LOCUS       NM_017116               3556 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus calpain 2 (Capn2), mRNA.
ACCESSION   NM_017116
VERSION     NM_017116.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Pestereva,N., Ivleva,I., Zubov,A., Tikhomirova,M. and Karpenko,M.
  TITLE     m-Calpain is released from striatal synaptosomes
  JOURNAL   Int J Neurosci 133 (2), 215-221 (2023)
   PUBMED   33688783
  REMARK    GeneRIF: m-Calpain is released from striatal synaptosomes.
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Zhang,S., Qiu,Y., Feng,Y., Zhang,Y., Li,Y., Wang,B., Wei,H.,
            Chen,X., Shen,L., Li,W., Zheng,L. and Zhang,Y.
  TITLE     Calpain-2 Facilitates Autophagic/Lysosomal Defects and Apoptosis in
            ARPE-19 Cells and Rats Induced by Exosomes from RPE Cells under
            NaIO3 Stimulation
  JOURNAL   Oxid Med Cell Longev 2023, 3310621 (2023)
   PUBMED   36703913
  REMARK    GeneRIF: Calpain-2 Facilitates Autophagic/Lysosomal Defects and
            Apoptosis in ARPE-19 Cells and Rats Induced by Exosomes from RPE
            Cells under NaIO3 Stimulation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3556)
  AUTHORS   Zaman,V., Drasites,K.P., Myatich,A., Shams,R., Shields,D.C.,
            Matzelle,D., Haque,A. and Banik,N.L.
  TITLE     Inhibition of Calpain Attenuates Degeneration of Substantia Nigra
            Neurons in the Rotenone Rat Model of Parkinson's Disease
  JOURNAL   Int J Mol Sci 23 (22), 13849 (2022)
   PUBMED   36430329
  REMARK    GeneRIF: Inhibition of Calpain Attenuates Degeneration of
            Substantia Nigra Neurons in the Rotenone Rat Model of Parkinson's
            Disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3556)
  AUTHORS   Ge,X.Y., Zhu,T.T., Yao,M.Z., Liu,H., Wu,Q., Qiao,J., Zhang,W.F. and
            Hu,C.P.
  TITLE     MicroRNA-137 Inhibited Hypoxia-Induced Proliferation of Pulmonary
            Artery Smooth Muscle Cells by Targeting Calpain-2
  JOURNAL   Biomed Res Int 2021, 2202888 (2021)
   PUBMED   34513987
  REMARK    GeneRIF: MicroRNA-137 Inhibited Hypoxia-Induced Proliferation of
            Pulmonary Artery Smooth Muscle Cells by Targeting Calpain-2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3556)
  AUTHORS   Wang,W.Y., Xie,L., Zou,X.S., Li,N., Yang,Y.G., Wu,Z.J., Tian,X.Y.,
            Zhao,G.Y. and Chen,M.H.
  TITLE     Inhibition of extracellular signal-regulated kinase/calpain-2
            pathway reduces neuroinflammation and necroptosis after cerebral
            ischemia-reperfusion injury in a rat model of cardiac arrest
  JOURNAL   Int Immunopharmacol 93, 107377 (2021)
   PUBMED   33517223
  REMARK    GeneRIF: Inhibition of extracellular signal-regulated
            kinase/calpain-2 pathway reduces neuroinflammation and necroptosis
            after cerebral ischemia-reperfusion injury in a rat model of
            cardiac arrest.
REFERENCE   6  (bases 1 to 3556)
  AUTHORS   Dourdin,N., Brustis,J.J., Balcerzak,D., Elamrani,N., Poussard,S.,
            Cottin,P. and Ducastaing,A.
  TITLE     Myoblast fusion requires fibronectin degradation by exteriorized
            m-calpain
  JOURNAL   Exp Cell Res 235 (2), 385-394 (1997)
   PUBMED   9299163
REFERENCE   7  (bases 1 to 3556)
  AUTHORS   Arthur,J.S., Gauthier,S. and Elce,J.S.
  TITLE     Active site residues in m-calpain: identification by site-directed
            mutagenesis
  JOURNAL   FEBS Lett 368 (3), 397-400 (1995)
   PUBMED   7635186
REFERENCE   8  (bases 1 to 3556)
  AUTHORS   Balcerzak,D., Poussard,S., Brustis,J.J., Elamrani,N., Soriano,M.,
            Cottin,P. and Ducastaing,A.
  TITLE     An antisense oligodeoxyribonucleotide to m-calpain mRNA inhibits
            myoblast fusion
  JOURNAL   J Cell Sci 108 (Pt 5), 2077-2082 (1995)
   PUBMED   7657725
REFERENCE   9  (bases 1 to 3556)
  AUTHORS   Graham-Siegenthaler,K., Gauthier,S., Davies,P.L. and Elce,J.S.
  TITLE     Active recombinant rat calpain II. Bacterially produced large and
            small subunits associate both in vivo and in vitro
  JOURNAL   J Biol Chem 269 (48), 30457-30460 (1994)
   PUBMED   7982961
REFERENCE   10 (bases 1 to 3556)
  AUTHORS   DeLuca,C.I., Davies,P.L., Samis,J.A. and Elce,J.S.
  TITLE     Molecular cloning and bacterial expression of cDNA for rat calpain
            II 80 kDa subunit
  JOURNAL   Biochim Biophys Acta 1216 (1), 81-93 (1993)
   PUBMED   8218419
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC065306.1.
            
            On Jan 25, 2004 this sequence version replaced NM_017116.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065306.1, L09120.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q26"
     gene            1..3556
                     /gene="Capn2"
                     /note="calpain 2"
                     /db_xref="GeneID:29154"
                     /db_xref="RGD:2268"
     exon            1..663
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     CDS             427..2529
                     /gene="Capn2"
                     /EC_number="3.4.22.53"
                     /note="M-calpain; calpain M-type; calpain II;
                     millimolar-calpain; calpain-2 large subunit;
                     calcium-activated neutral proteinase 2; CANP 2; calpain 2,
                     (m/II) large subunit"
                     /codon_start=1
                     /product="calpain-2 catalytic subunit"
                     /protein_id="NP_058812.1"
                     /db_xref="GeneID:29154"
                     /db_xref="RGD:2268"
                     /translation="MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGA
                     LFQDPSFPALPSSLGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGAL
                     GDCWLLAAIASLTLNEEILARVVPLDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPT
                     KDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELR
                     KPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSG
                     SLQKLIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRH
                     YSRLEICNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLE
                     EEDEDDEDGERGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELTGQTNIHLSK
                     NFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFEPHKNGDFCIRVFSEKKAD
                     YQTVDDEIEANIEEIEANEEDIGDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRED
                     IKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNS
                     YEMRKALEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLEILFKIFKQLDPE
                     NTGTIQLDLISWLSFSVL"
     exon            664..733
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            734..852
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            853..986
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1155
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1239
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1325
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1400
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1561
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1731
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1743
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1955
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..1992
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2058
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2116
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2181
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2250
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2329
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2446
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2505
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..3513
                     /gene="Capn2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctgactcatt tgctgtttcc tggacagtca ttgctgagtt tcaccctgcc ttcttcccgt
       61 tgagtctcaa ctttcgcacc ccaacttgca agtgccctcc cccaccctcc tccacggcaa
      121 ccgggcctcg ccatcccggg atctgtccga aggtggcagc attggcccct cagtcctcca
      181 cggtcgggct gtcgctgtgc aggtcgccgg ctggccgcgt ccggagccca gcgccgagca
      241 ggcctggaga ggcggaggcc acaccccgcg cgcggcccag gcgcttcccg ccggtgaatc
      301 atccccgcag cggcagctcc cgcagtccgc tgcagcgccc cgggcttggc cgcgccccaa
      361 ccgagtgctg cgccccgcct ctccgcgacc cctctctctg tgctgtgcgc catcccgatc
      421 gctaccatgg cgggcatcgc gatgaaactg gccaaagacc gcgaggcggc cgaggggctg
      481 gggtctcacg agagagccat caagtacctc aaccaggact acgagacact gcggaacgag
      541 tgcctggagg ccggggcgct cttccaggat ccttccttcc ccgccctgcc gtcgtccctg
      601 ggcttcaagg agttggggcc ctactccagc aaaactcggg gcatcgaatg gaagcggccc
      661 acggagatct gcgcagaccc ccagttcatt attggaggtg ctacccgcac agacatctgc
      721 caaggagccc ttggggactg ctggcttctg gctgccatcg cctccctcac cttgaatgaa
      781 gaaatcctgg ctcgagttgt gcctctagac cagagcttcc aggaaaacta tgcaggcatc
      841 ttccatttcc agttctggca atatggcgaa tgggtggagg tggtggtgga cgacagactg
      901 cccactaagg acggggagct gctctttgtt cattcagctg aggggagcga gttctggagt
      961 gcccttctgg agaaggccta tgccaagatc aatgggtgct atgaagcact ctcagggggt
     1021 gccaccactg agggctttga agacttcaca ggaggcattg ccgagtggta tgagctgagg
     1081 aagcctcctc ccaatttgtt caagatcatc cagaaggctt tggagaaagg ttctctgctt
     1141 ggctgctcta tcgatatcac cagcgctgcg gattctgagg ccgttacgta ccagaagttg
     1201 gtgaaaggac atgcgtactc cgtcactgga gccgaggagg ttgaaagctc aggaagcctg
     1261 cagaaattga tacgtatcag gaacccctgg ggacaagtgg agtggaccgg gaagtggaat
     1321 gacaactgcc ccagctggaa cacggttgac ccagaagtaa gggcaaatct aacagaacgg
     1381 caggaggacg gagaattctg gatgtccttc agtgacttcc tgaggcacta ctctcgtcta
     1441 gaaatctgca acctgacccc ggacaccctc acctgtgact cctacaagaa gtggaaactc
     1501 accaagatgg atgggaactg gaggcgaggc tccactgcag ggggctgcag gaattaccca
     1561 aataccttct ggatgaaccc tcagtaccta atcaagctgg aggaagaaga tgaagatgat
     1621 gaggatgggg agaggggctg taccttcctg gtgggcctca tccagaagca tcggcggcgg
     1681 cagaggaaga tgggtgagga catgcacacc attggcttcg gcatctatga ggtcccagag
     1741 gagctaacag ggcagaccaa catccacctc agcaaaaact ttttcctgac aacccgagcc
     1801 agggagcggt cagatacctt catcaacctc cgggaggtcc tcaaccgctt caagctgccc
     1861 ccgggagaat atgtccttgt tccttccacc ttcgagcccc acaagaatgg cgatttctgc
     1921 atccgagtct tctcagagaa gaaggctgac taccaaactg tcgatgatga aatcgaggcc
     1981 aacattgaag agattgaagc caatgaggag gacattggag atggattcag aaggctgttt
     2041 gctcagctgg ctggagagga tgcggagatc tctgcctttg agctgcagac catcttgaga
     2101 agagttctag ccaagcgcga agacatcaag tcagatggct tcagcatcga gacctgtaag
     2161 atcatggtgg acatgctgga tgaagatggg agcggcaagc tggggctgaa ggagttctac
     2221 atcctctgga cgaagattca gaaataccaa aaaatttacc gagagatcga cgtggacagg
     2281 tcgggaacca tgaattccta tgagatgcgg aaggctttgg aagaagcagg tttcaagctg
     2341 ccctgtcaac ttcatcaagt catcgttgcc cggtttgcag atgacgaact catcatcgac
     2401 tttgacaact ttgtgcggtg tttggttcgg ctggaaatac tattcaagat attcaagcag
     2461 ctggaccctg agaacactgg aacgatacag ctcgacctta tctcgtggct gagtttttca
     2521 gtactctgaa gttacatgcc tgaagatttc tggtggaaga aaacagccaa tgcctacact
     2581 taaatacttt gtatctggac cttgaacgta cgggaacatt tacttcatcg ggtgattgta
     2641 actgaagctc taaccgtcag aggtggatga actttcacat atcagagtaa tggatctgca
     2701 taccattaac caaatgcaaa tgaattgcat aaccctcggt aaggtcagag agagctttat
     2761 gcctgtacat agtatatcct ttctacttct acacatcgtc tcttcatagc aatattaaac
     2821 caggaaggac tgttgagagg tttaagtaat tgggcaaaca gcaaattgat ctcagaggcc
     2881 acaatgcctt gatgggaata tttaaagaaa tttaaaaatt taagatgcag tggtggcatc
     2941 ttccacagca ggccagagag aaacagagag agagagagag agacttggaa atacaataat
     3001 aaagtaatct gtggaggcgt ctaaagaatc cagtctgatg gaggcatcta aagaatccag
     3061 tctgatggag gcatctaaag aatccaggag ccaggagtat ctgacacaag gccaacctgg
     3121 gctacctagc aagacctgat ctcaaaaaat aaatgtcaat taaaaaaaaa gaaggcatgt
     3181 ttccatcatt tggttctaac aaaagatggt ttttatacac ctttgtgaac agcaagccct
     3241 cttagactca agtccttgac catatttggc cttagacatt gaagtagtga gtagtgagat
     3301 catttgactg accaactgag ccaggtctgc catctgaaag aaaatcgtga tacaacccca
     3361 gggatggtgc ctacggctcg ggagaacagg gctcagaaga tccttcttaa aacaaccaag
     3421 ctggcttcgt ggtccgagga aaatgctagc aaccatacag acatactgcc ttatccactt
     3481 ccaaacgtac tgttaataaa gagttgcaat gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3541 aaaaaaaaaa aaaaaa
//
