LOCUS       NM_153814               7871 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus calcium voltage-gated channel subunit alpha1 H
            (Cacna1h), mRNA.
ACCESSION   NM_153814 XM_573058
VERSION     NM_153814.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Nakagawa,M., Takahashi,K., Nishizawa,Y. and Ohta,T.
  TITLE     Involvement of interaction of Cav3.2 and nociceptive TRPA1 in
            pathological pain transmission
  JOURNAL   Biomed Res 45 (1), 45-55 (2024)
   PUBMED   38325845
  REMARK    GeneRIF: Involvement of interaction of Cav3.2 and nociceptive TRPA1
            in pathological pain transmission.
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Casillas-Espinosa,P.M., Lin,R., Li,R., Nandakumar,N.M., Dawson,G.,
            Braine,E.L., Martin,B., Powell,K.L. and O'Brien,T.J.
  TITLE     Effects of the T-type calcium channel CaV3.2 R1584P mutation on
            absence seizure susceptibility in GAERS and NEC congenic rats
            models
  JOURNAL   Neurobiol Dis 184, 106217 (2023)
   PUBMED   37391087
  REMARK    GeneRIF: Effects of the T-type calcium channel CaV3.2 R1584P
            mutation on absence seizure susceptibility in GAERS and NEC
            congenic rats models.
REFERENCE   3  (bases 1 to 7871)
  AUTHORS   Tomita,S., Sekiguchi,F., Kasanami,Y., Naoe,K., Tsubota,M., Wake,H.,
            Nishibori,M. and Kawabata,A.
  TITLE     Cav3.2 overexpression in L4 dorsal root ganglion neurons after L5
            spinal nerve cutting involves Egr-1, USP5 and HMGB1 in rats: An
            emerging signaling pathway for neuropathic pain
  JOURNAL   Eur J Pharmacol 888, 173587 (2020)
   PUBMED   32971090
  REMARK    GeneRIF: Cav3.2 overexpression in L4 dorsal root ganglion neurons
            after L5 spinal nerve cutting involves Egr-1, USP5 and HMGB1 in
            rats: An emerging signaling pathway for neuropathic pain.
REFERENCE   4  (bases 1 to 7871)
  AUTHORS   Gomez,K., Calderon-Rivera,A., Sandoval,A., Gonzalez-Ramirez,R.,
            Vargas-Parada,A., Ojeda-Alonso,J., Granados-Soto,V.,
            Delgado-Lezama,R. and Felix,R.
  TITLE     Cdk5-Dependent Phosphorylation of CaV3.2 T-Type Channels: Possible
            Role in Nerve Ligation-Induced Neuropathic Allodynia and the
            Compound Action Potential in Primary Afferent C Fibers
  JOURNAL   J Neurosci 40 (2), 283-296 (2020)
   PUBMED   31744861
  REMARK    GeneRIF: The present work shows that the exacerbated expression of
            Cdk5 in a preclinical model of neuropathic pain increases the
            functional expression of CaV3.2 channels
REFERENCE   5  (bases 1 to 7871)
  AUTHORS   Liu,Q.Y., Chen,W., Cui,S., Liao,F.F., Yi,M., Liu,F.Y. and Wan,Y.
  TITLE     Upregulation of Cav3.2 T-type calcium channels in adjacent intact
            L4 dorsal root ganglion neurons in neuropathic pain rats with L5
            spinal nerve ligation
  JOURNAL   Neurosci Res 142, 30-37 (2019)
   PUBMED   29684385
  REMARK    GeneRIF: Study shows that the membrane expression and the function
            of Cav3.2 T-type calcium channels are increased in the intact L4
            dorsal root ganglion neurons in neuropathic pain rats with
            peripheral nerve injury like spinal nerve ligation.
REFERENCE   6  (bases 1 to 7871)
  AUTHORS   Bourinet,E., Alloui,A., Monteil,A., Barrere,C., Couette,B.,
            Poirot,O., Pages,A., McRory,J., Snutch,T.P., Eschalier,A. and
            Nargeot,J.
  TITLE     Silencing of the Cav3.2 T-type calcium channel gene in sensory
            neurons demonstrates its major role in nociception
  JOURNAL   EMBO J 24 (2), 315-324 (2005)
   PUBMED   15616581
  REMARK    GeneRIF: results provide direct evidence linking CaV3.2 T-type
            channels to pain perception and suggest that CaV3.2 may offer a
            specific molecular target for the treatment of pain.
REFERENCE   7  (bases 1 to 7871)
  AUTHORS   Khosravani,H., Altier,C., Simms,B., Hamming,K.S., Snutch,T.P.,
            Mezeyova,J., McRory,J.E. and Zamponi,G.W.
  TITLE     Gating effects of mutations in the Cav3.2 T-type calcium channel
            associated with childhood absence epilepsy
  JOURNAL   J Biol Chem 279 (11), 9681-9684 (2004)
   PUBMED   14729682
  REMARK    GeneRIF: Ca(v)3.2 has a role in calcium influx during physiological
            activation and mutations may be causative in the propensity for
            seizures in patients with childhood absence epilepsy
REFERENCE   8  (bases 1 to 7871)
  AUTHORS   Chen,Y., Lu,J., Pan,H., Zhang,Y., Wu,H., Xu,K., Liu,X., Jiang,Y.,
            Bao,X., Yao,Z., Ding,K., Lo,W.H., Qiang,B., Chan,P., Shen,Y. and
            Wu,X.
  TITLE     Association between genetic variation of CACNA1H and childhood
            absence epilepsy
  JOURNAL   Ann Neurol 54 (2), 239-243 (2003)
   PUBMED   12891677
REFERENCE   9  (bases 1 to 7871)
  AUTHORS   Lenglet,S., Louiset,E., Delarue,C., Vaudry,H. and Contesse,V.
  TITLE     Involvement of T-type calcium channels in the mechanism of action
            of 5-HT in rat glomerulosa cells: a novel signaling pathway for the
            5-HT7 receptor
  JOURNAL   Endocr Res 28 (4), 651-655 (2002)
   PUBMED   12530678
  REMARK    GeneRIF: Activation of native 5-HT7 receptors stimulates cAMP
            formation, which in turn provokes calcium influx through T-type
            calcium channels. Both activation of AC/PKA pathway and calcium
            influx are involved in 5-HT-induced aldosterone secretion.
REFERENCE   10 (bases 1 to 7871)
  AUTHORS   McRory,J.E., Santi,C.M., Hamming,K.S., Mezeyova,J., Sutton,K.G.,
            Baillie,D.L., Stea,A. and Snutch,T.P.
  TITLE     Molecular and functional characterization of a family of rat brain
            T-type calcium channels
  JOURNAL   J Biol Chem 276 (6), 3999-4011 (2001)
   PUBMED   11073957
  REMARK    Erratum:[J Biol Chem 2001 Aug 10;276(32):30571-4]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF290213.2.
            
            On Aug 31, 2012 this sequence version replaced NM_153814.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF290213.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132296,
                                           SAMD00132300 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7871              AF290213.2         4-7874
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q12"
     gene            1..7871
                     /gene="Cacna1h"
                     /note="calcium voltage-gated channel subunit alpha1 H"
                     /db_xref="GeneID:114862"
                     /db_xref="RGD:68943"
     exon            1..326
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..7107
                     /gene="Cacna1h"
                     /note="voltage-gated calcium channel subunit alpha Cav3.2;
                     calcium channel, voltage-dependent, T type, alpha 1H
                     subunit"
                     /codon_start=1
                     /product="voltage-dependent T-type calcium channel subunit
                     alpha-1H"
                     /protein_id="NP_722521.1"
                     /db_xref="GeneID:114862"
                     /db_xref="RGD:68943"
                     /translation="MTEGTLAADEVRVPLGASPPAPAAPVRASPASPGAPGREEQGGS
                     GSGVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWF
                     EHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGL
                     FGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRI
                     LVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLR
                     PYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGR
                     NACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHS
                     FYNFIYFILLIIMGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASF
                     SEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTVHGQGPGRRPRRA
                     GRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDNRLVRACAPPSPPSPGHG
                     PPDSESVHSIYHADCHVEGPQERARVAHSIATAASLKLASGLGTMNYPTILPSGTVNS
                     KGGTSSRPKGLRGAGAPGAAVHSPLSLGSPRPYEKIQDVVGEQGLGRASSHLSGLSVP
                     CPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCR
                     DPVQQPHEVGTPGHSNERRRTPLRKASQPGGIGHLWASFSGKLRRIVDSKYFNRGIMA
                     AILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNI
                     FDGIVVVISVWEIVGQANGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVAT
                     FCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQED
                     WNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDK
                     TSTQLEGDFDKLRDLRATEMKMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSS
                     PNLDVAHALLDSRRSSSGSVDPQLGDQKSLASLRSSPCTPWGPNSAGSSRRSSWNSLG
                     RAPSLKRRNQCGERESLLSGEGKGSTDDEAEDSRPSTGTHPGASPGPRATPLRRAESL
                     DHRSTLDLCPPRPAALLPTKFHDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDSCC
                     FRLHKVLEPYAPQWCRSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCI
                     TIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVL
                     DGLLVLVSLVDIIVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLIS
                     SLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRR
                     KYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLI
                     VSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSH
                     TRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFE
                     AALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLR
                     IARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSED
                     NPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSP
                     VYFVTFMLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEMAQGSTAQPPPT
                     AQESQGTQPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYT
                     GPVTSAHSPPLEPRASFQVPSAASSPARVSDPLCALSPRGTPRSLSLSRILCRQEAMH
                     SESLEGKVDDVGGDSIPDYTEPAENMSTSQASTGAPRSPPCSPRPASVRTRKHTFGQR
                     CISSRPPTLGGDEAEAADPADEEVSHITSSAHPWPATEPHSPEASPTASPVKGTMGSG
                     RDPRRFCSVDAQSFLDKPGRPDAQRWSSVELDNGESHLESGEVRGRASELEPALGSRR
                     KKKMSPPCISIEPPTKDEGSSRPPAAEGGNTTLRRRTPSCEAALHRDCPEPTEGPGTG
                     GDPVAKGERWGQASCRAEHLTVPNFAFEPLDMGGPGGDCFLDSDQSVTPEPRVSSLGA
                     IVPLILETELSMPSGDCPEKEQGLYLTVPQTPLKKPGSTPATPAPDDSGDEPV"
     exon            327..438
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            439..572
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            573..670
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            671..830
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            831..1146
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1239
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..2029
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2469
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            2470..2621
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2807
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2925
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..3081
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..3172
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3381
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            3382..3804
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            3805..3905
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            3906..4029
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            4030..4098
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            4099..4283
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            4284..4410
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            4411..4536
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            4537..4626
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            4627..4819
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            4820..4971
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            4972..5081
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            5082..5215
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            5216..5286
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            5287..5365
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            5366..5487
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            5488..5733
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            5734..5902
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            5903..6063
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
     exon            6064..7871
                     /gene="Cacna1h"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tctccgcagg tgctgccctc cgccaccatg accgagggca cgctggcagc ggacgaagtc
       61 cgggtgcccc tgggcgcttc gccgccggcc cctgcagcgc cggtgagagc ttccccagcg
      121 agccctgggg cgccggggcg cgaggagcag ggaggatccg ggtcgggcgt gttggctccc
      181 gagagcccag ggaccgagtg tggtgcggac ctgggcgccg acgaggaaca gccggtccca
      241 tacccagctc tggctgccac agtcttcttc tgcctcgggc aaaccacgcg gccgcgcagc
      301 tggtgcctcc gactggtttg taacccgtgg ttcgagcaca tcagcatgct ggtcatcatg
      361 ctgaactgcg tgacactggg catgttcagg ccctgtgagg atgttgagtg ccgctccgaa
      421 cgttgcagca tcttggaggc cttcgacgac ttcatctttg ccttcttcgc cgtggagatg
      481 gtgatcaaga tggtggcttt ggggctgttt gggcaaaaat gctacctggg tgacacctgg
      541 aacaggctgg acttcttcat tgtcatggcg ggcatgatgg agtactctct ggacggacac
      601 aacgtgagcc tctctgccat ccgaaccgtg cgtgtgctgc ggcccctccg cgccatcaac
      661 cgagtcccca gtatgcggat cctggtcact ctgctgctgg acacgctgcc catgcttggg
      721 aatgtcctcc tcctctgctt cttcgtcttc ttcatcttcg gcattgttgg ggtccagctc
      781 tgggctggcc tgcttcggaa ccgatgcttc ctggacagcg ccttcgtcag gaacaacaac
      841 ctgaccttct tgcggccata ctaccagacg gaggagggtg aggagaaccc tttcatctgc
      901 tcctcccgcc gtgacaacgg catgcagaag tgctcgcaca tccccagccg ccgtgagctt
      961 cgagtgcagt gcacactcgg ctgggaggcc tatgggcagc cacaggctga ggatgggggt
     1021 gctggccgca acgcctgtat caactggaac cagtattaca acgtgtgccg ctcgggggaa
     1081 ttcaaccctc acaacggtgc catcaacttc gacaacattg gctacgcttg gattgccatc
     1141 ttccaggtca tcacactgga gggctgggtg gacatcatgt actacgtcat ggatgcccac
     1201 tcgttctaca acttcatcta cttcatcctc ctcatcatta tgggctcctt cttcatgatc
     1261 aacctgtgcc tggtggtgat agccacacag ttctcagaga caaagcaaag ggaaaaccag
     1321 ctgatgcgag aacagcgggc ccgctatctg tccaacgaca gcactctggc cagcttctca
     1381 gagcccggca gctgctacga ggagctcctc aagtatgtag gccacatctt ccggaaggtt
     1441 aaacgccgta gcctgcgtct ttatgcccgc tggcagagcc gctggcgtaa gaaggtggat
     1501 cccagcagta ccgtgcatgg ccaaggccct gggcggcggc cacgacgggc aggcaggcgt
     1561 acagcttcag tgcaccatct ggtctaccac caccaccacc accatcacca ccattaccac
     1621 tttagccacg gtggcccacg caggcccagc ccagagccag gtgctggtga caacaggttg
     1681 gtcagggcct gtgcgccacc ctcgccgcca tccccaggcc atgggccacc agactctgag
     1741 tctgtgcaca gtatctacca tgctgactgc cacgtggagg ggccgcagga acgagcccga
     1801 gtggcacact ccatagccac tgctgctagc ctcaagctgg cctcaggttt gggtaccatg
     1861 aactacccca ccatcctacc ttcaggaaca gtcaacagca aaggtggcac cagctcacga
     1921 cccaaggggc tacgaggtgc tggcgcccca ggggctgcag tacacagccc tctgagcctg
     1981 ggaagcccca gaccctatga gaagatccag gatgtggtgg gagaacaagg actaggccga
     2041 gcctctagcc acctgtcagg cctgagtgtg ccttgccccc tgcccagccc ccaggctggc
     2101 acgctgacct gtgagctgaa gagctgccca tattgtgcca gcgccctgga ggaccccgag
     2161 tttgaattca gtggctcaga gagcggggac tcggatgccc acggagtcta tgagtttacc
     2221 caggatgtac ggcatgggga ttgtcgggac cctgtgcagc agccccatga agtgggcaca
     2281 ccaggccaca gcaatgagcg gcggcggaca ccactgcgga aggcctcaca accaggaggg
     2341 ataggccacc tctgggcatc cttcagtggc aagctacgtc gcattgtaga cagcaagtac
     2401 ttcaaccgag gcatcatggc agccatcctc gtcaatactc tgagcatggg cgttgagtat
     2461 catgaacagc ctgaggagct gaccaacgcc ctggagataa gcaacatcgt gttcaccagc
     2521 atgtttgcct tggagatgct actgaagctg ctggcctgcg gcccactggg atacatccgg
     2581 aacccctaca acatcttcga tggcattgtt gtcgtcataa gtgtctggga gatcgtgggg
     2641 caggcaaacg gtggcctgtc tgtgctgcgc accttcaggc tgctgcgggt gctgaagctg
     2701 gtgcgcttcc tgccggccct gcggcgccag ctcgtggtgc tcatgaggac catggacaac
     2761 gtggccacct tctgcatgct cctcatgctg ttcatcttca tcttcagcat cctgggcatg
     2821 cacctgttcg gctgtaagtt cagcctgaag acagactctg gagacaccgt ccctgacagg
     2881 aagaacttcg actccctact gtgggccatc gtcaccgtgt ttcagatctt gacacaggaa
     2941 gactggaacg tggttctgta caacggcatg gcctccactt cgtcctgggc cgccctttac
     3001 tttgtggccc tcatgacctt tgggaactat gtgctcttca acctgctggt agccatcctg
     3061 gtggaaggtt tccaggcaga gggtgacgcc accagatctg acaccgacga ggataagacg
     3121 tctacccagc tagagggaga tttcgataag ctcagagatc ttcgagccac agagatgaag
     3181 atgtattcac tggcagtgac ccctaacggg cacctagagg gccgaggcag cctgccgccg
     3241 cccctcatca ctcacacggc agctacgcct atgcctactc ccaaaagctc cccaaacctg
     3301 gacgtggccc atgctctcct ggactctcgg cgcagcagca gcggctctgt ggacccccag
     3361 ctgggggacc agaagtctct ggccagcctc cgcagctccc cttgcacccc atggggcccc
     3421 aacagcgctg ggagcagcag gcgctccagt tggaacagcc tgggccgcgc acccagcctc
     3481 aaacgccgca accagtgtgg ggagcgcgag tccctgctct ctggagaggg gaagggcagc
     3541 accgatgacg aggccgagga cagcagacca agcacgggaa cccacccagg ggcctcgcca
     3601 gggccccgag ccacgccact gcggcgtgcc gagtcattgg accaccgcag cacgctggac
     3661 ctgtgtccac cacggcctgc ggccctcctg ccgaccaagt tccatgactg caacgggcag
     3721 atggtggccc tgcccagcga gttctttctg cgcatcgaca gccacaagga ggatgcagcg
     3781 gagtttgatg atgacataga ggatagctgc tgcttccgtc tacacaaagt gctggaaccc
     3841 tatgcacccc agtggtgccg tagccgggag tcctgggccc tgtatctctt cccaccgcag
     3901 aacaggctac gcgtctcctg ccagaaagtc atcgcacaca agatgtttga ccacgtggtc
     3961 cttgtcttca tcttcctcaa ctgtatcacc attgctctgg agaggccaga cattgaccca
     4021 ggcagcactg agcgggcctt cctcagcgtc tccaactaca tcttcacagc catcttcgtg
     4081 gtggagatga tggtgaaggt ggtagccctg ggactgctgt ggggtgaaca tgcctaccta
     4141 cagagcagtt ggaatgtgct ggacgggctg cttgtcctgg tatccctggt tgacatcatc
     4201 gtggccatgg cctcagctgg cggtgccaag atcctaggcg tcctgcgtgt gctgcgcctg
     4261 ctgcggaccc tgaggcctct gagggtcatc agccgagctc caggcctcaa gctggttgta
     4321 gagactctga tatcatcgct caggcccatt gggaacatcg tcctcatctg ctgcgccttc
     4381 ttcatcatct ttggcatcct cggggtgcag cttttcaagg gcaaattcta ctactgcgag
     4441 ggcacagata ccaggaatat caccaccaag gccgagtgcc atgctgccca ctaccgctgg
     4501 gtgaggcgca aatacaactt tgacaacctg ggtcaggcgc tgatgtctct gttcgtgctg
     4561 tcatctaagg atggctgggt aaacatcatg tatgacgggc tggatgccgt gggcatcgac
     4621 cagcagcccg tgcagaacca caacccctgg atgctgctct acttcatctc cttcctgctc
     4681 atcgtcagct tcttcgtgct caacatgttt gtgggcgtgg tggtggagaa cttccacaag
     4741 tgccggcagc accaggaggc tgaggaggct cggcgccggg aggagaaacg gctgcggcgc
     4801 ctggagagga ggcgcaggaa ggcccagcgc cggccctact acgcagacta ttcacacact
     4861 cgccgctcca tccattcgct gtgcaccagc cactacctgg acctcttcat caccttcatc
     4921 atctgcctca atgtcatcac catgtccatg gagcactaca accagcccaa gtccctggat
     4981 gaggccctca agtactgcaa ctacgtcttt accatcgtct tcgtctttga ggctgcactg
     5041 aagctggtgg cctttgggtt ccggaggttc ttcaaggaca ggtggaacca gctggacttg
     5101 gccatcgtcc tcctatccat catgggcatt gcgctggagg agattgagat gaacgccgcc
     5161 ctgcccatca atcccaccat catccgcatc atgcgtgtgc ttcgaatcgc ccgtgtgctg
     5221 aagctactga agatggccac aggcatgcgc gccttgctgg atactgtggt tcaagctctg
     5281 cctcaggtag ggaaccttgg tcttcttttc atgctcctgt tttttatcta tgctgccctg
     5341 ggagtggagc tgtttgggag gctagagtgc agcgaggata acccctgcga gggcctgagc
     5401 aggcacgcta ccttcaccaa cttcggcatg gccttcctca cactgttccg agtgtccact
     5461 ggggacaact ggaatgggat tatgaaggat accctccgtg agtgtacccg tgaggacaag
     5521 cactgcctca gctacctgcc cgcgctctca cccgtctact tcgtcacctt catgctggtg
     5581 gctcagttcg tgctggtcaa tgtggtggtg gccgtgctca tgaagcacct ggaggagagc
     5641 aacaaggagg cccgcgagga tgcagagatg gacgccgaga tcgagctgga gatggcacag
     5701 gggtccacag cccagccccc acctacagca caggaaagcc aaggtaccca gccagacacc
     5761 ccgaacctcc tggtcgtgcg aaaagtatct gtgtccagga tgctctcgct gcccaatgac
     5821 agctacatgt tcaggccggt ggctcccgcg gctgccccac attcccaccc actgcaggaa
     5881 gtggagatgg agacctacac aggcccggtc acctctgctc actcgccacc cctggagccc
     5941 cgcgcctctt tccaggtccc atcagccgcg tcctccccag ccagggtcag tgaccccctt
     6001 tgtgcccttt caccccgggg tacaccccgc tctctgagtc tctcacggat actctgcaga
     6061 caggaggcca tgcactctga gtccctggaa gggaaggttg atgatgttgg aggagacagc
     6121 atcccagact acacagagcc tgctgaaaat atgtccacga gccaggcatc aacaggtgcc
     6181 ccgaggtccc ctccgtgctc cccgcgacct gccagcgtcc gtacccgcaa gcacacgttt
     6241 gggcaacgct gcatctccag ccgccctccc accctgggag gagatgaggc tgaagcagca
     6301 gacccagcag atgaggaggt cagccacatc accagctcag cccacccctg gccggctaca
     6361 gagccccaca gccctgaggc ctccccaaca gcctctcctg tgaaaggcac aatgggcagt
     6421 gggcgggacc cacgcaggtt ctgcagtgta gatgctcaga gcttcctgga caaaccaggt
     6481 cggccagatg cacaacggtg gtcctcagtg gaactggata acggagaaag ccacctagag
     6541 tccggggaag tgaggggccg ggcctcagag ctcgaaccag ctcttgggtc acgaaggaag
     6601 aagaagatga gccctccctg catctccatt gaacctccca ctaaggatga gggctcttcc
     6661 cggccccctg cagccgaagg aggcaacact accctgaggc gccgaacccc atcctgtgag
     6721 gctgccctcc atagggactg cccagagcct acagaaggcc caggcaccgg aggggaccct
     6781 gtagccaagg gtgagcgctg gggccaggcc tcttgccgag cagagcatct gactgtcccc
     6841 aactttgcct ttgagcctct ggacatgggc ggacctggtg gagactgttt cttggacagt
     6901 gaccaaagtg tgaccccaga acccagagtt tcctctttgg gggctatagt gcctctgata
     6961 ctagaaactg aactttctat gccctctggt gactgcccag agaaggaaca aggactgtac
     7021 ctcactgtgc cccagacccc cttgaagaaa ccagggtcta ccccagccac tcctgcccca
     7081 gatgacagtg gagatgagcc tgtgtagatg gggctgcgtg tccacagggc tttggcattg
     7141 aggttgttgg ctccctgcag ggtggtaggg ccatgagtgg accctggctt aggccccact
     7201 aaggcagagg gaccgggaga taaccatccc aggagaggca gcagacatcc cgtctctgca
     7261 ccatgacaca ggagcagcct cgggccccac gagcctccct cgtggtgatt caggtttggg
     7321 ttttcctgag ttttaaccac caccagaagc tgtaccagga ccaggtcatc agtctcagga
     7381 ggagaggctg tgtcctggga aggaccagta attcctcaca ggcaggaggg caccacagct
     7441 ccatccatgt gacacacagg tttccgacag ggagtacagg cttgagcctg tgtacattgg
     7501 gtcctgcagc caccgcaccc aatatcacct tcgttcacag tcctgtttct gtccaccact
     7561 cggcatcctt ccctctcaca gtgcccctcc cccattccat ccccttagat ggtctagaac
     7621 tttgcagtga ccctgggaag tactgaccca tgcaataaga cattgcagtc ccaactgagg
     7681 tggggcttcc catccattcc aggctgttgg gctcaacatt catttgacat ccatttgctt
     7741 tatgtcatcc gtttctacaa attcaggtta aatgttgcaa taatctgatg cagaaaactt
     7801 ggcttcctaa gtcaaagctg aggggagggg aggggagggg caaggcaaat ctgaataaac
     7861 actaacttat t
//
