LOCUS       NP_058814                270 aa            linear   ROD 04-DEC-2024
DEFINITION  calpain small subunit 1 [Rattus norvegicus].
ACCESSION   NP_058814 XP_341825
VERSION     NP_058814.1
DBSOURCE    REFSEQ: accession NM_017118.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 270)
  AUTHORS   Tyagi,T., Ahmad,S., Gupta,N., Sahu,A., Ahmad,Y., Nair,V.,
            Chatterjee,T., Bajaj,N., Sengupta,S., Ganju,L., Singh,S.B. and
            Ashraf,M.Z.
  TITLE     Altered expression of platelet proteins and calpain activity
            mediate hypoxia-induced prothrombotic phenotype
  JOURNAL   Blood 123 (8), 1250-1260 (2014)
   PUBMED   24297866
  REMARK    GeneRIF: This study suggests that augmented calpain activity is
            associated with increased incidence of thrombosis under hypoxic
            environments.
REFERENCE   2  (residues 1 to 270)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   3  (residues 1 to 270)
  AUTHORS   Hanna,R.A., Campbell,R.L. and Davies,P.L.
  TITLE     Calcium-bound structure of calpain and its mechanism of inhibition
            by calpastatin
  JOURNAL   Nature 456 (7220), 409-412 (2008)
   PUBMED   19020623
REFERENCE   4  (residues 1 to 270)
  AUTHORS   Moldoveanu,T., Gehring,K. and Green,D.R.
  TITLE     Concerted multi-pronged attack by calpastatin to occlude the
            catalytic cleft of heterodimeric calpains
  JOURNAL   Nature 456 (7220), 404-408 (2008)
   PUBMED   19020622
REFERENCE   5  (residues 1 to 270)
  AUTHORS   Ozaki,T., Yamashita,T. and Ishiguro,S.
  TITLE     ERp57-associated mitochondrial mu-calpain truncates
            apoptosis-inducing factor
  JOURNAL   Biochim Biophys Acta 1783 (10), 1955-1963 (2008)
   PUBMED   18559257
REFERENCE   6  (residues 1 to 270)
  AUTHORS   Arthur,J.S., Elce,J.S., Hegadorn,C., Williams,K. and Greer,P.A.
  TITLE     Disruption of the murine calpain small subunit gene, Capn4: calpain
            is essential for embryonic development but not for cell growth and
            division
  JOURNAL   Mol Cell Biol 20 (12), 4474-4481 (2000)
   PUBMED   10825211
REFERENCE   7  (residues 1 to 270)
  AUTHORS   Hosfield,C.M., Elce,J.S., Davies,P.L. and Jia,Z.
  TITLE     Crystal structure of calpain reveals the structural basis for
            Ca(2+)-dependent protease activity and a novel mode of enzyme
            activation
  JOURNAL   EMBO J 18 (24), 6880-6889 (1999)
   PUBMED   10601010
REFERENCE   8  (residues 1 to 270)
  AUTHORS   Blanchard,H., Grochulski,P., Li,Y., Arthur,J.S., Davies,P.L.,
            Elce,J.S. and Cygler,M.
  TITLE     Structure of a calpain Ca(2+)-binding domain reveals a novel
            EF-hand and Ca(2+)-induced conformational changes
  JOURNAL   Nat Struct Biol 4 (7), 532-538 (1997)
   PUBMED   9228945
REFERENCE   9  (residues 1 to 270)
  AUTHORS   Sorimachi,H., Amano,S., Ishiura,S. and Suzuki,K.
  TITLE     Primary sequences of rat mu-calpain large and small subunits are,
            respectively, moderately and highly similar to those of human
  JOURNAL   Biochim Biophys Acta 1309 (1-2), 37-41 (1996)
   PUBMED   8950173
REFERENCE   10 (residues 1 to 270)
  AUTHORS   Graham-Siegenthaler,K., Gauthier,S., Davies,P.L. and Elce,J.S.
  TITLE     Active recombinant rat calpain II. Bacterially produced large and
            small subunits associate both in vivo and in vitro
  JOURNAL   J Biol Chem 269 (48), 30457-30460 (1994)
   PUBMED   7982961
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC098068.1.
            
            On Jun 13, 2005 this sequence version replaced XP_341825.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098068.1, SRR8487227.166058.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     Protein         1..270
                     /product="calpain small subunit 1"
                     /EC_number="3.4.22.17"
                     /note="CDPS; CSS1; CANP small subunit; calpain regulatory
                     subunit; calcium-dependent protease small subunit 1;
                     calcium-activated neutral proteinase small subunit;
                     calpain 4; calpain small subunit 1-like"
                     /calculated_mol_wt=28439
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:P04632; propagated from
                     UniProtKB/Swiss-Prot (Q64537.3)"
     Site            6
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P04632; propagated from
                     UniProtKB/Swiss-Prot (Q64537.3)"
     Region          102..270
                     /region_name="EFh_PEF_CPNS1_2"
                     /note="Penta-EF hand, calcium binding motifs, found in
                     calcium-dependent protease small subunit CAPNS1 and
                     CAPNS2; cd16188"
                     /db_xref="CDD:320063"
     Region          102..130
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320063"
     Site            order(114,121,139,156,158,165,184,186,188,195,227,
                     229..230)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320063"
     Site            order(129,170,173..174,177,228)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:320063"
     Site            order(144..145,147,157,159..160,211,214,217..220,222,230,
                     234,237..238,241..242,245,254..261,263..270)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:320063"
     Region          144..174
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320063"
     Region          175..205
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320063"
     Site            181
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P04632; propagated from
                     UniProtKB/Swiss-Prot (Q64537.3)"
     Region          211..239
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320063"
     Region          240..270
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320063"
     CDS             1..270
                     /gene="Capns1"
                     /gene_synonym="Capn4"
                     /coded_by="NM_017118.1:54..866"
                     /db_xref="GeneID:29156"
                     /db_xref="RGD:2270"
ORIGIN      
        1 mflvnsflkg gggggggggl ggglgnvlgg lisgaagggg gggggggmgl ggggggggta
       61 mrilggvisa iseaaaqynp epppprshys nieaneseee rqfrklfvql agddmevsat
      121 elmnilnkvv trhpdlktdg fgidtcrsmv avmdsdttgk lgfeefkylw nnikkwqgiy
      181 krfdtdrsgt igsnelpgaf eaagfhlnqh iysmiirrys detgnmdfdn fisclvrlda
      241 mfrafrsldk ngtgqiqvni qewlqltmys
//
