LOCUS       NM_001037349             936 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus biogenesis of lysosomal organelles complex-1,
            subunit 2 (Bloc1s2), mRNA.
ACCESSION   NM_001037349 XM_215245
VERSION     NM_001037349.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Pu,J., Schindler,C., Jia,R., Jarnik,M., Backlund,P. and
            Bonifacino,J.S.
  TITLE     BORC, a multisubunit complex that regulates lysosome positioning
  JOURNAL   Dev Cell 33 (2), 176-188 (2015)
   PUBMED   25898167
REFERENCE   2  (bases 1 to 936)
  AUTHORS   Lee,H.H., Nemecek,D., Schindler,C., Smith,W.J., Ghirlando,R.,
            Steven,A.C., Bonifacino,J.S. and Hurley,J.H.
  TITLE     Assembly and architecture of biogenesis of lysosome-related
            organelles complex-1 (BLOC-1)
  JOURNAL   J Biol Chem 287 (8), 5882-5890 (2012)
   PUBMED   22203680
REFERENCE   3  (bases 1 to 936)
  AUTHORS   Larimore,J., Tornieri,K., Ryder,P.V., Gokhale,A., Zlatic,S.A.,
            Craige,B., Lee,J.D., Talbot,K., Pare,J.F., Smith,Y. and Faundez,V.
  TITLE     The schizophrenia susceptibility factor dysbindin and its
            associated complex sort cargoes from cell bodies to the synapse
  JOURNAL   Mol Biol Cell 22 (24), 4854-4867 (2011)
   PUBMED   21998198
REFERENCE   4  (bases 1 to 936)
  AUTHORS   Monfregola,J., Napolitano,G., D'Urso,M., Lappalainen,P. and
            Ursini,M.V.
  TITLE     Functional characterization of Wiskott-Aldrich syndrome protein and
            scar homolog (WASH), a bi-modular nucleation-promoting factor able
            to interact with biogenesis of lysosome-related organelle subunit 2
            (BLOS2) and gamma-tubulin
  JOURNAL   J Biol Chem 285 (22), 16951-16957 (2010)
   PUBMED   20308062
  REMARK    GeneRIF: WASH is a bimodular protein and a component of the BLOC-1
            complex in which the C terminus is involved in Arp2/3-mediated
            actin nucleation, whereas the N-terminal portion is required for
            its regulation and localization in the cells
REFERENCE   5  (bases 1 to 936)
  AUTHORS   Sun,J., Nie,J., Hao,B., Li,L., Xing,G., Wang,Z., Zhou,Y., Sun,Q.,
            Li,G., Zhang,L. and He,F.
  TITLE     Ceap/BLOS2 interacts with BRD7 and selectively inhibits its
            transcription-suppressing effect on cellular
            proliferation-associated genes
  JOURNAL   Cell Signal 20 (6), 1151-1158 (2008)
   PUBMED   18329849
REFERENCE   6  (bases 1 to 936)
  AUTHORS   Felten,A., Leister,P., Burgdorf,S., Uhlmann,L. and Scheidtmann,K.H.
  TITLE     Characterization of rat BLOS2/Ceap, a putative yeast She3 homolog,
            as interaction partner of apoptosis antagonizing transcription
            factor/Che-1
  JOURNAL   Biol Chem 388 (6), 569-582 (2007)
   PUBMED   17552904
  REMARK    GeneRIF: In search of novel interaction partners of AATF, we
            identified BLOS2 (BLOC1S2, also termed Ceap) from a rat cDNA
            library; BLOS2 is extremely conserved with a high degree of
            homology to yeast She3p.
REFERENCE   7  (bases 1 to 936)
  AUTHORS   Wang,X., Zhang,Y., Kong,L., Xie,Z., Lin,Z., Guo,N., Strong,J.A.,
            Meij,J.T., Zhao,Z., Jing,N. and Yu,L.
  TITLE     RSEP1 is a novel gene with functional involvement in neuropathic
            pain behaviour
  JOURNAL   Eur J Neurosci 22 (5), 1090-1096 (2005)
   PUBMED   16176350
  REMARK    GeneRIF: rat spinal cord expression protein 1 is a novel gene with
            functional involvement in neuropathic pain behaviour.
REFERENCE   8  (bases 1 to 936)
  AUTHORS   Wang,Z., Wei,H., Yu,Y., Sun,J., Yang,Y., Xing,G., Wu,S., Zhou,Y.,
            Zhu,Y., Zhang,C., Zhou,T., Zhao,X., Sun,Q. and He,F.
  TITLE     Characterization of Ceap-11 and Ceap-16, two novel
            splicing-variant-proteins, associated with centrosome, microtubule
            aggregation and cell proliferation
  JOURNAL   J Mol Biol 343 (1), 71-82 (2004)
   PUBMED   15381421
REFERENCE   9  (bases 1 to 936)
  AUTHORS   Starcevic,M. and Dell'Angelica,E.C.
  TITLE     Identification of snapin and three novel proteins (BLOS1, BLOS2,
            and BLOS3/reduced pigmentation) as subunits of biogenesis of
            lysosome-related organelles complex-1 (BLOC-1)
  JOURNAL   J Biol Chem 279 (27), 28393-28401 (2004)
   PUBMED   15102850
REFERENCE   10 (bases 1 to 936)
  AUTHORS   Vitt,U., Gietzen,D., Stevens,K., Wingrove,J., Becha,S., Bulloch,S.,
            Burrill,J., Chawla,N., Chien,J., Crawford,M., Ison,C., Kearney,L.,
            Kwong,M., Park,J., Policky,J., Weiler,M., White,R., Xu,Y.,
            Daniels,S., Jacob,H., Jensen-Seaman,M.I., Lazar,J., Stuve,L. and
            Schmidt,J.
  TITLE     Identification of candidate disease genes by EST alignments,
            synteny, and expression and verification of Ensembl genes on rat
            chromosome 1q43-54
  JOURNAL   Genome Res 14 (4), 640-650 (2004)
   PUBMED   15060005
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK220925.1 and AY786315.1.
            
            On Oct 17, 2007 this sequence version replaced NM_001037349.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY786315.1, EV770148.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                CK220925.1         1-45
            46-936              AY786315.1         27-917
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q54"
     gene            1..936
                     /gene="Bloc1s2"
                     /gene_synonym="RSEP1; Sep1"
                     /note="biogenesis of lysosomal organelles complex-1,
                     subunit 2"
                     /db_xref="GeneID:293938"
                     /db_xref="RGD:1306551"
     exon            1..77
                     /gene="Bloc1s2"
                     /gene_synonym="RSEP1; Sep1"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..451
                     /gene="Bloc1s2"
                     /gene_synonym="RSEP1; Sep1"
                     /note="spinal cord-expressed protein 1; BLOC-1 subunit 2"
                     /codon_start=1
                     /product="biogenesis of lysosome-related organelles
                     complex-1 subunit 2"
                     /protein_id="NP_001032426.1"
                     /db_xref="GeneID:293938"
                     /db_xref="RGD:1306551"
                     /translation="MAAAAEGVPATRREEPPRDDAAVETAEEAKEPAEADINELCRDM
                     FSKMATYLTGELTATSEDYKLLENMNKLTSLKYLEMKDIAINISRNLKDLNQKYAGLQ
                     PYLDQINVIEEQVAALEQAAYKLDAYSKKLEAKYKKLEKR"
     exon            78..194
                     /gene="Bloc1s2"
                     /gene_synonym="RSEP1; Sep1"
                     /inference="alignment:Splign:2.1.0"
     exon            195..314
                     /gene="Bloc1s2"
                     /gene_synonym="RSEP1; Sep1"
                     /inference="alignment:Splign:2.1.0"
     exon            315..419
                     /gene="Bloc1s2"
                     /gene_synonym="RSEP1; Sep1"
                     /inference="alignment:Splign:2.1.0"
     exon            420..883
                     /gene="Bloc1s2"
                     /gene_synonym="RSEP1; Sep1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggaagcagc cttgggtcgg gcatggcggc ggctgcggag ggcgtcccgg cgacgcgacg
       61 ggaggagccg cctcgagacg atgctgcggt ggagacagct gaggaagcaa aggagcctgc
      121 agaagctgac atcaatgaac tctgccggga catgttctcc aaaatggcca catatttgac
      181 tggggaactg acagccacca gtgaagacta caaactcctg gaaaatatga ataagctcac
      241 gagcttgaag taccttgaaa tgaaagacat tgctataaac atcagcagaa acctgaagga
      301 cttaaaccag aaatatgccg gactgcagcc atatctggat cagatcaacg tgattgagga
      361 gcaggtggca gctctcgagc aggcagccta caagttggat gcttattcaa agaaactgga
      421 agccaagtac aagaagttgg agaagcgatg agaatcatgt tcgtgtgtct caagtgtttt
      481 ttaatatgga agttgtttta taaccactaa atcctgaggc tgacaattat cacagttcct
      541 cagaagaaaa ctgctggtta atcccaagac cacacgatac cacggatgaa ctggagggct
      601 gcggctgttc tggagtctcc tctgaggtgg tacctctcag aaactcacac ccagaatgcc
      661 tcaccgttta cttgaatgct tcatcatcca tacaagatgg agactctaga gttcagggca
      721 tacctgggct tgccagtaga gtccagggag gggacctgct ttccctggta ttgggcaatt
      781 agcacgttat tttctaaaat ggctgttttg ttcgttttgt tttctactga tgatataatg
      841 tattattaac aagattctaa attaaaaggg aaaacaaaac ataaaaaaaa aaaaaaaaaa
      901 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//
