LOCUS       NM_030850               1884 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Rattus norvegicus betaine-homocysteine S-methyltransferase (Bhmt),
            mRNA.
ACCESSION   NM_030850
VERSION     NM_030850.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Zhang,W.H., Zhao,S.M., Guo,J.F., Liu,Y.P. and Jiang,Y.Q.
  TITLE     BHMT polymorphism and susceptibility to PTE in Chinese patients
  JOURNAL   Eur Rev Med Pharmacol Sci 27 (9), 4098-4102 (2023)
   PUBMED   37203835
  REMARK    Erratum:[Eur Rev Med Pharmacol Sci. 2023 Jul;27(13):5947. doi:
            10.26355/eurrev_202307_32944.. PMID: 37458620]
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Cao,Y., Ding,W., Zhang,J., Gao,Q., Yang,H., Cao,W., Wang,Z.,
            Fang,L. and Du,R.
  TITLE     Significant Down-Regulation of Urea Cycle Generates Clinically
            Relevant Proteomic Signature in Hepatocellular Carcinoma Patients
            with Macrovascular Invasion
  JOURNAL   J Proteome Res 18 (5), 2032-2044 (2019)
   PUBMED   30901224
REFERENCE   3  (bases 1 to 1884)
  AUTHORS   Garrido,F., Pacheco,M., Vargas-Martinez,R., Velasco-Garcia,R.,
            Jorge,I., Serrano,H., Portillo,F., Vazquez,J. and Pajares,M.A.
  TITLE     Identification of hepatic protein-protein interaction targets for
            betaine homocysteine S-methyltransferase
  JOURNAL   PLoS One 13 (6), e0199472 (2018)
   PUBMED   29924862
  REMARK    GeneRIF: the array of interactions of betaine homocysteine
            S-methyltransferase in normal liver of Rattus norvegicus, were
            examined.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1884)
  AUTHORS   Perez-Miguelsanz,J., Vallecillo,N., Garrido,F., Reytor,E.,
            Perez-Sala,D. and Pajares,M.A.
  TITLE     Betaine homocysteine S-methyltransferase emerges as a new player of
            the nuclear methionine cycle
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1864 (7), 1165-1182 (2017)
   PUBMED   28288879
REFERENCE   5  (bases 1 to 1884)
  AUTHORS   Carter,W.G., Vigneswara,V., Newlaczyl,A., Wayne,D., Ahmed,B.,
            Saddington,S., Brewer,C., Raut,N., Gerdes,H.K., Erdozain,A.M.,
            Tooth,D., Bolt,E.L., Osna,N.A., Tuma,D.J. and Kharbanda,K.K.
  TITLE     Isoaspartate, carbamoyl phosphate synthase-1, and carbonic
            anhydrase-III as biomarkers of liver injury
  JOURNAL   Biochem Biophys Res Commun 458 (3), 626-631 (2015)
   PUBMED   25684186
REFERENCE   6  (bases 1 to 1884)
  AUTHORS   Gonzalez,B., Pajares,M.A., Martinez-Ripoll,M., Blundell,T.L. and
            Sanz-Aparicio,J.
  TITLE     Crystal structure of rat liver betaine homocysteine
            s-methyltransferase reveals new oligomerization features and
            conformational changes upon substrate binding
  JOURNAL   J Mol Biol 338 (4), 771-782 (2004)
   PUBMED   15099744
  REMARK    GeneRIF: Data report the crystal structure of rat liver betaine
            homocysteine s-methyltransferase at 2.5A resolution.
REFERENCE   7  (bases 1 to 1884)
  AUTHORS   Forestier,M., Banninger,R., Reichen,J. and Solioz,M.
  TITLE     Betaine homocysteine methyltransferase: gene cloning and expression
            analysis in rat liver cirrhosis
  JOURNAL   Biochim Biophys Acta 1638 (1), 29-34 (2003)
   PUBMED   12757931
  REMARK    GeneRIF: gene cloning and expression analysis in liver cirrhosis
REFERENCE   8  (bases 1 to 1884)
  AUTHORS   Gonzalez,B., Campillo,N., Garrido,F., Gasset,M., Sanz-Aparicio,J.
            and Pajares,M.A.
  TITLE     Active-site-mutagenesis study of rat liver betaine-homocysteine
            S-methyltransferase
  JOURNAL   Biochem J 370 (Pt 3), 945-952 (2003)
   PUBMED   12487625
  REMARK    GeneRIF: Results describe the site-directed-mutagenesis of putative
            active-site residues in rat liver betaine-homocysteine
            S-methyltransferase.
REFERENCE   9  (bases 1 to 1884)
  AUTHORS   Sowden,M.P., Collins,H.L., Smith,H.C., Garrow,T.A., Sparks,J.D. and
            Sparks,C.E.
  TITLE     Apolipoprotein B mRNA and lipoprotein secretion are increased in
            McArdle RH-7777 cells by expression of betaine-homocysteine
            S-methyltransferase
  JOURNAL   Biochem J 341 (Pt 3) (Pt 3), 639-645 (1999)
   PUBMED   10417327
REFERENCE   10 (bases 1 to 1884)
  AUTHORS   Forestier,M., Reichen,J. and Solioz,M.
  TITLE     Application of mRNA differential display to liver cirrhosis:
            reduced fetuin expression in biliary cirrhosis in the rat
  JOURNAL   Biochem Biophys Res Commun 225 (2), 377-383 (1996)
   PUBMED   8753772
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF038870.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF038870.1, BC078811.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q12"
     gene            1..1884
                     /gene="Bhmt"
                     /note="betaine-homocysteine S-methyltransferase"
                     /db_xref="GeneID:81508"
                     /db_xref="RGD:621496"
     exon            1..103
                     /gene="Bhmt"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..1294
                     /gene="Bhmt"
                     /EC_number="2.1.1.5"
                     /function="converts betaine to dimethylglycine and
                     homocysteine to methionine"
                     /note="betaine-homocysteine methyltransferase"
                     /codon_start=1
                     /product="betaine--homocysteine S-methyltransferase 1"
                     /protein_id="NP_110477.1"
                     /db_xref="GeneID:81508"
                     /db_xref="RGD:621496"
                     /translation="MAPIAGKKAKRGILERLNAGEVVIGDGGFVFALEKRGYVKAGPW
                     TPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVAEKISGQKVNEA
                     ACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKIFHQQLEVFMKKNVDFLIAEY
                     FEHVEEAVWAVEALKTSGKPIAATMCIGPEGDLHGVSPGECAVRLVKAGAAIVGVNCH
                     FDPSTSLQTIKLMKEGLEAARLKAYLMSHALAYHTPDCGKQGFIDLPEFPFGLEPRVA
                     TRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSG
                     LDMHTKPWIRARARKEYWQNLRIASGRPYNPSMSKPDAWGVTKGAAELMQQKEATTEQ
                     QLRALFEKQKFKSAQ"
     exon            104..236
                     /gene="Bhmt"
                     /inference="alignment:Splign:2.1.0"
     exon            237..355
                     /gene="Bhmt"
                     /inference="alignment:Splign:2.1.0"
     exon            356..547
                     /gene="Bhmt"
                     /inference="alignment:Splign:2.1.0"
     exon            548..695
                     /gene="Bhmt"
                     /inference="alignment:Splign:2.1.0"
     exon            696..878
                     /gene="Bhmt"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1107
                     /gene="Bhmt"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1866
                     /gene="Bhmt"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1864
                     /gene="Bhmt"
ORIGIN      
        1 caagcctttg ctggagaccg ctcctgtcca gtccgcagct ggcttcagcg ccactcagga
       61 caccggaaag atggcaccga ttgccggcaa gaaggccaag aggggaatct tagaacgctt
      121 aaatgctggc gaagtcgtga tcggagatgg gggatttgtc tttgcactgg aaaagagggg
      181 ctacgtaaag gctggaccct ggaccccaga ggctgcggtg gagcaccccg aggcagttcg
      241 gcagcttcat cgggagttcc tcagagctgg atcgaacgtc atgcagacct tcactttcta
      301 tgcaagtgag gacaagctgg aaaaccgagg gaactacgtg gcagagaaga tatctgggca
      361 gaaggtcaat gaagctgctt gtgacattgc acggcaagtt gctgacgaag gggatgcatt
      421 ggttgcagga ggtgtgagtc agacaccttc ctacctcagc tgcaagagtg agacggaagt
      481 taaaaagata tttcaccaac agcttgaggt cttcatgaag aagaatgtgg acttcctcat
      541 tgcagagtat tttgaacatg ttgaagaagc cgtgtgggca gtcgaggcct taaaaacatc
      601 cgggaagcct atagcggcta ccatgtgcat cggacctgaa ggagatctac atggcgtgtc
      661 tcctggagag tgcgcagtgc gtttggtaaa agcaggtgcc gccattgtcg gtgtgaactg
      721 ccacttcgac cccagcacca gcttgcagac aataaagctc atgaaggagg gtctggaagc
      781 agctcggctg aaggcttact tgatgagcca cgccctggcc taccacaccc ctgactgtgg
      841 caaacaggga tttattgatc tcccagaatt cccctttgga ttggaaccca gagttgccac
      901 cagatgggat attcaaaaat acgccagaga ggcctacaac ctgggggtca ggtacattgg
      961 cggctgctgc ggatttgagc cctaccacat cagggccatt gcagaggagc tcgccccaga
     1021 aaggggattt ttaccaccag cttcagaaaa acatggcagc tggggaagtg gtttggacat
     1081 gcacaccaaa ccctggatca gggcaagggc caggaaagaa tactggcaga atcttcgaat
     1141 agcttcgggc agaccgtaca atccttcgat gtccaagccg gatgcttggg gagtgacgaa
     1201 aggggcagca gagctgatgc agcagaagga agccaccact gagcagcagc tgagagcgct
     1261 cttcgaaaaa caaaaattca aatccgcaca gtagccacag gccagcggtt cggggcgaat
     1321 tcctccaggt ccgggccaca gtgtgcaccc ggaaggagaa ggcatctcta aaccagcgtt
     1381 tgtgttgatg ccggcttaca cctgtgattg gtgctagtta gacaaaatgg agtcacagat
     1441 agcatttcac agttacaaaa ctacgcttta gaattttacc tagaaggaag aaaggagaag
     1501 tccacagtaa atcctgaaca catttcctac gtgcctgtcg cattacaggc gcacaggagt
     1561 cactgcagcg aagagaaagt cacccgacgt caatctcatt tcagataggg ggataggaca
     1621 ccacctccac gagtgacata gaaccattca gggaccgtat cataagtgac acagcaacca
     1681 tctatatcta agatgcttcc caagtggatt ccaagatctt ttgagcagga cccttaggca
     1741 gaaacaacac acaccagccc tgtaaaactt aacagataac tgatccattc tgtaattctg
     1801 taatctctgt tctgactgct tccattccat ttcattaata aaaacatgcc ggttgaaaac
     1861 cttcaaaaaa aaaaaaaaaa aaaa
//
