LOCUS       NM_019204               2158 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Rattus norvegicus beta-secretase 1 (Bace1), mRNA.
ACCESSION   NM_019204
VERSION     NM_019204.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Velazquez Toledano,J., Bello,M., Correa Basurto,J., Guerrero
            Gonzalez,I., Pacheco-Yepez,J. and Rosales Hernandez,M.C.
  TITLE     Determining Structural Changes for Ligand Recognition between Human
            and Rat Phosphorylated BACE1 in Silico and Its Phosphorylation by
            GSK3beta at Thr252 by in Vitro Studies
  JOURNAL   ACS Chem Neurosci 15 (3), 629-644 (2024)
   PUBMED   38227464
  REMARK    GeneRIF: Determining Structural Changes for Ligand Recognition
            between Human and Rat Phosphorylated BACE1 in Silico and Its
            Phosphorylation by GSK3beta at Thr252 by in Vitro Studies.
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Wang,X., Li,M. and Hu,Y.
  TITLE     miR-29c-3p Attenuates beta-Amyloid-Induced Neurotoxicity in
            Alzheimer's Disease Through Regulating beta-Site Amyloid Precursor
            Protein-Cleaving Enzyme 1
  JOURNAL   Physiol Res 72 (6), 833-841 (2023)
   PUBMED   38215068
  REMARK    GeneRIF: miR-29c-3p Attenuates beta-Amyloid-Induced Neurotoxicity
            in Alzheimer's Disease Through Regulating beta-Site Amyloid
            Precursor Protein-Cleaving Enzyme 1.
REFERENCE   3  (bases 1 to 2158)
  AUTHORS   Qu,J., Xiong,X., Hujie,G., Ren,J., Yan,L. and Ma,L.
  TITLE     MicroRNA-132-3p alleviates neuron apoptosis and impairments of
            learning and memory abilities in Alzheimer's disease by
            downregulation of HNRNPU stabilized BACE1
  JOURNAL   Cell Cycle 20 (21), 2309-2320 (2021)
   PUBMED   34585626
  REMARK    GeneRIF: MicroRNA-132-3p alleviates neuron apoptosis and
            impairments of learning and memory abilities in Alzheimer's disease
            by downregulation of HNRNPU stabilized BACE1.
REFERENCE   4  (bases 1 to 2158)
  AUTHORS   Babusikova,E., Dobrota,D., Turner,A.J. and Nalivaeva,N.N.
  TITLE     Effect of Global Brain Ischemia on Amyloid Precursor Protein
            Metabolism and Expression of Amyloid-Degrading Enzymes in Rat
            Cortex: Role in Pathogenesis of Alzheimer's Disease
  JOURNAL   Biochemistry (Mosc) 86 (6), 680-692 (2021)
   PUBMED   34225591
  REMARK    GeneRIF: Effect of Global Brain Ischemia on Amyloid Precursor
            Protein Metabolism and Expression of Amyloid-Degrading Enzymes in
            Rat Cortex: Role in Pathogenesis of Alzheimer's Disease.
REFERENCE   5  (bases 1 to 2158)
  AUTHORS   De Pietri Tonelli,D., Mihailovich,M., Di Cesare,A., Codazzi,F.,
            Grohovaz,F. and Zacchetti,D.
  TITLE     Translational regulation of BACE-1 expression in neuronal and
            non-neuronal cells
  JOURNAL   Nucleic Acids Res 32 (5), 1808-1817 (2004)
   PUBMED   15034149
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2158)
  AUTHORS   Rogers,G.W. Jr., Edelman,G.M. and Mauro,V.P.
  TITLE     Differential utilization of upstream AUGs in the beta-secretase
            mRNA suggests that a shunting mechanism regulates translation
  JOURNAL   Proc Natl Acad Sci U S A 101 (9), 2794-2799 (2004)
   PUBMED   14981268
REFERENCE   7  (bases 1 to 2158)
  AUTHORS   Yan,R., Han,P., Miao,H., Greengard,P. and Xu,H.
  TITLE     The transmembrane domain of the Alzheimer's beta-secretase (BACE1)
            determines its late Golgi localization and access to beta -amyloid
            precursor protein (APP) substrate
  JOURNAL   J Biol Chem 276 (39), 36788-36796 (2001)
   PUBMED   11466313
REFERENCE   8  (bases 1 to 2158)
  AUTHORS   Lin,X., Koelsch,G., Wu,S., Downs,D., Dashti,A. and Tang,J.
  TITLE     Human aspartic protease memapsin 2 cleaves the beta-secretase site
            of beta-amyloid precursor protein
  JOURNAL   Proc Natl Acad Sci U S A 97 (4), 1456-1460 (2000)
   PUBMED   10677483
REFERENCE   9  (bases 1 to 2158)
  AUTHORS   Vassar,R., Bennett,B.D., Babu-Khan,S., Kahn,S., Mendiaz,E.A.,
            Denis,P., Teplow,D.B., Ross,S., Amarante,P., Loeloff,R., Luo,Y.,
            Fisher,S., Fuller,J., Edenson,S., Lile,J., Jarosinski,M.A.,
            Biere,A.L., Curran,E., Burgess,T., Louis,J.C., Collins,F.,
            Treanor,J., Rogers,G. and Citron,M.
  TITLE     Beta-secretase cleavage of Alzheimer's amyloid precursor protein by
            the transmembrane aspartic protease BACE
  JOURNAL   Science 286 (5440), 735-741 (1999)
   PUBMED   10531052
REFERENCE   10 (bases 1 to 2158)
  AUTHORS   Urmoneit,B., Reinsch,C., Turner,J., Czech,C., Beyreuther,K. and
            Dyrks,T.
  TITLE     Inhibition of beta A4 production by specific modulation of
            beta-secretase activity
  JOURNAL   J Mol Neurosci 6 (1), 23-32 (1995)
   PUBMED   8562317
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF190727.1 and JAXUCZ010000008.1.
            
            On Jan 31, 2014 this sequence version replaced NM_019204.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF190727.1, SRR26643287.19966.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            regulatory uORF        :: PMID: 14981268
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1785              AF190727.1         1-1785
            1786-2158           JAXUCZ010000008.1  55060766-55061138
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     gene            1..2158
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /note="beta-secretase 1"
                     /db_xref="GeneID:29392"
                     /db_xref="RGD:2191"
     exon            1..688
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
     regulatory      40..51
                     /regulatory_class="uORF"
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:14981268]"
                     /note="this uORF is predicted to encode a 3 aa peptide
                     (PMID:14981268)"
     regulatory      138..209
                     /regulatory_class="uORF"
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:14981268]"
                     /note="this uORF is predicted to encode a 23 aa peptide
                     (PMID:14981268)"
     regulatory      204..209
                     /regulatory_class="uORF"
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:14981268]"
                     /note="this uORF is predicted to encode 1 aa
                     (PMID:14981268)"
     regulatory      326..349
                     /regulatory_class="uORF"
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:14981268]"
                     /note="this uORF is predicted to encode a 7 aa peptide
                     (PMID:14981268)"
     CDS             428..1933
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /EC_number="3.4.23.46"
                     /note="ASP2; asp 2; memapsin-2; aspartyl protease 2;
                     membrane-associated aspartic protease 2; beta-site amyloid
                     precursor protein cleaving enzyme 1; beta-site APP
                     cleaving enzyme 1"
                     /codon_start=1
                     /product="beta-secretase 1 precursor"
                     /protein_id="NP_062077.1"
                     /db_xref="GeneID:29392"
                     /db_xref="RGD:2191"
                     /translation="MAPALRWLLLWVGSGMLPAQGTHLGIRLPLRSGLAGPPLGLRLP
                     RETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVG
                     AAPHPFLHRYYQRQLSSTYRDLRKSVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANI
                     AAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGA
                     GFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKM
                     DCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAG
                     TTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGT
                     VMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQT
                     DESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK"
     exon            689..777
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
     exon            778..994
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1132
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1267
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1369
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1519
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1691
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..2158
                     /gene="Bace1"
                     /gene_synonym="Bace"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccccagcctg cctaggtgct gggagccggg agctggatta tggtggcctg agcagccgac
       61 gcagccgcag gagctgggag tccctcacgc tgcaaagtcc gcctggaaga ccctgaaagc
      121 tgcaggctcc gatagccatg cccgcccctc ccagccccac aaggggcccg atccccccgc
      181 tgaggctggc ggtcgccgtc cagatgtagc tgggtccccc ggatcgccat cgtcctcttc
      241 tctcgtgcgc tacagatttc tcctgcccac tctccaccgc cgggagcagg aactgagcga
      301 ggggcctgca gactctgcag tcctgatgcc cccgaggccg ctctcctgag agaagccacc
      361 accacccaga cttaggggca ggcaagaggg acagtcgcca accggagcca caaggcccgg
      421 gctcaccatg gccccggcgc tgcgctggct cctgctatgg gtgggctcgg gaatgctgcc
      481 tgcccaggga acccatctcg gtatccgact gccccttcgc agcggcctgg cagggccacc
      541 cctgggcctg aggctgcccc gggagacgga cgaggaacct gaggagcctg gccggagagg
      601 cagctttgtg gagatggtgg acaacctgag gggaaagtcc ggccagggct actatgtgga
      661 gatgaccgtg ggcagccccc cacagacgct caacatcctg gtggacacgg gcagtagtaa
      721 ttttgcagtg ggggctgccc cacacccttt cctgcatcga tactaccaaa ggcagctgtc
      781 cagtacatac cgagacctcc gaaagtctgt gtatgtgccc tacacccagg gcaagtggga
      841 gggggaactg ggcactgacc tggtgagcat ccctcatggc cccaacgtca ctgtgcgtgc
      901 caacattgct gccatcactg aatcggacaa gttcttcatc aatggttcca actgggaggg
      961 catcctaggg ctggcctatg ctgagattgc caggcctgac gactccttgg agcccttttt
     1021 tgactccctg gtgaagcaga cccacattcc gaacatcttt tccctgcagc tctgtggcgc
     1081 tggcttcccc ctcaaccaga ctgaggcact ggcctcggtg ggagggagca tgatcattgg
     1141 tggtatcgac cattccctat acactggcag tctctggtac acacccatcc ggcgggagtg
     1201 gtattatgaa gtgatcattg tacgtgtaga aatcaatggt caagatctga aaatggactg
     1261 caaggagtac aactatgaca agagcatcgt ggacagtggc accaccaacc ttcgtttgcc
     1321 caagaaagta tttgaagctg cagtcaagtc catcaaggca gcctcctcga cggagaagtt
     1381 cccggatggc ttttggctag gggagcagct ggtgtgctgg caagcaggca cgaccccttg
     1441 gaacattttc ccagtcattt cactttacct catgggtgaa gtcaccaatc agtccttccg
     1501 catcaccatc cttcctcagc aatacctacg gccagtggaa gatgtggcca cgtcccaaga
     1561 cgactgttac aagttcgccg tctcacagtc atccacaggc accgttatgg gagcggtcat
     1621 catggaaggc ttctatgtgg tctttgatcg agcccgaaag cgaattggct ttgctgtcag
     1681 cgcttgccat gtgcacgatg agttcaggac ggcggcagtg gaaggtccgt ttgtcacggc
     1741 agacatggaa gactgtggct acaacattcc acagacagat gagtcgacac ttatgaccat
     1801 agcctatgtc atggctgcca tctgcgccct cttcatgttg ccactctgcc tcatggtatg
     1861 tcagtggcgc tgcctacgct gcctgcgcca tcagcatgat gactttgctg atgacatctc
     1921 cctgctgaaa taaggaggcc agtgggcaga tgacagagat ccccctggac cacatctggg
     1981 tggttccctt tggtcacgtg agttggagat atggatggta cctgtggcca gagcacctca
     2041 ggaccctcac caacctgccg aatgcttctg ccttgacaga aaagagacag ctggcaagct
     2101 ggattacagg gcttgcaagg cctgtaggaa acaggaggga gaaagcagca ttctggtg
//
