LOCUS       NM_017118               1405 bp    mRNA    linear   ROD 04-DEC-2024
DEFINITION  Rattus norvegicus calpain, small subunit 1 (Capns1), mRNA.
ACCESSION   NM_017118 XM_341824
VERSION     NM_017118.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Tyagi,T., Ahmad,S., Gupta,N., Sahu,A., Ahmad,Y., Nair,V.,
            Chatterjee,T., Bajaj,N., Sengupta,S., Ganju,L., Singh,S.B. and
            Ashraf,M.Z.
  TITLE     Altered expression of platelet proteins and calpain activity
            mediate hypoxia-induced prothrombotic phenotype
  JOURNAL   Blood 123 (8), 1250-1260 (2014)
   PUBMED   24297866
  REMARK    GeneRIF: This study suggests that augmented calpain activity is
            associated with increased incidence of thrombosis under hypoxic
            environments.
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   3  (bases 1 to 1405)
  AUTHORS   Hanna,R.A., Campbell,R.L. and Davies,P.L.
  TITLE     Calcium-bound structure of calpain and its mechanism of inhibition
            by calpastatin
  JOURNAL   Nature 456 (7220), 409-412 (2008)
   PUBMED   19020623
REFERENCE   4  (bases 1 to 1405)
  AUTHORS   Moldoveanu,T., Gehring,K. and Green,D.R.
  TITLE     Concerted multi-pronged attack by calpastatin to occlude the
            catalytic cleft of heterodimeric calpains
  JOURNAL   Nature 456 (7220), 404-408 (2008)
   PUBMED   19020622
REFERENCE   5  (bases 1 to 1405)
  AUTHORS   Ozaki,T., Yamashita,T. and Ishiguro,S.
  TITLE     ERp57-associated mitochondrial mu-calpain truncates
            apoptosis-inducing factor
  JOURNAL   Biochim Biophys Acta 1783 (10), 1955-1963 (2008)
   PUBMED   18559257
REFERENCE   6  (bases 1 to 1405)
  AUTHORS   Arthur,J.S., Elce,J.S., Hegadorn,C., Williams,K. and Greer,P.A.
  TITLE     Disruption of the murine calpain small subunit gene, Capn4: calpain
            is essential for embryonic development but not for cell growth and
            division
  JOURNAL   Mol Cell Biol 20 (12), 4474-4481 (2000)
   PUBMED   10825211
REFERENCE   7  (bases 1 to 1405)
  AUTHORS   Hosfield,C.M., Elce,J.S., Davies,P.L. and Jia,Z.
  TITLE     Crystal structure of calpain reveals the structural basis for
            Ca(2+)-dependent protease activity and a novel mode of enzyme
            activation
  JOURNAL   EMBO J 18 (24), 6880-6889 (1999)
   PUBMED   10601010
REFERENCE   8  (bases 1 to 1405)
  AUTHORS   Blanchard,H., Grochulski,P., Li,Y., Arthur,J.S., Davies,P.L.,
            Elce,J.S. and Cygler,M.
  TITLE     Structure of a calpain Ca(2+)-binding domain reveals a novel
            EF-hand and Ca(2+)-induced conformational changes
  JOURNAL   Nat Struct Biol 4 (7), 532-538 (1997)
   PUBMED   9228945
REFERENCE   9  (bases 1 to 1405)
  AUTHORS   Sorimachi,H., Amano,S., Ishiura,S. and Suzuki,K.
  TITLE     Primary sequences of rat mu-calpain large and small subunits are,
            respectively, moderately and highly similar to those of human
  JOURNAL   Biochim Biophys Acta 1309 (1-2), 37-41 (1996)
   PUBMED   8950173
REFERENCE   10 (bases 1 to 1405)
  AUTHORS   Graham-Siegenthaler,K., Gauthier,S., Davies,P.L. and Elce,J.S.
  TITLE     Active recombinant rat calpain II. Bacterially produced large and
            small subunits associate both in vivo and in vitro
  JOURNAL   J Biol Chem 269 (48), 30457-30460 (1994)
   PUBMED   7982961
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC098068.1.
            
            On Jun 13, 2005 this sequence version replaced XM_341824.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098068.1, SRR8487227.166058.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..1405
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /note="calpain, small subunit 1"
                     /db_xref="GeneID:29156"
                     /db_xref="RGD:2270"
     exon            1..38
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            39..268
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..866
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /EC_number="3.4.22.17"
                     /note="CDPS; CSS1; CANP small subunit; calpain regulatory
                     subunit; calcium-dependent protease small subunit 1;
                     calcium-activated neutral proteinase small subunit;
                     calpain 4; calpain small subunit 1-like"
                     /codon_start=1
                     /product="calpain small subunit 1"
                     /protein_id="NP_058814.1"
                     /db_xref="GeneID:29156"
                     /db_xref="RGD:2270"
                     /translation="MFLVNSFLKGGGGGGGGGGLGGGLGNVLGGLISGAAGGGGGGGG
                     GGGMGLGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRSHYSNIEANESEEERQ
                     FRKLFVQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGK
                     LGFEEFKYLWNNIKKWQGIYKRFDTDRSGTIGSNELPGAFEAAGFHLNQHIYSMIIRR
                     YSDETGNMDFDNFISCLVRLDAMFRAFRSLDKNGTGQIQVNIQEWLQLTMYS"
     exon            269..302
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            303..392
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            393..450
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            451..515
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            516..584
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            585..663
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            664..780
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            781..839
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
     exon            840..1375
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccagcggcgg gcgagcaaga ccgcggaccg ccggatccga ctctgctgca aacatgttct
       61 tggtgaattc gttcttgaag ggcggcggtg gcggtggggg aggcgggggt ctgggcgggg
      121 gcctgggcaa tgtgctcgga ggcctgatca gcggagctgc tggaggcggc ggcggtggag
      181 gcggcggtgg cggcatggga ctgggaggag gtggcggagg cggtggaaca gccatgcgta
      241 tcctgggcgg agtcattagc gccatcagcg aggctgctgc gcagtacaac ccggagcccc
      301 cgcccccacg tagccattac tctaatattg aggccaacga gagtgaagag gaacgtcagt
      361 tcaggaaact ttttgtccag ctggctggag acgacatgga ggtcagcgct acagaactca
      421 tgaatatcct caacaaggtc gtgacccgac acccagatct gaaaactgat gggttcggca
      481 ttgacacttg tcggagcatg gtggctgtta tggatagtga caccacaggc aagctgggct
      541 ttgaggagtt caagtacctc tggaacaaca ttaaaaagtg gcagggtata tacaaacgct
      601 ttgatactga ccgatcaggg accattggta gcaatgaact cccaggggcc tttgaggcag
      661 caggattcca cctgaatcag catatctaca gcatgatcat ccgccgctac tcggacgaga
      721 ccgggaacat ggatttcgat aacttcatca gctgcttggt caggctggat gccatgttcc
      781 gtgctttcag atctcttgac aaaaatggca ctggacagat ccaagtgaac atccaggagt
      841 ggttgcagct gactatgtat tcctgagtgg aagtcacaga cctgccgcct cactgctcac
      901 tgtggagcct tgaacctgat cctgtctgca gccacgtttt tacggtcatg tcctgctacc
      961 cagaggcctt ccttttccct atgctggtac ccagcttccc agtcggcagc cagagctcaa
     1021 cgtgccccag cttgctgcac cctgagttcc cctggctctg agacaagcta gcttgctctg
     1081 ccgaagtgtc accccacccc agcacaccca gtccgcaccc tctgtgacct ccatcctgtc
     1141 tgtgtgccaa gcctagcact cctattgctt ccatgtccca agggccctgt ctcagttctg
     1201 ggaggatcac gcctgtccct acacaccttg ttacacactc acacagttac ctcccaggca
     1261 gccaaataaa ctccaggcca tccagtgcca cacaccctag tgcctatgcc tgcactgttt
     1321 tccccctgca cagctgtcgg gcccaagagg aaataaactc tccattcgct gcttcaaaaa
     1381 aaaaaaaaaa aaaaaaaaaa aaaaa
//
