LOCUS       NM_080696               1266 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus calcium voltage-gated channel auxiliary subunit
            gamma 8 (Cacng8), mRNA.
ACCESSION   NM_080696 XM_001066710
VERSION     NM_080696.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Herguedas,B., Kohegyi,B.K., Dohrke,J.N., Watson,J.F., Zhang,D.,
            Ho,H., Shaikh,S.A., Lape,R., Krieger,J.M. and Greger,I.H.
  TITLE     Mechanisms underlying TARP modulation of the GluA1/2-gamma8 AMPA
            receptor
  JOURNAL   Nat Commun 13 (1), 734 (2022)
   PUBMED   35136046
  REMARK    GeneRIF: Mechanisms underlying TARP modulation of the
            GluA1/2-gamma8 AMPA receptor.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Herguedas,B., Watson,J.F., Ho,H., Cais,O., Garcia-Nafria,J. and
            Greger,I.H.
  TITLE     Architecture of the heteromeric GluA1/2 AMPA receptor in complex
            with the auxiliary subunit TARP gamma8
  JOURNAL   Science 364 (6438) (2019)
   PUBMED   30872532
  REMARK    GeneRIF: this study presents a cryo-electron microscopy structure
            of the heteromeric GluA1/2 receptor associated with two
            transmembrane AMPAR regulatory protein (TARP) gamma8 auxiliary
            subunits, the principal AMPAR complex at hippocampal synapses.
REFERENCE   3  (bases 1 to 1266)
  AUTHORS   Matt,L., Kirk,L.M., Chenaux,G., Speca,D.J., Puhger,K.R.,
            Pride,M.C., Qneibi,M., Haham,T., Plambeck,K.E., Stern-Bach,Y.,
            Silverman,J.L., Crawley,J.N., Hell,J.W. and Diaz,E.
  TITLE     SynDIG4/Prrt1 Is Required for Excitatory Synapse Development and
            Plasticity Underlying Cognitive Function
  JOURNAL   Cell Rep 22 (9), 2246-2253 (2018)
   PUBMED   29490264
REFERENCE   4  (bases 1 to 1266)
  AUTHORS   Yamasaki,M., Fukaya,M., Yamazaki,M., Azechi,H., Natsume,R., Abe,M.,
            Sakimura,K. and Watanabe,M.
  TITLE     TARP gamma-2 and gamma-8 Differentially Control AMPAR Density
            Across Schaffer Collateral/Commissural Synapses in the Hippocampal
            CA1 Area
  JOURNAL   J Neurosci 36 (15), 4296-4312 (2016)
   PUBMED   27076426
REFERENCE   5  (bases 1 to 1266)
  AUTHORS   Wang,J.G., Wang,Y.L., Xu,F., Zhao,J.X., Zhou,S.Y., Yu,Y.,
            Chazot,P.L., Wang,X.F. and Lu,C.B.
  TITLE     Activity- and development-dependent down-regulation of TARPgamma8
            and GluA1 in cultured rat hippocampal neurons
  JOURNAL   Acta Pharmacol Sin 37 (3), 303-311 (2016)
   PUBMED   26725511
  REMARK    GeneRIF: AMPA-induced currents were recorded in the neurons using a
            whole-cell recording method. RESULTS: Four-hour exposure to AMPA
            significantly decreased the protein levels of TARP gamma-8 and
            GluA1 in the neurons at 17-18 DIV, but did not change the protein
            level of TARP gamma-8 in the neurons cultured at 7-8 DIV.
REFERENCE   6  (bases 1 to 1266)
  AUTHORS   Milstein,A.D., Zhou,W., Karimzadegan,S., Bredt,D.S. and Nicoll,R.A.
  TITLE     TARP subtypes differentially and dose-dependently control synaptic
            AMPA receptor gating
  JOURNAL   Neuron 55 (6), 905-918 (2007)
   PUBMED   17880894
REFERENCE   7  (bases 1 to 1266)
  AUTHORS   Fukaya,M., Tsujita,M., Yamazaki,M., Kushiya,E., Abe,M., Akashi,K.,
            Natsume,R., Kano,M., Kamiya,H., Watanabe,M. and Sakimura,K.
  TITLE     Abundant distribution of TARP gamma-8 in synaptic and extrasynaptic
            surface of hippocampal neurons and its major role in AMPA receptor
            expression on spines and dendrites
  JOURNAL   Eur J Neurosci 24 (8), 2177-2190 (2006)
   PUBMED   17074043
REFERENCE   8  (bases 1 to 1266)
  AUTHORS   Tomita,S., Fukata,M., Nicoll,R.A. and Bredt,D.S.
  TITLE     Dynamic interaction of stargazin-like TARPs with cycling AMPA
            receptors at synapses
  JOURNAL   Science 303 (5663), 1508-1511 (2004)
   PUBMED   15001777
REFERENCE   9  (bases 1 to 1266)
  AUTHORS   Tomita,S., Chen,L., Kawasaki,Y., Petralia,R.S., Wenthold,R.J.,
            Nicoll,R.A. and Bredt,D.S.
  TITLE     Functional studies and distribution define a family of
            transmembrane AMPA receptor regulatory proteins
  JOURNAL   J Cell Biol 161 (4), 805-816 (2003)
   PUBMED   12771129
REFERENCE   10 (bases 1 to 1266)
  AUTHORS   Chu,P.J., Robertson,H.M. and Best,P.M.
  TITLE     Calcium channel gamma subunits provide insights into the evolution
            of this gene family
  JOURNAL   Gene 280 (1-2), 37-48 (2001)
   PUBMED   11738816
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF361346.1 and AC114434.4.
            
            [WARNING] On Jan 16, 2025 this sequence was replaced by
            NM_080696.3.
            
            On Sep 21, 2007 this sequence version replaced NM_080696.1.
            
            Summary: Voltage-dependent calcium channels couple membrane
            depolarization in a number of cellular processes. These activities
            are regulated by distinct channels composed of the pore-forming
            alpha-1 subunit and the modulatory beta, alpha-2/delta and gamma
            subunits. The protein encoded by this gene represents one of the
            gamma subunits. It is an integral membrane protein that is thought
            to stabilize the calcium channel in an inactive (closed) state.
            This gene is located in a cluster with two similar gamma
            subunit-encoding genes on chromosome 1. The mRNA for this gene is
            believed to initiate translation from a non-AUG (CUG) start codon.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF361346.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: inferred from conservation
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AF361346.1         1-5
            6-7                 AC114434.4         53471-53472
            8-35                AF361346.1         8-35
            36-37               AC114434.4         53501-53502
            38-1266             AF361346.1         38-1266
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q12"
     gene            1..1266
                     /gene="Cacng8"
                     /note="calcium voltage-gated channel auxiliary subunit
                     gamma 8"
                     /db_xref="GeneID:140729"
                     /db_xref="RGD:628808"
     CDS             1..1266
                     /gene="Cacng8"
                     /note="non-AUG (CUG) translation initiation codon;
                     neuronal voltage-gated calcium channel gamma-8 subunit;
                     TARP gamma-8; transmembrane AMPAR regulatory protein
                     gamma-8; calcium channel, voltage-dependent, gamma subunit
                     8"
                     /codon_start=1
                     /product="voltage-dependent calcium channel gamma-8
                     subunit"
                     /protein_id="NP_542427.1"
                     /db_xref="GeneID:140729"
                     /db_xref="RGD:628808"
                     /translation="MESLKRWNEERGLWCEKGVQVLLTTIGAFAAFGLMTIAISTDYW
                     LYTRALICNTTNLTAGDDGPPHRGGSGSSEKKDPGGLTHSGLWRICCLEGLKRGVCVK
                     INHFPEDTDYDHDSAEYLLRVVRASSIFPILSAILLLLGGVCVAASRVYKSKRNIILG
                     AGILFVAAGLSNIIGVIVYISANAGEPGPKRDEEKKNHYSYGWSFYFGGLSFILAEVI
                     GVLAVNIYIERSREAHCQSRSDLLKAGGGAGGSGGSGPSAILRLPSYRFRYRRRSRSS
                     SRGSSEASPSRDASPGGPGGPGFASTDISMYTLSRDPSKGSVAAGLASAGGGGGGAGV
                     GAYGGAAGAAGGGGTGSERDRGSSAGFLTLHNAFPKEAASGVTVTVTGPPAAPAPAPP
                     APAAPAPGTLSKEAAASNTNTLNRKTTPV"
     exon            1..280
                     /gene="Cacng8"
                     /inference="alignment:Splign:2.1.0"
     exon            281..364
                     /gene="Cacng8"
                     /inference="alignment:Splign:2.1.0"
     exon            365..505
                     /gene="Cacng8"
                     /inference="alignment:Splign:2.1.0"
     exon            506..1266
                     /gene="Cacng8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctggaatcat tgaaacgctg gaatgaagag aggggtttgt ggtgcgaaaa gggcgttcag
       61 gtactactga ccaccatagg cgcctttgca gcttttggcc tcatgaccat cgccatcagc
      121 actgactact ggctctacac aagagctctc atctgcaaca ccaccaacct cacagcaggt
      181 gatgatggac caccccatcg tgggggcagt ggctcctcag agaagaagga ccctgggggc
      241 ctcacacatt caggcctctg gcggatatgc tgcctggaag ggttgaaaag aggtgtctgc
      301 gtgaagatca accacttccc ggaggacacg gactacgacc acgacagcgc ggagtacctg
      361 ctccgagtag tccgggcctc cagcatcttt cctatcctga gcgccatcct gctgctgctc
      421 gggggcgtgt gcgtagctgc ctctcgcgtc tacaaatcca aaaggaacat catcctgggc
      481 gcagggatcc tgttcgtggc agcaggcctg agcaacatca tcggggtgat cgtgtacata
      541 tcggcaaacg cgggcgagcc aggccccaag agggacgagg agaagaaaaa ccactattcg
      601 tatggctggt ccttctactt cggcgggctg tcattcatcc tggccgaggt gatcggcgtg
      661 ctagccgtca acatctacat cgagcgcagc cgcgaggcac actgccaatc acgctcggac
      721 ctactcaagg ccggcggcgg cgcgggcggc agtggcggga gcggcccctc ggccatcctc
      781 cgtctgccca gttaccgctt ccgctaccgc cgccgctccc gctccagctc ccgaggctcc
      841 agcgaggcct cgccatcgcg ggatgcgtct cccggcggcc ccgggggccc gggcttcgcc
      901 tccacggaca tctccatgta cacgctcagt cgcgacccgt ccaagggcag cgtggctgcg
      961 gggctggcga gcgccggggg tggaggcggc ggtgccggcg tgggtgccta cggcggggcg
     1021 gccggggcag cggggggcgg cgggacgggc tcggagcggg accgagggag ctcagcgggc
     1081 ttcctcacgc tgcacaacgc cttccccaag gaggcggcgt ccggcgtcac ggtcacggtc
     1141 accggaccgc ccgctgcgcc ggcgcccgcg ccgcccgctc ctgcagcgcc cgcgcccggg
     1201 acgctgtcca aagaggccgc cgcgtccaac accaacacgc tcaacaggaa aaccacgccc
     1261 gtgtag
//
