LOCUS       NM_001033715            4535 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus calpastatin (Cast), transcript variant 2, mRNA.
ACCESSION   NM_001033715
VERSION     NM_001033715.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Gritsyna,Y.V., Salmov,N.N., Bobylev,A.G., Ulanova,A.D.,
            Kukushkin,N.I., Podlubnaya,Z.A. and Vikhlyantsev,I.M.
  TITLE     Increased Autolysis of mu-Calpain in Skeletal Muscles of Chronic
            Alcohol-Fed Rats
  JOURNAL   Alcohol Clin Exp Res 41 (10), 1686-1694 (2017)
   PUBMED   28800153
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Manfredi,L.H., Lustrino,D., Machado,J., Silveira,W.A., Zanon,N.M.,
            Navegantes,L.C. and Kettelhut,I.C.
  TITLE     Adrenodemedullation activates the Ca2+-dependent proteolysis in
            soleus muscles from rats exposed to cold
  JOURNAL   J Appl Physiol (1985) 122 (2), 317-326 (2017)
   PUBMED   27765842
REFERENCE   3  (bases 1 to 4535)
  AUTHORS   Mochida,S., Hida,Y., Tanifuji,S., Hagiwara,A., Hamada,S., Abe,M.,
            Ma,H., Yasumura,M., Kitajima,I., Sakimura,K. and Ohtsuka,T.
  TITLE     SAD-B Phosphorylation of CAST Controls Active Zone Vesicle
            Recycling for Synaptic Depression
  JOURNAL   Cell Rep 16 (11), 2901-2913 (2016)
   PUBMED   27626661
REFERENCE   4  (bases 1 to 4535)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4535)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 4535)
  AUTHORS   Yasuoka,H., Sugano,Y., Oka,T., Watanabe,C., Kaneko,Y., Nojima,T.,
            Matsumura,M., Fujii,T. and Mimori,T.
  TITLE     [Antigenic epitopes recognized by autoantibodies to calpastatin in
            patients with rheumatoid arthritis and their clinical significance]
  JOURNAL   Ryumachi 37 (3), 458-466 (1997)
   PUBMED   9256029
REFERENCE   7  (bases 1 to 4535)
  AUTHORS   Despres,N., Talbot,G., Plouffe,B., Boire,G. and Menard,H.A.
  TITLE     Detection and expression of a cDNA clone that encodes a polypeptide
            containing two inhibitory domains of human calpastatin and its
            recognition by rheumatoid arthritis sera
  JOURNAL   J Clin Invest 95 (4), 1891-1896 (1995)
   PUBMED   7706496
REFERENCE   8  (bases 1 to 4535)
  AUTHORS   Lee,W.J., Hatanaka,M. and Maki,M.
  TITLE     Multiple forms of rat calpastatin cDNA in the coding region of
            functionally unknown amino-terminal domain
  JOURNAL   Biochim Biophys Acta 1129 (2), 251-253 (1992)
   PUBMED   1730065
REFERENCE   9  (bases 1 to 4535)
  AUTHORS   Ishida,S., Emori,Y. and Suzuki,K.
  TITLE     Rat calpastatin has diverged primary sequence from other mammalian
            calpastatins but retains functionally important sequences
  JOURNAL   Biochim Biophys Acta 1088 (3), 436-438 (1991)
   PUBMED   2015306
REFERENCE   10 (bases 1 to 4535)
  AUTHORS   Uemori,T., Shimojo,T., Asada,K., Asano,T., Kimizuka,F., Kato,I.,
            Maki,M., Hatanaka,M., Murachi,T., Hanzawa,H. et al.
  TITLE     Characterization of a functional domain of human calpastatin
  JOURNAL   Biochem Biophys Res Commun 166 (3), 1485-1493 (1990)
   PUBMED   2407243
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC091239.1.
            
            Transcript Variant: This variant (2) lacks an in-frame exon
            compared to variant 1. The resulting isoform (b) has the same N-
            and C-termini but is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC091239.1, DQ186627.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4535              BC091239.1         1-4535
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q11"
     gene            1..4535
                     /gene="Cast"
                     /note="calpastatin"
                     /db_xref="GeneID:25403"
                     /db_xref="RGD:2278"
     exon            1..222
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..2175
                     /gene="Cast"
                     /note="isoform b is encoded by transcript variant 2;
                     calpain inhibitor"
                     /codon_start=1
                     /product="calpastatin isoform b"
                     /protein_id="NP_001028887.1"
                     /db_xref="GeneID:25403"
                     /db_xref="RGD:2278"
                     /translation="MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGS
                     DEVTASSAATGTSPRMSTTGAKAVKIESEKSQSSEPPVIHEKKPKGKPKEGSETKFQD
                     APSADGESVAGGVTVATASDKVVVKKKEKKSLTPTLPMESTLNKLSDKSGVNAALDDL
                     IDTLGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGP
                     LPDSPKPMGIDHAIDALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEK
                     KRKVEEEVMNDQALQALSDSLGTRQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTV
                     PAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIK
                     KGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRL
                     EIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSE
                     TVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKI
                     KAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDS
                     CPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT"
     exon            223..285
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            286..345
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            346..384
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            385..438
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            439..528
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            529..597
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            598..696
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            697..777
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            778..816
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            817..903
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            904..993
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1095
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1179
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1227
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1323
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1425
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1509
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1560
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1632
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..1731
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1836
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1929
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..1965
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2058
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2127
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2189
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..4510
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggggagctg gcccgggctc agggctgggt cggaaaagct gcctcactgc gccgttccgc
       61 tccaccgccc cgagctctgg cctgggcctc ccgtacaatt tctctgcgct ttcccagagg
      121 ctgcactcgc agcagtttca ccaaataatg tcccggcccg gcccgaagcc cgccgcctct
      181 tccaggcccc ggcgcggggc ggcagctagc cacacccagg agcatgttaa tgaaaagaat
      241 attggatcat cttctaaacc agcagaaaag aaaggatctg atgaggtgac agcttcctct
      301 gctgccactg gcacatctcc cagaatgagt accacaggag ctaaggctgt gaaaatagag
      361 tctgagaagt cacaatcaag tgagccacct gtgattcatg agaaaaagcc aaaaggaaag
      421 ccaaaggaag ggtcagagac aaaatttcaa gatgctcctt ctgctgatgg agagagtgtg
      481 gctggaggtg tgactgtggc cactgcctca gacaaggtgg ttgttaagaa aaaagagaag
      541 aaatcattaa cacctacttt acctatggag tccacactaa acaagctatc tgacaagtca
      601 ggcgtgaatg ctgctttgga tgacctgata gacaccttag gtgaatgtga agatactaac
      661 aaagatgatc caccatatac tggaccagta gttctggacc caatggattc tacctacctt
      721 gaggcactgg gtataaaaga agggactatt cctccagagt ataggaaact tctggagaaa
      781 aatgaagcta tcacaggacc tcttccagat tctcctaaac ctatgggaat cgaccatgct
      841 atagatgcct tgtcatctga tttcacctgt agctctccaa ctggaaagca aactgagaaa
      901 gagaagtcta ctggggaaag ttcgaaagct cagtctgcag gagtgaccag aagtgctgtt
      961 cctccccagg agaagaaaag gaaagtagaa gaggaggtca tgaatgatca agccctgcag
     1021 gctctgtcag attcactggg cacccggcag ccagaccctc agagccatct taggcaagcc
     1081 aaacaagtca aagaggcaaa agcaaaagaa gaaaggcagg agaaatgtgg tgaagatgag
     1141 gacacagtac ctgctgagta caggttaaaa ccagccaagg ataaagatgg aaaaccacta
     1201 ttgccagaac ctgaagaaac atctaagtgc ctgagtgagt cagagctgat tggggagctt
     1261 tcagcagatt ttgtccaacc tacatatcaa gaaaaaccat ctatgccagc tgcaaaaata
     1321 aagaagggcg tagtgccaga tgatgctgta gaaaccttgg ctagaagcct ggggacaagg
     1381 aaagaagatc cagaagatga aaaatctctg gtggataaag tcaaggagaa agctaaagaa
     1441 gaagatcatg aaaaacttgg tgaaaaagaa gaaacaattc ctcctgatta tcgactagaa
     1501 atagtcaagg ataaggatgg aaaaccgctc ctgccaaaag aagccgagga acaacttcca
     1561 cccttaagcg atgacttcct tcttgatgca ttgtctcagg acttctccag tcctgcaaac
     1621 atattgtctc ttggttttga agatgccaaa ctttctgctg ctgtttctga aacagtttcc
     1681 caggtgcctg ctccaagcaa ccacacagca gccccaccgc ctggcactga gaggagagac
     1741 aaagaactcg atgatgcctt ggatgaactt tctgacagtc ttggacaaag acagcctgat
     1801 ccagatgaga acaaaccact agatgataaa gtaaaggaaa aaatcaaagc agagcatagc
     1861 gagaaactgg gagaaagaga cgacaccatc ccccctgaat ataggcatct cctggataat
     1921 gatgggaagg acaaaccaga gaagccactg acaaagaaca cagagaagcc tggtcaggac
     1981 caggacccca ttgatgccct ctcagaagac ttggatagct gccccccaac tacagagact
     2041 tcacagaata caacaaagga gaagggtaag aagacttcaa gttccaaagc atccaagaat
     2101 gaagagaaaa caaaggattc ctccaagaag acagaggaag tgcccaagcc aaaggtggat
     2161 gaagatgcaa cataaagttt atgcattttg gtatctgaat aaaaattctt cagctggtgg
     2221 atggtgactt ttgaagatca aaggctttga tgacaatttt tctgggtggc ttctagacag
     2281 ttgtatttgt tatgtctttt gacaccttaa acattgagtt gatattcttt tccagaacaa
     2341 aaaagaattt gtctggttca tctgtgttct gtattgagta ttgataaact tcaaagattt
     2401 ttaattgcct ttaggtagga gaaggaagct ttatatttgt aaaaaatata tttgacagtt
     2461 tcttaaaaca ccaaaaatcc cagaaaacct ttgcaaacat ttgcacatta tatacatagt
     2521 gcctgtaaaa ctcattagta attttggttt acacccaatt tttttagcaa ttgcaatata
     2581 atgctcttat agtttgtcaa tattttgatt tcttattatg tctttttctt gggctttgat
     2641 gtatctttgg gttaatgttg ataagtcaaa tgtggaatat tgtactttgg gtgtataact
     2701 cctttggaga tgctaataat aaattaatga tagataaccc aggtaaacct tgggagcacc
     2761 aatctccttt cctacctgaa ctctcctttc ctaggaatga ggacttctac tcatggtcag
     2821 acaagacaca ttgagatttg aactaaacag aaaaatgagg cacagactcg agtttaaatt
     2881 ttagcaataa actggggatt cttagaatac tcatatgcct tgaactatcc tggtttacca
     2941 tgacattaaa ctgtggattc cactctctac atgaaaaaca aattgtaatc ctttgcttat
     3001 agccattcgc cacattgtct aaagcatctg gctttatgga agtcaaatta ccaaacacaa
     3061 aaatgaactc ttttaagtaa gttgtgtgtg tgtgtgtgtg tgtgtgtgta tacaatgtat
     3121 acactgaaat ttaaaatctg ctgctatatc aaagcaggtt aaattatctt aacatctttg
     3181 aatggtatca gatttttctg agcactacac tggtatcatt gatggaaatt tgataaacaa
     3241 ttccatttct atttcctgga aataaatgct ggaaaagatg tcaggtacat ataaacccga
     3301 cttcaacttt cttatttcca gaaatcacag ccttcgttgc catcaattta aaggttttat
     3361 taggatttac ttaactttac taagcataac ttacattatg aggacagaaa agacagggga
     3421 cagataacac aaaggaaaac taaaaatatt cataatgaac acattttgtt aactgtctct
     3481 aaaagtactc tctacaaatc atgcatgtac cttagatttc aaatgctacc cacaaaagtt
     3541 tttaattccc agtcctataa aaaggacagt cactattctg tttttaaggc actgacaaga
     3601 gaagaatgta ctcactgtct tagttagggt tttactgcta tgaacagaca ccatggccaa
     3661 ggcaactctt ataagggcag catttaattg gggctggctt acaggttcag aggttcaatt
     3721 cattatcatc aaggtgggag catggcagca tccagatagg catgatgtag ggagaaactg
     3781 agcatgctac atcttgttcc taaggcaaac aagagaagac tggcttccag ggagctggat
     3841 gagtgtctta cagcacatgc ccgcactgat gacacacttc tctaacaaag ccactcctat
     3901 ttcaacaaat ctactgcatg tgccaagcat attcaaacaa tcatactacc tgtattttga
     3961 actcagtgtc taatccttgt atcttggcag tgtaggtatc tgtttgctct ttttgtgtct
     4021 aaaagcacgc tgaaaacttt gacgttggca gatatgcttc caagtctatg actgtaatat
     4081 gcacacttcc atgcacacat aggaatatgt ttcaatgatg ttacaaaata cataagttta
     4141 agagtgccta taaaattgca tacatactta aggtaccaaa aatatttttt taattaagaa
     4201 tattctcaag actttagtgt ttgctggtaa ctcacagtga cttgcataag ccattaggat
     4261 ctaaaggaat cctttttatt cttttctact gtttactttt aaaattctag tcaggtgaaa
     4321 acaactgcag atattgcctt agaagtaaag aacaggtggt tttcattact aaaagttgct
     4381 gcatatgtaa tattttatct gtttagaaaa tgggctttcc aaaagtgatg gtgaccaaaa
     4441 caaaaatcag tatttgtgtt tccttaatca agattaaaat gtaattaaag gaaaatattt
     4501 tacataaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
//
