LOCUS       NM_001191783            2970 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus bestrophin 3 (Best3), mRNA.
ACCESSION   NM_001191783 XM_235161
VERSION     NM_001191783.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Jiang,L., Liu,Y., Ma,M.M., Tang,Y.B., Zhou,J.G. and Guan,Y.Y.
  TITLE     Mitochondria dependent pathway is involved in the protective effect
            of bestrophin-3 on hydrogen peroxide-induced apoptosis in basilar
            artery smooth muscle cells
  JOURNAL   Apoptosis 18 (5), 556-565 (2013)
   PUBMED   23468120
  REMARK    GeneRIF: Best-3 inhibits apoptosis induced by H2O2 in basilar
            artery smooth muscle cells through mitochondria dependent pathway.
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Lee,W.K., Chakraborty,P.K., Roussa,E., Wolff,N.A. and Thevenod,F.
  TITLE     ERK1/2-dependent bestrophin-3 expression prevents ER-stress-induced
            cell death in renal epithelial cells by reducing CHOP
  JOURNAL   Biochim Biophys Acta 1823 (10), 1864-1876 (2012)
   PUBMED   22705154
  REMARK    GeneRIF: ERK1/2-dependent Best-3 activation regulates cell fate
            decisions during endoplasmic reticulum stress by suppressing CHOP
            induction and death.
REFERENCE   3  (bases 1 to 2970)
  AUTHORS   Broegger,T., Jacobsen,J.C., Secher Dam,V., Boedtkjer,D.M.,
            Kold-Petersen,H., Pedersen,F.S., Aalkjaer,C. and Matchkov,V.V.
  TITLE     Bestrophin is important for the rhythmic but not the tonic
            contraction in rat mesenteric small arteries
  JOURNAL   Cardiovasc Res 91 (4), 685-693 (2011)
   PUBMED   21498420
  REMARK    GeneRIF: Bestrophin-3 expression was significantly reduced compared
            with arteries transfected with mutated siRNA
REFERENCE   4  (bases 1 to 2970)
  AUTHORS   Matchkov,V.V., Larsen,P., Bouzinova,E.V., Rojek,A., Boedtkjer,D.M.,
            Golubinskaya,V., Pedersen,F.S., Aalkjaer,C. and Nilsson,H.
  TITLE     Bestrophin-3 (vitelliform macular dystrophy 2-like 3 protein) is
            essential for the cGMP-dependent calcium-activated chloride
            conductance in vascular smooth muscle cells
  JOURNAL   Circ Res 103 (8), 864-872 (2008)
   PUBMED   18776041
  REMARK    GeneRIF: Bestrophin-3 (vitelliform macular dystrophy 2-like 3
            protein) is essential for the cGMP-dependent calcium-activated
            chloride conductance in vascular smooth muscle cells.
REFERENCE   5  (bases 1 to 2970)
  AUTHORS   Qu,Z., Cui,Y. and Hartzell,C.
  TITLE     A short motif in the C-terminus of mouse bestrophin 3 [corrected]
            inhibits its activation as a Cl channel
  JOURNAL   FEBS Lett 580 (8), 2141-2146 (2006)
   PUBMED   16563389
  REMARK    Erratum:[FEBS Lett. 2007 Feb 6;581(3):580]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Jul 17, 2010 this sequence version replaced XM_235161.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643296.19585.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760475
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-152               JAXUCZ010000007.1  54501717-54501868
            153-247             JAXUCZ010000007.1  54505464-54505558
            248-481             JAXUCZ010000007.1  54506015-54506248
            482-636             JAXUCZ010000007.1  54519373-54519527
            637-714             JAXUCZ010000007.1  54520854-54520931
            715-867             JAXUCZ010000007.1  54521369-54521521
            868-948             JAXUCZ010000007.1  54524839-54524919
            949-1100            JAXUCZ010000007.1  54526140-54526291
            1101-2970           JAXUCZ010000007.1  54540871-54542740
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q22"
     gene            1..2970
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /note="bestrophin 3"
                     /db_xref="GeneID:314847"
                     /db_xref="RGD:1305439"
     CDS             1..2019
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /note="vitelliform macular dystrophy 2-like 3"
                     /codon_start=1
                     /product="bestrophin-3"
                     /protein_id="NP_001178712.1"
                     /db_xref="GeneID:314847"
                     /db_xref="RGD:1305439"
                     /translation="MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYT
                     AISLVYRLLLTGAQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPW
                     PDRLMFLISSSVHGSDQHGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEA
                     GFMTADERKLFDHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYR
                     SWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIF
                     TLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKK
                     DIYWDDSAARPPYTLAAADYCIPSFLGSTIQMGLSGSDFPAEDWLWNYEKQGHRHSVM
                     RRVKRFLSTHEHPGSPKRRRSFGRQASDSSMFFPPSPARDLLNVPSRNPHRGSPTRKQ
                     SHSQEGSPKLHSSMGELSTIRETSRTSTLQSLSQQSSVRASPTKMPQVPEVLITAAEA
                     PEAAFASDNHQRDSTTSILSLEFTGVQASGTEQQEDPSGKVTPPGDPEPQTISAGTEK
                     DLFKFEEDLDDDIFPKRWSLPVFRESRHTSLGNLGPDPVSPENALLLDTETSSETNGV
                     HPGAGSAVSPDILYLMESLDKETDILEFNNEHTEEPLKGTPPRPRTWF"
     exon            1..152
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            153..247
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            248..481
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            482..636
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            637..714
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            715..867
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            868..948
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1100
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..2970
                     /gene="Best3"
                     /gene_synonym="Vmd2l3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgactgtca cttactccag taaagtagca aatgcgactt tttttggatt tcataggttg
       61 ctcctcaagt ggagaggcag catctacaaa ctgctgtaca gggaatttat tgtttttgct
      121 gttctttaca cagcaatcag tctggtgtac agattgttac ttacaggagc ccaaaaacgt
      181 tactttgaaa aattatcaat ttactgtgac agatatgctg aacaaattcc agtaaccttt
      241 gtgcttgggt tttacgttac tctggtagtg aaccgatggt ggaaccagtt tgtgaatctg
      301 ccttggccag acaggctgat gttcctcatc tccagcagtg tccacgggag cgaccagcat
      361 gggcgcctgc tcagaaggac gctgatgcgc tacgtcaatc tcacgtcctt gctcatcttc
      421 cgctcggtga gcacagcggt gtacaaaaga ttccccacca tggaccacgt ggtcgaagca
      481 ggttttatga cggcagatga gaggaagtta ttcgaccacc tcaagtcgcc tcatctgaaa
      541 tactgggttc cgttcatttg gtttggaaat cttgcaacca aagcccggaa tgaaggcaga
      601 atcagagaca gcgtcgatct acaatcactg atgaccgaaa tgaatcggta ccgctcttgg
      661 tgcagcctct tatttggtta cgactgggtt ggcattccac tggtttacac acaggtcgtc
      721 actctcgccg tctacacgtt cttctttgca tgcctgatcg gacggcagtt cctggatccc
      781 accaaaggct acgcgggaca cgacttggac ctgtacattc ccatcttcac cctcctccag
      841 ttcttcttct acgcaggatg gctcaaggtt gccgagcagc tgatcaaccc tttcggggaa
      901 gacgacgacg acttcgaaac aaactggtgc attgacagaa acctgcaggt ctctcttctg
      961 gctgtggatg aaatgcacat gagtttaccc aagatgaaga aggacattta ctgggacgac
     1021 tctgctgctc ggccaccgta cacgctggca gcggctgact actgtatacc ttccttcctg
     1081 ggctcaacaa tccaaatggg gctgtccggg tccgactttc ccgcggagga ttggctgtgg
     1141 aactatgaga agcaaggcca ccggcactcg gtgatgagga gggtcaagcg tttcctgagt
     1201 acccacgagc accccggcag tcccaagagg aggaggagtt tcggcaggca ggccagtgac
     1261 agctccatgt tcttcccccc aagcccagct cgggatctgc tgaatgtgcc ctcaaggaac
     1321 ccccaccgag gctcacctac ccggaagcaa tcccactccc aggagggcag ccctaagctt
     1381 cactccagca tgggagaatt gtccaccatc agggagacca gccgcaccag cactctgcag
     1441 agcctgagcc agcagtccag tgtgagggcc tcccctacca aaatgcccca ggtccccgag
     1501 gtcctaatca ctgcagccga agccccggag gcggcgttcg catcagacaa ccatcagcgt
     1561 gactccacta cctccatctt gagcctcgag ttcactgggg tgcaggcgag cgggactgag
     1621 cagcaggaag acccctcggg gaaggtgaca cctcctgggg accccgaacc ccagactatt
     1681 tcagccggga ctgaaaaaga cttgttcaag tttgaggaag atctagatga tgacatattc
     1741 cccaaaaggt ggagcctacc agtgtttcgg gagtccaggc acacctccct aggaaactta
     1801 ggtccggatc ctgtgagtcc agagaatgct cttttacttg acacagaaac atcctcagag
     1861 accaacggag tccatcctgg ggctggctct gccgtctctc ccgacatcct gtatttaatg
     1921 gaaagtttgg ataaggaaac ggacatcctg gagtttaaca acgagcacac tgaagaaccc
     1981 cttaagggaa caccgccgcg ccccaggacc tggttctagc ctagcttcct actttatata
     2041 aagctgtgcg cactgaccca gggggaacag cccccaggaa ctggccagca tttagagaaa
     2101 tgatcctact atacaggcta cttcgaactc cttagggcat tatgttgctg caaagctcaa
     2161 acgcatccac atcgtctagc acaaaggggc tttcgtgaaa attatacagc ccaaagtgga
     2221 ttactgtaac caaatgaggg acagcataca aatgtataca cctacatctg attagctttg
     2281 gagagagcca taacagatca tagtccaatc ccatagaaag cccagaaaca aagggccctg
     2341 ttccttttcc ggctccttcc tgtaccgctc tttttttttt tttcctttag ctacagagac
     2401 aagaaatagg ccataccatg cccagaggcc tttttcttat agttgttcca ctgacttcct
     2461 gaccatctca cacccacctt ctataactgg tgtttctgta actcttaaat tccctcagca
     2521 ccagaaagtt ccttttctct tcaagaacga tctcactgtc tgatcttcat ttttctctct
     2581 ccctctttac tttcttcctc catttttcct cgtccctcct ccctcgtcta gctcctccca
     2641 tgactctaat ctgtccccct ttccaggtta caacattttt ctctttccaa ctcggttgct
     2701 cattgcttgt cacatgtgca aactctggga tactgggagg ggagactgac acacccagcc
     2761 tttcatgctt ccagccctgc tgtaagaggt gtatgtgggt tgatggtgtg tatgtgcgct
     2821 gacggtgtcc gccactatcg caaacactcc gttcctgttc cttcacctta gaaaccggag
     2881 aagaaggagc tggggattta gctcagtggt agagcgctta cctaggaagc gcaaggccct
     2941 gggttcggtc cccagctccg gggaaaaaaa
//
