LOCUS       NM_031338               4769 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Rattus norvegicus calcium/calmodulin-dependent protein kinase
            kinase 2 (Camkk2), transcript variant 1, mRNA.
ACCESSION   NM_031338 XM_001079438
VERSION     NM_031338.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Li,C., Hao,J., Qiu,H. and Xin,H.
  TITLE     CaMKK2 alleviates myocardial ischemia/reperfusion injury by
            inhibiting oxidative stress and inflammation via the action on the
            AMPK-AKT-GSK-3beta/Nrf2 signaling cascade
  JOURNAL   Inflamm Res 72 (7), 1409-1425 (2023)
   PUBMED   37338678
  REMARK    GeneRIF: CaMKK2 alleviates myocardial ischemia/reperfusion injury
            by inhibiting oxidative stress and inflammation via the action on
            the AMPK-AKT-GSK-3beta/Nrf2 signaling cascade.
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Yang,J., Yu,J., Li,D., Yu,S., Ke,J., Wang,L., Wang,Y., Qiu,Y.,
            Gao,X., Zhang,J. and Huang,L.
  TITLE     Store-operated calcium entry-activated autophagy protects EPC
            proliferation via the CAMKK2-MTOR pathway in ox-LDL exposure
  JOURNAL   Autophagy 13 (1), 82-98 (2017)
   PUBMED   27791458
  REMARK    GeneRIF: the CAMKK2-MTOR pathway has a role in store-operated
            calcium entry-activated autophagy protection of endothelial
            progenitor cell proliferation in ox-LDL exposure
REFERENCE   3  (bases 1 to 4769)
  AUTHORS   Fujiwara,Y., Kawaguchi,Y., Fujimoto,T., Kanayama,N., Magari,M. and
            Tokumitsu,H.
  TITLE     Differential AMP-activated Protein Kinase (AMPK) Recognition
            Mechanism of Ca2+/Calmodulin-dependent Protein Kinase Kinase
            Isoforms
  JOURNAL   J Biol Chem 291 (26), 13802-13808 (2016)
   PUBMED   27151216
  REMARK    GeneRIF: Site-directed mutagenesis analysis revealed that Leu(358)
            in CaMKKbeta/Ile(322) in CaMKKalpha confer, at least in part, a
            distinct recognition of AMPK but not of CaMKIalpha.
REFERENCE   4  (bases 1 to 4769)
  AUTHORS   Liu,D.M., Wang,H.J., Han,B., Meng,X.Q., Chen,M.H., Yang,D.B.,
            Sun,Y., Li,Y.L. and Jiang,C.L.
  TITLE     CAMKK2, Regulated by Promoter Methylation, is a Prognostic Marker
            in Diffuse Gliomas
  JOURNAL   CNS Neurosci Ther 22 (6), 518-524 (2016)
   PUBMED   27012733
REFERENCE   5  (bases 1 to 4769)
  AUTHORS   Sinha,R.A., Singh,B.K., Zhou,J., Wu,Y., Farah,B.L., Ohba,K.,
            Lesmana,R., Gooding,J., Bay,B.H. and Yen,P.M.
  TITLE     Thyroid hormone induction of mitochondrial activity is coupled to
            mitophagy via ROS-AMPK-ULK1 signaling
  JOURNAL   Autophagy 11 (8), 1341-1357 (2015)
   PUBMED   26103054
REFERENCE   6  (bases 1 to 4769)
  AUTHORS   Hook,S.S. and Means,A.R.
  TITLE     Ca(2+)/CaM-dependent kinases: from activation to function
  JOURNAL   Annu Rev Pharmacol Toxicol 41, 471-505 (2001)
   PUBMED   11264466
  REMARK    Review article
REFERENCE   7  (bases 1 to 4769)
  AUTHORS   Sakagami,H., Umemiya,M., Saito,S. and Kondo,H.
  TITLE     Distinct immunohistochemical localization of two isoforms of
            Ca2+/calmodulin-dependent protein kinase kinases in the adult rat
            brain
  JOURNAL   Eur J Neurosci 12 (1), 89-99 (2000)
   PUBMED   10651863
REFERENCE   8  (bases 1 to 4769)
  AUTHORS   Anderson,K.A., Means,R.L., Huang,Q.H., Kemp,B.E., Goldstein,E.G.,
            Selbert,M.A., Edelman,A.M., Fremeau,R.T. and Means,A.R.
  TITLE     Components of a calmodulin-dependent protein kinase cascade.
            Molecular cloning, functional characterization and cellular
            localization of Ca2+/calmodulin-dependent protein kinase kinase
            beta
  JOURNAL   J Biol Chem 273 (48), 31880-31889 (1998)
   PUBMED   9822657
REFERENCE   9  (bases 1 to 4769)
  AUTHORS   Kitani,T., Okuno,S. and Fujisawa,H.
  TITLE     Molecular cloning of Ca2+/calmodulin-dependent protein kinase
            kinase beta
  JOURNAL   J Biochem 122 (1), 243-250 (1997)
   PUBMED   9276695
REFERENCE   10 (bases 1 to 4769)
  AUTHORS   Edelman,A.M., Mitchelhill,K.I., Selbert,M.A., Anderson,K.A.,
            Hook,S.S., Stapleton,D., Goldstein,E.G., Means,A.R. and Kemp,B.E.
  TITLE     Multiple Ca(2+)-calmodulin-dependent protein kinase kinases from
            rat brain. Purification, regulation by Ca(2+)-calmodulin, and
            partial amino acid sequence
  JOURNAL   J Biol Chem 271 (18), 10806-10810 (1996)
   PUBMED   8631893
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000228.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_031338.3.
            
            On Jul 29, 2021 this sequence version replaced NM_031338.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB018081.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132268
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                JACYVU010000228.1  2996503-2996573
            72-597              JACYVU010000228.1  3009260-3009785
            598-645             JACYVU010000228.1  3016863-3016910
            646-699             JACYVU010000228.1  3019303-3019356
            700-751             JACYVU010000228.1  3020139-3020190
            752-885             JACYVU010000228.1  3022813-3022946
            886-922             JACYVU010000228.1  3027081-3027117
            923-944             JACYVU010000228.1  3029677-3029698
            945-1033            JACYVU010000228.1  3029841-3029929
            1034-1233           JACYVU010000228.1  3031552-3031751
            1234-1287           JACYVU010000228.1  3032226-3032279
            1288-1361           JACYVU010000228.1  3035004-3035077
            1362-1449           JACYVU010000228.1  3035178-3035265
            1450-1578           JACYVU010000228.1  3036464-3036592
            1579-1679           JACYVU010000228.1  3040927-3041027
            1680-1722           JACYVU010000228.1  3041402-3041444
            1723-4769           JACYVU010000228.1  3044193-3047239
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q16"
     gene            1..4769
                     /gene="Camkk2"
                     /note="calcium/calmodulin-dependent protein kinase kinase
                     2"
                     /db_xref="GeneID:83506"
                     /db_xref="RGD:620092"
     exon            1..71
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            72..597
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1893
                     /gene="Camkk2"
                     /EC_number="2.7.11.17"
                     /note="isoform 1 is encoded by transcript variant 1;
                     calcium/calmodulin-dependent protein kinase 2 beta;
                     CaM-kinase kinase beta; caMKK beta; caM-KK beta;
                     calcium/calmodulin-dependent protein kinase kinase beta;
                     caMKK 2; caM-KK 2; caM-kinase kinase 2;
                     calcium/calmodulin-dependent protein kinase kinase 2,
                     beta; Ca+/Calmodulin-dependent protein kinase kinase beta
                     (CaM-kinase kinase beta)"
                     /codon_start=1
                     /product="calcium/calmodulin-dependent protein kinase
                     kinase 2 isoform 1"
                     /protein_id="NP_112628.2"
                     /db_xref="GeneID:83506"
                     /db_xref="RGD:620092"
                     /translation="MSSCVSSQPTSDRAAPQDELGSGGVSRESQKPCEALRGLSSLSI
                     HLGMESFIVVTECEPGRGVDLSLARDQPLEADGQELPLDASEPESRSLLSGRKMSLQE
                     RSQGGPASNSSLDMNGRCICPSLSYSPASSPQSSPRMPRRPTVESHHVSITGLQDCVQ
                     LNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRRPPPRGTRPA
                     PGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVME
                     VPTLKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSN
                     EFKGSDALLSNTVGTPAFMAPESLSETRKIFSGKALDVWAMGVTLYCFVFGQCPFMDE
                     RIMCLHSKIKSQALEFPDQPDIAEDLKDLITRMLDKNPESRIVVPEIKLHPWVTRHGA
                     EPLPSEDENCTLVEVTEEEVENSVKHIPSLATVILVKTMIRKRSFGNPFEGSRREERS
                     LSAPGNLLTKKPTREWEPLSEPKEARQRRQPPGPRASPCGGGGSALVKGGPCVESCGA
                     PAPGSPPRTPPQQPEEAMEPE"
     exon            598..645
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            646..699
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            700..751
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            752..885
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            886..922
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            923..944
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1033
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1233
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1287
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1361
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1449
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1578
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1679
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1722
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..4769
                     /gene="Camkk2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccggaccgag tcgagccgag ccaagccgag ccgggggcgc agagcgcggg aggcggcggc
       61 gcggagccca ggcagctcta ctgctgatgg aagtgctccg gcctggccga tgaagttggt
      121 acacacacca tgtcatcatg tgtctctagc cagcccacca gcgaccgggc ggccccccag
      181 gatgagctgg gaagcggggg tgtcagccgg gaaagccaga agccctgcga ggcactgcgg
      241 ggactctcat ccttaagcat ccacttgggc atggaatcct tcatcgtggt caccgagtgt
      301 gaaccaggcc ggggcgtgga cctcagcctg gccagagacc aacctctgga ggccgatggc
      361 caggaactcc ctctggatgc ctcggaacct gagtcacggt ccctgctttc tggtcgcaag
      421 atgtccctcc aggagcggtc ccagggcggg ccggcgtcca acagcagcct ggacatgaat
      481 ggacgctgca tctgcccatc cctgtcctac tcaccagcca gctccccaca gtcctctccc
      541 cggatgcccc ggcggcccac agtggagtcg caccacgtct ccatcacggg tttgcaggac
      601 tgtgtgcagc tgaatcagta cacgctgaag gatgaaattg gaaagggctc ctatggcgtt
      661 gtcaagctgg cctacaatga aaatgacaat acttattatg caatgaaagt gctgtccaaa
      721 aagaagctga tccgacaggc cggctttcca cgtcgccccc cacctcgagg tactcgccca
      781 gctccagggg gctgcatcca gcccaggggc cccattgagc aggtgtatca ggaaattgcc
      841 atcctcaaga agctggatca ccccaacgtg gtgaagctgg tggaggtcct ggatgaccct
      901 aacgaggacc atctgtacat ggtgtttgaa ctggtcaacc aagggcctgt gatggaagtt
      961 cccaccctca agccactgtc tgaagaccag gcccggttct acttccaaga tctgatcaaa
     1021 ggcattgagt acttacacta ccagaagatc attcaccggg acatcaaacc ttccaacctc
     1081 ctagtggggg aggacgggca catcaagata gccgacttcg gcgtgagcaa tgagttcaag
     1141 ggcagcgacg ccttgctgtc taacaccgtg ggcacgcctg ccttcatggc gcccgagtcg
     1201 ctctcagaga cccggaagat cttctccgga aaggccttgg atgtttgggc catgggtgtg
     1261 acactatact gctttgtttt tggccagtgc cctttcatgg atgaacgaat catgtgtttg
     1321 cacagtaaga tcaagagtca ggccctggag tttccagacc agcctgacat agccgaagac
     1381 ttgaaagatc tgatcacccg gatgttggac aagaacccag agtccaggat tgtggtacct
     1441 gaaatcaagc tgcacccttg ggtcacgagg cacggggccg agccactgcc gtcggaggac
     1501 gagaactgca cactggtcga ggtgaccgaa gaggaggtcg agaattcagt caaacacatt
     1561 cccagcctgg caactgtgat cctagtgaag accatgattc ggaaacggtc ttttgggaac
     1621 ccatttgaag gtagccggcg ggaggagcgt tccttgtcag cacccggaaa cctgctcacc
     1681 aaaaaaccaa ccagggagtg ggagcccttg tctgagccca aggaagcaag gcagcgaaga
     1741 cagcctccgg ggcccagagc cagcccctgt gggggaggag gaagtgctct tgtgaaaggt
     1801 ggtccctgcg tggaaagttg cggggctccg gcccctggct ccccgccacg cacgcctcca
     1861 cagcagcccg aggaggccat ggagccggag tagctgcgcg gaccacctga cctcgcatgc
     1921 ctggctgtgt ggccctgcac ggggctgctg caccctgcgt ttccatagca gcatgtccta
     1981 cggaaaccaa gcacgtgtgt agagccttga ctgtcatctc tggttatcag ttttttgttt
     2041 ttgtttttgt ttctttttgt tgttgctgtt gtttttcttg tggtttttct tttctttttt
     2101 tttctctccc tttgttgtct aaggggacaa agaaaaaaaa aaaaaaggac ttgactccat
     2161 gacgtcgtcg tcgactttgg ccactggctg gccggaccgg cgggtgtgag gagttgcaaa
     2221 ccgaaaccaa catgcatttt gggacaattt ctttttaaaa tgtttttacg ccaaaaaaca
     2281 aaagaacaaa caaacaaaca aacccccctt cccccccccc cccattgtgg attcagaacc
     2341 atgtcagatg gaccaggtga atgtatgtgg ggtttgagct tcatggaatc gagtggggaa
     2401 accagctagg gctacactga gggggattag aaggattggt gcaacccaca tacaaacccc
     2461 atgcctgggc accttccagg accgtggaaa gacagggcta gccctcagta gctgcaggga
     2521 ccttcaatgt cattcgtttg taatgaggac tttttaaatg tttttttttt tttaatgggt
     2581 aaacttatag tcaggaaaac taaaagggct tgacgttgaa atgttcaagg ggtactttgc
     2641 aactccgaaa gacattaagc cacagtggtg gcaaaggttt gtccggcgag gagagggagg
     2701 accagacaca tttcttcatg ttagctgctc aaaatgggtt tctgaggtcg tcagctaagc
     2761 taactctgtc tgtaaattgg caaaaaaact tcagggtgac atctgctctt tcatgtatag
     2821 tacatggctt aaggctcgac agatgtctcc aagtcttcaa ctagccagaa ctctggagaa
     2881 gttcagaagt tacataggga ggcttccagg atctgtgccc ctccctacgg ctatgtgaca
     2941 gggacggaga gccaatgaaa cccgtgcctg cgcacacact acttgtgtgt tctagaggct
     3001 ataagctttc tttcctcttc cacacagtca ctgaaacctc agtttcctgt gggccactta
     3061 ggcaaggtcg tcaccctgag aaggcacaaa cgactatcct tagtcgaagc ttcagtgcga
     3121 accacagtga tatcagggtg ccattgtaga gcaaagtgtc aaaacccaga acgttgctca
     3181 gggcactggg cctttgtggg tttttttttt tttttttttt gtagcctgag gcctagagag
     3241 caatcctgaa cctttttaac tttcagccaa cattggaagt ttggttgaag tttgggtggg
     3301 atgacccagg gccccaggag aaaacaggaa gttcaagggg gcttccagac cggtttccag
     3361 ggcttcggaa tgatctggtg atggctgtct tgctagtaat ggggcccaga agactgcctt
     3421 ctggcttgtg cagagtctag gcgttcctgg cttccgtagg gtttcttaag ctcttgattg
     3481 tggtgtagcc actttcccta gccacataag cagttagaat ttgggcaaag actgctgtgt
     3541 ggggagggtc tgtgtgacca aggacttcct gtcactttcc ctttttaaag tctaaggaga
     3601 aacaatccag tggctctacc ccttctggcc aaagatgggg ttcagttagt ttgcaataag
     3661 caccttgtgg ttaaagctac caggtcatac agctcagtcc tagcctatct gcctggaccc
     3721 tggcttggct gagtggcctg ccctagtggc ttatacagac agagcactgc agaattcttc
     3781 ttgtgtttac acaacccttc tgggcaatgc cgttcgtttc tccagaccac ggcacagtcc
     3841 caggatgtct taggattgcc agcaatcaga cagcaccagc tggtcgggca gaaggaactg
     3901 agggatctgc atgggtaact tttttcttaa ctaatcacca aagaaggctg tggaaaggag
     3961 tgaccccacc tctctctccc ccacccttcc gcacgcccca tccagcccag ctagctgccg
     4021 tggggagtat aagaggtcca gccagaacca gagcatgaga gcagaagcat tgtttgtaaa
     4081 agcaacatta gtgaggagag aagctgtcag agtggctttt ctcccccggc agatgggacg
     4141 cccatgagtc cttccatgat tcccgtataa gcaaccttgg cctgcaccta tggcctcgaa
     4201 ggaagcacaa agccctgctc ctctttttct gtctttattg cctcccccat gggtctcaca
     4261 ggctcccgga caccgtacag agcccttctt ctcccttggg gctgcagagg ccacctgcct
     4321 actggttcgg cctctccata ggacagaatt ctcgtccttg gacctttgtg gacttgggag
     4381 aacatctgtt caagtcgacg cccggttagg cctgcagctc tcgcccatct tccagagggc
     4441 acaacattgc caacgaggaa ccactgcctc cgtttgctcc caaggtgcta gggaagatgg
     4501 ttggtttccg tcttctccag ctctgtgggc cgagttccgt gggactttgg actttcatat
     4561 ctggtgacca gtgccctccg cttctgtttg attgcccctt gacctctcta ctatgcactt
     4621 cccttttatc aggtgtataa agttaaatac tatgtattta tcacttacaa gaacatgaac
     4681 ttccaagacg agctttcggt tgtttttttc cacaggtgat cgagcttctc tgtaaacttg
     4741 aaaataaaca gacagcacaa tggtgcgaa
//
