LOCUS       NM_022400               1544 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_022400
VERSION     NM_022400.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Dhanani,Z.N., Mann,G. and Adegoke,O.A.J.
  TITLE     Depletion of branched-chain aminotransferase 2 (BCAT2) enzyme
            impairs myoblast survival and myotube formation
  JOURNAL   Physiol Rep 7 (23), e14299 (2019)
   PUBMED   31833233
  REMARK    GeneRIF: Depletion of branched-chain aminotransferase 2 (BCAT2)
            enzyme impairs myoblast survival and myotube formation.
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Moghei,M., Tavajohi-Fini,P., Beatty,B. and Adegoke,O.A.
  TITLE     Ketoisocaproic acid, a metabolite of leucine, suppresses
            insulin-stimulated glucose transport in skeletal muscle cells in a
            BCAT2-dependent manner
  JOURNAL   Am J Physiol Cell Physiol 311 (3), C518-C527 (2016)
   PUBMED   27488662
  REMARK    GeneRIF: The effect of KIC on insulin-stimulated glucose transport
            was abrogated in cells depleted of branched-chain aminotransferase
            2 (BCAT2).
REFERENCE   3  (bases 1 to 1544)
  AUTHORS   Tarantino,C., Paolella,G., Cozzuto,L., Minopoli,G., Pastore,L.,
            Parisi,S. and Russo,T.
  TITLE     miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic
            stem cells
  JOURNAL   FASEB J 24 (9), 3255-3263 (2010)
   PUBMED   20439489
REFERENCE   4  (bases 1 to 1544)
  AUTHORS   Lang,C.H., Lynch,C.J. and Vary,T.C.
  TITLE     Alcohol-induced IGF-I resistance is ameliorated in mice deficient
            for mitochondrial branched-chain aminotransferase
  JOURNAL   J Nutr 140 (5), 932-938 (2010)
   PUBMED   20237068
REFERENCE   5  (bases 1 to 1544)
  AUTHORS   Islam,M.M., Nautiyal,M., Wynn,R.M., Mobley,J.A., Chuang,D.T. and
            Hutson,S.M.
  TITLE     Branched-chain amino acid metabolon: interaction of glutamate
            dehydrogenase with the mitochondrial branched-chain
            aminotransferase (BCATm)
  JOURNAL   J Biol Chem 285 (1), 265-276 (2010)
   PUBMED   19858196
  REMARK    GeneRIF: GDH1 facilitates regeneration of BCATm that binds to E1
            decarboxylase of the branched-chain alpha-keto acid dehydrogenase
            enzyme complex, promotes metabolon formation, branched-chain amino
            acid oxidation, and cycling of nitrogen through glutamate.
REFERENCE   6  (bases 1 to 1544)
  AUTHORS   Yennawar,N., Dunbar,J., Conway,M., Hutson,S. and Farber,G.
  TITLE     The structure of human mitochondrial branched-chain
            aminotransferase
  JOURNAL   Acta Crystallogr D Biol Crystallogr 57 (Pt 4), 506-515 (2001)
   PUBMED   11264579
REFERENCE   7  (bases 1 to 1544)
  AUTHORS   Tovar,A.R., Becerril,E., Hernandez-Pando,R., Lopez,G., Suryawan,A.,
            Desantiago,S., Hutson,S.M. and Torres,N.
  TITLE     Localization and expression of BCAT during pregnancy and lactation
            in the rat mammary gland
  JOURNAL   Am J Physiol Endocrinol Metab 280 (3), E480-E488 (2001)
   PUBMED   11171603
REFERENCE   8  (bases 1 to 1544)
  AUTHORS   Bledsoe,R.K., Dawson,P.A. and Hutson,S.M.
  TITLE     Cloning of the rat and human mitochondrial branched chain
            aminotransferases (BCATm)
  JOURNAL   Biochim Biophys Acta 1339 (1), 9-13 (1997)
   PUBMED   9165094
REFERENCE   9  (bases 1 to 1544)
  AUTHORS   Hutson,S.M. and Hall,T.R.
  TITLE     Identification of the mitochondrial branched chain aminotransferase
            as a branched chain alpha-keto acid transport protein
  JOURNAL   J Biol Chem 268 (5), 3084-3091 (1993)
   PUBMED   8428987
REFERENCE   10 (bases 1 to 1544)
  AUTHORS   Hall,T.R., Wallin,R., Reinhart,G.D. and Hutson,S.M.
  TITLE     Branched chain aminotransferase isoenzymes. Purification and
            characterization of the rat brain isoenzyme
  JOURNAL   J Biol Chem 268 (5), 3092-3098 (1993)
   PUBMED   8381418
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Nov 26, 2020 this sequence version replaced NM_022400.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U68417.1, SRR26360186.534386.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                JAXUCZ010000001.1  105179139-105179169
            32-106              JAXUCZ010000001.1  105184103-105184177
            107-310             JAXUCZ010000001.1  105184636-105184839
            311-421             JAXUCZ010000001.1  105191663-105191773
            422-541             JAXUCZ010000001.1  105191856-105191975
            542-705             JAXUCZ010000001.1  105192116-105192279
            706-848             JAXUCZ010000001.1  105194551-105194693
            849-934             JAXUCZ010000001.1  105194851-105194936
            935-1075            JAXUCZ010000001.1  105195149-105195289
            1076-1150           JAXUCZ010000001.1  105195364-105195438
            1151-1544           JAXUCZ010000001.1  105196081-105196474
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..1544
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /note="branched chain amino acid transaminase 2"
                     /db_xref="GeneID:64203"
                     /db_xref="RGD:68948"
     exon            1..31
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     CDS             8..1189
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /EC_number="2.6.1.42"
                     /note="branched-chain-amino-acid aminotransferase,
                     mitochondrial; BCAT(m); branched chain aminotransferase 2,
                     mitochondrial; heart branched chain aminotransferase
                     precursor encoding mitochondrial protein; branched chain
                     amino acid transaminase 2, mitochondrial"
                     /codon_start=1
                     /product="branched-chain-amino-acid aminotransferase,
                     mitochondrial precursor"
                     /protein_id="NP_071795.2"
                     /db_xref="GeneID:64203"
                     /db_xref="RGD:68948"
                     /translation="MAAAILGQVWARKLLPIPWRLCVPGRCVSSNFKAADLQVQVTRE
                     PQKKPAPSQPLLFGKTFTDHMLMVEWNSKTGWGPPRIQPFQNLTLHPACSGLHYSLQL
                     FEGLKAYKGRDKQVRLFRPWLNMDRMLRSARRLCLPDFDKQELLECIRQLIEVDKDWV
                     PDGNGTSLYVRPVLIGNEPSLGVGMVTQALLFVILCPVGSYFPGDSMTPVSLLADPSF
                     VRAWIGGVGDCKLGGNYGPTVAVQQEAQKKGCEQVLWLYGPDHQLTEVGTMNIFVYWT
                     HEDGELELATPPLDGIILPGVVRQSLLDLARTWGEFRVAERKVTMKELKRALEEGRVR
                     EVFGSGTACQVCPVHQILYEGKQLHIPTMENGPELILRFQKELKAIQYGTSAHDWMLR
                     V"
     exon            32..106
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            107..310
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            311..421
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            422..541
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            542..705
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            706..848
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            849..934
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1075
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1150
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1544
                     /gene="Bcat2"
                     /gene_synonym="Bcatm; BCT2; mBcat"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgggatcatg gctgcagcta tactaggaca ggtctgggcc cggaagcttc tgcctattcc
       61 ctggcgtcta tgtgtgcccg gaagatgtgt gtcctccaac ttcaaggcgg cagacctcca
      121 ggttcaggtg accagagagc cacagaagaa gccagcccct agccagcctc tgctgtttgg
      181 gaagacattc acggaccaca tgctgatggt ggagtggaac agcaagacag gctggggccc
      241 tccaaggatc cagcccttcc agaacctcac actgcaccca gcctgctccg gcctgcacta
      301 ctcattgcag ctctttgaag gcttgaaagc ttataaaggc agagacaagc aggtacgcct
      361 cttccggccc tggctcaaca tggacaggat gctacgttct gcaaggcgcc tctgcctgcc
      421 agactttgac aagcaggaac tgctggagtg catccgccag ctcattgaag tagacaaaga
      481 ctgggttccc gatggcaatg gaacaagcct ctatgtgcgg ccggtgctta ttggaaatga
      541 gccctccctg ggtgttggaa tggtcacaca agccctcctg ttcgtcattc tgtgccctgt
      601 gggctcctac ttccccggag actccatgac acctgtctcc ctcctggcgg acccctcatt
      661 cgtcagagcc tggattggtg gggtcggtga ctgcaagttg ggggggaatt acggccccac
      721 tgtggctgtg caacaagaag cccagaagaa aggctgcgag caggtcctct ggctgtacgg
      781 gccggaccac cagctcaccg aggtgggaac catgaacatc tttgtctact ggactcatga
      841 agacggagaa ctggagctgg caaccccccc actggatggc atcatcctcc ccggagtggt
      901 tcgacaaagc ctgctggatc tggccaggac ttggggtgag ttccgggtgg cagaacgcaa
      961 ggtcactatg aaggaattga agcgggcact ggaggagggc cgggtgcgag aagtcttcgg
     1021 ctcaggcact gcttgtcagg tctgcccagt gcaccaaatc ctatatgaag gcaagcaact
     1081 ccacattcct accatggaaa atgggccgga actcatcctg cgcttccaga aggagttgaa
     1141 ggctattcag tacggaacca gtgcccacga ttggatgctt cgtgtgtgac gttagggagg
     1201 ttgtgcccta cctggttgga tccgctgacg tgcagcatcc agttcagcgt cctcgcctcc
     1261 cagagactca agagaagtgc aatagggaaa ggtcgttcca gactgctggc tctctggcac
     1321 cctcacgtgg aagttacact ccaaagcctt aagggcggga cccatgtaat ttggacccca
     1381 cctcagttca gctgctccgc gtcctggtgt tcaagagaga accttggctt cttctcgatc
     1441 tccgttctca ggccggatcc ccggtgctct tcatgtcttt tttttttttt tttccgtctc
     1501 tctctccttg ctgcaatttt gaaataaaat gccaaagaac aaga
//
