LOCUS       NM_133288               5090 bp    mRNA    linear   ROD 16-FEB-2024
DEFINITION  Rattus norvegicus ATPase plasma membrane Ca2+ transporting 3
            (Atp2b3), mRNA.
ACCESSION   NM_133288 XM_001057885 XM_343839
VERSION     NM_133288.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Varshney,S., Adela,R., Kachhawa,G., Dada,R., Kulshreshtha,V.,
            Kumari,R., Agarwal,R. and Khadgawat,R.
  TITLE     Disrupted placental vitamin D metabolism and calcium signaling in
            gestational diabetes and pre-eclampsia patients
  JOURNAL   Endocrine 80 (1), 191-200 (2023)
   PUBMED   36477942
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Radzik,T., Boczek,T., Ferenc,B., Studzian,M., Pulaski,L. and
            Zylinska,L.
  TITLE     Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12
            Cells
  JOURNAL   Biomed Res Int 2019, 9616248 (2019)
   PUBMED   31032369
  REMARK    GeneRIF: Although modification of PMCAs composition could provide
            some protection against calcium overload, reduction of PMCA2
            appeared to be more detrimental to the cells than deficiency of
            PMCA3. Under pathological conditions, including inflammatory CCL5
            action and long-lasting Ca(2+) dyshomeostasis, insufficient cell
            protection may result in progressive degeneration and death of
            neurons.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5090)
  AUTHORS   Boczek,T., Lisek,M., Ferenc,B. and Zylinska,L.
  TITLE     Cross talk among PMCA, calcineurin and NFAT transcription factors
            in control of calmodulin gene expression in differentiating PC12
            cells
  JOURNAL   Biochim Biophys Acta Gene Regul Mech 1860 (4), 502-515 (2017)
   PUBMED   28153703
  REMARK    GeneRIF: higher occupancy of Calm2 and Calm3 promoters by NFATc2
            and increased expression of these genes in response to NFATc2
            silencing were demonstrated in PMCA2 and PMCA3-reduced lines.
REFERENCE   4  (bases 1 to 5090)
  AUTHORS   Figueroa,K.P., Paul,S., Cali,T., Lopreiato,R., Karan,S.,
            Frizzarin,M., Ames,D., Zanni,G., Brini,M., Dansithong,W.,
            Milash,B., Scoles,D.R., Carafoli,E. and Pulst,S.M.
  TITLE     Spontaneous shaker rat mutant - a new model for X-linked
            tremor/ataxia
  JOURNAL   Dis Model Mech 9 (5), 553-562 (2016)
   PUBMED   27013529
REFERENCE   5  (bases 1 to 5090)
  AUTHORS   de Juan-Sanz,J., Nunez,E., Zafra,F., Berrocal,M., Corbacho,I.,
            Ibanez,I., Arribas-Gonzalez,E., Marcos,D., Lopez-Corcuera,B.,
            Mata,A.M. and Aragon,C.
  TITLE     Presynaptic control of glycine transporter 2 (GlyT2) by physical
            and functional association with plasma membrane Ca2+-ATPase (PMCA)
            and Na+-Ca2+ exchanger (NCX)
  JOURNAL   J Biol Chem 289 (49), 34308-34324 (2014)
   PUBMED   25315779
  REMARK    GeneRIF: GlyT2 activity is pre-synaptically controlled by physical
            and functional association with plasma membrane PMCA2, PMCA3, and
            NCX1.
REFERENCE   6  (bases 1 to 5090)
  AUTHORS   Burk,S.E., Menon,A.G. and Shull,G.E.
  TITLE     Analysis of the 5' end of the rat plasma membrane Ca(2+)-ATPase
            isoform 3 gene and identification of extensive trinucleotide repeat
            sequences in the 5' untranslated region
  JOURNAL   Biochim Biophys Acta 1240 (2), 119-124 (1995)
   PUBMED   8541282
REFERENCE   7  (bases 1 to 5090)
  AUTHORS   Keeton,T.P., Burk,S.E. and Shull,G.E.
  TITLE     Alternative splicing of exons encoding the calmodulin-binding
            domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1,
            2, 3, and 4
  JOURNAL   J Biol Chem 268 (4), 2740-2748 (1993)
   PUBMED   8428948
REFERENCE   8  (bases 1 to 5090)
  AUTHORS   Brandt,P. and Neve,R.L.
  TITLE     Expression of plasma membrane calcium-pumping ATPase mRNAs in
            developing rat brain and adult brain subregions: evidence for
            stage-specific expression
  JOURNAL   J Neurochem 59 (4), 1566-1569 (1992)
   PUBMED   1328526
REFERENCE   9  (bases 1 to 5090)
  AUTHORS   Burk,S.E. and Shull,G.E.
  TITLE     Structure of the rat plasma membrane Ca(2+)-ATPase isoform 3 gene
            and characterization of alternative splicing and transcription
            products. Skeletal muscle-specific splicing results in a plasma
            membrane Ca(2+)-ATPase with a novel calmodulin-binding domain
  JOURNAL   J Biol Chem 267 (27), 19683-19690 (1992)
   PUBMED   1388171
REFERENCE   10 (bases 1 to 5090)
  AUTHORS   Greeb,J. and Shull,G.E.
  TITLE     Molecular cloning of a third isoform of the calmodulin-sensitive
            plasma membrane Ca2+-transporting ATPase that is expressed
            predominantly in brain and skeletal muscle
  JOURNAL   J Biol Chem 264 (31), 18569-18576 (1989)
   PUBMED   2530223
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JACYVU010000491.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_133288.3.
            
            On Nov 27, 2020 this sequence version replaced NM_133288.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760433
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               JACYVU010000491.1  282788-283074
            288-570             JACYVU010000491.1  299243-299525
            571-903             JACYVU010000491.1  303066-303398
            904-1101            JACYVU010000491.1  309130-309327
            1102-1359           JACYVU010000491.1  309427-309684
            1360-1485           JACYVU010000491.1  310129-310254
            1486-1611           JACYVU010000491.1  310782-310907
            1612-1776           JACYVU010000491.1  315514-315678
            1777-1991           JACYVU010000491.1  316300-316514
            1992-2234           JACYVU010000491.1  317073-317315
            2235-2476           JACYVU010000491.1  318130-318371
            2477-2711           JACYVU010000491.1  323122-323356
            2712-2891           JACYVU010000491.1  325952-326131
            2892-2979           JACYVU010000491.1  326254-326341
            2980-3086           JACYVU010000491.1  326745-326851
            3087-3278           JACYVU010000491.1  327912-328103
            3279-3492           JACYVU010000491.1  330412-330625
            3493-3704           JACYVU010000491.1  330906-331117
            3705-3812           JACYVU010000491.1  332318-332425
            3813-3995           JACYVU010000491.1  334781-334963
            3996-4149           JACYVU010000491.1  339630-339783
            4150-5090           JACYVU010000491.1  349885-350825
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq37"
     gene            1..5090
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /note="ATPase plasma membrane Ca2+ transporting 3"
                     /db_xref="GeneID:29599"
                     /db_xref="RGD:621304"
     exon            1..287
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            288..570
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            571..903
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     CDS             696..4175
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /EC_number="7.2.2.10"
                     /note="plasma membrane calcium ATPase; plasma membrane
                     calcium pump; ATPase, Ca++ transporting, plasma membrane
                     3"
                     /codon_start=1
                     /product="plasma membrane calcium-transporting ATPase 3"
                     /protein_id="NP_579822.1"
                     /db_xref="GeneID:29599"
                     /db_xref="RGD:621304"
                     /translation="MGDMANSSIEFHPKPQQQREVPHVGGFGCTLAELRSLMELRGAE
                     ALQKIQEAYGDVSGLCRRLKTSPTEGLADNTNDLEKRRQIYGQNFIPPKQPKTFLQLV
                     WEALQDVTLIILEVAAIVSLGLSFYAPPGEESEACGNVSGGAEDEGEAEAGWIEGAAI
                     LLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRNGQLLQVPVAALVVGDIAQ
                     VKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSADKDPMLLSGTHVMEGSGRMVV
                     TAVGVNSQTGIIFTLLGAGGEEEEKKDKKAKKQDGAVAMEMQPLKSAEGGEMEEREKK
                     KANVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFVIETFVVDGRVWLAEC
                     TPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETM
                     GNATAICSDKTGTLTTNRMTVVQSYLGDTHYKEIPAPSALTPKILDLLVHAISINSAY
                     TTKILPPEKEGALPRQVGNKTECALLGFILDLKRDFQPVREQIPEDQLYKVYTFNSVR
                     KSMSTVIRMPDGGFRLFSKGASEILLKKCTNILNSNGELRGFRPRDRDDMVKKIIEPM
                     ACDGLRTICIAYRDFSAIQEPDWDNENEVVGDLTCIAVVGIEDPVRPEVPEAIRKCQR
                     AGITVRMVTGDNINTARAIAAKCGIIQPGEDFLCLEGKEFNRRIRNEKGEIEQERLDK
                     VWPKLRVLARSSPTDKHTLVKGIIDSTTGEQRQVVAVTGDGTNDGPALKKADVGFAMG
                     IAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTG
                     ACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLLRKPYGRDKPLISRTMMKN
                     ILGHAVYQLTIIFTLLFVGELFFDIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEIN
                     ARKIHGERNVFDGIFSNPIFCTIVLGTFGIQIVIVQFGGKPFSCSPLSTEQWLWCLFV
                     GVGELVWGQVIATIPTSQLKCLKEAGHGPGKDEMTDEELAEGEEEIDHAERELRRGQI
                     LWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNLSTPTHVTLSAAKP
                     TSAAGNPSGESIP"
     exon            904..1101
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1359
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1485
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1611
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1776
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1991
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2234
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2476
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2711
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            2712..2891
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..2979
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            2980..3086
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            3087..3278
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            3279..3492
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            3493..3704
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            3705..3812
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            3813..3995
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            3996..4149
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
     exon            4150..5090
                     /gene="Atp2b3"
                     /gene_synonym="Pmca3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caccaccagc tccctccctc ccccacccct tcctccctcc ctccgcgctc agagctgcgg
       61 ctggagtcga gcgcgcagcg tcgcccgccg ccgccggtgc ggagcgtggg gagcactcgg
      121 ccacagccct cgcctgccgc ggagggtcct gctccgcact gctggccagg ccgcccagtg
      181 cccgggcctt tccgtccttg gagcggatcg ttgctcgcag ggagctctcc tgcagcaaca
      241 gcgaaagcta cagcagaggc cgcggtggcg gctgtgccca tgccgggcag ccaagagtca
      301 ctcaaaaggg cctcccctca actgatgccc tctatctgtc ctgtggcctg ttgggtgaat
      361 gaagatgagg aaagtttgag tagccacacc ttctggcttc atgctgtgca ggatagtgct
      421 gctaccttcc agagctccaa gattttcccc tctgtgaacc aggccccatt cactaatttc
      481 tccagcccca tcgtcatctg ggatagatca ctcagtgcat ttttggcaat tcagtggtca
      541 acaatgtgtc tcaaagagtt aaggatgcag gtgttcacac ttagcttctt tcccaccacc
      601 accttgcctc acctcagcac tgggatcata ggtggccacc cgtccctagc tattactgag
      661 ctgaggctac acctgtgaga aggctgacag acaggatggg tgacatggcg aacagttcca
      721 ttgagttcca ccccaaaccc cagcagcagc gggaagtgcc tcatgtgggt ggctttggat
      781 gcacgctggc agaactacgc agcctcatgg agctccgagg tgctgaggca ctgcagaaga
      841 tccaagaagc ctatggggat gtcagtgggc tgtgtaggag actaaagacc tcacctactg
      901 aaggcctggc agacaacacc aatgacttgg agaaacgcag gcagatctat gggcagaact
      961 tcatccctcc aaagcagccc aagaccttcc tgcagctggt gtgggaagcc ctgcaggacg
     1021 tgactctcat catcctggag gtggctgcta tcgtctccct gggcctctcc ttctatgcac
     1081 cacctggaga ggaaagtgaa gcctgtggga atgtgtctgg tggggcagaa gacgaaggag
     1141 aggccgaggc tggctggata gagggggctg ccatcctact ctctgtcatc tgtgtggtgc
     1201 tggtcacagc cttcaatgac tggagcaagg aaaagcagtt ccgaggtctt cagagccgaa
     1261 ttgagcagga acagaagttt actgtcatcc gaaatgggca gctcctccag gtccctgtgg
     1321 cagccctggt ggtaggggac attgcccagg tcaaatacgg agatcttctg cctgccgatg
     1381 gtgtgctcat ccaaggcaat gacctcaaga tcgacgagag ctccctgacc ggcgagtcgg
     1441 accatgtgcg caaatcagca gacaaagatc ctatgctgct ctcaggcact catgtcatgg
     1501 aaggttctgg aagaatggta gtaacagctg ttggtgtgaa ctcccagaca ggcatcatct
     1561 ttacattgct tggggcaggt ggagaggagg aggagaagaa agacaagaaa gctaagaagc
     1621 aggatggggc tgttgccatg gaaatgcagc ccctgaagag tgccgagggt ggggaaatgg
     1681 aggagcggga aaagaagaaa gccaacgtac ccaagaagga gaagtcagtc ctgcaaggga
     1741 agctcacaaa actggctgtg cagattggga aagcaggatt ggtgatgtct gctatcactg
     1801 tcatcattct ggtcctctac tttgtgattg agaccttcgt tgtggatggc cgggtgtggc
     1861 tggcagagtg cacaccagtg tatgtgcagt actttgtgaa gtttttcatt attggagtca
     1921 ctgtgttggt tgtggctgtc cctgagggcc tgcctcttgc tgttactatc tccttggctt
     1981 actctgttaa gaaaatgatg aaggacaata acctggtacg ccacctggat gcctgtgaga
     2041 ccatgggcaa tgccacagcc atctgttctg acaagacagg cacactcacc accaaccgta
     2101 tgacagtggt tcagtcctac ctaggagaca cccactacaa agagattcca gctcccagcg
     2161 ccctgacccc caagatcctc gaccttctgg ttcatgccat ctccatcaac agtgcctaca
     2221 ccaccaaaat tctacctcca gagaaagaag gcgctctccc acgccaagtg ggcaacaaaa
     2281 cagagtgtgc tcttttgggc ttcatcttgg acctgaaacg tgacttccaa cctgtacggg
     2341 aacagatacc agaagatcag ctttacaaag tgtacacctt caactcagtt cgcaagtcca
     2401 tgagcacagt tatccgaatg cctgatggtg gcttccgcct cttcagcaag ggagcctcag
     2461 agatcctgct caaaaagtgt acaaacatct taaacagcaa tggtgaactc cgaggatttc
     2521 gtcctcggga ccgggatgac atggtaaaga agatcattga gcctatggct tgtgatggcc
     2581 tccgcaccat atgcatcgcc tacagggact tctctgctat ccaggaaccg gactgggaca
     2641 atgagaatga ggtggtgggt gaccttacct gcatagctgt cgtgggcatc gaggaccctg
     2701 tgcgacctga ggtccctgaa gccattcgaa aatgccagcg tgctggcatt acagtccgta
     2761 tggtaactgg agataacatc aacactgccc gggctattgc agctaagtgt ggcatcatcc
     2821 agccagggga ggatttcctg tgcctggagg ggaaggaatt caacagaagg attcgaaatg
     2881 agaaaggcga gatagaacag gagaggctgg acaaggtgtg gcccaagctt cgggtgcttg
     2941 cccggtcatc tcccactgat aaacatactc tggtcaaagg cataattgac agcacaaccg
     3001 gtgagcagcg gcaggtggtg gctgtgaccg gggatggcac caatgatgga ccagccctca
     3061 aaaaggcaga tgtgggcttc gccatgggca tcgcaggcac tgatgtggcc aaggaggcct
     3121 ctgacatcat tctgactgat gacaacttca ccagcattgt caaggcggtc atgtggggcc
     3181 gcaatgtcta tgacagcatt tccaagttcc tgcagtttca gttgacagtc aatgtggtag
     3241 ctgtgatcgt ggccttcacg ggtgcctgca ttactcagga ctctcctctc aaagctgtgc
     3301 agatgttgtg ggtgaacttg atcatggaca catttgcctc acttgccctg gcaacggaac
     3361 ccccaactga gtcactgctg ctgcggaagc catatggccg ggacaagcct ctcatctcac
     3421 gcaccatgat gaagaacatc cttggacatg ctgtttacca gcttaccatc atctttactc
     3481 tgctatttgt tggagagctt ttctttgaca ttgacagtgg aaggaatgca cctctgcact
     3541 cgccgccctc agagcactac accatcatct tcaacacatt tgtcatgatg cagcttttca
     3601 atgagatcaa tgctcgaaag atccatggtg agaggaatgt ctttgatggc atcttcagca
     3661 accccatctt ctgtaccatt gtcctgggca cctttggaat tcagattgtc attgtccaat
     3721 ttggagggaa gcccttcagc tgttccccac tgtccacaga acagtggctc tggtgtcttt
     3781 ttgttggtgt tggggagctg gtctggggac aggtcattgc cactatcccc accagccagc
     3841 tcaagtgcct gaaggaagca gggcatgggc ctgggaagga tgagatgact gatgaagagt
     3901 tggcggaagg ggaagaagaa attgaccatg ctgagcgaga gctccgcaga ggccagatcc
     3961 tctggtttcg gggcctcaac cggatccaga cacagatgga ggtagtgagt accttcaaga
     4021 gaagcgggtc atttcagggt gctgtgcgcc ggcggtcttc ggtcctcagc cagctccatg
     4081 acgtaaccaa tctttctacc cctactcacg taactctctc tgccgccaag cccaccagcg
     4141 ctgctggcaa tccgagtggt gaaagcattc cgtagctcgc tttatgaagg cctggagaaa
     4201 ccagaatcca agagttgcat ccataacttc atggcaacac ccgagttttt gatcaatgac
     4261 tacacccaca atattccgct catcgatgac acagatgtgg atgagaatga agagcgcctg
     4321 agggccccac ctcccccacc ccctaaccag aacaacaacg ccatagacag cggcatctac
     4381 ctgaccacgc atgccaccaa gtcagctacc tcttcagcat tctcttccag gcctgggagc
     4441 ccactccaca gcatggagac atccctctaa ccagaacttc tctcagtgtg cgcgtgcgcg
     4501 cgcgcgcaca cacacacaca cacacacaca cactctcaca cacacacata ttctctctca
     4561 cacacacaca tacacataca tgcatgcatg cacatacatt ctctctcttt ctcttacaca
     4621 cacacacaca cacacacacg cgcgcgcgcg cgcgcgcgcg cacagtcaca tgcacccaga
     4681 gaggggaacc cgtgcacctg ccaaagaggg aaatgactaa catggctaaa gactttactt
     4741 atgtggtatt tgcaagaagc caattgcatt caataagagg acagcccact aggcttttta
     4801 ctaggttccc agaaggtggg ggaaggagaa aaaaggaaaa gagagggagg ttctttaccc
     4861 gatgtgaagg aaaaagagag catgagacaa gatacacttc ccaccatttt tctactgatg
     4921 cttctttttt taatgaacac tagttccacc atgtgttcac tatttgggct gtgcaatgcg
     4981 gtaggggcta gctgagccta tttggagctt tgctataccc ataacaaaaa aaaagaaaag
     5041 aaaaagaaag aaagagagaa agaaagaaag aaagaaagaa agaaagaaag
//
