LOCUS       NM_001127712            2537 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus beta-carotene oxygenase 2 (Bco2), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_001127712 XM_001070555 XM_236220
VERSION     NM_001127712.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Widjaja-Adhi MA, Lobo GP, Golczak M and Von Lintig J.
  TITLE     A genetic dissection of intestinal fat-soluble vitamin and
            carotenoid absorption
  JOURNAL   Hum Mol Genet 24 (11), 3206-3219 (2015)
   PUBMED   25701869
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Raghuvanshi S, Reed V, Blaner WS and Harrison EH.
  TITLE     Cellular localization of beta-carotene 15,15' oxygenase-1 (BCO1)
            and beta-carotene 9',10' oxygenase-2 (BCO2) in rat liver and
            intestine
  JOURNAL   Arch Biochem Biophys 572, 19-27 (2015)
   PUBMED   25575786
  REMARK    GeneRIF: Cellular localization of beta-carotene 15,15' oxygenase-1
            (BCO1) and beta-carotene 9',10' oxygenase-2 (BCO2) in rat liver and
            intestine
REFERENCE   3  (bases 1 to 2537)
  AUTHORS   Kim YS, Yeom SJ and Oh DK.
  TITLE     Production of beta-apo-10'-carotenal from beta-carotene by human
            beta-carotene-9',10'-oxygenase expressed in E. coli
  JOURNAL   Biotechnol Lett 33 (6), 1195-1200 (2011)
   PUBMED   21302131
REFERENCE   4  (bases 1 to 2537)
  AUTHORS   Amengual J, Lobo GP, Golczak M, Li HN, Klimova T, Hoppel CL, Wyss
            A, Palczewski K and von Lintig J.
  TITLE     A mitochondrial enzyme degrades carotenoids and protects against
            oxidative stress
  JOURNAL   FASEB J 25 (3), 948-959 (2011)
   PUBMED   21106934
REFERENCE   5  (bases 1 to 2537)
  AUTHORS   Kiefer C, Hessel S, Lampert JM, Vogt K, Lederer MO, Breithaupt DE
            and von Lintig J.
  TITLE     Identification and characterization of a mammalian enzyme
            catalyzing the asymmetric oxidative cleavage of provitamin A
  JOURNAL   J Biol Chem 276 (17), 14110-14116 (2001)
   PUBMED   11278918
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC133725.1.
            
            On or before May 31, 2008 this sequence version replaced
            XM_001070555.1, XM_236220.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC133725.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q23"
     gene            1..2537
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /note="beta-carotene oxygenase 2"
                     /db_xref="GeneID:315644"
                     /db_xref="RGD:1309022"
     exon            1..61
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            62..264
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..1699
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /EC_number="1.14.99.-"
                     /note="carotenoid 9',10' monoxygenase II; beta-carotene
                     9', 10'-dioxygenase 2; beta,beta-carotene
                     9',10'-oxygenase; carotenoid 9',10' monooxygenase II"
                     /codon_start=1
                     /product="carotenoid-cleaving dioxygenase, mitochondrial"
                     /protein_id="NP_001121184.1"
                     /db_xref="GeneID:315644"
                     /db_xref="RGD:1309022"
                     /translation="MLGPQQSLPCIAPLLTTVEETLSTVSARVRGHIPEWLNGYLLRV
                     GPGKFEFGKDRYNHWFDGMALLHQFKMEKGTVTYKSKFLQSDTYKANSAGDRIVISEF
                     GTLALPDPCKSIFERFMSRFEPPTMTDNTSVNFVQYKGDYYMSTETNFMNKVDIETLE
                     RTEKVDWSKFVAVNGATAHPHYDPDGTAYNMGNTYGPRGSCYNIIRVPPKKKEPGETI
                     HGAQVVCSIASSEKMKPSYYHSFGMTKNYIVFVEQPLKMKLWKIITSKIRGKSFADGI
                     SWEPQYNTRFHVVDKHTGQPLPGVYYSKPFLTYHQINAFEDQGCIVIDLCCEDDGRSL
                     DIYQLQNLRKAGEELDQVYKAKAKSFPRRFVLPLDISVGAPEGENLRPLPYSSASVVK
                     QGDREIWCSPENLHQEDLEEEGGIEFPQINYGRFSGKKYSFFYGCGFRHLVGDSLIKV
                     DVVNKTLRVWREEGCYPSEPVFVPVPGADEEDSGAILSVVITPNQGESNFLLVLDAKN
                     FTELGRAEVPVRMPYGFHGTFVPI"
     exon            265..476
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            477..592
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            593..695
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            696..824
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            825..985
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1153
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1291
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1474
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1585
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..2513
                     /gene="Bco2"
                     /gene_synonym="Ab2-079; Bcdo2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcaacctt cctgacgctg tgctgcaggg gttctttctg tgacagtgta ccatctccca
       61 gttttgaaga actacgtggg ggaatattca ccggaaaacc atgttgggac cacagcagag
      121 tctgccgtgc attgccccgc tgttgaccac ggtggaggag acgctgagca ctgtctccgc
      181 tcgggtccga ggacacattc ctgaatggct caatggctat ctacttcgag ttggacctgg
      241 gaaatttgag tttggaaagg atagatacaa tcattggttt gatggaatgg ctttgcttca
      301 ccagttcaaa atggagaagg gtacagtgac atacaagagc aagtttctac agagtgatac
      361 atacaaggcc aacagtgcgg gagatagaat cgtgatctca gaatttggca cgctggccct
      421 tcctgaccca tgcaagagca tctttgaacg tttcatgtca aggtttgagc cacctactat
      481 gaccgacaac accagcgtca actttgtgca gtacaaaggt gattactaca tgagcacaga
      541 gaccaatttt atgaataaag tggacattga aacactggaa agaacagaaa aggtggactg
      601 gagcaaattc gttgctgtga atggagcgac cgcacaccct cactatgacc cagacgggac
      661 agcatacaat atggggaaca cctatggacc aagaggttct tgctataata ttattcgtgt
      721 tcctccaaaa aagaaagaac ccggggagac gattcatggg gcgcaggtag tatgttctat
      781 agcttcctct gagaagatga agccttctta ctaccatagc tttggaatga caaaaaacta
      841 catagtcttt gtcgaacagc ctctaaagat gaagctgtgg aaaataatta cttctaaaat
      901 ccggggaaag tccttcgccg atggcataag ctgggaaccc cagtataaca cgcgatttca
      961 cgtggtggat aagcacactg gacagcctct cccaggagtg tactacagca agccttttct
     1021 tacctatcat caaatcaatg ccttcgagga ccagggctgt attgtgattg acctgtgctg
     1081 tgaggatgat gggagaagcc tagacattta ccaactacag aatctcagga aagccggaga
     1141 agagctcgat caggtctata aggcaaaggc aaagtctttc cctcgaagat ttgtcttgcc
     1201 cttggatatc agtgtgggtg cccccgaggg agagaacctc agacccctgc cctattcttc
     1261 agccagtgtc gtgaaacagg gtgacagaga gatctggtgc tctcctgaaa atctacacca
     1321 agaggacctg gaagaggaag ggggaattga atttcctcag atcaactatg gccgattcag
     1381 tggcaaaaag tatagtttct tctatggctg cggttttcga catttggtgg gggattctct
     1441 gattaaggtt gacgtggtga acaagacact aagggtttgg agagaagaag gctgttatcc
     1501 ttcggagccc gtttttgttc cggtgccagg agccgatgag gaagacagtg gggctatact
     1561 ctctgtggtg atcactccca accagggtga aagcaacttt ctccttgtct tggatgccaa
     1621 gaactttaca gaactggggc gagcagaggt acctgtgcgg atgccttatg ggttccacgg
     1681 cacatttgtg cctatctgat ggcaaaagct taaggaagga ctcagataag gcttcaatga
     1741 gcacatttct atggaaaaga gaaaatggtg ggcgtcttag ggttttactg ctgtgaacag
     1801 ataccatgac cagggcacgt cttaaaagga catttaattg aggctggctt acaagttcag
     1861 aggttcagtc cattatcatc gaggtgagag catggtggcg tccaggcagg catggtgcac
     1921 gcagagctga gagttcaaca tcatcatctg aaggctgcta gtggaagact ggcttcttaa
     1981 agccctcaca gtcatacacc tacttcaaca aggccacaac tcctaacagt gccactccct
     2041 ggcccaagca tattcaaacc atgccagtgg gtctcaccca aaagctactg tagtttggcc
     2101 ccgaccctga ccctgaccct gaccctgacc accatgggct catattttac atgattgttc
     2161 ccaggttgtg gcaccacttt gggaggctgt gtaaactttt gggggtgatg ccccacaggc
     2221 accaggagcc agcctttgag gtccctggtt ctctcatgct ctctctctgc ttcctggtcc
     2281 actactatgt ggacagcctt cattccatgt tctcagcctt gtggctgatg gggtttctcc
     2341 tccatgcctc cctactgagt cccctcagtc tgtgggccaa ggatatattt gcaccttaag
     2401 ttgttctgtc agtttgagtt tgcttttggt ttgcagcagt ggtgaaataa ttaataaaaa
     2461 agaaccccga tgtatggctt tacatttcta ttaaaacata atttgtgaga taaaaaaaaa
     2521 aaaaaaaaaa aaaaaaa
//
