLE     Detection and expression of a cDNA clone that encodes a polypeptide
            containing two inhibitory domains of human calpastatin and its
            recognition by rheumatoid arthritis sera
  JOURNAL   J Clin Invest 95 (4), 1891-1896 (1995)
   PUBMED   7706496
REFERENCE   8  (bases 1 to 4466)
  AUTHORS   Lee,W.J., Hatanaka,M. and Maki,M.
  TITLE     Multiple forms of rat calpastatin cDNA in the coding region of
            functionally unknown amino-terminal domain
  JOURNAL   Biochim Biophys Acta 1129 (2), 251-253 (1992)
   PUBMED   1730065
REFERENCE   9  (bases 1 to 4466)
  AUTHORS   Ishida,S., Emori,Y. and Suzuki,K.
  TITLE     Rat calpastatin has diverged primary sequence from other mammalian
            calpastatins but retains functionally important sequences
  JOURNAL   Biochim Biophys Acta 1088 (3), 436-438 (1991)
   PUBMED   2015306
REFERENCE   10 (bases 1 to 4466)
  AUTHORS   Uemori,T., Shimojo,T., Asada,K., Asano,T., Kimizuka,F., Kato,I.,
            Maki,M., Hatanaka,M., Murachi,T., Hanzawa,H. et al.
  TITLE     Characterization of a functional domain of human calpastatin
  JOURNAL   Biochem Biophys Res Commun 166 (3), 1485-1493 (1990)
   PUBMED   2407243
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC091239.1, AF346597.1 and Y13589.1.
            
            Transcript Variant: This variant (3) lacks two in-frame exons
            compared to variant 1. The resulting isoform (c) has the same N-
            and C-termini but is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ186625.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-319               BC091239.1         1-319
            320-903             AF346597.1         1-584
            904-1404            Y13589.1           597-1097
            1405-4466           BC091239.1         1474-4535
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q11"
     gene            1..4466
                     /gene="Cast"
                     /note="calpastatin"
                     /db_xref="GeneID:25403"
                     /db_xref="RGD:2278"
     exon            1..222
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..2106
                     /gene="Cast"
                     /note="isoform c is encoded by transcript variant 3;
                     calpain inhibitor"
                     /codon_start=1
                     /product="calpastatin isoform c"
                     /protein_id="NP_001028888.1"
                     /db_xref="GeneID:25403"
                     /db_xref="RGD:2278"
                     /translation="MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGS
                     DEVTASSAATGTSPRMSTTGAKAVKIESEKSQSSEPPVIHEKKPKGKPKEGSETKFQD
                     APSADGESVAGGVTVATASDKVVVKSGVNAALDDLIDTLGECEDTNKDDPPYTGPVVL
                     DPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAIDALSSDFTC
                     SSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGT
                     RQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEE
                     TSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDP
                     EDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPL
                     SDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRD
                     KELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLL
                     DNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSK
                     ASKNEEKTKDSSKKTEEVPKPKVDEDAT"
     exon            223..285
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            286..345
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            346..384
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            385..438
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            439..528
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            529..627
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            628..708
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            709..747
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            748..834
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            835..924
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1026
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1110
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1158
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1254
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1356
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1440
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1491
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1563
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1662
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1767
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1860
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1861..1896
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..1989
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2058
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2120
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2121..4441
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggggagctg gcccgggctc agggctgggt cggaaaagct gcctcactgc gccgttccgc
       61 tccaccgccc cgagctctgg cctgggcctc ccgtacaatt tctctgcgct ttcccagagg
      121 ctgcactcgc agcagtttca ccaaataatg tcccggcccg gcccgaagcc cgccgcctct
      181 tccaggcccc ggcgcggggc ggcagctagc cacacccagg agcatgttaa tgaaaagaat
      241 attggatcat cttctaaacc agcagaaaag aaaggatctg atgaggtgac agcttcctct
      301 gctgccactg gcacatctcc cagaatgagt accacaggag ctaaggctgt gaaaatagag
      361 tctgagaagt cacaatcaag tgagccacct gtgattcatg agaaaaagcc aaaaggaaag
      421 ccaaaggaag ggtcagagac aaaatttcaa gatgctcctt ctgctgatgg agagagtgtg
      481 gctggaggtg tgactgtggc cactgcctca gacaaggtgg ttgttaagtc aggcgtgaat
      541 gctgctttgg atgacctgat agacacctta ggtgaatgtg aagatactaa caaagatgat
      601 ccaccatata ctggaccagt agttctggac ccaatggatt ctacctacct tgaggcactg
      661 ggtataaaag aagggactat tcctccagag tataggaaac ttctggagaa aaatgaagct
      721 atcacaggac ctcttccaga ttctcctaaa cctatgggaa tcgaccatgc tatagatgcc
      781 ttgtcatctg atttcacctg tagctctcca actggaaagc aaactgagaa agagaagtct
      841 actggggaaa gttcgaaagc tcagtctgca ggagtgacca gaagtgctgt tcctccccag
      901 gagaagaaaa ggaaagtaga agaggaggtc atgaatgatc aagccctgca ggctctgtca
      961 gattcactgg gcacccggca gccagaccct cagagccatc ttaggcaagc caaacaagtc
     1021 aaagaggcaa aagcaaaaga agaaaggcag gagaaatgtg gtgaagatga ggacacagta
     1081 cctgctgagt acaggttaaa accagccaag gataaagatg gaaaaccact attgccagaa
     1141 cctgaagaaa catctaagtg cctgagtgag tcagagctga ttggggagct ttcagcagat
     1201 tttgtccaac ctacatatca agaaaaacca tctatgccag ctgcaaaaat aaagaagggc
     1261 gtagtgccag atgatgctgt agaaaccttg gctagaagcc tggggacaag gaaagaagat
     1321 ccagaagatg aaaaatctct ggtggataaa gtcaaggaga aagctaaaga agaagatcat
     1381 gaaaaacttg gtgaaaaaga agaaacaatt cctcctgatt atcgactaga aatagtcaag
     1441 gataaggatg gaaaaccgct cctgccaaaa gaagccgagg aacaacttcc acccttaagc
     1501 gatgacttcc ttcttgatgc attgtctcag gacttctcca gtcctgcaaa catattgtct
     1561 cttggttttg aagatgccaa actttctgct gctgtttctg aaacagtttc ccaggtgcct
     1621 gctccaagca accacacagc agccccaccg cctggcactg agaggagaga caaagaactc
     1681 gatgatgcct tggatgaact ttctgacagt cttggacaaa gacagcctga tccagatgag
     1741 aacaaaccac tagatgataa agtaaaggaa aaaatcaaag cagagcatag cgagaaactg
     1801 ggagaaagag acgacaccat cccccctgaa tataggcatc tcctggataa tgatgggaag
     1861 gacaaaccag agaagccact gacaaagaac acagagaagc ctggtcagga ccaggacccc
     1921 attgatgccc tctcagaaga cttggatagc tgccccccaa ctacagagac ttcacagaat
     1981 acaacaaagg agaagggtaa gaagacttca agttccaaag catccaagaa tgaagagaaa
     2041 acaaaggatt cctccaagaa gacagaggaa gtgcccaagc caaaggtgga tgaagatgca
     2101 acataaagtt tatgcatttt ggtatctgaa taaaaattct tcagctggtg gatggtgact
     2161 tttgaagatc aaaggctttg atgacaattt ttctgggtgg cttctagaca gttgtatttg
     2221 ttatgtcttt tgacacctta aacattgagt tgatattctt ttccagaaca aaaaagaatt
     2281 tgtctggttc atctgtgttc tgtattgagt attgataaac ttcaaagatt tttaattgcc
     2341 tttaggtagg agaaggaagc tttatatttg taaaaaatat atttgacagt ttcttaaaac
     2401 accaaaaatc ccagaaaacc tttgcaaaca tttgcacatt atatacatag tgcctgtaaa
     2461 actcattagt aattttggtt tacacccaat ttttttagca attgcaatat aatgctctta
     2521 tagtttgtca atattttgat ttcttattat gtctttttct tgggctttga tgtatctttg
     2581 ggttaatgtt gataagtcaa atgtggaata ttgtactttg ggtgtataac tcctttggag
     2641 atgctaataa taaattaatg atagataacc caggtaaacc ttgggagcac caatctcctt
     2701 tcctacctga actctccttt cctaggaatg aggacttcta ctcatggtca gacaagacac
     2761 attgagattt gaactaaaca gaaaaatgag gcacagactc gagtttaaat tttagcaata
     2821 aactggggat tcttagaata ctcatatgcc ttgaactatc ctggtttacc atgacattaa
     2881 actgtggatt ccactctcta catgaaaaac aaattgtaat cctttgctta tagccattcg
     2941 ccacattgtc taaagcatct ggctttatgg aagtcaaatt accaaacaca aaaatgaact
     3001 cttttaagta agttgtgtgt gtgtgtgtgt gtgtgtgtgt atacaatgta tacactgaaa
     3061 tttaaaatct gctgctatat caaagcaggt taaattatct taacatcttt gaatggtatc
     3121 agatttttct gagcactaca ctggtatcat tgatggaaat ttgataaaca attccatttc
     3181 tatttcctgg aaataaatgc tggaaaagat gtcaggtaca tataaacccg acttcaactt
     3241 tcttatttcc agaaatcaca gccttcgttg ccatcaattt aaaggtttta ttaggattta
     3301 cttaacttta ctaagcataa cttacattat gaggacagaa aagacagggg acagataaca
     3361 caaaggaaaa ctaaaaatat tcataatgaa cacattttgt taactgtctc taaaagtact
     3421 ctctacaaat catgcatgta ccttagattt caaatgctac ccacaaaagt ttttaattcc
     3481 cagtcctata aaaaggacag tcactattct gtttttaagg cactgacaag agaagaatgt
     3541 actcactgtc ttagttaggg ttttactgct atgaacagac accatggcca aggcaactct
     3601 tataagggca gcatttaatt ggggctggct tacaggttca gaggttcaat tcattatcat
     3661 caaggtggga gcatggcagc atccagatag gcatgatgta gggagaaact gagcatgcta
     3721 catcttgttc ctaaggcaaa caagagaaga ctggcttcca gggagctgga tgagtgtctt
     3781 acagcacatg cccgcactga tgacacactt ctctaacaaa gccactccta tttcaacaaa
     3841 tctactgcat gtgccaagca tattcaaaca atcatactac ctgtattttg aactcagtgt
     3901 ctaatccttg tatcttggca gtgtaggtat ctgtttgctc tttttgtgtc taaaagcacg
     3961 ctgaaaactt tgacgttggc agatatgctt ccaagtctat gactgtaata tgcacacttc
     4021 catgcacaca taggaatatg tttcaatgat gttacaaaat acataagttt aagagtgcct
     4081 ataaaattgc atacatactt aaggtaccaa aaatattttt ttaattaaga atattctcaa
     4141 gactttagtg tttgctggta actcacagtg acttgcataa gccattagga tctaaaggaa
     4201 tcctttttat tcttttctac tgtttacttt taaaattcta gtcaggtgaa aacaactgca
     4261 gatattgcct tagaagtaaa gaacaggtgg ttttcattac taaaagttgc tgcatatgta
     4321 atattttatc tgtttagaaa atgggctttc caaaagtgat ggtgaccaaa acaaaaatca
     4381 gtatttgtgt ttccttaatc aagattaaaa tgtaattaaa ggaaaatatt ttacataaaa
     4441 aaaaaaaaaa aaaaaaaaaa aaaaaa
//
