LOCUS       NM_017117               3137 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus calpain 3 (Capn3), mRNA.
ACCESSION   NM_017117
VERSION     NM_017117.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Baburuna,Y., Sotnikova,L. and Krestinina,O.
  TITLE     Identification of Phosphorylated Calpain 3 in Rat Brain
            Mitochondria under mPTP Opening
  JOURNAL   Int J Mol Sci 22 (19), 10613 (2021)
   PUBMED   34638951
  REMARK    GeneRIF: Identification of Phosphorylated Calpain 3 in Rat Brain
            Mitochondria under mPTP Opening.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Wu,R., Wang,J., Yao,J., Dong,Z., Liu,Y. and Liu,M.
  TITLE     MEF2A regulates Calpain 3 expression in L6 myoblasts
  JOURNAL   Gene 668, 204-210 (2018)
   PUBMED   29783071
  REMARK    GeneRIF: MEF2A is a transcriptional regulator for Capn3 gene
            expression.
REFERENCE   3  (bases 1 to 3137)
  AUTHORS   Wu,R., Yan,Y., Yao,J., Liu,Y., Zhao,J. and Liu,M.
  TITLE     Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy
            and Regeneration Following Sciatic Nerve Injury in Rats
  JOURNAL   Int J Mol Sci 16 (11), 26927-26935 (2015)
   PUBMED   26569227
  REMARK    GeneRIF: the expression pattern of the active CAPN3 protein is
            linked to muscle atrophy and regeneration following denervation
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3137)
  AUTHORS   Tao,T., Shi,H., Guan,Y., Huang,D., Chen,Y., Lane,D.P., Chen,J. and
            Peng,J.
  TITLE     Def defines a conserved nucleolar pathway that leads p53 to
            proteasome-independent degradation
  JOURNAL   Cell Res 23 (5), 620-634 (2013)
   PUBMED   23357851
REFERENCE   5  (bases 1 to 3137)
  AUTHORS   Pistoni,M., Shiue,L., Cline,M.S., Bortolanza,S., Neguembor,M.V.,
            Xynos,A., Ares,M. Jr. and Gabellini,D.
  TITLE     Rbfox1 downregulation and altered calpain 3 splicing by FRG1 in a
            mouse model of Facioscapulohumeral muscular dystrophy (FSHD)
  JOURNAL   PLoS Genet 9 (1), e1003186 (2013)
   PUBMED   23300487
REFERENCE   6  (bases 1 to 3137)
  AUTHORS   Ma,H., Shih,M., Hata,I., Fukiage,C., Azuma,M. and Shearer,T.R.
  TITLE     Protein for Lp82 calpain is expressed and enzymatically active in
            young rat lens
  JOURNAL   Exp Eye Res 67 (2), 221-229 (1998)
   PUBMED   9733588
REFERENCE   7  (bases 1 to 3137)
  AUTHORS   Ono,Y., Shimada,H., Sorimachi,H., Richard,I., Saido,T.C.,
            Beckmann,J.S., Ishiura,S. and Suzuki,K.
  TITLE     Functional defects of a muscle-specific calpain, p94, caused by
            mutations associated with limb-girdle muscular dystrophy type 2A
  JOURNAL   J Biol Chem 273 (27), 17073-17078 (1998)
   PUBMED   9642272
REFERENCE   8  (bases 1 to 3137)
  AUTHORS   Ma,H., Fukiage,C., Azuma,M. and Shearer,T.R.
  TITLE     Cloning and expression of mRNA for calpain Lp82 from rat lens:
            splice variant of p94
  JOURNAL   Invest Ophthalmol Vis Sci 39 (2), 454-461 (1998)
   PUBMED   9478008
REFERENCE   9  (bases 1 to 3137)
  AUTHORS   Richard,I., Brenguier,L., Dincer,P., Roudaut,C., Bady,B.,
            Burgunder,J.M., Chemaly,R., Garcia,C.A., Halaby,G., Jackson,C.E.,
            Kurnit,D.M., Lefranc,G., Legum,C., Loiselet,J., Merlini,L.,
            Nivelon-Chevallier,A., Ollagnon-Roman,E., Restagno,G., Topaloglu,H.
            and Beckmann,J.S.
  TITLE     Multiple independent molecular etiology for limb-girdle muscular
            dystrophy type 2A patients from various geographical origins
  JOURNAL   Am J Hum Genet 60 (5), 1128-1138 (1997)
   PUBMED   9150160
REFERENCE   10 (bases 1 to 3137)
  AUTHORS   Sorimachi,H., Imajoh-Ohmi,S., Emori,Y., Kawasaki,H., Ohno,S.,
            Minami,Y. and Suzuki,K.
  TITLE     Molecular cloning of a novel mammalian calcium-dependent protease
            distinct from both m- and mu-types. Specific expression of the mRNA
            in skeletal muscle
  JOURNAL   J Biol Chem 264 (33), 20106-20111 (1989)
   PUBMED   2555341
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Nov 26, 2020 this sequence version replaced NM_017117.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J05121.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760475
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-610               JAXUCZ010000003.1  127861590-127862199
            611-680             JAXUCZ010000003.1  127884189-127884258
            681-799             JAXUCZ010000003.1  127885524-127885642
            800-933             JAXUCZ010000003.1  127889242-127889375
            934-1102            JAXUCZ010000003.1  127889923-127890091
            1103-1246           JAXUCZ010000003.1  127891237-127891380
            1247-1330           JAXUCZ010000003.1  127893025-127893108
            1331-1416           JAXUCZ010000003.1  127895195-127895280
            1417-1494           JAXUCZ010000003.1  127897082-127897159
            1495-1655           JAXUCZ010000003.1  127897906-127898066
            1656-1825           JAXUCZ010000003.1  127900734-127900903
            1826-1837           JAXUCZ010000003.1  127901047-127901058
            1838-2046           JAXUCZ010000003.1  127901691-127901899
            2047-2083           JAXUCZ010000003.1  127902743-127902779
            2084-2101           JAXUCZ010000003.1  127905126-127905143
            2102-2215           JAXUCZ010000003.1  127907372-127907485
            2216-2293           JAXUCZ010000003.1  127908269-127908346
            2294-2351           JAXUCZ010000003.1  127908676-127908733
            2352-2416           JAXUCZ010000003.1  127908818-127908882
            2417-2485           JAXUCZ010000003.1  127909652-127909720
            2486-2564           JAXUCZ010000003.1  127909810-127909888
            2565-2681           JAXUCZ010000003.1  127910089-127910205
            2682-2740           JAXUCZ010000003.1  127910836-127910894
            2741-3137           JAXUCZ010000003.1  127911211-127911607
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q35"
     gene            1..3137
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /note="calpain 3"
                     /db_xref="GeneID:29155"
                     /db_xref="RGD:2269"
     exon            1..610
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     CDS             302..2767
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /EC_number="3.4.22.54"
                     /note="CANP 3; calpain L3; calpain p94; calcium-activated
                     neutral proteinase 3; muscle-specific calcium-activated
                     neutral protease 3"
                     /codon_start=1
                     /product="calpain-3"
                     /protein_id="NP_058813.2"
                     /db_xref="GeneID:29155"
                     /db_xref="RGD:2269"
                     /translation="MPTVISPTVAPRTGAEPRSPGPVPHPAQGKTTEAGGGHPGGIYS
                     AIISRNFPIIGVKEKTFEQLHKKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRP
                     PEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYA
                     GIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEA
                     LKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGT
                     SPSGLNMGELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGH
                     AYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVT
                     EDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNF
                     PDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYE
                     VPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQE
                     GEFILRVFSEKRNLSEEAENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQEAEEG
                     KDKTGPDKQGESPQPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVN
                     KHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSG
                     TINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHA
                     FDKDGDGIIKLNVLEWLQLTMYA"
     exon            611..680
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            681..799
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            800..933
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1102
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1246
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1330
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1416
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1494
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1655
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1825
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1837
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..2046
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2083
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2101
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2215
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2293
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2351
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2416
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2485
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2564
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2681
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2682..2740
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2741..3137
                     /gene="Capn3"
                     /gene_synonym="Lp82; Lp84; Lp85; p94"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttctcttttt ctcctctggc aagcctgctg ctggtaggca cccccaggta gaagctgcgt
       61 ctaaatcctt tattgcctct tcctcaggaa tacctattgc tctagggtca tagttcacct
      121 atttaagctg gtcagaggcc agccaatttt ctgataggat ttaaactttg aagagactgt
      181 agccattttt ttcctcagat gacagaatca cttcaacttc cactttgtaa tcgcttcctt
      241 tccttgaagg tagctgaatc ttgttttctt taaaaacgtc ttccttccaa agttgcctgc
      301 catgccgacc gttattagtc caactgtggc cccaaggaca ggagctgagc ccaggtcccc
      361 agggccagtt cctcacccag ctcaaggcaa gaccactgag gctggaggtg gacacccggg
      421 tggcatctat tcagccatca tcagccgcaa ttttccgatc attggtgtga aagagaagac
      481 attcgagcag ctccacaaga agtgcctaga gaagaaagtt ctttacctgg atcccgagtt
      541 cccaccggat gagacctctc tcttttacag ccagaagttc cccatccagt tcgtctggaa
      601 gagacctccg gaaatttgtg agaatccccg atttatcatt ggtggagcca acaggactga
      661 catctgccaa ggagatctag gggactgctg gtttcttgca gccattgcct gtctgaccct
      721 gaatgagcga ctgcttttcc gagttatacc tcatgatcaa agtttcactg aaaactacgc
      781 agggatcttc cacttccagt tctggcgcta tggagactgg gtagatgtgg ttattgacga
      841 ctgtctgccg acatacaaca accagctggt cttcaccaaa tccaaccacc gcaatgagtt
      901 ctggagtgct ctactggaga aagcatatgc caagctccat ggttcctatg aagctctgaa
      961 aggtgggaac accacagaag ccatggagga cttcacagga ggggtgacag agttttttga
     1021 gatcaaggat gctccgagtg acatgtacaa gatcatgagg aaagctatcg agagaggctc
     1081 cctcatgggc tgctccattg atgatggcac caacatgact tatggaacct ctccttctgg
     1141 tctgaacatg ggggaattga ttgcgcggat ggtgagaaat atggataact cgctgctcag
     1201 agactcagac ctggacccca gggcctcaga tgacagaccg tcacggacaa ttgttccggt
     1261 gcagtatgaa acaagaatgg cctgtggact ggtgaaaggg cacgcctatt cagtcactgg
     1321 gctggaggag gccctgttca aaggcgagaa ggtgaagctg gtgcggctgc ggaacccctg
     1381 gggccaggtg gagtggaacg gctcttggag tgatggttgg aaggactgga gctttgtgga
     1441 caaagacgag aaggcccgtc tgcagcacca ggtcaccgag gatggagagt tctggatgtc
     1501 atatgatgac tttgtctacc atttcacaaa gctggagatc tgcaacctca cagctgatgc
     1561 cctggagtcc gataagcttc agacctggac agtgtctgta aatgagggcc gctgggtgag
     1621 gggctgttct gctggaggct gccggaactt cccagacact ttctggacca acccgcagta
     1681 ccgtctcaag ctcctggagg aggatgatga ccctgatgac tctgaggtga tttgcagctt
     1741 cctcgtggct ctgatgcaga aaaatcggcg caaggaccgg aagctggggg ccaacctctt
     1801 caccattggc ttcgctatct acgaggttcc caaagagatg cacgggaata agcaacacct
     1861 gcagaaggac ttcttcttgt acaatgcctc caaggccagg agcaaaacct acatcaacat
     1921 gcgggaggtg tcccagcgct tccgcctgcc gcccagcgag tatgtcattg tcccctccac
     1981 ttacgagccc catcaggagg gggaattcat cctccgggtc ttctctgaaa agaggaatct
     2041 ctctgaggaa gctgagaata caatctctgt ggaccggcca gtgaaaaaga aaaaaaacaa
     2101 gcccatcatc ttcgtttcag acagagcaaa cagcaacaag gagctgggtg tggaccagga
     2161 ggcagaggag ggcaaagaca aaacagggcc ggataaacaa ggggaaagcc cacagccacg
     2221 gcctggccac acagaccagg agagtgagga gcagcagcaa ttccggaaca tcttcaggca
     2281 gattgcaggc gacgacatgg agatctgtgc ggatgaactc aagaatgtcc ttaatacggt
     2341 ggtgaacaaa cacaaggacc tgaagacaca agggttcact ctggagtcct gcagaagcat
     2401 gatagctctc atggatacag atggctctgg gagactgaat cttcaagagt tccatcacct
     2461 ctggaaaaag atcaaggcct ggcagaaaat cttcaaacac tatgacactg accattctgg
     2521 taccatcaat agctatgaga tgcgaaatgc agtcaatgat gcaggcttcc atctcaacag
     2581 ccaactctat gacatcatca ccatgcgcta cgcagacaaa cacatgaaca tcgactttga
     2641 cagcttcatc tgctgcttcg tcaggctgga agggatgttc agagcttttc acgcatttga
     2701 caaggatgga gatggcatca tcaaactgaa cgtacttgag tggctgcagc ttaccatgta
     2761 tgcctgaacc agatgacctc atgtaagatc aaccaggatt ccatctcaac acgacacagc
     2821 tagggctgtt taccacaagg aacccagtag gcacacctcc accaaactgg gctcctggtc
     2881 acgttccttc tccactttga cccaagtctt ggtgcacagc cacctcaagt gtctggcttg
     2941 ctgggagctc tgcagacgct gtctacatag cttgtaactg ggttgtccac agccctgtca
     3001 ccatctgcac tcagttctgc cagttttagg gtgggtctac tctggggtcc atagggtgtg
     3061 gatacctgac aaaaatgtgg ctacattctg aaagaatcta tctaaataaa ggcacgcaca
     3121 tggctggttc caccatt
//
