LOCUS       NM_001002802            1784 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Rattus norvegicus beta-secretase 2 (Bace2), mRNA.
ACCESSION   NM_001002802 XM_213642 XM_340976
VERSION     NM_001002802.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Alcarraz-Vizan,G., Castano,C., Visa,M., Montane,J., Servitja,J.M.
            and Novials,A.
  TITLE     BACE2 suppression promotes beta-cell survival and function in a
            model of type 2 diabetes induced by human islet amyloid polypeptide
            overexpression
  JOURNAL   Cell Mol Life Sci 74 (15), 2827-2838 (2017)
   PUBMED   28337562
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Alcarraz-Vizan,G., Casini,P., Cadavez,L., Visa,M., Montane,J.,
            Servitja,J.M. and Novials,A.
  TITLE     Inhibition of BACE2 counteracts hIAPP-induced insulin secretory
            defects in pancreatic beta-cells
  JOURNAL   FASEB J 29 (1), 95-104 (2015)
   PUBMED   25342134
  REMARK    GeneRIF: found by global gene expression profiling that BACE2 was
            up-regulated in the rat pancreatic beta-cell line INS1E stably
            transfected with human IAPP gene
REFERENCE   3  (bases 1 to 1784)
  AUTHORS   Holler,C.J., Webb,R.L., Laux,A.L., Beckett,T.L., Niedowicz,D.M.,
            Ahmed,R.R., Liu,Y., Simmons,C.R., Dowling,A.L., Spinelli,A.,
            Khurgel,M., Estus,S., Head,E., Hersh,L.B. and Murphy,M.P.
  TITLE     BACE2 expression increases in human neurodegenerative disease
  JOURNAL   Am J Pathol 180 (1), 337-350 (2012)
   PUBMED   22074738
REFERENCE   4  (bases 1 to 1784)
  AUTHORS   Esterhazy,D., Stutzer,I., Wang,H., Rechsteiner,M.P., Beauchamp,J.,
            Dobeli,H., Hilpert,H., Matile,H., Prummer,M., Schmidt,A.,
            Lieske,N., Boehm,B., Marselli,L., Bosco,D., Kerr-Conte,J.,
            Aebersold,R., Spinas,G.A., Moch,H., Migliorini,C. and Stoffel,M.
  TITLE     Bace2 is a beta cell-enriched protease that regulates pancreatic
            beta cell function and mass
  JOURNAL   Cell Metab 14 (3), 365-377 (2011)
   PUBMED   21907142
REFERENCE   5  (bases 1 to 1784)
  AUTHORS   Bettegazzi,B., Mihailovich,M., Di Cesare,A., Consonni,A., Macco,R.,
            Pelizzoni,I., Codazzi,F., Grohovaz,F. and Zacchetti,D.
  TITLE     beta-Secretase activity in rat astrocytes: translational block of
            BACE1 and modulation of BACE2 expression
  JOURNAL   Eur J Neurosci 33 (2), 236-243 (2011)
   PUBMED   21073551
  REMARK    GeneRIF: Data show that astrocytes possess beta-secretase activity
            and produce amyloid-beta because of the activity of BACE2, but not
            BACE1, the expression of which is blocked at the translational
            level.
REFERENCE   6  (bases 1 to 1784)
  AUTHORS   Myllykangas,L., Wavrant-De Vrieze,F., Polvikoski,T., Notkola,I.L.,
            Sulkava,R., Niinisto,L., Edland,S.D., Arepalli,S., Adighibe,O.,
            Compton,D., Hardy,J., Haltia,M. and Tienari,P.J.
  TITLE     Chromosome 21 BACE2 haplotype associates with Alzheimer's disease:
            a two-stage study
  JOURNAL   J Neurol Sci 236 (1-2), 17-24 (2005)
   PUBMED   16023140
REFERENCE   7  (bases 1 to 1784)
  AUTHORS   Puente,X.S. and Lopez-Otin,C.
  TITLE     A genomic analysis of rat proteases and protease inhibitors
  JOURNAL   Genome Res 14 (4), 609-622 (2004)
   PUBMED   15060002
REFERENCE   8  (bases 1 to 1784)
  AUTHORS   Basi,G., Frigon,N., Barbour,R., Doan,T., Gordon,G., McConlogue,L.,
            Sinha,S. and Zeller,M.
  TITLE     Antagonistic effects of beta-site amyloid precursor
            protein-cleaving enzymes 1 and 2 on beta-amyloid peptide production
            in cells
  JOURNAL   J Biol Chem 278 (34), 31512-31520 (2003)
   PUBMED   12801932
REFERENCE   9  (bases 1 to 1784)
  AUTHORS   Yan,R., Munzner,J.B., Shuck,M.E. and Bienkowski,M.J.
  TITLE     BACE2 functions as an alternative alpha-secretase in cells
  JOURNAL   J Biol Chem 276 (36), 34019-34027 (2001)
   PUBMED   11423558
REFERENCE   10 (bases 1 to 1784)
  AUTHORS   Yan,R., Bienkowski,M.J., Shuck,M.E., Miao,H., Tory,M.C.,
            Pauley,A.M., Brashier,J.R., Stratman,N.C., Mathews,W.R., Buhl,A.E.,
            Carter,D.B., Tomasselli,A.G., Parodi,L.A., Heinrikson,R.L. and
            Gurney,M.E.
  TITLE     Membrane-anchored aspartyl protease with Alzheimer's disease
            beta-secretase activity
  JOURNAL   Nature 402 (6761), 533-537 (1999)
   PUBMED   10591213
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000011.1.
            
            On Oct 20, 2022 this sequence version replaced NM_001002802.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC166597.1, BN000318.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-326               JAXUCZ010000011.1  50176852-50177177
            327-415             JAXUCZ010000011.1  50224001-50224089
            416-632             JAXUCZ010000011.1  50230980-50231196
            633-761             JAXUCZ010000011.1  50234337-50234465
            762-896             JAXUCZ010000011.1  50235652-50235786
            897-998             JAXUCZ010000011.1  50236886-50236987
            999-1148            JAXUCZ010000011.1  50242849-50242998
            1149-1317           JAXUCZ010000011.1  50246142-50246310
            1318-1784           JAXUCZ010000011.1  50258482-50258948
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q12"
     gene            1..1784
                     /gene="Bace2"
                     /note="beta-secretase 2"
                     /db_xref="GeneID:288227"
                     /db_xref="RGD:1303241"
     exon            1..326
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1571
                     /gene="Bace2"
                     /EC_number="3.4.23.45"
                     /function="aspartyl protease"
                     /note="memapsin-1; theta-secretase; membrane-associated
                     aspartic protease 1; beta-site amyloid precursor protein
                     cleaving enzyme 2; beta-site APP-cleaving enzyme 2"
                     /codon_start=1
                     /product="beta-secretase 2 precursor"
                     /protein_id="NP_001002802.1"
                     /db_xref="GeneID:288227"
                     /db_xref="RGD:1303241"
                     /translation="MGALLRALLLPLLAQWLLRAVPVLAPAPFTLPLQVAGAANHRAS
                     TVPGLGTPELPRADGLALALEPARATANFLAMVDNLQGDSGRGYYLEMLIGTPPQKVR
                     ILVDTGSSNFAVAGAPHSYIDTYFDSESSSTYHSKGFEVTVKYTQGSWTGFVGEDLVT
                     IPKGFNSSFLVNIATIFESENFFLPGIKWNGILGLAYAALAKPSSSLETFFDSLVAQA
                     KIPDIFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILK
                     LEIGGQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARTSLIPEFSDGFWT
                     GAQLACWTNSETPWAYFPKISIYLRDENASRSFRITILPQLYIQPMMGAGFNYECYRF
                     GISSSTNALVIGATVMEGFYVVFDRAQRRVGFAVSPCAEIAGTTVSEISGPFSTEDIA
                     SNCVPAQALNEPILWIVSYALMSVCGAILLVLILLLLFPLHCRHAPRDPEVVNDESSL
                     VRHRWK"
     exon            327..415
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
     exon            416..632
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
     exon            633..761
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
     exon            762..896
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
     exon            897..998
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1148
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1317
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1784
                     /gene="Bace2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agactcgccg agcccccgcc gcggggatgg gcgcgctgct tcgagcactc ttgctcccac
       61 tgctggcgca gtggctcttg cgcgcggtcc ccgtgctggc cccggcgccc ttcacgcttc
      121 ccctccaagt ggccggggcc gcgaaccaca gagcctcaac tgttcccggg ctcgggaccc
      181 ccgagttgcc ccgggctgat ggcctggccc tcgcactgga gcctgccagg gctaccgcca
      241 acttcttggc tatggtggac aatcttcagg gggactctgg ccgcggctac tacctggaga
      301 tgctgattgg gacccctccg cagaaggtac ggattcttgt ggacaccgga agcagtaact
      361 tcgcagtggc aggtgcccca cactcctaca tagacaccta ctttgactca gagagctcca
      421 gcacatacca ctccaagggc tttgaggtca cggtgaagta cacacaggga agctggactg
      481 gatttgttgg tgaggacctt gtcaccatcc caaaaggctt caacagctct ttcttggtca
      541 atattgccac tattttcgag tctgagaatt tctttttgcc tgggattaag tggaatggaa
      601 tccttggact cgcttatgct gccttggcca agccatcaag ctctctggag actttttttg
      661 attccctggt ggcccaagca aaaattccgg acattttctc catgcagatg tgcggggctg
      721 gattgccagt agctggatct ggtaccaacg gaggtagtct tgtcctgggt gggattgaac
      781 caagtttgta taaaggagat atatggtata ccccaattaa agaggaatgg tactatcaaa
      841 tagagatctt gaaattggaa attggaggcc agagcctcaa cctggactgc agagagtata
      901 acgcagacaa ggccattgtg gacagcggca ccacactcct gcgcctgccc cagaaggtgt
      961 ttgatgcagt ggtggaagcc gtggcacgca catctctgat tccagagttt tctgatggct
     1021 tctggacggg ggcccagctg gcatgctgga caaattctga aactccatgg gcgtatttcc
     1081 ctaagatttc tatctacctg agagatgaga acgccagtcg ctccttccga atcaccattc
     1141 tgccgcagct ctacattcag cccatgatgg gagctggttt caattatgag tgctaccgtt
     1201 ttggtatctc ctcttccaca aatgcgctgg tgattggtgc caccgtgatg gagggattct
     1261 acgtggtctt tgacagagct cagaggaggg tgggctttgc agtgagtccc tgtgcagaga
     1321 ttgcaggtac cacagtgtct gaaatttctg ggcccttttc cacggaagac atagccagca
     1381 actgtgttcc agcacaggct ctgaatgagc ccatcttgtg gattgtgtcc tatgccctga
     1441 tgagtgtgtg tggagccatt ctcctggttc tgatcctcct gctgctgttc ccgctgcact
     1501 gccgacacgc cccccgagac cctgaggtgg ttaacgatga atcctcacta gtcagacatc
     1561 gctggaaatg aagagcctca tctgaactcc agcagccttg aactcagctc ttctaagagg
     1621 gatgtctcca actggcttct ctgtctgtta gtctggaact tcagccgggc atctgaaggc
     1681 cttccagact cgctgtatct tgattactct tgatttccaa gttttcaggt cttttctact
     1741 tcagagagaa atgataataa aaaacacctc attgtgaacc aaaa
//
