LOCUS       NM_001195199            9783 bp    mRNA    linear   ROD 07-DEC-2024
DEFINITION  Rattus norvegicus calcium voltage-gated channel subunit alpha1 B
            (Cacna1b), transcript variant 1, mRNA.
ACCESSION   NM_001195199
VERSION     NM_001195199.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Cai,Z., Quan,L., Chang,X., Qiu,Z. and Zhou,H.
  TITLE     High-voltage long-duration pulsed radiofrequency attenuates
            neuropathic pain in CCI rats by inhibiting Cav2.2 in spinal dorsal
            horn and dorsal root ganglion
  JOURNAL   Brain Res 1785, 147892 (2022)
   PUBMED   35341732
  REMARK    GeneRIF: High-voltage long-duration pulsed radiofrequency
            attenuates neuropathic pain in CCI rats by inhibiting Cav2.2 in
            spinal dorsal horn and dorsal root ganglion.
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Silveirinha,V.C., Lin,H., Tanifuji,S., Mochida,S., Cottrell,G.S.,
            Cimarosti,H. and Stephens,G.J.
  TITLE     CaV2.2 (N-type) voltage-gated calcium channels are activated by
            SUMOylation pathways
  JOURNAL   Cell Calcium 93, 102326 (2021)
   PUBMED   33360835
  REMARK    GeneRIF: CaV2.2 (N-type) voltage-gated calcium channels are
            activated by SUMOylation pathways.
REFERENCE   3  (bases 1 to 9783)
  AUTHORS   Castro,H., Bermeo,K., Arenas,I. and Garcia,D.E.
  TITLE     Maintenance of CaV2.2 channel-current by PIP2 unveiled by neomycin
            in sympathetic neurons of the rat
  JOURNAL   Arch Biochem Biophys 682, 108261 (2020)
   PUBMED   31923392
  REMARK    GeneRIF: interacting PIP2 charges not only underly the maintenance
            of the channel-current but also that charge screening of PIP2 by
            itself unveils the voltage-independent features of CaV2.2 channels
            in SCG neurons
REFERENCE   4  (bases 1 to 9783)
  AUTHORS   Buchta,W.C., Moutal,A., Hines,B., Garcia-Keller,C., Smith,A.C.W.,
            Kalivas,P., Khanna,R. and Riegel,A.C.
  TITLE     Dynamic CRMP2 Regulation of CaV2.2 in the Prefrontal Cortex
            Contributes to the Reinstatement of Cocaine Seeking
  JOURNAL   Mol Neurobiol 57 (1), 346-357 (2020)
   PUBMED   31359322
  REMARK    GeneRIF: Dynamic CRMP2 Regulation of CaV2.2 in the Prefrontal
            Cortex Contributes to the Reinstatement of Cocaine Seeking.
REFERENCE   5  (bases 1 to 9783)
  AUTHORS   Gandini,M.A., Souza,I.A., Raval,D., Xu,J., Pan,Y.X. and
            Zamponi,G.W.
  TITLE     Differential regulation of Cav2.2 channel exon 37 variants by
            alternatively spliced mu-opioid receptors
  JOURNAL   Mol Brain 12 (1), 98 (2019)
   PUBMED   31775826
  REMARK    GeneRIF: This study provides new insights into the roles of the MOR
            full-length C-terminal variants in modulating Cav2.2 channel
            isoform activities.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9783)
  AUTHORS   Wheeler,D.B., Randall,A. and Tsien,R.W.
  TITLE     Roles of N-type and Q-type Ca2+ channels in supporting hippocampal
            synaptic transmission
  JOURNAL   Science 264 (5155), 107-111 (1994)
   PUBMED   7832825
REFERENCE   7  (bases 1 to 9783)
  AUTHORS   Hell,J.W., Appleyard,S.M., Yokoyama,C.T., Warner,C. and
            Catterall,W.A.
  TITLE     Differential phosphorylation of two size forms of the N-type
            calcium channel alpha 1 subunit which have different COOH termini
  JOURNAL   J Biol Chem 269 (10), 7390-7396 (1994)
   PUBMED   8125957
REFERENCE   8  (bases 1 to 9783)
  AUTHORS   Pragnell,M., De Waard,M., Mori,Y., Tanabe,T., Snutch,T.P. and
            Campbell,K.P.
  TITLE     Calcium channel beta-subunit binds to a conserved motif in the I-II
            cytoplasmic linker of the alpha 1-subunit
  JOURNAL   Nature 368 (6466), 67-70 (1994)
   PUBMED   7509046
REFERENCE   9  (bases 1 to 9783)
  AUTHORS   Dubel,S.J., Starr,T.V., Hell,J., Ahlijanian,M.K., Enyeart,J.J.,
            Catterall,W.A. and Snutch,T.P.
  TITLE     Molecular cloning of the alpha-1 subunit of an
            omega-conotoxin-sensitive calcium channel
  JOURNAL   Proc Natl Acad Sci U S A 89 (11), 5058-5062 (1992)
   PUBMED   1317580
REFERENCE   10 (bases 1 to 9783)
  AUTHORS   Snutch,T.P., Leonard,J.P., Gilbert,M.M., Lester,H.A. and
            Davidson,N.
  TITLE     Rat brain expresses a heterogeneous family of calcium channels
  JOURNAL   Proc Natl Acad Sci U S A 87 (9), 3391-3395 (1990)
   PUBMED   1692134
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Oct 27, 2022 this sequence version replaced NM_001195199.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HQ008360.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-408               JAXUCZ010000003.1  27943878-27944285   c
            409-514             JAXUCZ010000003.1  27942891-27942996   c
            515-654             JAXUCZ010000003.1  27939349-27939488   c
            655-746             JAXUCZ010000003.1  27916401-27916492   c
            747-899             JAXUCZ010000003.1  27914540-27914692   c
            900-1090            JAXUCZ010000003.1  27913068-27913258   c
            1091-1194           JAXUCZ010000003.1  27902794-27902897   c
            1195-1310           JAXUCZ010000003.1  27901507-27901622   c
            1311-1367           JAXUCZ010000003.1  27900769-27900825   c
            1368-1460           JAXUCZ010000003.1  27899990-27900082   c
            1461-1670           JAXUCZ010000003.1  27887072-27887281   c
            1671-1783           JAXUCZ010000003.1  27884057-27884169   c
            1784-1896           JAXUCZ010000003.1  27881097-27881209   c
            1897-2028           JAXUCZ010000003.1  27878361-27878492   c
            2029-2101           JAXUCZ010000003.1  27878080-27878152   c
            2102-2219           JAXUCZ010000003.1  27871616-27871733   c
            2220-2287           JAXUCZ010000003.1  27869973-27870040   c
            2288-2394           JAXUCZ010000003.1  27867001-27867107   c
            2395-2457           JAXUCZ010000003.1  27864165-27864227   c
            2458-3246           JAXUCZ010000003.1  27860079-27860867   c
            3247-3464           JAXUCZ010000003.1  27859039-27859256   c
            3465-3591           JAXUCZ010000003.1  27837700-27837826   c
            3592-3721           JAXUCZ010000003.1  27836418-27836547   c
            3722-3781           JAXUCZ010000003.1  27836050-27836109   c
            3782-3888           JAXUCZ010000003.1  27833925-27834031   c
            3889-3985           JAXUCZ010000003.1  27831213-27831309   c
            3986-4146           JAXUCZ010000003.1  27829802-27829962   c
            4147-4284           JAXUCZ010000003.1  27828257-27828394   c
            4285-4486           JAXUCZ010000003.1  27827624-27827825   c
            4487-4651           JAXUCZ010000003.1  27827206-27827370   c
            4652-4762           JAXUCZ010000003.1  27826283-27826393   c
            4763-4846           JAXUCZ010000003.1  27825837-27825920   c
            4847-4852           JAXUCZ010000003.1  27818583-27818588   c
            4853-4969           JAXUCZ010000003.1  27814775-27814891   c
            4970-5035           JAXUCZ010000003.1  27814348-27814413   c
            5036-5127           JAXUCZ010000003.1  27813116-27813207   c
            5128-5272           JAXUCZ010000003.1  27811219-27811363   c
            5273-5400           JAXUCZ010000003.1  27808614-27808741   c
            5401-5497           JAXUCZ010000003.1  27801147-27801243   c
            5498-5606           JAXUCZ010000003.1  27798481-27798589   c
            5607-5714           JAXUCZ010000003.1  27794321-27794428   c
            5715-5827           JAXUCZ010000003.1  27793727-27793839   c
            5828-5955           JAXUCZ010000003.1  27792869-27792996   c
            5956-6091           JAXUCZ010000003.1  27786126-27786261   c
            6092-6208           JAXUCZ010000003.1  27785859-27785975   c
            6209-6413           JAXUCZ010000003.1  27783218-27783422   c
            6414-6658           JAXUCZ010000003.1  27782741-27782985   c
            6659-9783           JAXUCZ010000003.1  27779139-27782263   c
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p13"
     gene            1..9783
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /note="calcium voltage-gated channel subunit alpha1 B"
                     /db_xref="GeneID:257648"
                     /db_xref="RGD:628852"
     exon            1..408
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..7189
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /note="isoform 1 is encoded by transcript variant 1;
                     voltage-dependent N-type calcium channel subunit alpha-1B;
                     voltage-dependent N-type calcium channel subunit
                     alpha-1B-like; voltage gated N-type calcium channel
                     Ca(v)2.2; brain calcium channel III; voltage-gated calcium
                     channel subunit alpha Cav2.2; calcium channel, L type,
                     alpha-1 polypeptide; calcium channel, voltage-dependent, N
                     type, alpha 1B subunit"
                     /codon_start=1
                     /product="voltage-dependent N-type calcium channel subunit
                     alpha-1B isoform 1"
                     /protein_id="NP_001182128.1"
                     /db_xref="GeneID:257648"
                     /db_xref="RGD:628852"
                     /translation="MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVL
                     YKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMIL
                     ATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGS
                     YLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMK
                     AMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARL
                     CDSDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWL
                     YFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWI
                     FKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAAGSPFA
                     RASLKSGKTESSSYFRRKEKMFRFLIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQP
                     QRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAA
                     IKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVF
                     ALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSK
                     GMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAK
                     EVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPQQNSAKARSVWEQRASQLRL
                     QNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPAGNKSK
                     PEGTEATEGADPPRRHHRHRDRDKTSASTPAGGEQDRTDCPKAESTETGAREERARPR
                     RSHSKEAPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGKEGTAP
                     VLVPKGERRARHRGPRTGPRETENSEEPTRRHRAKHKVPPTLEPPEREVAEKESNVVE
                     GDKETRNHQPKEPRCDLEAIAVTGVGSLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQ
                     PSDPSTTVHVPVTLTGPPGEATVVPSANTDLEGQAEGKKEAEADDVLRRGPRPIVPYS
                     SMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYM
                     DYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDI
                     NTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQ
                     LFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFT
                     VSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIIT
                     FQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYF
                     IMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSLECILKIIAFGVLNYFRDA
                     WNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTF
                     VQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDGTSINRHNNFRTFLQALMLLFR
                     SATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIM
                     DNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKC
                     PARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKE
                     ISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLS
                     QMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWG
                     TQRTQDVLYEARAPLERGHSAEIPVGQPGALAVDVQMQNMTLRGPDGEPQPGLESQGR
                     AASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCNTVLDRPPPSQVSHHHHHRCH
                     RRRDKKQRSLEKGPSLSVDTEGAPSTAAGSGLPHGEGSTGCRRERKQERGRSQERRQP
                     SSSSSEKQRFYSCDRFGSREPPQPKPSLSSHPISPTAALEPGPHPQGSGSVNGSPLMS
                     TSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLS
                     EHNALLQKEPLSQPLASGSRIGSDPYLGQRLDSEASAHNLPEDTLTFEEAVATNSGRS
                     SRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC"
     exon            409..514
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            515..654
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            655..746
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            747..899
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1090
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1194
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1310
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1367
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1460
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1670
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1783
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1896
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..2028
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2101
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2219
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2287
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2394
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2457
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2458..3246
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3247..3464
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3465..3591
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3592..3721
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3722..3781
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3782..3888
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3889..3985
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            3986..4146
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4147..4284
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4285..4486
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4487..4651
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4652..4762
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4763..4846
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4847..4852
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4853..4969
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            4970..5035
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5036..5127
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5128..5272
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5273..5400
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5401..5497
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5498..5606
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5607..5714
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5715..5827
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5828..5955
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5956..6091
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6092..6208
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6209..6413
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6414..6658
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6659..9783
                     /gene="Cacna1b"
                     /gene_synonym="BIII"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggtgcggta gcagctggcg ggcgaggtcc agacagctcg ctgcggctag gttaggagcc
       61 cctggcgcgc cgcgccctcg gtgccgggcc gcggagccgg ggatgcgcgc ggcgcccggg
      121 agtcatggtc cgcttcgggg acgagctagg cggccgctat gggggcaccg gcggcgggga
      181 gcgggctcgg ggcggcgggg ccggcggggc cggtggcccg ggccaggggg gtctgccgcc
      241 gggccagcgg gtcctgtaca agcagtccat tgcgcaacgc gcacggacca tggccctgta
      301 caaccccatc ccagtcaagc agaactgctt caccgtcaac cgctcgctct tcgtcttcag
      361 cgaggacaac gtcgtccgca aatatgctaa gcgcatcacc gaatggccgc ccttcgaata
      421 tatgatcctg gccaccatca tcgccaactg tattgtcctg gccctggagc agcacctccc
      481 tgatggggac aagactccca tgtctgaacg actggatgac acggaacctt acttcatcgg
      541 catcttttgc ttcgaggcgg gcatcaagat catagctctg ggcttcgtgt tccacaaagg
      601 ctcctacctc cggaatggct ggaacgtcat ggacttcgtg gtggtcctca cagggattct
      661 tgccacagct ggaactgact ttgatctgcg caccctgagg gctgtgcgtg tgcttaggcc
      721 cctgaagttg gtgtctggaa ttccaagctt gcaggtggtg ctcaagtcca tcatgaaggc
      781 catggtcccg ctgctgcaga tcgggctgct gctcttcttc gccatcctca tgttcgctat
      841 catcggcctc gagttctata tgggcaaatt ccataaggcc tgcttcccca acagcacaga
      901 tgcagagcct gtgggtgact ttccttgtgg caaggaggcc cctgctcgtc tgtgtgacag
      961 tgacaccgaa tgccgggagt actggccagg acccaacttt ggcatcacca attttgacaa
     1021 catcctgttt gccatcttga ccgtgttcca gtgtatcacc atggagggct ggactgacat
     1081 cctctacaat acaaatgatg cggccggcaa cacgtggaac tggttgtact tcatccccct
     1141 catcatcatt ggctccttct tcatgctcaa cctggtgctc ggtgtgcttt caggagagtt
     1201 tgccaaagag cgggagcgag tcgagaaccg ccgtgccttc ctgaagctcc gcaggcagca
     1261 gcagattgag cgagaactga atgggtactt ggagtggatc ttcaaggcgg aggaagtcat
     1321 gttggcagag gaggacaaga acgcagaaga gaagtcccct ttggatgcag tgttgaagag
     1381 agctgctacc aagaagagcc gaaatgacct catccatgca gaagaggggg aggaccggtt
     1441 tgtagacctc tgtgctgctg ggtctccctt tgctcgtgcc agcctcaaga gtgggaagac
     1501 agagagctca tcgtacttcc ggaggaagga gaagatgttc cggttcctta tccgtcgtat
     1561 ggtgaaagca cagagcttct actgggtggt actgtgcgtg gtggccctga acacgttgtg
     1621 tgtggccatg gtacactata atcagcctca gcggcttacc actgcactgt actttgcaga
     1681 gtttgttttc ctgggtctct tcctcacaga gatgtccctg aagatgtacg gtctagggcc
     1741 cagaagctac ttccggtctt ccttcaactg ctttgacttt ggggtgattg tggggagtat
     1801 ctttgaagta gtctgggctg ccatcaagcc aggaacctcc ttcggaatca gtgtgctgcg
     1861 ggctctccga ctgctgagga ttttcaaagt caccaagtat tggaactccc tgaggaacct
     1921 ggttgtttcc ctcctcaact ccatgaagtc catcatcagc cttctcttcc tgcttttcct
     1981 tttcattgtg gtcttcgctc tgttggggat gcagctgttt gggggacagt tcaactttca
     2041 agatgagact ccaaccacca attttgatac cttcccagct gccatcctca ctgtgtttca
     2101 gattctgaca ggagaggact ggaatgcagt catgtatcat gggattgagt cacaaggagg
     2161 agtcagcaaa ggcatgtttt catcctttta cttcatcgtc ctgacactgt ttggaaacta
     2221 caccctgttg aacgttttct tggccattgc tgtggacaac cttgccaatg cccaggagtt
     2281 gaccaaggat gaagaggaga tggaagaggc agccaatcag aagcttgctc ttcagaaggc
     2341 caaagaagta gctgaagtca gccccatgtc tgctgccaac atctccattg ctgcttttgt
     2401 aaagcaaact cgaggtactg tatctcgcag ctcatctgtc tccagcgtaa actcaccgca
     2461 gcagaactcg gccaaggcgc gctcagtatg ggagcagcgg gccagtcagc taaggctcca
     2521 gaacctgcgt gccagctgtg aggcactgta cagtgagatg gacccggagg agcgcctgcg
     2581 ttatgccagc acgcgccacg tgaggccaga catgaagaca cacatggacc gacccctagt
     2641 ggtggaacct ggtcgggatg gcctgcgggg acccgccggg aacaagtcaa agcctgaggg
     2701 cacggaggcc accgaaggtg cggatccacc acgccgacac caccggcatc gtgataggga
     2761 caagacctca gcctcaaccc ctgctggagg cgaacaggac aggacagact gcccaaaggc
     2821 cgaaagcacc gagaccgggg cccgggagga acgtgcgcgc cctcgtcgaa gtcacagcaa
     2881 ggaggctcca ggggctgaca cacaagtgcg ttgtgagcgc agtagacgtc accaccggcg
     2941 cggatccccg gaggaggcca ctgaacggga acctcggcgc caccgtgccc accggcacgc
     3001 acaggactca agcaaggaag gcaaggaggg cactgcaccg gtgcttgtac ccaagggcga
     3061 gcgtcgcgca agacatcgag gcccgcgtac gggcccccgt gagacagaga acagtgagga
     3121 gcccacacgc aggcaccgtg caaagcataa ggtgccacca acacttgagc ccccagagag
     3181 ggaggttgca gagaaggaga gcaacgtggt ggaaggggat aaggaaactc gaaatcacca
     3241 gcccaaggaa cctcgctgtg acctggaggc cattgcggtt acaggcgtgg gctctctgca
     3301 catgctgccc agcacctgtc tccagaaagt ggacgaacag ccagaggatg cagacaacca
     3361 gcgtaatgtc acccggatgg gcagtcagcc ctcagacccc agcaccactg tgcatgtccc
     3421 agtgacactg acaggccctc ccggggaggc cactgtagtt cccagtgcta acacggacct
     3481 ggaaggccaa gcggagggca agaaggaggc agaggctgac gatgtgctga gaagaggccc
     3541 caggcccatc gttccctaca gttccatgtt ctgcctcagc cccaccaacc tactccgtcg
     3601 cttctgccat tacattgtga ccatgcggta ctttgagatg gtgattcttg tggtcatcgc
     3661 cttgagcagc attgccctgg ctgctgagga tcccgtgcgg accgactcat tccggaacaa
     3721 tgctctgaag tacatggact acatctttac aggagtcttc acctttgaga tggtcataaa
     3781 gatgatagac ttgggcctgc tgctgcaccc tggggcctac ttccgggacc tgtggaacat
     3841 tctggacttc attgttgtca gtggagccct ggtggcattt gcattctcag gatccaaagg
     3901 gaaagacatc aataccatca agtctctgag agtcctgcga gtcctgcggc ccctcaagac
     3961 catcaagcgg ctgcctaaac tcaaggctgt gtttgactgt gtggtgaact ctctgaagaa
     4021 tgtcttgaac atcctgatcg tctacatgct cttcatgttt atatttgccg tcatcgccgt
     4081 ccaactcttc aaagggaagt tcttttactg cactgatgag tccaaggagc tggagcggga
     4141 ctgcaggggt cagtatttgg attatgagaa ggaagaggta gaagcccagc caaggcagtg
     4201 gaagaaatat gacttccact atgacaatgt gctctgggcc ttgctgactc tgtttacggt
     4261 gtccacagga gaggggtggc ccatggtgct gaaacactct gtggacgcca cctatgagga
     4321 gcaggggcca agccccgggt ttcggatgga gctttccatc ttctatgtgg tctactttgt
     4381 ggtcttccct tttttctttg tcaacatctt tgtggccttg atcatcatca ccttccagga
     4441 gcagggggac aaggtgatgt ctgagtgcag tctggaaaag aatgagaggg cttgcattga
     4501 ctttgccatc agcgccaaac ccctgacacg gtacatgcct cagaacaagc agtcgttcca
     4561 gtataagaca tggacatttg tggtctctcc accctttgag tacttcatta tggccatgat
     4621 agccctcaac acagtggtgc tgatgatgaa gttctacgat gccccttatg agtacgagct
     4681 gatgctgaag tgcttgaaca tcgtcttcac atccatgttc tctctggagt gcatcctgaa
     4741 gatcatcgcc ttcggggtgt tgaactactt cagagatgcc tggaacgtct ttgactttgt
     4801 cactgttttg ggaagtatta ctgatatttt agtaacggag attgcggaaa cgaacaactt
     4861 catcaacttg agcttccttc gcctcttccg ggcagcacgg ctgatcaagc tgcttcgcca
     4921 gggctacacc atccgcatct tgttatggac ctttgtccag tcctttaagg cgctgcccta
     4981 cgtgtgcctc ctcattgcca tgctgttctt catctacgcc atcatcggca tgcaggtttt
     5041 tggaaacatt gcccttgatg atggcaccag catcaaccga cacaacaact tccggacatt
     5101 tctgcaagcc ttaatgctgt tgttcaggag tgccactggg gaggcctggc acgaaatcat
     5161 gctgtcttgc ctgggcaacc gggcctgcga cccacatgcc aacgccagcg aatgcgggag
     5221 cgactttgcc tatttttatt ttgtctcctt catcttcctc tgttcctttc tgatgctgaa
     5281 cctctttgtt gctgtgatca tggacaattt cgaatacctc acgcgggatt cttccatcct
     5341 agggccgcac cacctcgatg aattcattcg cgtctgggct gaatacgacc cagctgcgtg
     5401 tgggcgcatc agttacaatg acatgtttga gatgctgaaa cacatgtccc cacctctggg
     5461 tttggggaag aaatgcccgg ctcgagttgc atacaagcgc ctggttcgaa tgaacatgcc
     5521 catatccaat gaggacatga cggtacactt tacatccaca ctgatggccc tcatccggac
     5581 ggcactggag atcaagcttg ccccagcggg gacaaaacag caccaatgtg atgctgagct
     5641 gaggaaggag atctcttctg tgtgggctaa tctgccccag aagactctgg acttactggt
     5701 gccaccccac aaacctgacg agatgacagt ggggaaggtc tatgcggctc tcatgatatt
     5761 tgacttctac aaacagaaca aaaccaccag agatcagact caccaagctc ctggaggcct
     5821 gtcccagatg ggtcctgttt ccctgttcca tcctctgaag gccaccctgg agcagacaca
     5881 gcccgctgtg ctccgaggag ctcgggtttt ccttcgacaa aagagtgcaa cttccctcag
     5941 caatgggggc gccatacaaa cccaggaaag tggcatcaag gagtccctgt cctggggcac
     6001 gcagaggacc caggacgtac tttatgaggc cagagcacct ctagaacgtg gccattctgc
     6061 agagatccct gtggggcagc caggagcact ggctgtagat gtccagatgc agaacatgac
     6121 attgagagga ccggatgggg agccccagcc tggcctggag agccaaggcc gagcggcctc
     6181 tatgccacgc ctggcggcag aaacacagcc ggcccctaat gccagcccca tgaagcgctc
     6241 catctccaca ctggctccac gcccgcatgg gactcagctt tgcaacacag tcctggaccg
     6301 gccacctcct agccaggtgt cccatcacca ccaccaccgc tgccaccggc gcagggacaa
     6361 gaagcagagg tccctggaaa aggggcccag cctgtctgtt gacacagaag gtgcaccaag
     6421 tactgctgca ggatctggcc tgccccatgg agaagggtcc acaggctgcc ggcgggagcg
     6481 taagcaagag cgaggccggt cccaggagcg gaggcagccc tcctcctctt cttcagagaa
     6541 gcagcgcttc tattcctgtg accgctttgg gagccgggag cccccacaac ctaagccctc
     6601 cctcagtagc caccccatat cgccaacagc ggcactagag ccaggacccc acccgcaggg
     6661 cagtggttcc gttaatggga gccccttgat gtcaacatct ggtgctagca cgccgggccg
     6721 aggtgggcgg aggcagctcc cccagactcc cctgacccca cgccccagca tcacctacaa
     6781 gacggccaat tcctcgcctg tccactttgc tgagggtcag agtggccttc cagccttctc
     6841 ccctggccgt ctcagccgcg gcctttctga acacaatgcc ctgctccaga aagagcccct
     6901 gagccagcct ctagcttctg gctcccgcat tggctctgac ccttacctag ggcagcgtct
     6961 ggacagtgag gcctctgccc acaacctgcc tgaggataca ctcacctttg aagaggccgt
     7021 ggccaccaac tctggccgct cctccaggac ttcctatgtg tcctccctca cttcccaatc
     7081 ccaccctctc cgccgtgtac ccaatggcta ccactgcact ttgggactca gcaccggcgt
     7141 ccgggcgcgg cacagctacc accacccaga ccaggatcac tggtgctagc tgcaccacga
     7201 ccacccatgc accagctcgt gggtgcgggt tccagttgat gagttttatc atccgctctg
     7261 ggttgtgcgg tcacagccct gggaggaggg tcctcacatc gcggcctctg tggtggaggt
     7321 tcctgcttct ctccctccct cccttttaca ctggacagac taataaagcc ctttcttaga
     7381 gggatatggt cctctctatc ctcctgtgta ctgccttcct gggttccatg ccagatgttg
     7441 gatcctaagc agaggtagct gagttgagat agacccagca aatccaaatc ctatgtcatg
     7501 gcctccagct tccagggtgg gtacttggga ctttcttagg aggtctgagc ctcatggaga
     7561 ttgtggtttg tccaaatgtg tggcatgggg gatagggtac cctcaaaggc aaggaaagga
     7621 gcccaactgt gtggcctggc agcacctgcc agcatcacta ctctcatgtc tattgtggtc
     7681 ttggagtcaa atagcacatg tatatagaga tatgctcaag ggcctgcctt tcacctacat
     7741 tgtcaccata atagggacca aatctagagg atgtccttgc tgttgattct ggttttcagt
     7801 cacaacactt tcactttttg tcatttctat atagttgatc tagaaaaaca gaaatcaaaa
     7861 cagggaagaa aatgttcgtg taacttaaaa aagaaatcaa cgtgtaggaa ggtctccatt
     7921 ttgcattgtt tctgtgactt gtatgcaatg ttcctgtatg tattctaccc ttcccgggaa
     7981 gtccccaatg accctggttc ctctgctcaa ccaagtgcct gatctctggc tctgagcatc
     8041 gtggctgagg tgcggcctca ggaagcatcg gggagctgct cagagcagca ctaggacttg
     8101 tgtcttaggg acactgaccg tgtccagcag catgtcagag aagcagctgt agtgcccatg
     8161 ttcctccctg agtgatgggt tctgaagaag ccagagcagc acaatgtgtg cttgcgtgag
     8221 gcactttccg ccttttaaaa tctgattctc agggatggga tgcctgccaa gtagggtgtg
     8281 atctctgttg tgttttaaaa aacaacaaca acaaacaaac aaaacctagt attcactgaa
     8341 tgctgaagag agcaaaatgc aagcaaagaa gggactgggg ttagagggag aagcccgcac
     8401 tggcagcata ataagaaact ggcagggagg ggatggtcct ggaacaggcc aggtgcctag
     8461 agctgagtcc agcccctggc ccggaactgg ggacacagca ctcaaataaa acctcatggc
     8521 tacttggtga aggcaaaccc atgctcagga aggtgttcag tgtgcagaga tggctgtgag
     8581 gccatgagag aaaggtttca cataggcagg cagtccttgg tgtgttctct gtgttttgaa
     8641 acgtctgatg acttcttggt ggactgttgg tttctacccc atgtttctca cagaagctgt
     8701 gtatatgtgt gattgcgcgt gtgattgcat gtgtgtggta gtgtgcgtgc gtgagcatgc
     8761 atgagtcata ggaaatgtgt gtgtgtgtgt gtgtgtgtgt gtaggtgtgt gtacgtgtgt
     8821 tcagcaagtg gcttttgtca accatagggc tatgcaacaa aagacacatt actagaaaca
     8881 aaacacaaga ccaccactcg gtctagggtt tcagcatgat tgtgaccaaa ccttttatag
     8941 aatttcctta tatgaaggca caataccctg aaactttaaa gataacagag tattttattc
     9001 cagtagggta agattaaaca ggaccctgga ctgcatgtga ctgcactcat gtacaacaga
     9061 ggaggatgtg cattttgata ctgttctgtc tctgtcccag ccccagccct tttctcttga
     9121 gtgttgaatg tatacattct gtgtggaact acagctgctc cagacagtcc tgggttggga
     9181 atcatcttta tcccacatta acatagctgg cttttcttcc aagcactggt acacaggaaa
     9241 ggagacatga tgtcttgctt cctgactttg ggtttgtttc tgtactgtct cttctcaaga
     9301 tgttgtctgt tccccctgaa atttcatagt gagttgccaa atttgaaatg caacaaccag
     9361 ctgtctgcat ctggaacctg tcaagcagtg ctgtagtttg aaaaagttat gtgtgcatgt
     9421 aaaatataca catatatata tatacattat acaagtatgt gcatgaaatg tatatcttca
     9481 tactttttga tacaatgtat tcatttgtta atttttaatt atatttgata taaattgaag
     9541 gtttgttgca aaaatttata tttaacagtg ttgagagaga gagaaagagc gagagaggga
     9601 gagagagaga aagatccaat catgcaacag aaatgggact actttaaaaa tcagtccttt
     9661 gactagtttg ctgcctgaat aatatttaca aaccaaactt tggattctgc tcttgtttct
     9721 acaatgactt tttgtataaa gcaaagtcct tggattaaat aaaaacaacc aaaaaaatca
     9781 aaa
//
