LOCUS       NM_001191724            1628 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus acyl-CoA wax alcohol acyltransferase 2 (Awat2),
            mRNA.
ACCESSION   NM_001191724 XM_228583
VERSION     NM_001191724.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,Z., Onorato,J.M., Chen,L., Nelson,D.W., Yen,C.E. and Cheng,D.
  TITLE     Synthesis of neutral ether lipid monoalkyl-diacylglycerol by lipid
            acyltransferases
  JOURNAL   J Lipid Res 58 (6), 1091-1099 (2017)
   PUBMED   28420705
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Arne,J.M., Widjaja-Adhi,M.A.K., Hughes,T., Huynh,K.W.,
            Silvaroli,J.A., Chelstowska,S., Moiseenkova-Bell,V.Y. and
            Golczak,M.
  TITLE     Allosteric modulation of the substrate specificity of acyl-CoA wax
            alcohol acyltransferase 2
  JOURNAL   J Lipid Res 58 (4), 719-730 (2017)
   PUBMED   28096191
REFERENCE   3  (bases 1 to 1628)
  AUTHORS   Kaylor,J.J., Cook,J.D., Makshanoff,J., Bischoff,N., Yong,J. and
            Travis,G.H.
  TITLE     Identification of the 11-cis-specific retinyl-ester synthase in
            retinal Muller cells as multifunctional O-acyltransferase (MFAT)
  JOURNAL   Proc Natl Acad Sci U S A 111 (20), 7302-7307 (2014)
   PUBMED   24799687
REFERENCE   4  (bases 1 to 1628)
  AUTHORS   Yen,C.L., Brown,C.H. 4th, Monetti,M. and Farese,R.V. Jr.
  TITLE     A human skin multifunctional O-acyltransferase that catalyzes the
            synthesis of acylglycerols, waxes, and retinyl esters
  JOURNAL   J Lipid Res 46 (11), 2388-2397 (2005)
   PUBMED   16106050
REFERENCE   5  (bases 1 to 1628)
  AUTHORS   Turkish,A.R., Henneberry,A.L., Cromley,D., Padamsee,M., Oelkers,P.,
            Bazzi,H., Christiano,A.M., Billheimer,J.T. and Sturley,S.L.
  TITLE     Identification of two novel human acyl-CoA wax alcohol
            acyltransferases: members of the diacylglycerol acyltransferase 2
            (DGAT2) gene superfamily
  JOURNAL   J Biol Chem 280 (15), 14755-14764 (2005)
   PUBMED   15671038
REFERENCE   6  (bases 1 to 1628)
  AUTHORS   Cheng,J.B. and Russell,D.W.
  TITLE     Mammalian wax biosynthesis. II. Expression cloning of wax synthase
            cDNAs encoding a member of the acyltransferase enzyme family
  JOURNAL   J Biol Chem 279 (36), 37798-37807 (2004)
   PUBMED   15220349
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On Sep 1, 2022 this sequence version replaced NM_001191724.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643295.61052.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               JAXUCZ010000021.1  69530518-69530664   c
            148-258             JAXUCZ010000021.1  69525014-69525124   c
            259-329             JAXUCZ010000021.1  69524595-69524665   c
            330-534             JAXUCZ010000021.1  69524165-69524369   c
            535-709             JAXUCZ010000021.1  69523781-69523955   c
            710-909             JAXUCZ010000021.1  69522702-69522901   c
            910-1628            JAXUCZ010000021.1  69521778-69522496   c
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq22"
     gene            1..1628
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /note="acyl-CoA wax alcohol acyltransferase 2"
                     /db_xref="GeneID:302425"
                     /db_xref="RGD:1565111"
     exon            1..147
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..1064
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /EC_number="2.3.1.75"
                     /note="diacylglycerol O-acyltransferase 2-like 4"
                     /codon_start=1
                     /product="acyl-CoA wax alcohol acyltransferase 2"
                     /protein_id="NP_001178653.1"
                     /db_xref="GeneID:302425"
                     /db_xref="RGD:1565111"
                     /translation="MFWPTKKDLKTAMEVFALFQWALSALVIVITVIMVNLYLVVFTS
                     FWPVTVLMLLWLAFDWKTPERGGRRFNCVRRWCLWKHYSNYFPLKIVKTKDIPTNHNY
                     ILACHPHGLLSHSWFGHFSTDTTGFSKIFPGITPYMLTLGAFFWVPFLREYIMSTGAC
                     SVSQSSIDFLLTQKGTGNMLVVVVGGLAECKYSMPGSTTLFLKKRQGFVRTALRYGVS
                     LIPAYAFGEADLYDQHIFTPGSFISHFQKWFQKMVHVYPCAFYGRGFTKNSWGLLPYS
                     QPVTTIVGEPLPLPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELVII"
     exon            148..258
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /inference="alignment:Splign:2.1.0"
     exon            259..329
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /inference="alignment:Splign:2.1.0"
     exon            330..534
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /inference="alignment:Splign:2.1.0"
     exon            535..709
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /inference="alignment:Splign:2.1.0"
     exon            710..909
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1628
                     /gene="Awat2"
                     /gene_synonym="Dgat2l4; RGD1565111"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagtagtca gggctggaca gccaaacagc atttacctgg aactactgag attttgcaca
       61 tcatgttctg gcctaccaag aaggacctca agactgccat ggaggtcttt gctcttttcc
      121 agtgggccct cagcgccttg gttatagtca tcactgtgat catggtcaac ctctacttgg
      181 tggtgttcac atcgttctgg cctgtcactg tgttgatgct cctctggttg gcttttgact
      241 ggaagacacc tgagcgaggt ggccgtcggt tcaactgtgt gagaaggtgg tgtctgtgga
      301 aacactacag caattacttc ccactcaaga ttgtaaagac aaaagatatt ccaaccaacc
      361 acaactacat ccttgcctgc catcctcatg ggctgctgtc ccattcatgg tttggtcact
      421 tttccactga cacaacaggc ttttccaaga tctttcctgg tatcactcct tatatgctca
      481 cattaggagc ctttttctgg gtgcctttcc tcagagagta tataatgtct acaggggcct
      541 gctctgtgag tcaatcgtcc atagactttt tgcttactca gaagggcaca ggcaacatgc
      601 ttgttgtggt ggttggtggc ctggctgagt gcaaatacag catgcccggc tctaccaccc
      661 tgttcttgaa gaaacggcag ggctttgtac gcacagccct tcgatatggg gtgtctctaa
      721 tccccgctta tgcctttgga gaggcagacc tctatgacca gcacattttc actcctggga
      781 gctttatcag tcactttcag aagtggttcc agaagatggt acacgtctac ccctgtgctt
      841 tctatggacg tggcttcacc aagaactcct ggggccttct gccatattct cagccagtaa
      901 ccactattgt cggggaacct ctaccattgc ccaagattga gaatccgagc caggagattg
      961 tggccaaata ccacacactc tatattgatg ccctacgcaa attgttcgat cagcataaga
     1021 ccaagtttgg catctcagag acccaggagc tggtgataat ttgaaaagca ttccactgac
     1081 tggatggtgt ggaactgatg acaacggcca aagttaccca tggagcgtca cttctgattt
     1141 taccaatcct cactggaggg atgtgtgtgt ggcagggggg tgcatccatg tggttgtttt
     1201 ctgtgtccat ccttttccac ctccatctct ttctcttagt ctttgtcctt tcctcctgag
     1261 acagagaaga ccctaagcag aggtctgcta aagtcagtgg aagttcataa aagctttagc
     1321 cacaaggaaa cctgtggcta gaataaacag cccatttctg gtactgtgtg ctccagctag
     1381 tctgtttgga gggatctaag acattgtgtc tctgtcattt tcctggcttc aagactctta
     1441 taggactggg tatatcaaga gccaggggac attgggttca gtcagtagca tgttcaagac
     1501 ctatgagttt taggggtggt ccatcatcct gtttcttcaa ctgtaaaatg ggactaacaa
     1561 tccttccctt gcttacctca aagtttctgt caagtttaaa tgctactgca tgtgactaaa
     1621 catgataa
//
