LOCUS       NM_022860               2270 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Rattus norvegicus beta-1,4-N-acetyl-galactosaminyl transferase 1
            (B4galnt1), mRNA.
ACCESSION   NM_022860
VERSION     NM_022860.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Rabionet,M., van der Spoel,A.C., Chuang,C.C., von
            Tumpling-Radosta,B., Litjens,M., Bouwmeester,D., Hellbusch,C.C.,
            Korner,C., Wiegandt,H., Gorgas,K., Platt,F.M., Grone,H.J. and
            Sandhoff,R.
  TITLE     Male germ cells require polyenoic sphingolipids with complex
            glycosylation for completion of meiosis: a link to ceramide
            synthase-3
  JOURNAL   J Biol Chem 283 (19), 13357-13369 (2008)
   PUBMED   18308723
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Liu,Y., Wada,R., Kawai,H., Sango,K., Deng,C., Tai,T.,
            McDonald,M.P., Araujo,K., Crawley,J.N., Bierfreund,U., Sandhoff,K.,
            Suzuki,K. and Proia,R.L.
  TITLE     A genetic model of substrate deprivation therapy for a
            glycosphingolipid storage disorder
  JOURNAL   J Clin Invest 103 (4), 497-505 (1999)
   PUBMED   10021458
REFERENCE   3  (bases 1 to 2270)
  AUTHORS   Takamiya,K., Yamamoto,A., Furukawa,K., Yamashiro,S., Shin,M.,
            Okada,M., Fukumoto,S., Haraguchi,M., Takeda,N., Fujimura,K.,
            Sakae,M., Kishikawa,M., Shiku,H., Furukawa,K. and Aizawa,S.
  TITLE     Mice with disrupted GM2/GD2 synthase gene lack complex gangliosides
            but exhibit only subtle defects in their nervous system
  JOURNAL   Proc Natl Acad Sci U S A 93 (20), 10662-10667 (1996)
   PUBMED   8855236
REFERENCE   4  (bases 1 to 2270)
  AUTHORS   Ruan,S., Raj,B.K., Furukawa,K. and Lloyd,K.O.
  TITLE     Analysis of melanoma cells stably transfected with beta 1,4GalNAc
            transferase (GM2/GD2 synthase) cDNA: relative glycosyltransferase
            levels play a dominant role in determining ganglioside expression
  JOURNAL   Arch Biochem Biophys 323 (1), 11-18 (1995)
   PUBMED   7487055
REFERENCE   5  (bases 1 to 2270)
  AUTHORS   Yamashiro,S., Haraguchi,M., Furukawa,K., Takamiya,K., Yamamoto,A.,
            Nagata,Y., Lloyd,K.O., Shiku,H. and Furukawa,K.
  TITLE     Substrate specificity of beta 1,4-N-acetylgalactosaminyltransferase
            in vitro and in cDNA-transfected cells. GM2/GD2 synthase
            efficiently generates asialo-GM2 in certain cells
  JOURNAL   J Biol Chem 270 (11), 6149-6155 (1995)
   PUBMED   7890749
REFERENCE   6  (bases 1 to 2270)
  AUTHORS   Hidari,J.K., Ichikawa,S., Furukawa,K., Yamasaki,M. and
            Hirabayashi,Y.
  TITLE     beta 1-4N-acetylgalactosaminyltransferase can synthesize both
            asialoglycosphingolipid GM2 and glycosphingolipid GM2 in vitro and
            in vivo: isolation and characterization of a beta
            1-4N-acetylgalactosaminyltransferase cDNA clone from rat ascites
            hepatoma cell line AH7974F
  JOURNAL   Biochem J 303 (Pt 3) (Pt 3), 957-965 (1994)
   PUBMED   7980468
REFERENCE   7  (bases 1 to 2270)
  AUTHORS   Nagata,Y., Yamashiro,S., Yodoi,J., Lloyd,K.O., Shiku,H. and
            Furukawa,K.
  TITLE     Expression cloning of beta 1,4 N-acetylgalactosaminyltransferase
            cDNAs that determine the expression of GM2 and GD2 gangliosides
  JOURNAL   J Biol Chem 267 (17), 12082-12089 (1992)
   PUBMED   1601877
  REMARK    Erratum:[J Biol Chem. 1994 Mar 4;269(9):7045.. PMID: 8120069]
REFERENCE   8  (bases 1 to 2270)
  AUTHORS   Iber,H., Zacharias,C. and Sandhoff,K.
  TITLE     The c-series gangliosides GT3, GT2 and GP1c are formed in rat liver
            Golgi by the same set of glycosyltransferases that catalyse the
            biosynthesis of asialo-, a- and b-series gangliosides
  JOURNAL   Glycobiology 2 (2), 137-142 (1992)
   PUBMED   1606358
REFERENCE   9  (bases 1 to 2270)
  AUTHORS   Pohlentz,G., Klein,D., Schwarzmann,G., Schmitz,D. and Sandhoff,K.
  TITLE     Both GA2, GM2, and GD2 synthases and GM1b, GD1a, and GT1b synthases
            are single enzymes in Golgi vesicles from rat liver
  JOURNAL   Proc Natl Acad Sci U S A 85 (19), 7044-7048 (1988)
   PUBMED   3140234
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081799.1.
            
            On Sep 16, 2004 this sequence version replaced NM_022860.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081799.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q22"
     gene            1..2270
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /note="beta-1,4-N-acetyl-galactosaminyl transferase 1"
                     /db_xref="GeneID:64828"
                     /db_xref="RGD:620490"
     exon            1..140
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            141..359
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..1743
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /EC_number="2.4.1.92"
                     /note="GM2/GD2 synthase;
                     (N-acetylneuraminyl)-galactosylglucosylceramide;
                     beta-4N-acetylgalactosaminyltransferase;
                     UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-
                     galactosylglucosylceramide-beta-1,
                     4-N-acetylgalactosaminyltransferase; GA2 synthase; GM2
                     synthase"
                     /codon_start=1
                     /product="beta-1,4 N-acetylgalactosaminyltransferase 1"
                     /protein_id="NP_074051.1"
                     /db_xref="GeneID:64828"
                     /db_xref="RGD:620490"
                     /translation="MRLDRRALYALVLLLACASLGLLYASTRDAPGLPNPLALWSPPQ
                     GPPRLDLLDLATEPRYAHIPVRIKEQVVGLLAQNNCSCESSGGRFALPFLRQVRAIDF
                     TKAFDAEELRAVSISREQEYQAFLARSRSLADQLLIAPANSPLQYPLQGVEVQPLRSI
                     LVPGLSLQEASVQEIYQVNLIASLGTWDVAGEVTGVTLTGEGQSDLTLASPILDKLNR
                     QLQLVTYSSRSYQANTADTVRFSTKGHEVAFTILIRHPPNPRLYPPSSLPQGAQYNIS
                     ALVTVATKTFLRYDRLRALIASIRRFYPTVTIVIADDSDKPERISDPHVEHYFMPFGK
                     GWFAGRNLAVSQVTTKYVLWVDDDFVFTARTRLEKLVDVLERTPLDLVGGAVREISGY
                     ATTYRQLLSVEPGAPGFGNCLRQKQGFHHELAGFPNCVVTDGVVNFFLARTDKVRQVG
                     FDPRLNRVAHLEFFLDGLGSLRVGSCSDVVVDHASKVKLPWTSKDPGAELYARYRYPG
                     SLDQSQVAKHRLLFFKHRLQCMTAE"
     exon            360..524
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            525..631
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            632..672
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            673..853
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            854..952
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1143
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1284
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1525
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..2238
                     /gene="B4galnt1"
                     /gene_synonym="Galgt1; GalNAc-T"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcggatccc cgacactgtc gggctcccgc ccagctcccg catcaggccg tcgccggcct
       61 catgaggaac ccccggctcg ggcggagaac cgggcagtgc ccggaccgcg atcctgccgc
      121 ggtcttagaa cgttagacag gatgcggctg gaccgccggg ccctctatgc gctagttctg
      181 ctgcttgcct gcgcctcgct gggtctcctg tacgccagca cccgagacgc gccaggtctc
      241 ccgaaccctc tggcattgtg gtcaccccca caaggtcccc cgaggctcga tctgctagac
      301 cttgccactg agcctcgcta cgcacacatc ccagtcagga tcaaggagca agtggtgggg
      361 ctgctggctc agaacaattg cagttgtgag tccagcggag gacgctttgc cttgccgttc
      421 ctgaggcagg tccgggcgat tgacttcact aaagcctttg acgccgagga gctgagggct
      481 gtttctatct ccagagagca ggaataccag gccttccttg caaggagccg gtccctggct
      541 gaccagctgc tgatagcccc tgccaactcc cccttacagt atcccctgca gggtgtggag
      601 gttcagcccc tcaggagcat cctggtgcca gggctaagtc tgcaggaagc ttctgttcag
      661 gaaatatatc aggtgaacct gattgcttcc cttggcacct gggatgtggc aggggaagta
      721 acaggggtga ctctcactgg agaggggcag tcggacctca cccttgccag cccaattctg
      781 gataaactca accgacagct gcaactggtg acttacagca gccggagcta ccaagccaac
      841 acagcagaca cagtccggtt ctccaccaag ggacatgaag tggccttcac catcctcata
      901 agacatcctc ccaacccccg gctgtaccca ccatcatccc taccccaagg agcccagtac
      961 aacatcagtg ctctggttac cgttgccacc aagacctttc ttcgttatga tcggctacgg
     1021 gcactcattg ccagcatcag acgcttttac cctacggtca ccatagtaat cgctgacgac
     1081 agcgacaaac cggagcgaat tagcgacccc catgtggagc actatttcat gcccttcggc
     1141 aagggttggt ttgcaggtcg gaacctggcg gtgtcccaag taaccaccaa atacgtgctg
     1201 tgggtggacg acgactttgt cttcacggcg cgcacgcggc tggagaagct tgtggatgtc
     1261 ctggagagga cgcccctgga cttggttggg ggcgcggtgc gggagatctc gggctacgct
     1321 accacctacc gacagctgct aagtgtggag ccgggcgccc caggctttgg gaactgcctc
     1381 cggcaaaagc agggcttcca ccacgagctc gctggctttc caaactgcgt ggtcaccgac
     1441 ggcgtagtca acttcttcct ggcgcgcaca gataaagtgc gccaggtggg ctttgaccca
     1501 cgcctcaacc gggtggctca tctggaattc ttcctggatg gtcttggttc ccttcgagtt
     1561 ggctcctgct ctgatgttgt tgtggatcat gcgtcaaagg tgaagctgcc ttggacatca
     1621 aaggatccag gggctgaact ttatgcccgt taccgttacc cgggatcact ggaccaaagt
     1681 caggtggcca aacatcgact gctcttcttc aaacaccggc tacagtgcat gaccgccgag
     1741 taacgtctga tttgggcctt cacactgtca ggctgggcct gcctcctccc tgccaggaat
     1801 ttccagcaac cacccccccc caatccctga gcaccccact gatgaacacc ctggcttccc
     1861 gaccctctcc accaatctga ttcctaacag gggcttgtcc tggtgacacc cttcctttct
     1921 gtgagtgacc agaggccaga tggagccata tcctccccca cagccagtgc caagtcctcc
     1981 ccaaccccac tcctatgggg caggaaatgg ggaggttcac tttccaagtg ccaaagagcc
     2041 cagacggact ctaagaccct caagtggaaa cactctcacc tcctgaggtg ggcagggaaa
     2101 ctcccaattt gcaaccccag ggacatgcac cccaccccag ctctggatcc agcaccatgt
     2161 gtcccggctc caacataccc ctacagaaag cactgtgact gtagttctgt ggggctggtg
     2221 aacacacggt ggaagccaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
