LOCUS       NM_017253               2465 bp    mRNA    linear   ROD 16-FEB-2024
DEFINITION  Rattus norvegicus branched chain amino acid transaminase 1 (Bcat1),
            transcript variant 1, mRNA.
ACCESSION   NM_017253
VERSION     NM_017253.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Garcia-Espinosa,M.A., Wallin,R., Hutson,S.M. and Sweatt,A.J.
  TITLE     Widespread neuronal expression of branched-chain aminotransferase
            in the CNS: implications for leucine/glutamate metabolism and for
            signaling by amino acids
  JOURNAL   J Neurochem 100 (6), 1458-1468 (2007)
   PUBMED   17348860
  REMARK    GeneRIF: this metabolic enzyme is expressed throughout the brain
            and spinal cord, with distinct differences in regional and
            intracellular patterns of expression.
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Bradamante,V., Krnic,Z., Zrinski,R., Konjevoda,P. and Reiner,Z.
  TITLE     Changes in butyrylcholinesterase activity and serum lipids after
            oxprenolol and glibenclamide treatments in non-diabetic rats
  JOURNAL   Arzneimittelforschung 56 (2), 64-69 (2006)
   PUBMED   16572919
REFERENCE   3  (bases 1 to 2465)
  AUTHORS   Goto,M., Miyahara,I., Hirotsu,K., Conway,M., Yennawar,N.,
            Islam,M.M. and Hutson,S.M.
  TITLE     Structural determinants for branched-chain aminotransferase
            isozyme-specific inhibition by the anticonvulsant drug gabapentin
  JOURNAL   J Biol Chem 280 (44), 37246-37256 (2005)
   PUBMED   16141215
REFERENCE   4  (bases 1 to 2465)
  AUTHORS   Wu,J.Y., Kao,H.J., Li,S.C., Stevens,R., Hillman,S., Millington,D.
            and Chen,Y.T.
  TITLE     ENU mutagenesis identifies mice with mitochondrial branched-chain
            aminotransferase deficiency resembling human maple syrup urine
            disease
  JOURNAL   J Clin Invest 113 (3), 434-440 (2004)
   PUBMED   14755340
REFERENCE   5  (bases 1 to 2465)
  AUTHORS   Kholodilov,N.G., Neystat,M., Oo,T.F., Hutson,S.M. and Burke,R.E.
  TITLE     Upregulation of cytosolic branched chain aminotransferase in
            substantia nigra following developmental striatal target injury
  JOURNAL   Brain Res Mol Brain Res 75 (2), 281-286 (2000)
   PUBMED   10686349
REFERENCE   6  (bases 1 to 2465)
  AUTHORS   Bixel,M.G., Hutson,S.M. and Hamprecht,B.
  TITLE     Cellular distribution of branched-chain amino acid aminotransferase
            isoenzymes among rat brain glial cells in culture
  JOURNAL   J Histochem Cytochem 45 (5), 685-694 (1997)
   PUBMED   9154155
REFERENCE   7  (bases 1 to 2465)
  AUTHORS   Hutson,S.M., Bledsoe,R.K., Hall,T.R. and Dawson,P.A.
  TITLE     Cloning and expression of the mammalian cytosolic branched chain
            aminotransferase isoenzyme
  JOURNAL   J Biol Chem 270 (51), 30344-30352 (1995)
   PUBMED   8530459
REFERENCE   8  (bases 1 to 2465)
  AUTHORS   Hall,T.R., Wallin,R., Reinhart,G.D. and Hutson,S.M.
  TITLE     Branched chain aminotransferase isoenzymes. Purification and
            characterization of the rat brain isoenzyme
  JOURNAL   J Biol Chem 268 (5), 3092-3098 (1993)
   PUBMED   8381418
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000151.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_017253.4.
            
            On Mar 25, 2021 this sequence version replaced NM_017253.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC087710.1, U35774.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760396
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-138               JACYVU010000151.1  14330315-14330452   c
            139-249             JACYVU010000151.1  14328981-14329091   c
            250-450             JACYVU010000151.1  14321285-14321485   c
            451-561             JACYVU010000151.1  14314431-14314541   c
            562-681             JACYVU010000151.1  14312618-14312737   c
            682-845             JACYVU010000151.1  14301667-14301830   c
            846-988             JACYVU010000151.1  14297641-14297783   c
            989-1074            JACYVU010000151.1  14291978-14292063   c
            1075-1215           JACYVU010000151.1  14289559-14289699   c
            1216-1290           JACYVU010000151.1  14287211-14287285   c
            1291-2465           JACYVU010000151.1  14281903-14283077   c
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q44"
     gene            1..2465
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /note="branched chain amino acid transaminase 1"
                     /db_xref="GeneID:29592"
                     /db_xref="RGD:2195"
     exon            1..138
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..1332
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /EC_number="2.6.1.42"
                     /note="isoform 1 is encoded by transcript variant 1;
                     branched-chain-amino-acid aminotransferase, cytosolic;
                     BCAT(c); branched chain aminotransferase 1, cytosolic;
                     branched chain amino acid transaminase 1, cytosolic"
                     /codon_start=1
                     /product="branched-chain-amino-acid aminotransferase,
                     cytosolic isoform 1"
                     /protein_id="NP_058949.1"
                     /db_xref="GeneID:29592"
                     /db_xref="RGD:2195"
                     /translation="MAYLSRATATLARQDCSNGCSASYAEEEELEASTESYDEEGGSE
                     ASTQTFRAKDLIITKADVLKKKPDPSSLVFGASFTDHMLMVEWTSKYGWDKPHIKPFE
                     NLSIHPAASVLHYAVELFEGLKAFRGVDNKIRLFRPDLNMKRMCRSAVRTTLPEFDKE
                     ELLQCVLQLIQLDREWVPYSTSASLYIRPTFIGIEPSLGVKKPSKALLFVILSPVGSY
                     FSNGTFSPVSLWANPKFVRSWKGGTGDFKMGCNYGSSLLAQCEAAENGCHQVLWLYGK
                     ENRITEVGTMNLFLYWINKDGEEELATPPLDGVILPGVTRQSILELGEEWGEFKVCER
                     HITMDDLSTALEENRVKEMFGSGTACVVCPVASILYKGQMLHIPTMENGHKLSSRIMA
                     KLTDIQYGRIKSEWTLELP"
     exon            139..249
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            250..450
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            451..561
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            562..681
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            682..845
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            846..988
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1074
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1215
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1290
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..2465
                     /gene="Bcat1"
                     /gene_synonym="Bcatc"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccatcatac ctctatcgcc tgccccgccg aactgcaacc ccgggctctc ggcagcgccg
       61 ctgtgccacg catttccacg cgcccgcagc tttgtcatgg cctacttgtc ccgggcaact
      121 gcaaccctgg cccgccagga ctgcagtaat ggatgttcgg catcgtatgc tgaagaagaa
      181 gaattagaag cttcgaccga gtcgtatgat gaagaaggag gatcagaagc ttcgacccag
      241 acgtttaggg ccaaagatct cattatcaca aaagccgatg tcttaaaaaa gaagcccgac
      301 cccagctcgc tggtctttgg agcttcgttc actgaccaca tgctgatggt ggagtggacc
      361 tccaagtatg gatgggataa gcctcacatc aagccttttg aaaacttgtc catacatccc
      421 gctgcctctg ttttgcacta tgctgtggaa ttgtttgaag gcttgaaggc ttttcgggga
      481 gttgataaca agatccgatt gttccggccg gacctcaaca tgaaaagaat gtgccgatct
      541 gccgtgagga ccactctgcc ggagtttgac aaggaggagc ttctgcaatg cgttcttcag
      601 ctcatacagc tcgaccgaga gtgggtcccc tactcaacct ctgccagtct gtacatccgc
      661 cccacattca tcgggattga gccatcgctt ggagtcaaga agccttctaa agctctgctc
      721 tttgtgattt tgagccccgt gggatcttat ttttctaatg ggaccttttc tccggtgtcc
      781 ctgtgggcca acccaaagtt cgtcagatcc tggaaaggtg ggactggaga cttcaagatg
      841 ggctgcaact atggatcttc ccttctggcg cagtgtgagg cggcagagaa cggctgtcac
      901 caggtcctgt ggctgtacgg caaggaaaac cggataactg aagtgggcac gatgaatctc
      961 ttcctctact ggataaacaa agatggagaa gaagaactgg cgacgcctcc gctagatggc
     1021 gtcattctcc caggagtcac tcggcagagc atcctggagc tgggagaaga gtggggcgag
     1081 tttaaggtgt gcgagagaca catcaccatg gatgacctgt ccaccgccct ggaggagaac
     1141 agagtgaagg agatgttcgg ctcggggaca gcctgcgttg tctgcccagt tgccagtatt
     1201 ctgtacaagg gccagatgct gcacattcca accatggaga atggccacaa gctttcaagt
     1261 cgcatcatgg caaagttgac tgatatccag tatggacgga tcaagagcga atggacactg
     1321 gagttgccct aatgggagag ctcagggtat cggtgacggg gtgccacaga agaccgactc
     1381 ctgtcctcga gctgggacaa cctgtgacta ttttaactcc ttgtagttcc attgttcatt
     1441 gttaccaaac gagtttgtgt cccccccccc ccccccgtga gccaagaaaa ttgccagtgt
     1501 taagtgtcac ctgtgaacat gatagtagct gctttactgt acctttcctt ggaaagctat
     1561 caatgaataa ttgcctcccc tcactccata ccagatcctt agtaggagaa tgggttcccc
     1621 caccaatgag atgactgtgt gctggaaggg ctactgagga aatgccatag gcggcctccc
     1681 tgtgactcat gggcaccctc agagcgggag ccctttgtta cttttgctgg ctggtaaggt
     1741 tctgttgttt acgtttttgt ttgtttgttt ctaattgggg aagatttggg ctccctgtga
     1801 agccctgctg gcctggtatt ccgtgatgtc tgcccaggct ggtctcagac tcggagcggg
     1861 tcttcctgtc tcacacagcc ttctggatgc tggggcgatg gacaggtgtg ataccttgat
     1921 ttgtttctgt cttttaaaca aaagtttggt cggctggatt tcgtttctcc gcgcttctgt
     1981 aagtccctga gtccgcgcta aggtgaccgt gccccttctt ggtatcatgg cctgcactaa
     2041 tgtcaccgaa ggtgtctgtg aggctcactg ccctttttct gtaactatac ctggctgctc
     2101 actgcgctgc tatgagacta ggggtcctgt gccttctaga tctttgtatc ctcaaagccc
     2161 agcacatagt aagtattcag tacttgttta ccatgagaat gaacatagaa gtgtactttc
     2221 tgtctctcca tgctgaaacc ctttcggaaa ccacgcctga ttcgggcagc atgtagcagt
     2281 atttatctta ggctgtcttt cttgtccctg gactcctcca gacagcatgg aggaattctg
     2341 ttttaagatt atcttggagt ttaagtgatt gccctggaaa aatgaaatgt accaaccatg
     2401 tggaaatgac agctactgta cattatgtat gaaatgccaa ataaagggac aagttgggag
     2461 ataaa
//
