LOCUS       NM_138900               3082 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus complement C1s (C1s), mRNA.
ACCESSION   NM_138900 XM_001062258
VERSION     NM_138900.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Bastos-Amador,P., Royo,F., Gonzalez,E., Conde-Vancells,J.,
            Palomo-Diez,L., Borras,F.E. and Falcon-Perez,J.M.
  TITLE     Proteomic analysis of microvesicles from plasma of healthy donors
            reveals high individual variability
  JOURNAL   J Proteomics 75 (12), 3574-3584 (2012)
   PUBMED   22516433
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Gregory,L.A., Thielens,N.M., Arlaud,G.J., Fontecilla-Camps,J.C. and
            Gaboriaud,C.
  TITLE     X-ray structure of the Ca2+-binding interaction domain of C1s.
            Insights into the assembly of the C1 complex of complement
  JOURNAL   J Biol Chem 278 (34), 32157-32164 (2003)
   PUBMED   12788922
REFERENCE   3  (bases 1 to 3082)
  AUTHORS   Nakagawa,M., Nakashima,S., Banno,Y., Yamada,J., Sawada,M.,
            Yoshimura,Si., Kaku,Y., Iwama,T., Shinoda,J. and Sakai,N.
  TITLE     Increased expression and secretion of r-Gsp protein, rat
            counterpart of complement C1s precursor, during cyclic AMP-induced
            differentiation in rat C6 glioma cells
  JOURNAL   Brain Res Mol Brain Res 106 (1-2), 12-21 (2002)
   PUBMED   12393260
  REMARK    GeneRIF: These results suggest that r-Gsp protein could not induce
            glial differentiation alone, and suggest that r-Gsp protein was
            secreted as a proenzyme and processed in culture medium [rGsp
            protein].
REFERENCE   4  (bases 1 to 3082)
  AUTHORS   Rossi,V., Cseh,S., Bally,I., Thielens,N.M., Jensenius,J.C. and
            Arlaud,G.J.
  TITLE     Substrate specificities of recombinant mannan-binding
            lectin-associated serine proteases-1 and -2
  JOURNAL   J Biol Chem 276 (44), 40880-40887 (2001)
   PUBMED   11527969
REFERENCE   5  (bases 1 to 3082)
  AUTHORS   Sakai,H., Nakashima,S., Yoshimura,S., Nishimura,Y., Sakai,N. and
            Nozawa,Y.
  TITLE     Molecular cloning of a cDNA encoding a serine protease homologous
            to complement C1s precursor from rat C6 glial cells and its
            expression during glial differentiation
  JOURNAL   Gene 209 (1-2), 87-94 (1998)
   PUBMED   9524231
REFERENCE   6  (bases 1 to 3082)
  AUTHORS   Thielens,N.M., Aude,C.A., Lacroix,M.B., Gagnon,J. and Arlaud,G.J.
  TITLE     Ca2+ binding properties and Ca2(+)-dependent interactions of the
            isolated NH2-terminal alpha fragments of human complement proteases
            C1-r and C1-s
  JOURNAL   J Biol Chem 265 (24), 14469-14475 (1990)
   PUBMED   2387866
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Dec 2, 2020 this sequence version replaced NM_138900.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D88250.1, BC062042.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-303               JAXUCZ010000004.1  159128434-159128736 c
            304-438             JAXUCZ010000004.1  159127483-159127617 c
            439-646             JAXUCZ010000004.1  159126919-159127126 c
            647-824             JAXUCZ010000004.1  159126442-159126619 c
            825-950             JAXUCZ010000004.1  159123732-159123857 c
            951-1150            JAXUCZ010000004.1  159122763-159122962 c
            1151-1304           JAXUCZ010000004.1  159121613-159121766 c
            1305-1420           JAXUCZ010000004.1  159120722-159120837 c
            1421-1499           JAXUCZ010000004.1  159120276-159120354 c
            1500-1628           JAXUCZ010000004.1  159119605-159119733 c
            1629-1703           JAXUCZ010000004.1  159118758-159118832 c
            1704-3082           JAXUCZ010000004.1  159116549-159117927 c
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..3082
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /note="complement C1s"
                     /db_xref="GeneID:192262"
                     /db_xref="RGD:619983"
     exon            1..303
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            304..438
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     CDS             416..2500
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /EC_number="3.4.21.42"
                     /note="complement C1s subcomponent; C1 esterase;
                     complement component 1 subcomponent s; complement
                     component 1, s subcomponent"
                     /codon_start=1
                     /product="complement C1s subcomponent precursor"
                     /protein_id="NP_620255.2"
                     /db_xref="GeneID:192262"
                     /db_xref="RGD:619983"
                     /translation="MGKSPEMWCFVFFSLLASFSAEPTMYGEILSPNYPQAYPNEVVK
                     TWDIEVPEGFGIHLYFTHLDMELSENCAYDSVQIISGGIEEERLCGQRTSKSPNSPIV
                     EEFQFPYNRLQVVFTSDFSNEERFTGFAAYYSAVDVNECTDFTDVPCSHFCNNFIGGY
                     FCSCPPEYFLHDDMRTCGVNCSGDVFTALIGEIASPNYPNPYPENSRCEYQIRLQEGF
                     RLVLTIRREDFDVEPADSEGNCHDSLTFAAKNQQFGPYCGNGFPGPLTIKTQSNTLDI
                     VFQTDLTGQNKGWKLRYHGDPIPCPKEISANSIWEPEKAKYVFKDVVKITCVDGFEVV
                     EGNVGSTSFYSTCQSNGQWSNSRLECQPVDCGVPEPIENGKVEDPEDTVFGSVIHYTC
                     EEPYYYMEQEEGGEYHCAANGSWVNDQLGVELPKCIPVCGVPTEPFKVQQRIFGGYST
                     KIQSFPWQVYFESPRGGGALIDEYWVLTAAHVVEGNSDPVMYVGSTLLKIERLRNAQR
                     LITERVIIHPSWKQEDDLNTRTNFDNDIALVQLKDPVKMGPTVAPICLPETSSDYNPS
                     EGDLGLISGWGRTENRTNVIQLRGAKLPITSLEKCQQVKVENPKARSNDYVFTDNMIC
                     AGEKGVDSCEGDSGGAFALPVPNVKDPKFYVAGLVSWGKKCGTYGIYTKVKNYVDWIL
                     KTMQENSGPKKD"
     exon            439..646
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            647..824
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            825..950
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1150
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1304
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1420
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1499
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1628
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1703
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..3082
                     /gene="C1s"
                     /gene_synonym="r-gsp"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 attcccatct tttctcggga tctctcaacc cagggaagct gatgcagttc ttgttttgaa
       61 agcagaggga gctcgaagat caaggccggt tacactttcc taggttgtgg tctcccagcc
      121 cccgctgttt ggaacaggga atttcggaca tgcacaacac actgtggtag ggaatcctag
      181 agcagacaga acagggaggt ggggcggagg ttcctgcaga gccctctgga gccgcagggg
      241 cttcacggca tgaccagaag caggagagga ggctgaccca cttgttccca tcagctcctg
      301 aaggtgacac tgagccctgg gtggcccctc actgccaaag cagtcacctg tatttgtcag
      361 ataaagacag ccagcccggc tgccctttac ctccaagtca gagatccaga gagccatggg
      421 caaatcgcca gagatgtggt gctttgtctt cttttctctt ttggcatcgt tttctgctga
      481 gcctaccatg tatggggaga tcctgtcccc taattatccc caggcgtacc ccaatgaggt
      541 cgtgaaaact tgggacatag aagtcccaga ggggtttggg attcaccttt acttcaccca
      601 tctggacatg gagctgtcag agaactgtgc atacgactca gtgcagataa tctcaggagg
      661 catcgaggaa gagagactct gtggccagag gaccagcaag agtcccaact cccccattgt
      721 agaagagttt caattcccat acaataggct ccaggtggtc tttacgtcag acttctccaa
      781 cgaggaacgg tttactggct ttgcagcgta ttactcagcc gtagatgtaa atgaatgcac
      841 agactttaca gatgtccctt gcagccactt ctgcaataac ttcattggtg gatacttctg
      901 ctcctgcccc ccagaatact tcctccacga tgacatgagg acttgtgggg tcaactgtag
      961 tggggatgta ttcactgccc tgattgggga gatcgcaagt cccaattatc ccaacccata
     1021 cccggagaac tcaaggtgtg aataccagat tcggctgcag gagggcttcc gactggtgtt
     1081 gactatccgg agagaagatt ttgatgtgga accagctgac tcagagggga actgccacga
     1141 cagtttgact tttgctgcaa aaaaccaaca gtttggtcct tactgtggca atggattccc
     1201 tggaccgcta actattaaaa cccagagcaa tactcttgat attgtctttc aaactgacct
     1261 aacggggcaa aataaaggct ggaagcttcg ttaccatggg gatcccatcc cctgtcccaa
     1321 agaaatcagt gctaattcta tctgggagcc cgaaaaggca aaatacgtgt tcaaagatgt
     1381 cgtgaagata acctgtgtgg atggattcga agttgtggag ggaaatgttg gctcaacatc
     1441 attctattcc acttgtcaaa gcaacggaca gtggagcaat tccaggctag agtgtcaacc
     1501 tgtggactgt ggtgttccag aacccattga gaatggtaaa gttgaagacc cagaagacac
     1561 tgtattcggc tccgtcatcc actacacgtg cgaagagcca tattactaca tggaacagga
     1621 agaaggcgga gagtatcact gcgctgctaa tgggagctgg gtgaatgacc agctgggtgt
     1681 cgagcttcca aaatgtattc cagtctgtgg agtacccacc gagcccttta aagtacagca
     1741 gaggatattt ggaggatact ctacaaagat tcaaagtttt ccttggcagg tctactttga
     1801 gtccccccga ggtggcgggg ctcttatcga tgagtactgg gtgctgacgg ccgctcacgt
     1861 tgtggaggga aactctgacc cagtgatgta tgtcgggtcc acacttctga aaatagagcg
     1921 gttgagaaat gcccagaggc tcatcactga acgtgtgatt attcatccca gctggaaaca
     1981 agaggacgac ctgaatacac ggacaaattt tgacaatgac attgccctgg tgcagctcaa
     2041 agaccctgtg aaaatgggac ccactgttgc ccccatctgc ctgccagaaa cctcctcaga
     2101 ctacaacccc tcagagggtg acctggggct gatctctggg tggggccgaa cagagaatag
     2161 aaccaatgtt attcaactca gaggggcgaa gttacccata acatctttag aaaagtgcca
     2221 gcaggtgaaa gtggaaaacc cgaaagcgag gtcaaacgac tatgttttca ctgacaacat
     2281 gatctgtgct ggggaaaagg gtgtggacag ctgtgagggt gacagcggag gggcttttgc
     2341 tctgccggtc cccaatgtca aggaccccaa attctatgtg gctggcctgg tgtcctgggg
     2401 gaaaaagtgt gggacctatg ggatctacac aaaggtaaag aactacgtgg actggatcct
     2461 gaaaactatg caggagaata gtgggcccaa gaaggactga tccgtaggtg acaacacccc
     2521 tccagggcat agcaaggtca tttttctcag atcctgggac ggtcccatta tttcgaaatg
     2581 atggagagag ggtgtgggag catggttaac gttgaacatg attgtcaaga agcctgctta
     2641 gaggcagagt tgatcactga gccgtgttgg ttattcagtt gctattgcta acaacatggc
     2701 ggaagccttt ctgtcttgct tcatcccaca gggatatctt aaacgatttc cccctcattt
     2761 aacctgcttg aaatccttat tgcttacagt aaagcatgtt tccaatttgg ttctggctgg
     2821 ctcgagagcc acagaaggag agggaaattt gagggtattt tgtcatggaa ttcaggcatc
     2881 aacaggttgt ctgaaacact atgcagtcag ggaacacagc cttttttcta agtgagattt
     2941 acccaatagc tggaagtcag aattgactac cttagctttc ctttgtgagt tgtttcaata
     3001 tgtttcctag aaattagttt tcttataatc ctcctttgta tcatacaatg taatgactta
     3061 gtaaaagaga aattgaacat tg
//
