LOCUS       NM_001419566            4558 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus BR serine/threonine kinase 2 (Brsk2), mRNA.
ACCESSION   NM_001419566 XM_039101277
VERSION     NM_001419566.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Nie,J., Sun,C., Chang,Z., Musi,N. and Shi,Y.
  TITLE     SAD-A Promotes Glucose-Stimulated Insulin Secretion Through
            Phosphorylation and Inhibition of GDIalpha in Male Islet beta Cells
  JOURNAL   Endocrinology 159 (8), 3036-3047 (2018)
   PUBMED   29873699
  REMARK    GeneRIF: Glucose and incretin stimulate insulin secretion through
            the phosphorylation of GDIalpha at Ser174 by SAD-A, which leads to
            the activation of Rho GTPases, culminating in insulin exocytosis.
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Sample,V., Ramamurthy,S., Gorshkov,K., Ronnett,G.V. and Zhang,J.
  TITLE     Polarized activities of AMPK and BRSK in primary hippocampal
            neurons
  JOURNAL   Mol Biol Cell 26 (10), 1935-1946 (2015)
   PUBMED   25788287
REFERENCE   3  (bases 1 to 4558)
  AUTHORS   Wang,Y., Wan,B., Zhou,J., Li,R. and Yu,L.
  TITLE     BRSK2 is a valosin-containing protein (VCP)-interacting protein
            that affects VCP functioning in endoplasmic reticulum-associated
            degradation
  JOURNAL   Biotechnol Lett 35 (12), 1983-1989 (2013)
   PUBMED   23907667
REFERENCE   4  (bases 1 to 4558)
  AUTHORS   Chen,X.Y., Gu,X.T., Saiyin,H., Wan,B., Zhang,Y.J., Li,J.,
            Wang,Y.L., Gao,R., Wang,Y.F., Dong,W.P., Najjar,S.M., Zhang,C.Y.,
            Ding,H.F., Liu,J.O. and Yu,L.
  TITLE     Brain-selective kinase 2 (BRSK2) phosphorylation on PCTAIRE1
            negatively regulates glucose-stimulated insulin secretion in
            pancreatic beta-cells
  JOURNAL   J Biol Chem 287 (36), 30368-30375 (2012)
   PUBMED   22798068
REFERENCE   5  (bases 1 to 4558)
  AUTHORS   Li,R., Wan,B., Zhou,J., Wang,Y., Luo,T., Gu,X., Chen,F. and Yu,L.
  TITLE     APC/C(Cdh1) targets brain-specific kinase 2 (BRSK2) for degradation
            via the ubiquitin-proteasome pathway
  JOURNAL   PLoS One 7 (9), e45932 (2012)
   PUBMED   23029325
REFERENCE   6  (bases 1 to 4558)
  AUTHORS   Witte,H., Neukirchen,D. and Bradke,F.
  TITLE     Microtubule stabilization specifies initial neuronal polarization
  JOURNAL   J Cell Biol 180 (3), 619-632 (2008)
   PUBMED   18268107
REFERENCE   7  (bases 1 to 4558)
  AUTHORS   Barnes,A.P., Lilley,B.N., Pan,Y.A., Plummer,L.J., Powell,A.W.,
            Raines,A.N., Sanes,J.R. and Polleux,F.
  TITLE     LKB1 and SAD kinases define a pathway required for the polarization
            of cortical neurons
  JOURNAL   Cell 129 (3), 549-563 (2007)
   PUBMED   17482548
REFERENCE   8  (bases 1 to 4558)
  AUTHORS   Kishi,M., Pan,Y.A., Crump,J.G. and Sanes,J.R.
  TITLE     Mammalian SAD kinases are required for neuronal polarization
  JOURNAL   Science 307 (5711), 929-932 (2005)
   PUBMED   15705853
REFERENCE   9  (bases 1 to 4558)
  AUTHORS   Lu,R., Niida,H. and Nakanishi,M.
  TITLE     Human SAD1 kinase is involved in UV-induced DNA damage checkpoint
            function
  JOURNAL   J Biol Chem 279 (30), 31164-31170 (2004)
   PUBMED   15150265
REFERENCE   10 (bases 1 to 4558)
  AUTHORS   Lizcano,J.M., Goransson,O., Toth,R., Deak,M., Morrice,N.A.,
            Boudeau,J., Hawley,S.A., Udd,L., Makela,T.P., Hardie,D.G. and
            Alessi,D.R.
  TITLE     LKB1 is a master kinase that activates 13 kinases of the AMPK
            subfamily, including MARK/PAR-1
  JOURNAL   EMBO J 23 (4), 833-843 (2004)
   PUBMED   14976552
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Apr 11, 2023 this sequence version replaced XM_039101277.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760389,
                              SAMEA5760393 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-475               JAXUCZ010000001.1  206465040-206465514
            476-570             JAXUCZ010000001.1  206495202-206495296
            571-656             JAXUCZ010000001.1  206496620-206496705
            657-797             JAXUCZ010000001.1  206498051-206498191
            798-914             JAXUCZ010000001.1  206498504-206498620
            915-948             JAXUCZ010000001.1  206499167-206499200
            949-1017            JAXUCZ010000001.1  206499349-206499417
            1018-1164           JAXUCZ010000001.1  206499532-206499678
            1165-1196           JAXUCZ010000001.1  206501082-206501113
            1197-1361           JAXUCZ010000001.1  206501410-206501574
            1362-1459           JAXUCZ010000001.1  206501770-206501867
            1460-1610           JAXUCZ010000001.1  206501940-206502090
            1611-1671           JAXUCZ010000001.1  206505982-206506042
            1672-1879           JAXUCZ010000001.1  206506891-206507098
            1880-1928           JAXUCZ010000001.1  206507504-206507552
            1929-2052           JAXUCZ010000001.1  206510684-206510807
            2053-2233           JAXUCZ010000001.1  206512177-206512357
            2234-2323           JAXUCZ010000001.1  206512434-206512523
            2324-2371           JAXUCZ010000001.1  206514736-206514783
            2372-4558           JAXUCZ010000001.1  206515970-206518156
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q41"
     gene            1..4558
                     /gene="Brsk2"
                     /note="BR serine/threonine kinase 2"
                     /db_xref="GeneID:293631"
                     /db_xref="RGD:1566256"
     exon            1..475
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             382..2541
                     /gene="Brsk2"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.11.26"
                     /note="BR serine/threonine-protein kinase 2; brain
                     serine/threonine kinase 2; serine/threonine-protein kinase
                     SAD-A; brain-specific serine/threonine-protein kinase 2;
                     serine/threonine-protein kinase BRSK2-like"
                     /codon_start=1
                     /product="serine/threonine-protein kinase BRSK2"
                     /protein_id="NP_001406495.1"
                     /db_xref="GeneID:293631"
                     /db_xref="RGD:1566256"
                     /translation="MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQ
                     KVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGG
                     ELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDERNNIRIAD
                     FGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFD
                     DDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKN
                     EPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMI
                     YFLLLDRKERYPSHEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDG
                     GSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPV
                     HTPKESPAGTPNPTPPSSPSVGGVPWRTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSN
                     LTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVI
                     SQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFK
                     RVVETIQAQLLSTHDQPSAQHLSDTTNCMEVMTGRLSKCDEKNGQAAQAPSTPAKRSA
                     HGPLGDSAAAGPGGDTEYPMGKDMAKMGPPAARREQP"
     exon            476..570
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            571..656
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            657..797
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            798..914
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            915..948
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1017
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1164
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1196
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1361
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1459
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1610
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1671
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1879
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..1928
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2052
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2233
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2323
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2324..2371
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..4558
                     /gene="Brsk2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 actcgaagcg actcggctgc tcgtctgggc tcggctgagt ggctcggttc ggttgctcgg
       61 cggggctcgg ctgcacggcc gcggacgggc gtgcgctggg gggcgcgggg cgcgggcctc
      121 ggaggtggcg gcggaagcct ggtgcccccg cccgccgggt cctctcggag agggagagag
      181 gcatccccgc ggcccggcct cgcacgggtg cggcgggcgc ggcgtggggg gcggcggggg
      241 cgcggagcag cgcggcccgc ccgggggcgc cgcgggccgg gtcagcgcgg acggcgctcg
      301 gcggactcgg acgcacggct ggccggcccc tccctgcccg cgcgcccggg cgcccactcg
      361 ccggcgcggg gcccgggagc gatgacatcg acggggaagg acggcggcgg ggcgcagcac
      421 gcgcagtatg tggggcccta ccggctggag aagacgctgg gcaaggggca gacaggcttg
      481 gtgaaactgg gaatccactg tgtcacctgc cagaaggtcg ccatcaaaat cgtcaaccgt
      541 gagaagctca gtgagtcggt gctgatgaag gtggagcgag agattgccat cttgaagctc
      601 atcgaacatc cacatgtact gaagctgcat gacgtctatg aaaacaaaaa atatttatac
      661 ctggtgctag aacatgtgtc tgggggagag ctgttcgact acctggtgaa gaagggccgg
      721 ctgaccccca aggaggcccg caagttcttc cggcagatca tctctgcact ggacttctgc
      781 cacagccact ccatatgcca tagagacttg aagccagaga acctgctgct ggatgagagg
      841 aacaacatcc gtattgcaga ctttggcatg gcgtccctgc aggtgggaga cagcctgctg
      901 gagaccagct gcggatctcc acactatgcc tgtccggaag tgattcgggg cgagaagtat
      961 gatggccgca aggcagatgt gtggagctgt ggtgtgatcc tgttcgcctt gctggtgggg
     1021 gctctgccgt ttgatgatga caacctgcgg cagttgctgg agaaggtcaa gcgtggtgtg
     1081 ttccacatgc cacacttcat cccaccagac tgccagagcc tcctgcgtgg catgattgag
     1141 gtggatgcag ctcggcgcct cacgctagag cacattcaga aacacatatg gtatataggt
     1201 ggcaagaatg agccagagcc ggaacagccc atcccacgca aggtgcagat ccgctcactg
     1261 cccagcctgg aagacattga ccctgatgta ctggacagca tgcattcact gggctgcttc
     1321 cgagaccgca acaagctgct gcaggacctg ctatctgagg aggagaatca ggaaaagatg
     1381 atttatttcc tcctcctgga ccggaaagaa cggtacccca gccatgagga tgaggacctg
     1441 ccccccagga atgagataga ccctcctcgg aagcgcgtgg actccccgat gctgaaccgg
     1501 catggcaaga ggcgacctga gcgcaagtcc atggaggtgc tcagtgtgac agatggcggc
     1561 tccccagtgc ctgcacggag agccattgag atggcccagc atggccagag atctcgatcc
     1621 atcagtggtg cctcctcagg cctttctaca agtccactca gcagtcctcg ggtgacccct
     1681 cacccctcac caaggggtag tccccttcct acccccaaag ggacgcctgt tcacacgcca
     1741 aaggagagcc cagctggcac acccaacccc acaccaccgt ccagccctag tgttggagga
     1801 gtgccctggc ggacacgact caactccatc aagaacagct tcctgggctc acctcgcttc
     1861 caccgccgga aactgcaagt tcccacgcca gaggagatgt ccaacctgac cccagaatct
     1921 tctccagagc tggccaagaa atcatggttc gggaacttca tcaacctgga aaaggaggaa
     1981 cagatctttg tggtgatcaa ggacaagccc ctgagctcca tcaaggctga catcgtgcat
     2041 gccttcctgt cgatccctag cctcagccac agcgttatct cccagacaag cttcagggct
     2101 gaatacaagg ccacaggggg cccagcagtg ttccagaagc cggtcaagtt ccaggtggac
     2161 atcacctaca ctgagggtgg agaggcccag aaggagaatg gcatctactc agtcacattc
     2221 actttactct caggccccag tcgccgcttc aagagggtag tggagaccat ccaggcccag
     2281 ctgctgagca cccatgacca gccatcagcc cagcacctgt cagacaccac taactgtatg
     2341 gaagtgatga cggggcggct ttccaaatgt gacgagaaga acgggcaggc ggcccaggcc
     2401 cccagcacac ccgccaagcg gagtgcccac ggccccctgg gtgactccgc ggccgctggc
     2461 cctggagggg acaccgagta cccgatgggc aaggacatgg ccaagatggg gccgcccgcc
     2521 gcccgccgtg agcagcctta gacactagtc cctccccagc acagcacagc acagcacaac
     2581 acagcactga ctgtggcctt ctgccgccct gtgtggacca ggccccacct gcctgcccag
     2641 accttgtctc aagcctggga ggaaaggaga gtggccaggg tgcggcccgc aggctgggct
     2701 gctgggcttg ctctcttttc tctcttgcgg tctctgtctc tgcctgtctc tgacagcatc
     2761 gcttgtttcc gctctgatac caggaattat cccgaaaagt taacatgtca cctccacgag
     2821 gccatcctct gtgctgcgtg tggacactgg ctgaccgaag gcagctgctg cggaccaccc
     2881 tcctcctcct gctgctgctg ctgctgctgc tgggcagaga ggctagccca ctgtccacca
     2941 cctctgcctg ttccctggca gctccgcatg gcccaaggga ggagggcctg cctcccaggg
     3001 aattcctctt ctagcccagc caccctggat tcccagtgac cagatcctca gcagggacaa
     3061 cctgtccacc atcttccagg gtaggggacc atggtgggtg aagcagtggg aatggattgt
     3121 cctccacagc gcatttggag gcctcctggg ccattcacct cgccttttcc acacgttggc
     3181 ctcaccaggg ccacctcttc tcatctgcat ccattgctcc accttgccag gctgcagagc
     3241 tccagctgtg atggttctca tgggctgaag tggagataaa agacctgggt gctccaaagc
     3301 tgccaggggc ctgggctgag accagtatta tttatttgct gttttaattt attgagtttc
     3361 tttatgtctc tgttctctgt tcagggaagg gggagtagca gcagcgccct cctgccatct
     3421 gtctgtctgt tctgtctgtc tgacttgggc agggtgggtc ctgagcccaa gttccctctg
     3481 aggacagagc cgttgtgggg ctggggacag tggggagcca gccagtggat gcagcactgg
     3541 caagctactg taaactttaa aagaattcct gcaagatatt tttataaact ttcttttctt
     3601 ggttgttttt ggaaaagggt gtcgggtggg gctgctgggg cagagctagg gtttgtgttt
     3661 cggctcctcg ctcttggcta tttctataca catatacata tatatatata tatatttaaa
     3721 gaacgatgtg gttagctctg tcccgggttg ttttattact acttagcagc agctccagtt
     3781 ccacacaagg gctcagggta catgcgtcag cctgcactgg ccagctgtgc ccgctgcccg
     3841 gaagcactgc ccagcacttc tctgctgtac caggccaaga gtgatcttgg tttggtgtct
     3901 gatagagaag ggggagaagg ccctgagctg gcaccctact acttacacac aggatggcag
     3961 tggtacctcg agttggtttt ttttttttta aattaaaata aaaatgttta aaaaaaatta
     4021 aagccagaaa tggagttagc agagtgctct gcagtgggtt cctcttggag gtggcaaggg
     4081 caaggggccc acaccactgc ctgctgccca ccaaagccca cctcacttcc ctcatctcca
     4141 gcttttgtct gtgttccgtt ccgtctggct gagccagtcc ctcccagctg gcccaaggcg
     4201 cccagcgtag cagcgggagg ggctgggggc aggtttcatg catgcctttg ctcttcccct
     4261 catgtacaga ttttaatgct tctattacat tcaaccacca caggctggga tttctataca
     4321 tagaacaaaa tcaaacacct gtaataacag caaagccacg cagtacaaat gggaaggggc
     4381 atacctacgg tgtcaggata ctgaaagcaa tacctctcgt tagccttcct cttttgtact
     4441 ttggacacat gcggattcgc agacatcaca aaaatggaca agccccatct ggtgtaaccc
     4501 ctgtcaaata actgtgagca acttcagttc tggaacaata aattccttcc gtaaagac
//
