LOCUS       NM_053873               6125 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus calcium voltage-gated channel subunit alpha1 S
            (Cacna1s), mRNA.
ACCESSION   NM_053873
VERSION     NM_053873.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Obaid,A.A., Almasmoum,H., Almaimani,R.A., El-Boshy,M., Aslam,A.,
            Idris,S., Ghaith,M.M., El-Readi,M.Z., Ahmad,J., Farrash,W.F.,
            Mujalli,A., Eid,S.Y., Elzubier,M.E. and Refaat,B.
  TITLE     Vitamin D and calcium co-therapy mitigates pre-established cadmium
            nephropathy by regulating renal calcium homeostatic molecules and
            improving anti-oxidative and anti-inflammatory activities in rat
  JOURNAL   J Trace Elem Med Biol 79, 127221 (2023)
   PUBMED   37244046
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Wang,M.M., Hao,L.Y., Guo,F., Zhong,B., Zhong,X.M., Yuan,J.,
            Hao,Y.F., Zhao,S., Sun,X.F., Lei,M. and Jiao,G.Y.
  TITLE     Decreased intracellular [Ca2+ ] coincides with reduced expression
            of Dhpralpha1s, RyR1, and diaphragmatic dysfunction in a rat model
            of sepsis
  JOURNAL   Muscle Nerve 56 (6), 1128-1136 (2017)
   PUBMED   28044347
  REMARK    GeneRIF: Septic diaphragm contractility and expression of Cacna1s
            and RyR1 are significantly decreased in a sepsis model compared
            with controls.
REFERENCE   3  (bases 1 to 6125)
  AUTHORS   Tummala,S.R., Neinstein,A., Fina,M.E., Dhingra,A. and Vardi,N.
  TITLE     Localization of Cacna1s to ON bipolar dendritic tips requires
            mGluR6-related cascade elements
  JOURNAL   Invest Ophthalmol Vis Sci 55 (3), 1483-1492 (2014)
   PUBMED   24519419
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6125)
  AUTHORS   Jiao,G.Y., Hao,L.Y., Gao,C.E., Chen,L., Sun,X.F., Yang,H.L., Li,Y.
            and Dai,Y.N.
  TITLE     Reduced DHPRalpha1S and RyR1 expression levels are associated with
            diaphragm contractile dysfunction during sepsis
  JOURNAL   Muscle Nerve 48 (5), 745-751 (2013)
   PUBMED   23943510
  REMARK    GeneRIF: The expression levels of dihydropyridine alpha1s and RyR1
            receptors (the isoforms of skeletal muscles) are significantly
            lower in septic rats than in control rats.
REFERENCE   5  (bases 1 to 6125)
  AUTHORS   Catterall,W.A.
  TITLE     Voltage-gated calcium channels
  JOURNAL   Cold Spring Harb Perspect Biol 3 (8), a003947 (2011)
   PUBMED   21746798
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6125)
  AUTHORS   Chaudhari,N.
  TITLE     A single nucleotide deletion in the skeletal muscle-specific
            calcium channel transcript of muscular dysgenesis (mdg) mice
  JOURNAL   J Biol Chem 267 (36), 25636-25639 (1992)
   PUBMED   1281468
REFERENCE   7  (bases 1 to 6125)
  AUTHORS   Chin,H., Krall,M., Kim,H.L., Kozak,C.A. and Mock,B.
  TITLE     The gene for the alpha 1 subunit of the skeletal muscle
            dihydropyridine-sensitive calcium channel (Cchl1a3) maps to mouse
            chromosome 1
  JOURNAL   Genomics 14 (4), 1089-1091 (1992)
   PUBMED   1335956
REFERENCE   8  (bases 1 to 6125)
  AUTHORS   Yu,A.S., Hebert,S.C., Brenner,B.M. and Lytton,J.
  TITLE     Molecular characterization and nephron distribution of a family of
            transcripts encoding the pore-forming subunit of Ca2+ channels in
            the kidney
  JOURNAL   Proc Natl Acad Sci U S A 89 (21), 10494-10498 (1992)
   PUBMED   1279681
REFERENCE   9  (bases 1 to 6125)
  AUTHORS   Shimahara,T. and Bournaud,R.
  TITLE     Barium currents in developing skeletal muscle cells of normal and
            mutant mice foetuses with 'muscular dysgenesis'
  JOURNAL   Cell Calcium 12 (10), 727-733 (1991)
   PUBMED   1663002
REFERENCE   10 (bases 1 to 6125)
  AUTHORS   Powell,J.A., Friedman,B. and Cossi,A.
  TITLE     Tissue culture study of murine muscular dysgenesis: role of
            spontaneous action potential generation in the regulation of muscle
            maturation
  JOURNAL   Ann N Y Acad Sci 317, 550-570 (1979)
   PUBMED   289331
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB374360.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB374360.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760384, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q13"
     gene            1..6125
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /note="calcium voltage-gated channel subunit alpha1 S"
                     /db_xref="GeneID:682930"
                     /db_xref="RGD:70983"
     exon            1..319
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..5720
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /note="ROB1; voltage-gated calcium channel subunit alpha
                     Cav1.1; calcium channel, voltage-dependent, L type, alpha
                     1S subunit"
                     /codon_start=1
                     /product="voltage-dependent L-type calcium channel subunit
                     alpha-1S"
                     /protein_id="NP_446325.1"
                     /db_xref="GeneID:682930"
                     /db_xref="RGD:70983"
                     /translation="MEPSSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKAC
                     ISVVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAA
                     MKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLD
                     VKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELF
                     KGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFD
                     NFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLS
                     GEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGG
                     SDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIA
                     SEHHNQPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGI
                     LEILLVESGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLL
                     FLFMIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGI
                     MAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERK
                     RRKMSRGLPDKSEEERSTMTKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSAD
                     FPGDDEEDEPEIPASPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNA
                     TWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGA
                     FLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGL
                     KHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYY
                     YIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEED
                     TGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQC
                     VQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQM
                     NHISDILNVAFTIIFTLEMILKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDT
                     LLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIK
                     SFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCA
                     TGEAWQEILLACSYGKRCDPESDYAPGEEYACGTNFAYYYFISFYMLCAFLIINLFVA
                     VIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFG
                     KFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAII
                     KKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDT
                     VQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDN
                     FLERTNSLPPVMANQRPLQFAEMEMEELESPVFLEDFPQNPGTHPLARANTNNANANV
                     AYGNSSHRNSPVFSSIRYERELLEEAGRPVTREGPFSQPCSVSGVNSRSHVDKLERQM
                     SQRRMPKGQVPPSPCQLSQEKHPVHEEGKGPRSWSTETSDSESFEERVPRNSAHKCTA
                     PATTMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKRESPK
                     GGAMPREP"
     exon            320..425
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            426..565
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            566..708
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            709..861
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1067
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1171
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1317
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1399
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1560
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1786
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..1994
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2115
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2230
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2231..2324
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2394
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2527
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2657
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..2717
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2718..2824
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2825..2912
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..3020
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3073
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3074..3220
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..3422
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3423..3581
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3582..3692
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3693..3776
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3777..3833
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3834..3962
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            3963..4028
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4029..4120
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4121..4280
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4281..4408
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4409..4505
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4506..4608
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4609..4710
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4711..4835
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4836..4964
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            4965..5215
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            5216..5301
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            5302..5393
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            5394..5546
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
     exon            5547..6123
                     /gene="Cacna1s"
                     /gene_synonym="Cchl1a3; RGD1565743"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagagagtaa tcctcccacc cccacacccg tccgcccgcc ctgtctgact gcctcagcag
       61 gataggctca gtcaggtcca gtccccaagg tgggggcact ggggacacag ggaggagagg
      121 gcagtggacc gggggagtga gggaagggag gggtgtcagg gaaagccatg gagccgtcct
      181 caccccagga tgagggcctg aggaagaagc agcctaagaa acccgtccct gagattctgc
      241 cgaggccacc ccgggccttg ttctgcttga ctctccagaa ccccctgagg aaggcctgca
      301 tcagcgtcgt ggagtggaaa cccttcgaga ccatcatctt gctcaccatc ttcgccaact
      361 gtgtggccct ggcagtgtac ctgcccatgc cagaggatga caacaacact ctgaaccttg
      421 gcctggagaa actggagtac ttcttcctca tcgtcttctc cattgaggct gctatgaaga
      481 tcatagccta tggcttccta ttccatcagg atgcttacct gcgcagtggc tggaacgtgc
      541 tggacttcat cattgtcttt ctgggtgtct tcaccgcgat tctggaacaa gttaacatca
      601 ttcaaaccaa cacggccccc atgagcagca aaggagccgg cttggatgtg aaggccctgc
      661 gagctttccg cgtgctcaga cccctgcggc tggtgtcagg ggtgcccagc cttcaggtag
      721 tcctcaactc catcttcaag gccatgctcc ccctgttcca catcgccctg ctcgtcctct
      781 tcatggtcat catctatgcc atcatcgggc tggagctctt caagggcaag atgcacaaga
      841 cgtgctactt cattgggaca gacatcgtgg ccacagtaga gaatgagaaa ccatcaccct
      901 gtgctagaac tggctccggg cgcccctgca ccatcaacgg cagcgagtgc cgaggaggtt
      961 ggccaggacc caaccatggc atcacccact tcgacaactt tggcttctct atgctcactg
     1021 tgtaccagtg catctccatg gaggggtgga ccgatgtcct gtactgggtc aatgatgcca
     1081 tcgggaacga gtggccctgg atctactttg tcaccctcat tctgctgggc tccttcttca
     1141 tcctgaacct ggtgctgggt gtcctgagtg gagaattcac caaggagcga gagaaggcca
     1201 agtccagagg aaccttccaa aagctccggg agaagcagca gctggaggaa gaccttcgtg
     1261 gttacatgag ttggatcaca cagggcgagg tcatggacgt ggacgacttg agagaaggca
     1321 agctgtcttt ggatgaaggg ggctcggata cagagagctt atatgaaatc gagggcttga
     1381 acaaaatcat ccaattcatt cggcactggc ggcagtggaa tcgcgtcttc cgctggaagt
     1441 gccatgacct agtgaaatcc aaggtcttct attggctggt gatcctgatt gtggccctca
     1501 acaccctgtc cattgcctca gagcaccaca accagccact ctggctgacg catttgcaag
     1561 atgtcgccaa ccgcgtgctg ctggccctct tcaccatcga gatgctcatg aagatgtacg
     1621 ggctgggcct gcgccagtac ttcatgtcca tcttcaaccg cttcgactgc ttcgtggtgt
     1681 gcagcggcat cctggagatc ttgctggtgg agtcgggagc catgacgccc ctgggcatct
     1741 ctgtgctccg ctgcatccgc ctcctgaggc tcttcaagat caccaagtac tggacatcgc
     1801 tgagcaacct ggtggcctcg ctgctcaact ccatccgctc tatcgcttcc ctcttgctgc
     1861 tgctctttct tttcatgatc atctttgccc tgctaggcat gcagctcttt ggaggcagat
     1921 acgactttga agacacggaa gtacgacgaa gcaactttga caacttcccc caggccctca
     1981 tcagtgtctt ccaggtcctg acgggtgaag actggaactc tgtgatgtac aatgggatca
     2041 tggcctacgg aggcccgtcc taccctggcg tgctcgtgtg catttatttc atcatccttt
     2101 ttgtctgtgg taactacatc cttctcaatg tcttcctggc tatcgctgtg gacaacctgg
     2161 cagaggctga gagcctgaca tctgcccaga aggccaaggc tgaggagaga aagcgcagga
     2221 agatgtctag agggctccca gacaagtcag aagaggagag atccacaatg accaagaagc
     2281 tggagcagaa acccaagggt gaaggcatcc ccaccactgc caagctgaaa atcgatgagt
     2341 ttgaatcgaa cgtcaatgag gtgaaagacc cctacccttc agctgacttc ccaggggacg
     2401 acgaggaaga tgagcctgag atcccagcaa gcccccgacc acgccctctg gctgagctgc
     2461 agctgaaaga gaaagcggtg cccatcccag aggccagctc cttcttcatc ttcagcccca
     2521 ccaataagat ccgcgtccta tgtcaccgca ttgtcaacgc cacctggttc accaacttta
     2581 tcctactctt catcctgctc agtagtgctg ctctggccgc tgaggacccc atccgggccg
     2641 actccatgag gaatcagatc cttgagtatt ttgattatgt gttcactgct gttttcactg
     2701 tggagatcgt ccttaagatg accacctatg gagccttcct gcacaaaggc tccttctgcc
     2761 gcaactactt caacatcctg gacctgctgg tagtggctgt gtctctcatc tccatgggcc
     2821 tcgagtccag cgccatctcc gtggtgaaga tcttgagggt cctgagggtg ttgcgaccac
     2881 tccgggccat aaacagagcc aaagggttga agcacgtggt gcagtgcgtg tttgttgcta
     2941 tccgcaccat cgggaacatc gtgctggtca ccacgctcct gcagttcatg tttgcatgca
     3001 tcggagtcca gcttttcaag ggaaaattct acagctgcaa tgatttatcc aagatgactg
     3061 aagaggagtg caggggctac tactacatat acaaggatgg agaccccacg cagatagagt
     3121 tgcgaccccg tcagtggata cacaatgact tccactttga caatgtcctg tctgctatga
     3181 tgtcactctt cacggtctcc acctttgagg gttggcctca gctactgtac aaggccatag
     3241 actccaatga ggaggacaca ggccccgtct acaacaaccg agtggagatg gccatcttct
     3301 tcatcatcta catcattctc atcgccttct tcatgatgaa catctttgtg ggcttcgtca
     3361 tcgtcacctt ccaggagcag ggggagactg agtataagaa ctgtgagctg gacaagaacc
     3421 agcgccaatg tgtgcagtat gccctgaagg cccgcccgct gaggtgttac atccccaaaa
     3481 acccatacca gtatcaggtg tggtatgtcg tcacttcctc ctactttgaa tacctgatgt
     3541 ttgctctcat catgctcaac actatctgcc taggcatgca gcattacaac cagtcggaac
     3601 agatgaacca catatcggac atcctcaatg tggctttcac catcatcttc accctggaga
     3661 tgatcctcaa gctcatagct ttcaagccca ggggctattt tggagacccc tggaacgtgt
     3721 ttgactttct aatcgtcatt ggcagcatca ttgacgttat cctgagtgag atcgatactt
     3781 tgctggcctc cagcggggga ctatattgcc tgggtggagg ctgcgggaac gttgacccag
     3841 atgagagcgc ccgcatctcc agtgccttct tccgcctgtt ccgagtcatg cgactgatca
     3901 agctgctgag ccgggcggag ggtgtgcgca ccctgctctg gacgttcatc aagtccttcc
     3961 aggccctgcc gtatgtggct ttgctgatcg tcatgctctt cttcatctac gctgtcatcg
     4021 gcatgcagat gttcgggaag atcgccatgg tggacgggac gcaaataaac cggaacaaca
     4081 acttccagac tttcccacaa gctgtgctgc tgctcttcag gtgtgccaca ggggaagcct
     4141 ggcaggagat cctgctggcc tgcagctacg ggaaacgctg cgacccggag tctgactacg
     4201 caccggggga ggagtacgcg tgtggcacca actttgccta ctactacttc atcagcttct
     4261 acatgctctg cgccttcctg atcatcaacc tctttgtggc tgtcatcatg gacaactttg
     4321 actacctcac aagggactgg tccattctgg gccctcacca cctggatgag ttcaaggcca
     4381 tctgggctga gtacgaccca gaggctaagg gcaggatcaa gcacctggac gtggtgaccc
     4441 tgctgaggag gatccagccc cctctgggct ttgggaagtt ctgcccacac agggtagcat
     4501 gtaagaggct ggtgggcatg aacatgcccc tgaatagtga tggcacagtc accttcaatg
     4561 ccacactctt cgccctggta cgcacagccc tcaagatcaa gacagaaggc aattttgaac
     4621 aagccaatga ggagctgaga gccatcatca aaaagatctg gaagagaacc agcatgaagc
     4681 tcctggacca agtcatccct ccaataggag atgacgaggt gacagtgggg aagttctatg
     4741 ccacattcct catccaggag cactttcgga agttcatgaa gcgccaggag gaatattatg
     4801 ggtatcgacc caaaaaggac acagttcaga tacaggctgg attgcggacc atagaggaag
     4861 aggcagctcc tgagatccac cgagccatct ctggagacct gacggcggag gaggagctgg
     4921 agagggccat ggtagaggct gccatggagg aggggatatt ccggagaact ggaggcctgt
     4981 ttggtcaagt ggacaacttc ctggaaagga ccaactctct gccccctgtc atggctaacc
     5041 aaagacccct ccaatttgcc gaaatggaga tggaagagct ggagtcacca gtctttttgg
     5101 aggacttccc tcagaaccca ggcacccacc ctcttgctcg tgccaatacc aacaacgcca
     5161 atgccaatgt tgcctatggc aacagcagcc atagaaacag cccagtgttt tccagcatcc
     5221 gctatgaaag ggagctcctg gaagaggcag gcaggcctgt taccagagaa ggacccttca
     5281 gtcagccctg cagcgtctct ggagttaaca gcagatccca tgtggacaaa ctggaaagac
     5341 agatgtccca aagaagaatg cccaaaggcc aggtgcctcc ttccccatgc cagctctccc
     5401 aggaaaagca tcctgtgcac gaagagggga agggccccag gtcatggtct acggaaacat
     5461 ctgacagtga gagctttgag gagcgtgttc ccaggaattc ggctcacaag tgcacagccc
     5521 cagctaccac catgctgatc caggaggctc tggttcgagg aggactagac tccttagcag
     5581 cagatgctaa cttcgtcatg gcaacaggcc aggccctggc agatgcctgc cagatggaac
     5641 cagaggaagt cgaggtggca gcaacagagc tactgaagcg agaatcccca aagggcgggg
     5701 ccatgcccag ggagccttga gcccaaagtc ctccctgggc agcctgaacc agcagtgggg
     5761 gctcccaaaa agacccttat tgcttccagg tgtgaggcct atacagaatg acacaagctt
     5821 agaactgcca tgaccaaaag tcagaaagcg agggagtgga gaaaggttag cgcagcctag
     5881 cagcctcccc tccctcctca gtagccagct gcatggactg agtagccgtg gtcaggaacc
     5941 acctcaacca ggcagggggc catggatggg caggatcgac ccttctcctt accagcaaga
     6001 agcaggactt ggcagggcct ctgtggtcac tcacaaaggc ctgattggtg ccagggtctg
     6061 tctgtcataa agtcactcca tccaaccagc ttgacattaa agtctccagg cctgactttg
     6121 gcaaa
//
