LOCUS       NP_058690               1663 aa            linear   ROD 09-FEB-2025
DEFINITION  complement C3 precursor [Rattus norvegicus].
ACCESSION   NP_058690 XP_346864
VERSION     NP_058690.2
DBSOURCE    REFSEQ: accession NM_016994.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1663)
  AUTHORS   Huang,Q., Su,J., Xu,J., Yu,H., Jin,X., Wang,Y., Yan,M., Yu,J.,
            Chen,S., Wang,Y. and Lv,G.
  TITLE     Beneficial effects of Panax notoginseng (Burkill) F. H. Chen flower
            saponins in rats with metabolic hypertension by inhibiting the
            activation of the renin-angiotensin-aldosterone system through
            complement 3
  JOURNAL   BMC Complement Med Ther 23 (1), 13 (2023)
   PUBMED   36653797
  REMARK    Erratum:[BMC Complement Med Ther. 2023 Mar 8;23(1):76. doi:
            10.1186/s12906-023-03893-1.. PMID: 36890530]
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1663)
  AUTHORS   Zhang,X., Chen,Y., Yu,S., Jin,B. and Liu,W.
  TITLE     Inhibition of C3a/C3aR Axis in Diverse Stages of Ulcerative Colitis
            Affected the Prognosis of UC by Modulating the Pyroptosis and
            Expression of Caspase-11
  JOURNAL   Inflammation 43 (6), 2128-2136 (2020)
   PUBMED   32617860
  REMARK    GeneRIF: Inhibition of C3a/C3aR Axis in Diverse Stages of
            Ulcerative Colitis Affected the Prognosis of UC by Modulating the
            Pyroptosis and Expression of Caspase-11.
            Erratum:[Inflammation. 2021 Jun;44(3):1203-1204. doi:
            10.1007/s10753-020-01380-0.. PMID: 33174140]
REFERENCE   3  (residues 1 to 1663)
  AUTHORS   Ramlall,V., Thangaraj,P.M., Meydan,C., Foox,J., Butler,D., Kim,J.,
            May,B., De Freitas,J.K., Glicksberg,B.S., Mason,C.E.,
            Tatonetti,N.P. and Shapira,S.D.
  TITLE     Immune complement and coagulation dysfunction in adverse outcomes
            of SARS-CoV-2 infection
  JOURNAL   Nat Med 26 (10), 1609-1615 (2020)
   PUBMED   32747830
REFERENCE   4  (residues 1 to 1663)
  AUTHORS   Zhang,X., Tan,Y., Ling,Y., Lu,G., Liu,F., Yi,Z., Jia,X., Wu,M.,
            Shi,B., Xu,S., Chen,J., Wang,W., Chen,B., Jiang,L., Yu,S., Lu,J.,
            Wang,J., Xu,M., Yuan,Z., Zhang,Q., Zhang,X., Zhao,G., Wang,S.,
            Chen,S. and Lu,H.
  TITLE     Viral and host factors related to the clinical outcome of COVID-19
  JOURNAL   Nature 583 (7816), 437-440 (2020)
   PUBMED   32434211
REFERENCE   5  (residues 1 to 1663)
  AUTHORS   Wang,J., Zhang,Y., Liu,Y.M., Yang,X.C., Chen,Y.Y., Wu,G.J.,
            He,X.H., Duan,L., Dong,Y. and Ma,R.F.
  TITLE     Uncovering the protective mechanism of Huoxue Anxin Recipe against
            coronary heart disease by network analysis and experimental
            validation
  JOURNAL   Biomed Pharmacother 121, 109655 (2020)
   PUBMED   31734577
REFERENCE   6  (residues 1 to 1663)
  AUTHORS   Milot,J., Fremont,P., Cote,C. and Tremblay,R.R.
  TITLE     Differential modulation of carbonic anhydrase (CA III) in slow- and
            fast-twitch skeletal muscles of rat following denervation and
            reinnervation
  JOURNAL   Biochem Cell Biol 69 (10-11), 702-710 (1991)
   PUBMED   1799436
REFERENCE   7  (residues 1 to 1663)
  AUTHORS   Botto,M., Fong,K.Y., So,A.K., Rudge,A. and Walport,M.J.
  TITLE     Molecular basis of hereditary C3 deficiency
  JOURNAL   J Clin Invest 86 (4), 1158-1163 (1990)
   PUBMED   2212005
REFERENCE   8  (residues 1 to 1663)
  AUTHORS   Jacobs,J.W., Rubin,J.S., Hugli,T.E., Bogardt,R.A., Mariz,I.K.,
            Daniels,J.S., Daughaday,W.H. and Bradshaw,R.A.
  TITLE     Purification, characterization, and amino acid sequence of rat
            anaphylatoxin (C3a)
  JOURNAL   Biochemistry 17 (23), 5031-5038 (1978)
   PUBMED   309768
REFERENCE   9  (residues 1 to 1663)
  AUTHORS   Sahashi,K., Engel,A.G., Linstrom,J.M., Lambert,E.H. and Lennon,V.A.
  TITLE     Ultrastructural localization of immune complexes (IgG and C3) at
            the end-plate in experimental autoimmune myasthenia gravis
  JOURNAL   J Neuropathol Exp Neurol 37 (2), 212-223 (1978)
   PUBMED   147324
REFERENCE   10 (residues 1 to 1663)
  AUTHORS   Coonrod,J.D. and Rylko-Bauer,B.
  TITLE     Complement levels in pneumococcal pneumonia
  JOURNAL   Infect Immun 18 (1), 14-22 (1977)
   PUBMED   20405
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            X52477.1, JAXUCZ010000009.1, CK366193.1, CO569380.1, CO800063.1,
            CO796620.1, CO565011.1 and BI281644.1.
            
            On Oct 11, 2007 this sequence version replaced NP_058690.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X52477.1, SRR23984936.756514.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Rattus norvegicus"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q11"
     Protein         1..1663
                     /product="complement C3 precursor"
                     /EC_number="3.4.21.43"
                     /note="complement component 3"
                     /calculated_mol_wt=183975
     sig_peptide     1..24
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2368
     mat_peptide     25..1663
                     /product="Complement C3. /id=PRO_0000005937"
                     /note="propagated from UniProtKB/Swiss-Prot (P01026.3)"
                     /calculated_mol_wt=183975
     Region          26..124
                     /region_name="MG1"
                     /note="Macroglobulin domain MG1; pfam17790"
                     /db_xref="CDD:465508"
     Site            40
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P01024; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Region          <128..1345
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Site            297
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P01024; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Site            303
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P01024; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Site            671
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Site            747..748
                     /site_type="cleavage"
                     /note="Cleavage, by carboxypeptidases.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Site            748..749
                     /site_type="cleavage"
                     /note="Cleavage, by C3 convertase; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Site            939
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000305; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Site            959..960
                     /site_type="cleavage"
                     /note="Cleavage, by factor I. /evidence=ECO:0000255;
                     propagated from UniProtKB/Swiss-Prot (P01026.3)"
     Site            968
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P01024; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Region          996..1282
                     /region_name="complement_C3_C4_C5"
                     /note="Proteins similar to C3, C4 and C5 of vertebrate
                     complement. The vertebrate complement system, comprised of
                     a large number of distinct plasma proteins, is an effector
                     of both the acquired and innate immune systems. The point
                     of convergence of the...; cd02896"
                     /db_xref="CDD:239226"
     Site            order(1010,1012..1013,1016,1059,1069,1072,1123,1125..1126,
                     1266)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239226"
     Site            order(1010..1013,1126)
                     /site_type="active"
                     /db_xref="CDD:239226"
     Site            1010..1013
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239226"
     Site            order(1112,1116,1118,1126,1131..1132)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239226"
     Site            1303..1304
                     /site_type="cleavage"
                     /note="Cleavage, by factor I. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (P01026.3)"
     Site            1320..1321
                     /site_type="cleavage"
                     /note="Cleavage, by factor I. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (P01026.3)"
     Site            1321
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P01024; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Region          1397..1494
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     Region          1513..1661
                     /region_name="NTR_complement_C3"
                     /note="NTR/C345C domain, complement C3 subfamily; The NTR
                     domain found in complement C3 is also known as the C345C
                     domain because it occurs at the C-terminus of complement
                     C3, C4 and C5. Complement C3 plays a pivotal role in the
                     activation of the complement...; cd03583"
                     /db_xref="CDD:239638"
     Site            1573
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P01024; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Site            1617
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000305; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     Region          1634..1659
                     /region_name="Interaction with CFP/properdin.
                     /evidence=ECO:0000250|UniProtKB:P01024"
                     /note="propagated from UniProtKB/Swiss-Prot (P01026.3)"
     Site            1663
                     /site_type="other"
                     /note="Coordinates Mg2+ for interaction with Complement
                     factor B Bb fragment.
                     /evidence=ECO:0000250|UniProtKB:P01024; propagated from
                     UniProtKB/Swiss-Prot (P01026.3)"
     CDS             1..1663
                     /gene="C3"
                     /coded_by="NM_016994.2:42..5033"
                     /db_xref="GeneID:24232"
                     /db_xref="RGD:2232"
ORIGIN      
        1 mgptsgsqll vlllllassl lalgspmysi itpnvlrles eetfileahd aqgdvpvtvt
       61 vqdflkkqvl tsektvltga tghlnrvsik ipaskefnad kghkyvtvva nfgatvveka
      121 vlvsfqsgyl fiqtdktiyt pgstvfyrif tvdnnllpvg ktvvivietp dgvpikrdil
      181 sshnqygilp lswnipelvn mgqwkirafy ehapkqtfsa efevkeyvlp sfevlvepte
      241 kfyyiddpkg levsitarfl ygknvdgtaf vifgvqdedk kislaqsltr vliedgsgea
      301 vlsrkvlmdg vrpsspealv gkslyvsvtv ilhsgsdmve aersgipivt spyqihftkt
      361 pkffkpampf dlmvfvtnpd gsparrvpvv tqgsdaqalt qddgvaklsv ntpnnrqplt
      421 itvrtkkegi pdarqatrtm qaqpystmhn snnylhlsvs rvelkpgdnl nvnfhlrtda
      481 gqeakiryyt ylvmnkgkll kagrqvrepg qdlvvlslpi tpefipsfrl vayytligan
      541 gqrevvadsv wvdvkdscvg tlvvkgdprd nrqpapghqt tlriegnqga rvglvavdkg
      601 vfvlnkknkl tqskiwdvve kadigctpgs gknyagvfmd agltfktnqg lqtdqredpe
      661 cakpaarrrr svqlmerrmd kagqytdkgl rkccedgmrd ipmkyscqrr arlitqgesc
      721 lkafmdccny itklreqhrr dhvlglarsd vdediipeed iisrshfpes wlwtieelke
      781 pekngistkv mniflkdsit tweilavsls dkkgicvadp yeitvmqdff idlrlpysvv
      841 rneqveirav lfnyreqekl kvrvellhnp afcsmatakk ryyqtieipp kssvavpyvi
      901 vplkiglqev evkaavfnhf isdgvkkilk vvpegmrvnk tvavrtldpe hlgqggvqre
      961 dvpaadlsdq vpdtdsetri llqgtpvaqm aedavdgerl khlivtpsgc geqnmigmtp
     1021 tviavhyldq teqwekfgle krqealelik kgytqqlafk qpssayaafn nrppstwlta
     1081 yvvkvfslaa nliaidsqvl cgavkwlile kqkpdgvfqe dgpvihqemi ggfrntkead
     1141 vsltafvlia lqeardiceg qvnslpgsin kageyleasy lnlqrpytva iagyalalmn
     1201 kleepyltkf lntakdrnrw eepgqqlynv eatsyallal lllkdfdsvp pvvrwlneqr
     1261 yygggygstq atfmvfqala qyqtdvpdhk dlnmdvslhl psrssptvfr llwesgsllr
     1321 seetkqnegf sltakgkgqg tlsvvtvyha kvkgkatckk fdlrvtikpa petakkpqda
     1381 kssmildict rylgdvdatm sildismmtg fipdtndlel lssgvdryis kyemdkafsn
     1441 kntliiylek ishseedcls fkvhqffnvg liqpgsvkvy syynleesct rfyhpekddg
     1501 mlsklchnem crcaeencfm hqsqdqvsln erldkacepg vdyvyktklt tielsddfde
     1561 yimtieqvik sgsdevqagq errfishvkc rnalklqkgk qylmwglssd lwgekpntsy
     1621 iigkdtwveh wpeaeecqdq knqkqcedlg aftetmvvfg cpn
//
